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INDONESIA
Jurnal AgroBiogen
Published by Kementerian Pertanian
ISSN : 19071094     EISSN : 25491547     DOI : -
Core Subject : Agriculture,
Agriculture, Biological Sciences & Forestry
Jurnal AgroBiogen memuat artikel primer dan sekunder hasil penelitian bioteknologi dan sumberdaya genetik tanaman, serangga, dan mikroba pertanian. Jurnal ini diterbitkan tiga kali setahun pada bulan April, Agustus dan Oktober oleh Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumberdaya Genetik Pertanian
Arjuna Subject : -
Articles 5 Documents
 1 
Search results for , issue "Vol 9, No 1 (2013): April" : 5 Documents clear
Seleksi dan Konfirmasi Alel Gen-gen Hd pada Padi Berumur Genjah dan Produktivitas Tinggi Persilangan Code x Nipponbare Ahmad Dadang; Tasliah Tasliah; Joko Prasetiyono
Jurnal AgroBiogen Vol 9, No 1 (2013): April
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jbio.v9n1.2013.p11-18

Abstract

To support the IP 300 program rice varietieswith both early maturity and high productivity are needed.The objective of the research was to improve those traits inCode variety using RIL (recombinant inbreed line) methods.The research was conducted in the year 2009-2011 at thegreenhouse and laboratory of Molecular Biology, IndonesianCenter for Agricultural Biotechnology and GeneticResources Research and Development. Materials consistedof 600 individuals of F2 derived from one of F1 Code xNipponbare crossed plants and they were planted up to F4with seed-to-seed, then confirmed by 8 microsatelitemarkers linked to loci of Hd genes. The molecular analysisshowed that 84 of 600 F2 plants produced. The F4 plants have50% of flowering time shorter than F2 and F3 plants. Twolines (CdNb_388 and CdNb_270) have higher productivitycompared to Code by the number of productive tillers (20)and the number of tiller grains (2240 and 1740) and haveclosely 50% of 60 days flowering time of Nipponbare. Threelines of F4 plants (CdNb_270, 364, and 388) were predictedto have allele of Hd7 gene, and CdNb_472 was predicted tohave alelle of Hd14 gene.
Analisis Kekerabatan 50 Aksesi Kelapa Sawit (Elaeis guineensis Jacq.) Asal Kamerun Berdasarkan Marka Mikrosatelit I Made Tasma; Ahmad Warsun; Dani Satyawan; Syafaruddin Syafaruddin; Budi Martono
Jurnal AgroBiogen Vol 9, No 1 (2013): April
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jbio.v9n1.2013.p19-27

Abstract

Genetic diversity of theIndonesian oil palm collection remains low and collectionenrichness through exploration activities from the center oforigins is required. In 2009, 103 oil palm accessions fromCameroon were collected at the National Oil Palm GeneticResources Collection located at the District of Sijunjung,West Sumatera. The objectives of the present study were toanalayze the 50 Cameroon-originated oil palm accessions inorder to: (1) determine polymorphism levels of the SSRmarkers used; (2) understand diversity levels of the oil palmaccessions tested; and (3) analyze accessions potentiallyused for germ plasm collection. Fifty oil palm accessionswere used in this study. DNA was isolated from leaves of theselected individual plants representing each of theaccessions. DNA was analyzed with 12 SSR markers. Adendrogram was constructed using the UPGMA throughNumerical Taxonomy and Multivariate System programversion 2.1-pc. Results showed that SSR markers useddemonstrated the average number of alleles per locus of 3.6(2-6). The polymorphism level was 0.53 (0.21-0.73). Thephylogenetic analysis resulted nine clusters with geneticdiversity between two accessions ranged from 4-82%. Tenaccessions (20%) showed low genetic diversity (<10%) butthe accessions demonstrated high diversity in floweringtime. Eleven accessions showed medium diversity level (27-42%). Five accessions demonstrated high genetic diversitylevel (45-82%). A confirmation study using more SSRmarkers is recommended. This study finding may be usefulin planning the oil palm germ plasm collection activities.
Perkembangan Aplikasi Teknik Kriopreservasi untuk Konservasi dan Mendukung Program Pemuliaan Tanaman Roostika, Ika
Jurnal AgroBiogen Vol 9, No 1 (2013): April
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jbio.v9n1.2013.p39-48

Abstract

In the beginning, cryopreservationtechnique was primarily used for germplasmslong-term storage as passive collection because cell divisionand metabolism process can be stopped at super lowtemperature, commonly in liquid nitrogen. The technique issuitable for vegetative propagated plants and recalcitrantseeds. Recently, its application is extending for storing manyspecies and orthodox seeds. In this paper, the developmentof cryopreservation application is discussed. In Indonesia,cryopreservation is being studied but the application ofcryopreservation has been significantly developed abroad.The application of cryopreservation technique is not only forpreserving passive collections but also for storing activecollections, including to provide plant materials for hybridization,cellular engineering, genetic transformation, as wellas pathogens eradication or cryotherapy. It is concluded thatcryopreservation plays an important role in conventional andmodern plant breeding program.
Keragaman Genetik Beberapa Genotipe Jarak Pagar (Jatropha curcas L.) Berdaya Hasil Tinggi Berdasarkan Karakter Morfologi, Agronomi, dan Isozim Dewi, Iswari S; Arisanti, Yusie; Purwoko, Bambang S.; Hariyadi, Hariyadi; Syukur, M.
Jurnal AgroBiogen Vol 9, No 1 (2013): April
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jbio.v9n1.2013.p28-38

Abstract

The interest in using Jatropha curcas L. from thefamily Euphorbiaceae for the production of biofuel is rapidlygrowing. The research objective was to determine geneticvariation of several high yielding physic nuts based on theirmorphology, agronomy, and isozyme characters. Theresearch used Completely Randomized Design with threereplications. The treatment was consisted of 8 genotypes i.e.IP-1A, IP-1M, IP-2P, Lombok Timur, Lombok Tengah,Lombok Barat, Sumbawa, and Bima. Analysis of isozyme ofthe eight genotypes was also conducted according to 5enzyme systems, i.e. peroksidase, esterase, aspartataminotransferase, malat dehidrogenase, and alcoholdehidrogenase. Observation was done on qualitative andquantitative characters as well as banding pattern derivedisozymes.The results showed that genetic variation was lowwhen based on qualitative characters and isozyme (0-25%)but relatively high when based on selected quantitativecharacters analysis (17-81%). Analysis of combinedqualitative, quantitative, and isozyme characters still gavelow genetic variation (6-33%). Based on the quantitativecharacters at similarity coefficient of 46% the genotypes canbe devided into three clusters. Improved populationgenotypes, i.e. IP-1A, IP-1M, and IP-2P were placed in 3different clusters, while other genotypes from NTB areawere grouped in the same cluster. Therefore, selectionamong population of the same ecotype based on agronomiccharacters such as yield components, yield and oil contentwas suitable in Jatropha improvement, especially whengenetic variation was low. Furthermore, introduction,mutation and crossing are suggested to increase geneticvariation of current Jatropha collection.
Optimasi Sistem Regenerasi dan Transformasi Padi Varietas Elit Indonesia Aniversari Apriana; Toto Hadiarto; Ahmad Dadang
Jurnal AgroBiogen Vol 9, No 1 (2013): April
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jbio.v9n1.2013.p1-10

Abstract

New ricevariety can be generated by means of transgenic approach.Transgenic rice researches have been conducted in manyinstitutions worldwide using Japonica, Indica, and Javanicavarieties. The most cultivated rice in Indonesia is Indica.Indica type is known to have low responsive tissues inculture and transformation media when compared toJaponica rice. This research activity is aimed to optimizeregeneration and transformation systems of the Indonesianelite rice varieties, so that good method can be achieved tobe used in the generation of transgenic elite rice varieties ofIndica type. The research consisted of two activities:regeneration and transformation optimizations in varieties ofDodokan (upland rice) and Inpari 6 (irrigated rice).Immature embryo was used as the explant in this research.The optimization studies used 2 types of media, NBH (N6salts and vitamins, cassamino acid 0.5 g/l, L-proline 0.5 g/l,sucrose 20 g/l, D-glucose 10 g/l, 2.4-D 2 mg/l, NAA 1 mg/l, BA1 mg/l, agarose Type I 5.5 g/l) and NBH-M (N6 macro salts,B5 micro salts, and vitamins, 0.3 g/l cassamino acid, 3 g/l Lproline,20 g/l sucrose, 3 mg/l 2.4-D, 1 mg/l NAA, 1 mg/l BAP,5.5 agarose Type I), 2 types of regeneration media, R1 (MSbase media and vitamis, 0.3 g/l glutamine, 30 g/l sucrose, 2mg/l kinetin, 1 mg/l NAA, 3 g/l phytagel) and R2 (MS basemedia and vitamins, 2 g/l cassamino acid, 20 g/l sucrose, 30mg/l sorbitol, 2.5 mg/l kinetin, 0.25 mg/l NAA, 3 g/l phytagel).Optimization transformation of Indonesian elite rice varietiesused developed an empty plasmid pCAMBIA 1301 containinghpt gene. The transformation was conducted using twotypes of co-cultivation media, K1 (N6 macro salts, B5 microsalts, and vitamins, 0.5 g/l cassamino acid, 0.5 g/l L-proline,20 g/l sucrose, 10 g/l glucose, 2 mg/l 2.4-D, 1 mg/l NAA, 1mg/l BAP, 0.1 mM acetosyringone) and K2 (N6 macro salts,B5 micro salts, and vitamins, 0.5 g/l cassamino acid, 0.5 g/l Lproline,20 g/l sucrose, 10 g/l glucose, 2 mg/l 2.4-D, 1 mg/lNAA, 1 mg/l BAP, 0.2 mM acetosyringone). The resultsshowed that Inpari 6 could form embryonic calli in NBHmedia and further regenerated well in R1 media (13.8%).The co-cultivation media K1 generated more selected calliwhich then generated green plant of young embryocompared to K2. Inpari 6 showed higher regeneration ratesafter transformation (3.6%) compared to Dodokan (0%).Molecular analysis showed that all 11 transformants (Inpari6) tested contained the hpt gene. These results are expectedto support the development of transgenic Indica ricegeneration in Indonesia.

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