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INDONESIA
Jurnal AgroBiogen
Published by Kementerian Pertanian
ISSN : 19071094     EISSN : 25491547     DOI : -
Core Subject : Agriculture,
Agriculture, Biological Sciences & Forestry
Jurnal AgroBiogen memuat artikel primer dan sekunder hasil penelitian bioteknologi dan sumberdaya genetik tanaman, serangga, dan mikroba pertanian. Jurnal ini diterbitkan tiga kali setahun pada bulan April, Agustus dan Oktober oleh Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumberdaya Genetik Pertanian
Arjuna Subject : -
Articles 8 Documents
 1 
Search results for , issue "Vol 14, No 1 (2018): June" : 8 Documents clear
Genetic Diversity Analysis of 53 Indonesian Rice Genotypes using 6K Single Nucleotide Polymorphism Markers Joko Prasetiyono; Nurul Hidayatun; Tasliah Tasliah
Jurnal AgroBiogen Vol 14, No 1 (2018): June
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jbio.v14n1.2018.p1-10

Abstract

Indonesia is rich in rice genetic resources, however, only a small number has been used in variety improvement programs. This study aimed to determine the genetic diversity of Indonesian rice varieties using 6K SNP markers. The study was conducted at ICABIOGRAD for DNA isolation and IRRI for SNP marker analysis. Genetic materials were 53 rice genotypes consisting of 49 varieties and 4 check genotypes. SNP markers used were 6K loci. Results showed that among the markers analyzed, only 4,606 SNPs (76.77%) were successfully read. The SNP markers covered all twelve rice chromosomes of 945,178.27 bp. The most common allele observed was GG, whereas the least allele was TG. Dendrograms of the 53 rice varieties analyzed with 4,606 SNPs demonstrated several small groups containing genotypic mixtures between indica and japonica rice, and no groups were found to contain firmly indica or japonica type. Structure analysis (K = 2) with value of 0.8 showed that the 53 rice varieties were divided into several groups and each group consisted of 4 japonica, 2 tropical japonica, 46 indica, and 1 aus rice type, respectively. IR64 and Ciherang proved to have an indica genome, while Rojolele has japonica one. Dupa and Hawara Bunar, usually grouped into tropical japonica rice, were classified as indica type, and Hawara Bunar has perfectly 100% indica type. The results of this study indicated that rice classification (indica-japonica) which is usually classified based only on morphological characters, e.g. grain and leaf shapes, is not enough and classification based on SNP markers should be considered for that purpose.
Genetic Diversity Analysis and F2 Population Development for Breeding of Long Juvenile Trait in Soybean Tasma, I Made; Yani, N. P. Mega Gena; Purwaningdyah, Rosliana; Satyawan, Dani; Nugroho, Kristianto; Lestari, Puji; Trijatmiko, Kurniawan R.; Mastur, Mastur
Jurnal AgroBiogen Vol 14, No 1 (2018): June
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jbio.v14n1.2018.p11-22

Abstract

Genetic diversity analysis using molecular markers is an important step for selecting appropriate parents in a soybean breeding program. The aims of this study were to (1) analyze genetic diversity of 29 soybean genotypes assessed with 27 SSR markers for selecting appropriate parents and (2) develop F2 populations to be used for breeding long juvenile (LJ) trait in soybean tobe cultivated in short photoperiod condition. The soybean genotypes used consisted of 11 Indonesian soybean genotypes and 18 genotypes introduced from the USA. F2 populations were developed by crossing Grobogan with three introduced genotypes carrying LJ character. The PIC values of the 27 SSR markers ranged from 0.87 to 0.96. Cluster analysis resulted in three mainclusters at coefficient similarity of 0.76. The five LJ introduced accessions and the nine Indonesian genotypes showed high genetic distances and are useful as parent pairs for developing breeding populations. The F1 progeny phenotypicperformances of the cross far exceeded the performaces of both parents. Three F2 populations were developed by crossing the distantly related soybean genotypes. The F2 populations were verified by using SSR markers and it was found that they segregated in a 1:2:1 ratio confirming the segregation ratio of codominant SSR markers. The F2 populations should be useful for breeding LJ characters to improve soybean productivity in low latitude tropical countries such as Indonesia, which has day length of approximately 12 h all year round.
Construction and Transformation of OsERA1 Gene into Expression Vector and Response of Nipponbare-OsERA1 Transgenic Rice to Drought Stress Tri Joko Santoso; Aniversari Apriana Apriana; Atmitri Sisharmini Sisharmini; Kurniawan Rudi Trijatmiko
Jurnal AgroBiogen Vol 14, No 1 (2018): June
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jbio.v14n1.2018.p23-36

Abstract

Drought stress is a major constrain which could influence rice productivity. Enhanced Response to ABA1 (ERA1) gene encoding a β-subunit farnesyltransferase enzyme plays a role to control sensitivity of the guard cells to abscisic acid (ABA), hence regulating drought stress response in plant species including rice. This study aimed to clone the OsERA1 gene into expression vector, introduce it into rice plant, and confirm the positive OsERA1-rice plants conferring drought tolerance. This study was initiated by isolation of the OsERA1 gene from rice cDNAs and cloned it to an expression vector cassette, pCAMBIA1301. The cassette harboring OsERA1 gene was introduced into rice plant cv. Nipponbare mediated by Agrobacterium tumefaciens strain LBA4404.Putative transgenic lines were detected using PCR and Southern blot analyses to confirm the inserted transgene and the positive lines were assayed their tolerance to drought. The OsERA1 gene was successfully isolated and constructed into expression vector to generate pCAMBIA1301-OsERA1. Introduction of the gene into Nipponbare has produced nine putative transgenic rice lines, of which, six lines harbored OsERA1 gene. Southern blot analysis of sixteen T2 plants from two PCR-positive transgenic lines revealed1–3 copies of transgene were integrated into rice genome of transgenic lines. Five transgenic lines of Nipponbare-OsERA1 showed better response to drought at vegetative phase compared to control in term of recovery ability. At generative phase, the five transgenic lines yielded less unfilled grains compared to control. Overall, the transgenic lines obtained from this study could bepotential candidates for developing rice varieties tolerant to drought. 
Characterization of Profenofos Degrading Bacteria Alina Akhdiya; Wartono Wartono; Eman Sulaeman; I Made Samudra
Jurnal AgroBiogen Vol 14, No 1 (2018): June
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jbio.v14n1.2018.p37-46

Abstract

Bioremediation is an inexpensive, easy, and safe technology to rehabilitate agricultural land which is highly polluted with pesticides. The aims of this study were to isolate and characterize profenofos degrading bacteria isolated from Pangalengan soils. The isolation step was carried out by using spread plate method on Nitrate Mineral Salts (NMS) medium containing 100 ppm profenofos. The isolates were selected based on hypersensitive response (HR) and hemolytic test, and ability of the isolates to use and degrade profenofos. The selected isolates were characterized based on the sequence of 16 rRNA and detection of the α and β subunits of terminal deoxygenase and naphtalene dioxygenase encoded genes. Three isolates (CN26, CN44, and CN86), which could use profenofos as the exclusive C source, could degrade more than 86.75% profenofos containing growth medium. Based on the 16S rRNA sequences, the three isolates were closely related to Stenotrophomonas maltophilia (99%), Comamonas terrigena (99%), and Pseudomonas sp. (80%). Pseudomonas CN44 consistenly showed high profenofos degradation activity of up to 91.2% when grown on NMS medium (pH 6.8) for 72 hours. β subunit dioxygenase encoding gene of the isolates were detected using primers Rf2-F/Rf2-R, but optimation of PCR is still needed to detect the α subunit of the gene. Naphtalene dioxygenase gene was detected only from Pseudomonas CN44 using the primer pair 301f/1099r. Based on its biodegradation capability and molecular characteristics, Pseudomonas CN44 is very potential to be developed as a bioremediating agent of profenofos.
Growth Inhibition of Aspergillus spp. by Endophytic Bacteria Dwi N. Susilowati; Untung Haryono
Jurnal AgroBiogen Vol 14, No 1 (2018): June
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jbio.v14n1.2018.p47-54

Abstract

Aspergillus spp. (A. niger, A. flavus, and A. fumigatus) contaminate food commodities through production of secondary metabolites (mycotoxins) and aspergillosis, and thus pose severe hazard to human and animal health. Hence, the inhibition of mycotoxin-producing fungi on agricultural storage commodities needs to be considered. The aims of this study were to evaluate endophytic bacteria isolated from rice tissues that inhibit Aspergillus spp. growth, as well as to characterize the selected isolates morphologically and biochemically. Dual culture and disk diffusion method tests on 155 endophytic bacteria obtained three isolates, i.e. FB-Endo 65, FB-Endo 73, and FB-Endo 95, which showed inhibition zone from 13 to 17 mm against Aspergillus spp. growth. The inhibition zone and quantity of antifungal compounds increased positively with the length of incubation periods from 0 to 6 days. Antifungal compounds from the three isolates were insoluble in ethyl acetate, but soluble in methanol. The methanol soluble substance(s) from FB-Endo 73 showed higher inhibition zone than that of the other isolates. This result indicated that all three isolates produced strong antifungal activity. Morphological and biochemical identifications of the isolates revealed that all isolates belonged to the genus Bacillus sp. Further studies include identification and production methods of antifungal compounds of those endophytic bacteria and their application on stored seeds.
Front Matter JA Vol 14 No 1 Agrobiogen, Jurnal
Jurnal AgroBiogen Vol 14, No 1 (2018): June
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jbio.v14n1.2018.p%p

Abstract

Back Matter JA Vol 14 No 1 Jurnal Agrobiogen
Jurnal AgroBiogen Vol 14, No 1 (2018): June
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jbio.v14n1.2018.p%p

Abstract

Front Matter JA Vol 14 No 1 Jurnal Agrobiogen
Jurnal AgroBiogen Vol 14, No 1 (2018): June
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jbio.v14n1.2018.p%p

Abstract

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