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Buletin Plasma Nutfah
Published by Kementerian Pertanian
ISSN : 14104377     EISSN : 25491393     DOI : -
Core Subject : Science,
Biochemistry, Genetics & Molecular Biology
Buletin Plasma Nutfah (BPN) is an open access scientific journal published by The Indonesian Center for Agricultural Biotechnology and Genetic Resources Research and Development (ICABIOGRAD), The Indonesian Agency for Agricultural Research and Development (IAARD), Ministry of Agriculture. This peer-refereed journal covering the area of genetic resources including exploration, characterization, evaluation, conservation, diversity, traditional knowledge, management and policy / regulation, of all kinds of genetic resources: plants, animals, fishes, insects and microbes. Manuscripts submitted to this journal are those that have never been published in other journals. This journal is published in one volume of two issues per year (June and December). We invite authors to submit the manuscripts to this journal in English or Indonesian. Detail information about the journal, including author guidelines and manuscript template, is available on the website (http://ejurnal.litbang.pertanian.go.id/index.php/bpn). The manuscript should be submitted electronically through our submission system. Buletin Plasma Nutfah (BPN) is an open access scientific journal published by Indonesian Center for Agricultural Biotechnology and Genetic Resources Research and Development (ICABIOGRAD), Indonesian Agency for Agricultural Research and Development (IAARD), Ministry of Agriculture. BPN Accredited by Ministry of Research, Technology and Higher Education of the Republic of Indonesia (No. 21/E/KPTP/2018) for period of 2016-2020. This peer-refereed journal covers the area of genetic resources including exploration, characterization, evaluation, conservation, diversity, traditional knowledge, management and policy / regulation related to genetic resources: plants, animals, fishes, insects and microbes which has never been published in other Journal
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Articles 5 Documents
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Search results for , issue "Vol 20, No 2 (2014): December" : 5 Documents clear
Selection Resistance of Rice Germplasm Accessions to Bacterial Leaf Blight Dini Yuliani; Rina H. Wening; Sudir Sudir
Buletin Plasma Nutfah Vol 20, No 2 (2014): December
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumberdaya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/blpn.v20n2.2014.p65-76

Abstract

Seleksi Ketahanan Aksesi Plasma Nutfah Padi terhadap Hawar Daun Bakteri. Dini Yuliani, Rina H. Wening, dan Sudir. Usaha budi daya tanaman padi di Indonesia selalu dihadapkan pada berbagai kendala, di antaranya serangan penyakit hawar daun bakteri (HDB) yang disebabkan oleh bakteri Xanthomonas oryzae pv. oryzae (Xoo). Pengendalian penyakit HDB dengan varietas tahan merupakan salah satu teknik yang murah dan mudah dilakukan oleh petani padi. Aksesi plasma nutfah sebagai sumber tetua untuk perakitan varietas tahan perlu diketahui reaksi ketahanannya terhadap penyakit HDB. Penelitian ini bertujuan untuk menyeleksi ketahanan aksesi plasma nutfah terhadap penyakit HDB patotipe III, IV, dan VIII. Penelitian dilaksanakan di Kebun Percobaan Balai Besar Penelitian Tanaman Padi di Sukamandi, Subang, Jawa Barat pada Musim Hujan 2012/2013 dan Musim Kemarau 2013 dengan Rancangan Acak Terpisah. Petak utama adalah tiga patotipe Xoo, yaitu patotipe III, IV, dan VIII. Sedangkan anak petak adalah materi yang diuji, yaitu 20 aksesi plasma nutfah dan tiga varietas pembanding. Sebanyak 20 rumpun tanaman per petak diinokulasi Xoo dengan metode pengguntingan. Inokulasi dilakukan pada saat pertanaman menjelang stadium primordia. Ujung-ujung daun digunting sepanjang kira-kira 10 cm dari ujung daun dengan gunting inokulasi yang berisi suspensi bakteri Xoo umur 48 jam dengan kepekatan 108 cfu. Pengamatan keparahan penyakit HDB dilakukan dengan mengukur panjang gejala terpanjang pada umur dua, tiga, dan empat minggu setelah inokulasi. Hasil pengujian ketahanan terhadap HDB patotipe III, IV, dan VIII diperoleh tiga aksesi plasma nutfah yang berasal dari galur isogenik menunjukkan keparahan penyakit HDB tidak berbeda nyata dengan varietas pembanding tahan Angke pada dua musim tanam. Galur isogenik tersebut, yaitu IRBB 55, IRBB 60, dan IRBB 61. Ketiga galur isogenik tersebut dapat dijadikan tetua tahan dalam perakitan varietas unggul baru tahan HDB. AbstrakRice cultivation in Indonesia has been faced with many obstacles, including the attack of bacterial leaf blight (BLB) that caused by bacteria Xanthomonas oryzae pv. oryzae (Xoo). Resistant variety was considered as the cheap technique to control BLB disease and could be used by rice farmers. Germplasm accessions as a source to build resistant varieties must be known their resistance to BLB disease. This study aimed at selecting the resistance germplasm accessions to BLB pathotype III, IV, and VIII. The study was conducted at the Experimental Station of the Indonesian Center for Rice Research in Sukamandi, Subang, West Java at wet season 2012/2013 and dry season 2013 with Split Plot Design. The main plot was three pathotypes Xoo i.e. pathotype III, IV, and VIII. The subplot was 20 germplasm accessions and three check varieties. A total of 20 hills of rice plants per plot were inoculated by Xoo with cutting method. Inoculation was conducted before the primordia stage. Inoculation of bacterial suspension containing Xoo aged 48 hours at a concentration of 108 cfu. Observations BLB disease severity was done by measuring the longest length of symptoms at the age of two, three, and four weeks after inoculation (WAI). The result showed that three germplasm accessions from near isogenic lines were not significantly different with the check varieties (Angke) in their resistance to Xoo pathotype III, IV, and VIII in two cropping seasons. The isogenic lines were IRBB 55, IRBB 60, and IRBB 61 can be used in the assembly of resistant new varieties to BLB.
Evaluasi Hibrid Hasil Persilangan Mangga Arumanis-143 dengan Tiga Kultivar Mangga Merah Berdasarkan Karakter Buah Karsinah Karsinah; Rebin Rebin; Lukitariati Sadwiyanti
Buletin Plasma Nutfah Vol 20, No 2 (2014): December
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumberdaya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/blpn.v20n2.2014.p77-84

Abstract

Evaluation of Mango Hybrids Derived from Crossing of Arumanis-143 with Three Red Mango Cultivars Based on Fruit Characters. Karsinah, Rebin, and Lukitariati Sadwiyanti. In order to improve the character of mango cv. Arumanis-143 that has green-skinned fruit to become red one, Indonesian Tropical Fruits Research Institute Solok had mainactivity, i.e. crossing between Arumanis-143 with Cukurgondang red clones. The objectives of this research were to evaluate the hybrids of mango cv. Arumanis-143 crossing with three red mango cultivars based on fruit characters, and to get 1–2 new superior varieties candidate that had taste like Arumanis-143 with red/attractive fruit skin color. The research was conducted at Cukurgondang Experimental Field, Pasuruan, East Java from June to December 2011. Plant materials used were four cultivars of parents i.e. Arumanis- 143, Irwin, Gedong Gincu, and Saigon, and 27 hybrids from crossing between Arumanis-143 with Irwin, Arumanis-143 with Gedong Gincu, and Arumanis-143 with Saigon. The results indicated that there were 10 hybrids of 27 hybrids having bear fruits i.e. four hybrids from crossing between Arumanis-143 with Irwin, four hybrids from crossing between Arumanis-143 with Gedong Gincu, and two hybrids from crossing between Arumanis-143 with Saigon. The fruit shape of four hybrids from crossing between Arumanis-143 with Irwin were elliptic, fruits size varies with the smallest fruit weigh was F1-09 i.e. 202 g and the largest was F1-14 i.e. 443 g, fruits taste were sourish sweet-sweet, and the fruits skin color were green-yellow. Fruit shape of four hybrids from crossing between Arumanis-143 with Gedong Gincu were elliptic, fruit size varies with the smallest fruit weigh was F1-87 i.e. 281 g and the largest was F1-18 i.e. 519 g, fruits taste were vapidness sweet-very sweet, the fruits skin color were green-orangish green. The fruit shape of two hybrids from crossing between Arumanis-143 with Saigon were elliptic, fruit weigh of F1-45 was 141 g, fruit taste was sweet with yellowish-red fruit skin color and F1-49 was 232 g, fruit taste was sweet with yellowish green fruit skin color. Result of hibrids evaluation indicated that there was a new superior variety candidate that has taste like Arumanis-143 with yellowish-red fruit skin color. AbstrakDalam rangka memperbaiki karakter buah mangga Arumanis- 143 yang kulit buahnya berwarna hijau agar menjadi merah, Balai Penelitian Tanaman Buah Tropika, Solok, telah menyilangkan antara mangga Arumanis-143 dengan klon merah Cukurgondang. Penelitian bertujuan untuk mengetahui karakter hibrid hasil persilangan mangga Arumanis-143 dengan tiga kultivar mangga merah berdasarkan karakter buah dan untuk mendapatkan 1–2 kandidat varietas unggul baru yang mempunyai cita rasa seperti Arumanis-143 dengan kulit buah berwarna merah/menarik. Penelitian dilaksanakan di Kebun Percobaan Cukurgondang, Pasuruan, Jawa Timur, dari bulan Juni sampai Desember 2011. Bahan tanaman yang digunakan ialah empat kultivar tanaman tetua, yaitu Arumanis-143, Irwin, Gedong Gincu, dan Saigon, serta 27 hibrid hasil persilangan Arumanis-143 dengan Irwin, Gedong Gincu, dan Saigon. Hasil penelitian menunjukkan bahwa dari 27 hibrid yang dievaluasi terdapat 10 hibrid yang berbuah, yaitu empat hibrid hasil persilangan antara Arumanis-143 dengan Irwin, empat hibrid hasil persilangan Arumanis-143 dengan Gedong Gincu, dan dua hasil persilangan Arumanis-143 dengan Saigon. Buah dari empat hibrid hasil persilangan antara mangga Arumanis-143 dengan Irwin berbentuk jorong, ukuran buah bervariasi, yang terkecil F1-09 berbobot 202 g dan terbesar F1-14 berbobot 443 g, rasa buah manis asam-manis, kulit buah berwarna hijau-kuning. Buah dari empat hibrid hasil persilangan Arumanis-143 dengan Gedong Gincu berbentuk jorong, ukuran buah bervariasi, yang terkecil F1-87 berbobot 281 g dan terbesar adalah F1-18 berbobot 519 g, rasa buah manis hambar-sangat manis, kulit buah berwarna hijau-hijau orange. Buah dari dua hibrid hasil persilangan Arumanis-143 dengan Saigon berbentuk jorong, buah F1-45 berbobot 141 g, rasa manis dengan kulit buah berwarna merah kekuningan dan buah F1-49 berbobot 232 g, rasa manis dengan kulit buah berwarna hijau kekuningan. Dari hasil evaluasi terhadap hibrid mangga diperoleh satu kandidat varietas unggul baru yang bercita rasa seperti Arumanis-143 dengan kulit buah berwarna merah kekuningan, yaitu F1-45.
Sebaran dan Potensi Keruing (Dipterocarpus spp.) di Pulau Siberut, Sumatera Barat N. M. Heriyanto; M. Bismark
Buletin Plasma Nutfah Vol 20, No 2 (2014): December
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumberdaya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/blpn.v20n2.2014.p85-92

Abstract

Distribution and Potency of Keruing (Dipterocarpus spp.) on the Island of Siberut, West Sumatra. Nur. M. Heriyanto and M. Bismark. Distribution and potency of Dipterocarpus spp. (keruing) were studied in Siberut Biosphere Reserve area, covering Sot Boyak and Bekemen forest village, Siberut National Park, West Sumatra. Six research plots of 50 m x 50 m (0.25 ha) were laid down. The results showed that Dipterocarpus spp. density at the tree level were 31 trees/ha at Sot Boyak and 38 trees/ha at Bekemen forest village with important value index of 63.83% and 77.54% respectively. The strongest association of other trees to Dipterocarpus spp. were Hopea dryobalanoides Miq., Baccaurea bracteata Muell. Arg. and Endospermum diadenum Miq. as reflected respectively by 0.69, 0.67 and 0.56 of Ochiai index. Dipterocarpus spp. were commonly located on the slopes and ridges. The best grouping was on 50-59% Slopes. Slope relationship with the population distribution of Dipterocarpus spp. showed by the equation of Y = 5.83 ln (x) + 3.44 with R2 = 0.90. Regeneration of Dipterocarpus spp. in natural forest is highly dependent on the recruitment population. Saplings recruitment population was 556 individuals/ha, and seedling was 3,434 individual/ha. AbstrakSebaran dan potensi Dipterocarpus spp. (keruing) diteliti di kawasan Cagar Biosfer Siberut yang meliputi hutan Desa Sot Boyak dan Bekemen, Taman Nasional Siberut, Sumatera Barat. Contoh plot penelitian dibuat dengan ukuran 50 m x 50 m (0,25 ha) dengan enam ulangan. Hasil penelitian menunjukkan, kerapatan Dipterocarpus spp. tingkat pohon di hutan Desa Sot Boyak 31 pohon/ha dan Bekemen 38 pohon/ha dengan indeks nilai penting masing-masing 63,8% dan 77,5%. Asosiasi terkuat dengan pohon lain terhadap keberadaan Dipterocarpus spp. adalah Hopea dryobalanoides Miq. indeks Ochiai 0,69, jenis Baccaurea bracteata Muell. Arg. (indeks Ochiai 0,67) dan Endospermum diadenum Miq. (indeks Ochiai 0,56). Habitat Dipterocarpus spp. berada di lereng dan punggung bukit, pengelompokannya terbaik pada kelerengan 50– 59%. Hubungan kemiringan lahan dengan sebaran populasi Dipterocarpus spp. mengikuti persamaan Y = 5,83l n(x) + 3,44 dengan R2 = 0,90. Regenerasi Dipterocarpus spp. di hutan alam sangat bergantung pada populasi anakan. Populasi anakan untuk tingkat belta sebesar di 556 individu/ha, tingkat semai 3.434 individu/ha.
Pendugaan Keragaman Genetik dan Korelasi Antara Komponen Hasil Kacang Hijau Berumur Genjah Ratri Tri Hapsari
Buletin Plasma Nutfah Vol 20, No 2 (2014): December
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumberdaya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/blpn.v20n2.2014.p51-58

Abstract

Estimation of Genetic Variability and Correlation Among Early Maturity Mungbean Yield Components. Ratri T. Hapsari. Early maturity mungbean [Vigna radiata (L.) Wilczek] is very important to avoid drought stress, pest and disease attack as well as increase the index planting. The aims of this research was to estimate genetic variability and correlation. The genetic study included heritability, coefficient of genetic variability, genetic advance and correlation among yield components so that it can be used as selection criteria for early maturity mungbean. A total of 145 accessions of mungbean were tested at Muneng farm station in March-June 2010 using a randomized block design, with two replicates. Each accession was planted at 0.8 m x 4 m with spacing 40 cm x 10 cm, with two plants/hole. Fertilization was done by adding 50 kg urea, 75 kg SP36, and 75 kg KCl/ha, at the time of planting. The results showed that mungbean accesions had significant differences in all characters tested. The genetic variance value of all characters was broad with high broadsense heritability estimates, except for number of pods/ cluster and seed number/pod. Genetic advance of all characters were high, except for seed number/pod. The phenotypic correlation between 1000 seeds weight and pod length with seed yield were positive significant while plant height, flowering days, days to maturity, and number of pods per plant had negative significant correlation with its yield. Therefore, plant height, days to maturity, pod lenght, 1000 seeds weight and seed yield could be used as selection criteria based on estimating value of genetic variability, correlation with yield and economic value. There were five genotype which have index value above 20, i.e MLGV 0353, MLGV 0362, MLGV 0354, MLGV 0358, and MLGV 0351. AbstrakKacang hijau [Vigna radiata (L.) Wilczek] berumur genjah berperan penting untuk menghindari cekaman kekeringan, serangan hama penyakit, dan meningkatkan indeks pertanaman. Penelitian ini bertujuan untuk mengetahui nilai duga parameter genetik dan korelasi antar komponen hasil sehingga dapat digunakan sebagai kriteria seleksi kacang hijau berumur genjah. Sebanyak 145 genotipe kacang hijau diuji di KP Muneng pada bulan Maret sampai dengan Juni 2010 menggunakan Rancangan Acak Kelompok dengan dua ulangan. Setiap aksesi ditanam pada plot 0,8 m x 4 m dengan jarak tanam 40 cm x 10 cm, dua tanaman/lubang. Pemupukan dilakukan dengan 50 kg urea, 75 kg SP36, dan 75 kg KCl per hektar pada saat tanam. Parameter yang diamati adalah tinggi tanaman, umur 50% berbunga, umur 80% masak, jumlah polong/tangkai, jumlah polong/tanaman, panjang polong, jumlah biji/polong, bobot 1.000 biji, dan bobot biji/plot. Hasil penelitian menunjukkan bahwa genotipe yang diuji memiliki keragaman semua sifat yang diamati. Keragaman genetik dan fenotipik tergolong luas. Heritabilitas arti luas tergolong tinggi, kecuali jumlah polong/tangkai dan jumlah biji/polong tergolong sedang. Kemajuan genetik seluruh karakter tinggi, kecuali jumlah biji/polong. Korelasi antara bobot 1.000 biji dan panjang polong bernilai positif nyata dengan bobot biji/plot, sedangkan tinggi tanaman, umur berbunga, umur masak, dan jumlah polong per tanaman berkorelasi negatif nyata. Berdasarkan nilai duga parameter genetik, korelasi antarhasil, dan nilai ekonomisnya, maka tinggi tanaman, umur masak, panjang polong, bobot 1.000 biji dan bobot biji per plot dapat dijadikan kriteria seleksi indeks. Terdapat lima genotipe memiliki nilai indeks lebih dari 20, yaitu MLGV 0353, MLGV 0362, MLGV 0354, MLGV 0358, dan MLGV 0351.
Keragaan Karakter Agronomi Padi Hibrida Baru Nita Kartina; Yuni Widyastuti; Satoto Satoto
Buletin Plasma Nutfah Vol 20, No 2 (2014): December
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumberdaya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/blpn.v20n2.2014.p59-64

Abstract

Agronomic Characters of New Hybrid Rice. Nita Kartina, Yuni Widyastuti, and Satoto. Hybrid rice is the F1 generation derived from the crossing between cytolasmic genetic male sterile and restorer as two parental line. Development of hybrid rice technology obtained throught heterosis This research has the aim was to assess the agronomy characters of some new hybrid rice. Four hundred of Indonesia Center of Rice Research (ICRR) new hybrids rice selected based on yield and phenotipic performances. The activitity conducted in Sukamandi field West Java Province in wet season 2012. The research was arranged in an augmented design that divided in to seven blocks. Each block consist of 20 hybrid rice and five check such as Mapan P.05, Inpari 13, Dodokan, Ciherang, and Hipa 8. There are fourteen hybrids rice showed good agronomic characters and has higher heterosis standard than Ciherang and Hipa 8. Four hybrid rice combinations give the result that significantly different than Ciherang (5.59 t/ha) as check variety. Those hybrids are GMJ11/CRS777 (9.1 t/ha), GMJ12/CRS707 (7.6 t/ha), GMJ13/CRS703 (8.7 t/ha), and GMJ12/CRS795 (7.95 t/ha). AbstrakPadi hibrida merupakan generasi F1 hasil persilangan antara galur mandul jantan sebagai tetua betina dan galur pemulih kesuburan sebagai tetua jantan. Teknologi padi hibrida dihasilkan melalui pemanfaatan heterosis. Penelitian ini bertujuan untuk mengkaji keragaan agronomi sejumlah padi hibrida baru. Sebanyak 400 padi hibrida baru rakitan Balai Besar Penelitian Tanaman Padi diseleksi berdasarkan daya hasil dan penampilan fenotipik di lapangan menggunakan analisis augmented. Penelitian dilakukan di Kebun Percobaan Sukamandi, Jawa Barat, pada MT II tahun 2012 menggunakan lima varietas kontrol, yaitu Mapan P.05, Inpari 13, Dodokan, Ciherang dan Hipa 8 yang ditanam berulang pada tujuh blok dan tiap blok terdiri dari 20 kombinasi padi hibrida. Diperoleh 14 kombinasi padi hibrida yang menunjukkan keragaan yang lebih baik dan memiliki standar heterosis lebih tinggi dari varietas kontrol Ciherang dan Hipa 8. Empat kombinasi hibrida memberikan hasil yang nyata lebih tinggi dibanding Ciherang sebagai varietas pembanding dengan hasil (5,59 t/ha), hibrida tersebut adalah GMJ11/CRS777 (9,1 t/ha), GMJ12/CRS707 (7,6 t/ha), GMJ13/CRS703 (8,7 t/ha), dan GMJ12/CRS795 (7,95 t/ha).

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