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Buletin Plasma Nutfah
Published by Kementerian Pertanian
ISSN : 14104377     EISSN : 25491393     DOI : -
Core Subject : Science,
Biochemistry, Genetics & Molecular Biology
Buletin Plasma Nutfah (BPN) is an open access scientific journal published by The Indonesian Center for Agricultural Biotechnology and Genetic Resources Research and Development (ICABIOGRAD), The Indonesian Agency for Agricultural Research and Development (IAARD), Ministry of Agriculture. This peer-refereed journal covering the area of genetic resources including exploration, characterization, evaluation, conservation, diversity, traditional knowledge, management and policy / regulation, of all kinds of genetic resources: plants, animals, fishes, insects and microbes. Manuscripts submitted to this journal are those that have never been published in other journals. This journal is published in one volume of two issues per year (June and December). We invite authors to submit the manuscripts to this journal in English or Indonesian. Detail information about the journal, including author guidelines and manuscript template, is available on the website (http://ejurnal.litbang.pertanian.go.id/index.php/bpn). The manuscript should be submitted electronically through our submission system. Buletin Plasma Nutfah (BPN) is an open access scientific journal published by Indonesian Center for Agricultural Biotechnology and Genetic Resources Research and Development (ICABIOGRAD), Indonesian Agency for Agricultural Research and Development (IAARD), Ministry of Agriculture. BPN Accredited by Ministry of Research, Technology and Higher Education of the Republic of Indonesia (No. 21/E/KPTP/2018) for period of 2016-2020. This peer-refereed journal covers the area of genetic resources including exploration, characterization, evaluation, conservation, diversity, traditional knowledge, management and policy / regulation related to genetic resources: plants, animals, fishes, insects and microbes which has never been published in other Journal
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Search results for , issue "Vol 19, No 2 (2013): December" : 6 Documents clear
Seleksi Genotipe Jagung Hibrida Toleran N Rendah nFN Syafrudin; Muhammad Azrai; nFN Suwarti
Buletin Plasma Nutfah Vol 19, No 2 (2013): December
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumberdaya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/blpn.v19n2.2013.p73-80

Abstract

Maize Hybrid Genotypes Selection for Low N Tolerance. Syafruddin, Muhammad Azrai, and Suwarti. Most of the maize crop land development in Indonesia are defficient of N, to overcome these obstacles is generally done with N fertilizer. N fertilization beside increasing the cost of production is also causing environmental pollution. Therefore, fertilizer efficiency is required. One effort to reduce N fertilizer usage is the use of low N-tolerant genotypes. Selection of low Ntolerant maize hybrids are expected to found a low N genotypes with high productivity. The experiment was conducted in low land in May-August 2012 in the Bontobili Research Station, Gowa, South Sulawesi. A total of 32 genotypes including check entries were 4 known hybrid Bima- 2, Bima-3, Bima-4, and Bisi-2 were screened in normal rate of N application (200 kg N/ha) and low N (100 kg N/ha). The treatment used experimental randomized complete block design at each fertilization with three replications. To Determine of genotype x N interaction was used combine analysis. The result showed that in selection of tolerance genotypes for low N, beside yield as selection parameter we can also consider leaf chlorophyll, harvest index, number of kernel in ear and seed weight. Genotypes 1044-0 x 1027-11 and AMB07 x CML 161 were tolerance to low N and B11 x 11 is moderat to low N. Three of genotypes have yield equal to 4 check varieties (Bima-2, Bima-3, Bima-4, and Bisi-2). AbstrakSebagian besar lahan pengembangan tanaman jagung di Indonesia defisien N. Untuk mengatasi kendala tersebut umumnya dilakukan dengan pemupukan N. Pemupukan N di samping meningkatkan biaya produksi juga menyebabkan pencemaran lingkungan. Salah satu upaya untuk menekan penggunaan pupuk N adalah penggunaan genotipe yang toleran N rendah. Seleksi genotipe jagung hibrida diharapkan memperoleh genotipe toleran N rendah dengan produktivitas tetap tinggi. Penelitian dilaksanakan di lahan sawah pada Mei- Agustus 2012 di KP Bontobili, Gowa, Sulawesi Selatan. Sebanyak 32 genotipe termasuk empat pembanding Bima-2, Bima-3, Bima-4, dan Bisi-2 diuji pada takaran pupuk N normal (200 kg N/ha) dan N rendah (100 kg N/ha). Penelitian menggunakan rancangan kelompok pada masing-masing pemupukan dengan tiga ulangan. Untuk mengetahui interaksi antara genotipe dengan N dilakukan analisis gabungan. Hasil penelitian menunjukkan bahwa untuk mendapatkan genotipe jagung toleran N rendah yang akurat di samping mempertimbangkan hasil biji, juga mempertimbangkan komponen lainnya, seperti klorofil daun, indeks panen, jumlah biji per tongkol, dan bobot biji. Genotipe 1044-9 x 1027-11 dan AMB07 x CML161 toleran N rendah dan B11 x 11 moderat terhadap N rendah, ketiga genotipe tersebut mempunyai hasil biji setara dengan varietas pembanding Bima-2, Bima-3, Bima- 4, dan Bisi-2.
Penampilan Genotipe Jagung Berumur Genjah pada Pemupukan N Dosis Tinggi dan Rendah di Lahan Sawah setelah Padi di Sulawesi Selatan nFN Ruchjaniningsih; Muh. Thamrin
Buletin Plasma Nutfah Vol 19, No 2 (2013): December
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumberdaya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/blpn.v19n2.2013.p81-88

Abstract

Genotype Performance of Early Maturity Maize with N High and Low Dosage Fertilization in Irrigated Land After Rice Planting in South Sulawesi. Ruchjaniningsih and Muh. Thamrin. To reduce the negative impact of excessive fertilizer N use efficiency of N needs to be done, especially on maize that much need N. N use efficiency can be improved through the establishment of adaptive varieties of maize N fertilization is low, it is necessary for the characterization of the plant as a basis for screening genotypes. Some strains of maize have been evaluated wetland in Bontomanai, Bantaeng district, South Sulawesi, on May to December 2009, the result of the appearance of phenotypic characters and yield components. The experiments were arranged in a randomized block design with a factorial treatment genotype A (X01904 ), B (X02804 ), C (X02904 ), D (X03404), E (X03604), F (Bima- 1), G (Lamuru), H (Gumarang), and local B yellow for comparison with N fertilization (high and low dose) repeated three times. The results showed that fertilizer N 400 kg/ha had a greater impact on the character of the observed fertilization N 200 kg/ha. Genotypes that have high yield potential in rice fields is Lamuru cultivars (13.44 t/ha) and X01904 (13.33 t/ha). All genotypes tested from early maturing (76-84 dap). Genotype X01904, X02804, X02904, X03404, and Bima-1 excel in the characters observed compared to check varieties. Check varieties (local) is superior genotypes were tested on plant height and ear height layout. AbstrakUntuk mengurangi dampak negatif pemupukan N yang berlebihan perlu dilakukan efisiensi penggunaan N, terutama pada tanaman jagung yang banyak membutuhkan N. Efisiensi penggunaan N dapat ditingkatkan melalui pembentukan varietas jagung adaptif pemupukan N rendah, untuk itu perlu dilakukan karakterisasi tanaman sebagai dasar skrining genotipe. Beberapa galur jagung telah dievaluasi pada lahan sawah di Bontomanai, Kabupaten Bantaeng, Sulawesi Selatan, pada Mei- Desember 2009, untuk melihat penampilan fenotipik karakter hasil dan komponen hasil. Percobaan ditata dalam rancangan acak kelompok pola faktorial dengan perlakuan genotipe A (X01904), B (X02804), C (X02904), D (X03404), E(X03604), F (Bima-1), G (Lamuru), H (Gumarang), dan lokal B Kuning sebagai pembanding dengan pemupukan N (dosis tinggi dan rendah) diulang tiga kali. Hasil penelitian menunjukkan bahwa pemupukan N 400 kg/ha berpengaruh lebih baik terhadap karakter yang diamati dari pemupukan N 200 kg/ha. Genotipe yang mempunyai potensi hasil tinggi di lahan sawah adalah kultivar Lamuru (13,44 t/ha) dan X01904 (13,33 t/ha). Semua genotipe yang diuji berumur genjah (76-84 hst). Genotipe X01904, X02804, X02904, X03404, dan Bima-1 unggul dalam karakter-karakter yang diamati dibandingkan dengan varietas pembanding. Varietas pembanding (lokal) lebih unggul dibandingkan dengan genotipe yang diuji pada karakter tinggi tanaman dan tinggi letak tongkol.
Respon Ketahanan Beberapa Varietas Gandum terhadap Hama Gudang Sitophilus zeamais (Coleoptera: Dryophthoridae) Lina Herlina; Bonjok Istiaji
Buletin Plasma Nutfah Vol 19, No 2 (2013): December
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumberdaya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/blpn.v19n2.2013.p89-101

Abstract

Resistance Response of Several Wheat Varieties Against Pest of Stored Product, Sitophilus zeamais (Coleoptera: Dryophthoridae). Lina Herlina and Bonjok Istiaji. Since Sitophilus zeamais still become the major pest of wheat as stored product. Twelve varieties of wheat were evaluated for their resistance to the infestation of S. zeamais. The study was conducted at the Laboratory of Insect Specimen Collection of Indonesian Center for Agriculture Biotechnology and Genetic Resource Research and Development (ICABIOGRAD) from September 2011 to February 2012. Grains of each 12 varieties of wheat was weighed for 5 grams and placed into a plastic bottle. Six female imagos of S. zeamais (1 week old) were introduced into the bottle contained 5 grams of wheat grains. After seven days, all the insects were removed. Observation were done to count the dead and life insects after introduction. The number of larvae emerged from eggs were calculated and weighed daily. Grain weight of each variety was also weighed, the broken and intact seeds were counted and recorded at the end of the infestation. Research were conducted in randomly complete design using one factor, that was twelve varieties of wheat each were replicated three times. The result showed that the highest mortality of the pest were in Perdix, while the lowest were that in Anemos, Combi, and Nandu (0-16.7%). The highest fertility of the pests were found in the Anemos and SW Triso, while the lowest were there in Picallo (14.33-47.67 insects). Anemos was known to be variety with the highest insects population weight, while the Picallo was the variety that inhibited insect population (0.0161-0.0544 g). The longest of insect development periods was on Combi, the shortest was on Sweta (51.33-64.33 days). The shortest median development time was in the Anemos and the longest was in the Sit Nortrend (37.33-44 days). The highest percentage of seed damage and yield loss were in the Anemos, while the lowest were Picallo and Madonna. ANOVA at 5% showed no significant different for all parameters observed on the tested varieties. Resistance classification based on the indexes of Modification, Pointe and Dobie recorded that the most resistant varieties were Picallo and Pasadena. AbstrakSitophilus zeamais masih menjadi hama utama pada produk pascapanen. Penelitian dilakukan di Laboratorium Koleksi Spesimen Serangga BB Biogen dari September 2011 sampai Februari 2012 untuk mengevaluasi ketahanan 12 varietas gandum terhadap infestasi S. zeamais. Biji 12 varietas masingmasing ditimbang 5 g dan dimasukkan ke dalam botol plastik, kemudian diintroduksikan enam imago betina S. zeamais berumur seminggu. Setelah tujuh hari, seluruh serangga dikeluarkan, dihitung yang mati dan yang masih hidup. Jumlah larva (progeni F1) yang muncul dari telur diamati dan dihitung setiap hari. Berat gandum tiap varietas ditimbang pada akhir percobaan. Biji yang berlubang maupun yang masih utuh dihitung dan dicatat. Penelitian menggunakan rancangan acak lengkap satu faktor, yaitu jenis varietas gandum sebanyak 12 varietas, masing-masing dengan tiga ulangan. Hasil percobaan menunjukkan bahwa mortalitas larva tertinggi ditemukan pada varietas Perdix, sedangkan terendah pada Anemos, Combi, dan Nandu (kisaran 0-16,7%). Fertilitas tertinggi ditemukan pada Anemos dan SW Triso, sedangkan terendah pada Picallo (kisaran 14,33-47,67 ekor). Bobot tertinggi larva serangga ditemukan pada Anemos, terendah pada Picallo (kisaran 0,0161- 0,0544 g). Perkembangan serangga terlama ditemukan pada Combi dan terpendek pada varietas Sweta (kisaran 51,33-64,33 hari). Waktu paruh terpendek pada varietas Anemos dan terpanjang pada varietas Sit Nortrend (kisaran 37,33-44 hari). Kerusakan biji maupun kehilangan hasil tertinggi pada varietas Anemos, sedangkan terendah pada Picallo dan Madonna. Hasil ANOVA pada taraf 5% menunjukkan tidak terdapat perbedaan pengaruh yang signifikan dari seluruh varietas terhadap semua parameter yang diamati. Berdasarkan indeks Modif, Pointe maupun Dobie, varietas yang paling tahan adalah Picallo dan Pasadena.
Potensi Jenis Dipterocarpus retusus Blume di Kawasan Hutan Situ Gunung Sukabumi Titi Kalima; Marfu'ah Wardani
Buletin Plasma Nutfah Vol 19, No 2 (2013): December
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumberdaya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/blpn.v19n2.2013.p102-110

Abstract

Potential of Dipterocarpus retusus Blume Species in Situ Gunung Forest, Sukabumi. Titi Kalima and Marfu'ah Wardani. Analysis of the potential of Dipterocarpus retusus Blume species in Situ Gunung forest, Sukabumi, West Java, carried out in 2010 in a village location Kadudampit, Kadudampit districts, with the goal of uncovering potential and habitat conditions D. retusus in Situ Gunung forest. Data collection using the Systematic Sampling Strip with units such examples observation of 50 m x 20 m. The results showed that D. retusus recorded five induvidu stems of tree, the saplings (four individu) and seedlings (11 individu) at an altitude of 864-865 m above sea level, slope of 45%, and the soil pH from 6.0 to 6.9. The potential of D. retusus diameter rods >10 cm are 50 stems per ha with 35,061 volume m3/ha. The level distribution D. retusus low, grow clustered and sparse. Abundance values for trees 2.50 stems/ha, 2.00 stems/ha for sapling and 2.20 seedling/ha. AbstrakAnalisis potensi jenis Dipterocarpus retusus Blume di kawasan hutan Situ Gunung, Sukabumi, Jawa Barat dilakukan pada tahun 2010 di lokasi Desa Kadudampit, Kecamatan Kadudampit, dengan tujuan mengungkap potensi dan kondisi habitat D. retusus di kawasan hutan Situ Gunung. Pengumpulan data dengan menggunakan metode Systematic Strip Sampling dengan unit contoh berupa jalur pengamatan 50 m dan lebar 20 m. Hasil penelitian menunjukkan bahwa D. retusus tercatat lima induvidu batang tingkat pohon, tingkat pancang empat batang, dan semai diperoleh 11 batang pada ketinggian 864- 865 m dpl, kemiringan 45%, dan pada pH tanah 6,0-6,9. Potensi D. retusus berdiameter batang >10 cm adalah 50 batang/ha dengan volume pohon sebesar 35,061 m3/ha. Tingkat persebaran D. retusus rendah, tumbuh mengelompok dan jarang. Nilai kelimpahan untuk pohon 2,50 batang/ha, pancang 2,00 batang/ha, dan semai 2,20 batang/ha. 
Uji Ketahanan Galur-galur Harapan Padi terhadap Penyakit Hawar Daun Bakteri (Xanthomonas oryzae pv. oryzae) Ras III, IV, dan VIII Siti Yuriyah; Dwinita W. Utami; Ida Hanarida
Buletin Plasma Nutfah Vol 19, No 2 (2013): December
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumberdaya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/blpn.v19n2.2013.p53-60

Abstract

Resistance Test of Promising Rice Lines Against Bacterial Leaf Blight (Xanthomonas oryzae pv. oryzae) Race III, IV, and VIII. Siti Yuriyah, Dwinita W. Utami, and Ida Hanarida. Development of new superior rice varieties resistant to bacterial leaf blight (BLB) has been conducted through utilizing of a wide rice germplasm, from crossing between IR64 and Oryza rufipogon. The aim of this study is to get promising rice lines that resistant to BLB race III, IV, and VIII. The experiments were conducted at greenhouse and Laboratory of Molecular Biology, ICABIOGRAD Bogor, using of 13 promising rice lines that have different levels of resistance to inoculum from pure cultures of BLB race III, IV and VIII. Of these 13 rice lines, six lines showed resistance to race III (Bio5-AC-Blas/BLB-03, Bio62-AC-Blas/BLB-03, Bio111-BC-PIR7, Bio129-BC-WBC, Bio148-Mamol, and Bio154-Mamol-Dro), one line showed resistance to race IV (Bio154-Mamol-Dro), and one line showed resistance to race VIII (Bio5-AC-Blas/BLB-03), with severity rate 1.8 to 8.1%. Of these improve lines Bio5-AC-Blas/BLB-3 and Bio 111-BCPir- 7, were released as new rice varieties, namely Inpari HDB and Inpari Blas, respectively. AbstrakPerakitan varietas padi unggul baru tahan terhadap hawar daun bakteri (HDB) terus dilakukan melalui pemanfaatan sumber daya genetik yang luas. Saat ini telah dirakit galur-galur unggul yang berasal dari persilangan IR64 dan padi liar Rupifogon. Penelitian ini bertujuan untuk mengevaluasi ketahanan galur harapan padi terhadap penyakit HDB dari Ras III, IV, dan VIII. Percobaan dilakukan di rumah kaca dan Laboratorium Kelti Biologi Molekuler, BB Biogen, Bogor. Materi genetik yang digunakan adalah 13 galur harapan padi dan biakan murni dari Ras III, IV, dan VIII. Metode inokulasi dengan pengguntingan dan skoring ketahanan dilakukan sesuai dengan sistem penilaian dari Yashitola et al. (1997). Dari 13 galur padi harapan yang diuji, diperoleh enam galur tahan terhadap Ras III (Bio5-AC-Blas/BLB-03, Bio62-AC-Blas/BLB-03, Bio111- BC-PIR7, Bio129-BC-WBC, Bio148-Mamol-Dro, dan Bio154-Mamol-Dro), satu galur tahan terhadap Ras IV (Bio154-Mamol-Dro) dan satu galur tahan terhadap Ras VIII (Bio5-AC-Blas/BLB-03), dengan tingkat keparahan sebesar 1,8-8,1%. Dalam perkembangannya, dua dari galur harapan, yaitu galur Bio5-AC-Blas/BLB-03 dan Bio111-BC-Pir-7 disetujui untuk dilepas sebagai varietas Inpari HDB dan Inpari Blas.
Pembentukan Koleksi Inti Plasma Nutfah Padi Tiur S. Silitonga; Andari Risliawati
Buletin Plasma Nutfah Vol 19, No 2 (2013): December
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumberdaya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/blpn.v19n2.2013.p61-72

Abstract

Development of Rice Core Collection. Tiur S. Silitonga and Andari Risliawati. Rice genetic resources have been collected from almost all of the district in the provinces of Indonesia. Presently, the collection of rice genetic resourses are totally 4.200 accessions. The purpose of these activities were to test, select and grouping of rice genetic resources to develop core collection. Various activities have been conducted such as characterization and selection of rice genetic resources to agronomic performance and yield, evaluation to brown plant hopper and grassy stunt virus and their resistance/tolerance to bacterial leaf blight, blast, and drought. Development core collection with various traits such as morphology and agronomy characteristics, yield component, seed quality, resistance to pest and diseases and tolerance to abiotic stresses will increase the use of rice genetic resources to create high yielding varieties with various traits. The availability of various rice varieties with traits needs could cover all the areas including lowland, upland, swampy areas, and marginal land areas with endemic attacked by pest and diseases. All rice genetic resources have been characterized to agronomic performance and yield in Sukamandi experimental farm, evaluated their resistance to brown plant hopper and bacterial leaf blight in Sukamandi and Cianjur, and drought tolerance in Jakenan, Central Java. The results of the experiment have developed core collection by grouping varieties with their traits such as 55 accession (accs.) of varieties with long panicle (>30 cm), 25 accs. short duration (<115 days), 32 accs. of dwarf plant (<115 cm), 25 accs. of short to medium duration (<130 days) with yielded 4.7-5.9 t/ha, 35 accs. have low to medium amylose content, 20 accs. resistant to brown plant hopper and grassy stunt virus, 33 accs. resistant to bacterial leaf blight, 23 accs. resistant to blast and 30 accs. tolerant to drought. AbstrakKoleksi plasma nutfah padi saat ini lebih dari 4.200 aksesi yang dikumpulkan dari hampir seluruh provinsi di Indonesia. Untuk meningkatkan pengelolaan dan pemanfaatan sumber daya genetik padi telah dilakukan pengujian, seleksi, dan pengelompokan varietas dalam bentuk koleksi inti. Tersedianya koleksi inti plasma nutfah padi dengan sifat agronomis, morfologis, komponen hasil terutama mutu gabah, kadar amilosa, dan ketahanan terhadap hama dan penyakit akan mempermudah pemanfaatannya dalam perakitan varietas unggul padi umur genjah, produksi tinggi, dengan mutu beras dan rasa nasi yang enak. Penelitian ini bertujuan untuk membentuk koleksi inti dengan cara menyeleksi dan mengelompokkan sumber daya genetik padi pada berbagai sifat penting seperti umur genjah, tanaman pendek, hasil tinggi, toleran terhadap cekaman biotik dan abiotik, bentuk beras, dan kadar amilosa. Tersedianya beragam varietas dalam koleksi inti dengan berbagai sifat yang diinginkan akan mempermudah akses dan pemanfaatan oleh para pengguna, terutama pemulia, dalam perakitan varietas baik padi sawah, padi gogo, dan padi lahan rawa pasang surut. Hampir seluruh plasma nutfah padi telah dikarakterisasi dan diseleksi terhadap sifat agronomis dan hasil di KP Sukamandi. Seleksi terhadap hama wereng coklat dan penyakit hawar daun bakteri dilakukan di Sukamandi dan Cianjur, dan pengujian toleransi kekeringan di KP Jakenan, Jawa Tengah. Dari hasil pengujian telah dibentuk koleksi inti plasma nutfah padi dengan sifat penting, antara lain sebanyak 55 aksesi dengan malai panjang (>30 cm), 25 aksesi berumur genjah (<115 hari), 32 aksesi tanaman pendek (<115 cm), 25 varietas memiliki potensi hasil antara 4,7-5,9 t/ha dan umur genjah sampai sedang (<130 hari), 35 aksesi kadar amilosa rendah sampai sedang, 20 aksesi tahan terhadap hama wereng coklat dan virus kerdil rumput, 33 aksesi tahan terhadap penyakit hawar daun bakteri, 23 aksesi tahan penyakit blas, dan 30 aksesi toleran kekeringan.

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