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Buletin Plasma Nutfah
Published by Kementerian Pertanian
ISSN : 14104377     EISSN : 25491393     DOI : -
Core Subject : Science,
Biochemistry, Genetics & Molecular Biology
Buletin Plasma Nutfah (BPN) is an open access scientific journal published by The Indonesian Center for Agricultural Biotechnology and Genetic Resources Research and Development (ICABIOGRAD), The Indonesian Agency for Agricultural Research and Development (IAARD), Ministry of Agriculture. This peer-refereed journal covering the area of genetic resources including exploration, characterization, evaluation, conservation, diversity, traditional knowledge, management and policy / regulation, of all kinds of genetic resources: plants, animals, fishes, insects and microbes. Manuscripts submitted to this journal are those that have never been published in other journals. This journal is published in one volume of two issues per year (June and December). We invite authors to submit the manuscripts to this journal in English or Indonesian. Detail information about the journal, including author guidelines and manuscript template, is available on the website (http://ejurnal.litbang.pertanian.go.id/index.php/bpn). The manuscript should be submitted electronically through our submission system. Buletin Plasma Nutfah (BPN) is an open access scientific journal published by Indonesian Center for Agricultural Biotechnology and Genetic Resources Research and Development (ICABIOGRAD), Indonesian Agency for Agricultural Research and Development (IAARD), Ministry of Agriculture. BPN Accredited by Ministry of Research, Technology and Higher Education of the Republic of Indonesia (No. 21/E/KPTP/2018) for period of 2016-2020. This peer-refereed journal covers the area of genetic resources including exploration, characterization, evaluation, conservation, diversity, traditional knowledge, management and policy / regulation related to genetic resources: plants, animals, fishes, insects and microbes which has never been published in other Journal
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Articles 7 Documents
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Search results for , issue "Vol 9, No 2 (2003): Desember" : 7 Documents clear
Sifat Fisik dan Komponen Kimia Bunga Melati Jasminum officinale nFN Suyati; Sulusi Prabawati; nFN Sjaifullah
Buletin Plasma Nutfah Vol 9, No 2 (2003): Desember
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumberdaya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/blpn.v9n2.2003.p19-22

Abstract

AbstractJasminum officinale from Purbalingga (Central Java) was harvested at three stages of maturity: M-1, M-2, and full bloming. Physical and chemical characteristics were determined by weight of flower, size of flower, aroma, and chemical content. Flowers harvested at stage maturity of M-2: colour of cepal was dark violet, there was no aroma, and flowers did not open. At maturity stage of M-1: optimal flower in size, colour of sepal was soft violet, flowers were fully opened, and had aromatic flavor. The maturity stage of M-1 is very useful in selecting flowers for tea industry or essential oil industry. The opened flowers was useful only for spread flower. Dominant component of aromatic flavors was benzil acetat (46.8%) and followed by methyl salisilat (24.4%), Z. jasmone (20.2%), lynalol (2.9%), neurol idol (2.7%), dan indole (1.7%).AbstrakBunga melati Gambir dari Purbalingga (Jawa Tengah) dipanen pada berbagai tingkat ketuaan (M-1, M-2, dan mekar penuh). Bunga setelah dipanen diamati sifat fisiknya, meliputi bobot kuntum, ukuran kuntum, warna bunga, keharuman, dan kandungan minyak atsiri. Hasil penelitian menunjukkan bunga melati Gambir yang dipanen pada tingkat ketuaan M-2 memiliki kuncup berwarna ungu tua, tidak harum, dan tidak dapat mekar. Bunga dengan tingkat ketuaan panen M-1 memiliki ukuran kuntum bunga optimal, berwarna ungu muda, dapat mekar, dan berbau harum. Bunga mekar penuh berwarna putih dan kurang harum. Bunga dengan tingkat kemekaran M-1 dapat digunakan untuk industri teh dan minyak atsiri, sedangkan tingkat kemekaran penuh hanya cocok untuk bunga tabur. Komponen dominan keharuman bunga adalah benzil acetat (46,8%), kemudian diikuti oleh methyl salisilat (24,4%), Z. jasmone (20,2%), lynalol (2,9%), neurol idol (2,7%), dan indole (1,7%).
Pemanfaatan Dedak Padi sebagai Pakan Tambahan Rusa R. Garsetiasih; N. M. Heriyanto; Jaya Atmaja
Buletin Plasma Nutfah Vol 9, No 2 (2003): Desember
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumberdaya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/blpn.v9n2.2003.p23-27

Abstract

AbstractThe experiment was conducted to study growth of deer (Cervus timorensis) fed on mixture of grass, nampong leaves and rice bran. Each of four head deers of 6-8 months old with 22.5-34.5 kgs of weight was consecutively feed on grass with nampong (treatment R0), grass with leaves of nampong and 1% rice bran (treatment R1), grass with leaves of nampong and 1,5% rice bran (treatment R2), grass with leaves of nampong and 2% rice bran (treatment R3). Percentages of the rice bran were based on the average initial weight of the deer. The result showed that the treatment of R2 tended to give better growth than other treatments. Statistical analysis result showed that among the treatment to the deer growth are non significant.AbstrakPenelitian pengaruh pemberian hijauan pakan berupa rumput dan daun nampong dengan campuran dedak padi telah dilakukan untuk mengetahui pertumbuhan rusa Timor (Cervus timorensis). Penelitian menggunakan empat ekor rusa umur 6- 8 bulan dengan bobot badan berkisar antara 22,5-34,5 kg. Perlakuan yang diberikan adalah rumput dan daun nampong (R0), rumput dan daun nampong ditambah 1% dedak padi (R1), rumput dan daun nampong ditambah 1,5% dedak padi (R2), rumput dan daun nampong ditambah 2% dedak padi (R3). Dedak padi diberikan berdasarkan bobot badan awal rusa. Hasil penelitian menunjukkan bahwa perlakuan pemberian rumput dan daun nampong ditambah 1,5% dedak padi (R2) cenderung memberikan pertumbuhan yang lebih baik dibandingkan dengan perlakuan lainnya. Hasil analisis statistik menunjukkan bahwa perlakuan tidak memberikan pengaruh yang nyata terhadap pertumbuhan rusa.
Karakterisasi Ukuran dan Bentuk Umbi Plasma Nutfah Ubi Jalar nFN Sutoro; nFN Minantyorini
Buletin Plasma Nutfah Vol 9, No 2 (2003): Desember
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumberdaya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/blpn.v9n2.2003.p1-6

Abstract

AbstractSweet potato is one of the secondary crops which is mainly used as a source of carbohydrates and contains of some minerals and vitamin. Tuber weight and tuber shape are important characters of sweet potato related to market orientation, and tuber formation is related to difficulty in harvesting by manual tools. Evaluation of sweet potato germplasm was conducted in Pacet, Cianjur, West Java during the year of 2001. Fifty accessions of sweet potato were measured their tuber characteristics five and half month after planting, and found that they have large variability on them. Several accessions had small, medium, and large tuber. There were no correlation between tuber shape, tuber formation, and the variability of tuber weight, tuber number, yields of sweet potato.AbstrakUbi jalar sebagai salah satu palawija berperan penting sebagai sumber karbohidrat, vitamin, dan mineral. Bobot dan bentuk umbi merupakan karakter penting ubi jalar karena berkaitan dengan pemasaran, sedangkan formasi umbi berkaitan dengan kemudahan pemanenan. Evaluasi terhadap 50 plasma nutfah ubi jalar telah dilakukan di Pacet, Cianjur, Jawa Barat, pada MT 2001. Hasil pengamatan menunjukkan adanya keragaman yang cukup besar dari plasma nutfah yang diteliti. Beberapa aksesi memiliki umbi berukuran kecil, sedang, dan besar. Tidak ada hubungan antara bentuk, formasi umbi dengan keragaman bobot, jumlah, dan hasil ubi jalar.
Status Kelangkaan Jenis Pohon di Kelompok Hutan Sungai Lekawai-Sungai Jengonoi, Sintang, Kalimantan Barat N. M. Heriyanto; Endro Subiandono
Buletin Plasma Nutfah Vol 9, No 2 (2003): Desember
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumberdaya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/blpn.v9n2.2003.p28-37

Abstract

AbstrackStudy on the rarity status of plant species in Lekawai river - Jengonoi river forest complex, Sintang, West Kalimantan aimed of confirming rarity status based on IUCN study for further conservation. Stratified random sampling was applied. Fifty five tree species were found. Eleven species were vulnerable based on IUCN criteria such as Dipterocarpus elongatus Foxw., Hopea mengarawan Miq., Shorea macroptera Dyer., Dryobalanops oblongifolia Dyer., Shorea parvifolia Dyer., Agathis borneensis Becc., Eusideroxylon zwageri, Shorea laevis Ridl., Cratoxylum arborescens BL., Alstonia angustifolia Hook.f., and Dacryodes rostrata H.J.L. However based on the field study their species rarity status were not proven. Only Aquilaria malaccensis Lamk. fell under species rarity status and included vulnerable based on IUCN category. Therefore, this species should be conserved.AbstrakPenelitian status kelangkaan jenis pohon di kelompok hutan Sungai Lekawai - Sungai Jengonoi, Sintang, Kalimantan Barat, bertujuan untuk mengetahui status jenis pohon berdasarkan pada IUCN, untuk selanjutnya diambil langkah pelestariannya. Metode yang digunakan, yaitu penarikan contoh bertingkat dengan peletakan satuan contoh tingkat pertama secara terarah dan tingkat kedua secara sistematis. Dalam penelitian ditemukan 55 jenis pohon, 11 di antaranya termasuk kriteria langka IUCN, yaitu Dipterocarpus elongatus Foxw. (keruing), Hopea mengarawan Miq. (meranti batu), Shorea macroptera Dyer. (meranti kuning), Dryobalanops oblongifolia Dyer. (kapur), Shorea parvifolia Dyer., Agathis borneensis Becc. (damar), Eusideroxylon zwageri (ulin T.et.B.), Shorea laevis Ridl. (bangkirai), Cratoxylum arborescens BL. (geronggang), Alstonia angustifolia Hook.f. (pulai), dan Dacryodes rostrata H.J.L. (kemaisan). Pohon jenis Aquilaria malaccensis Lamk. (gaharu) telah masuk ke dalam status kelangkaan spesies dan kategori rawan menurut kriteria IUCN. Untuk itu spesies ini perlu dilestarikan.
Penampilan Padi Aromatik Varietas Gilirang di Sulawesi Selatan Ali Imran
Buletin Plasma Nutfah Vol 9, No 2 (2003): Desember
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumberdaya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/blpn.v9n2.2003.p7-9

Abstract

AbstractStudy on the performance of aromatic rice Gilirang variety was conducted at seven districts in South Sulawesi. Five thousand square meters of rice planted based on farmers cultural practices. The rice plant were transplanted at the planting distance of 25 x 25 cm with one plant per hill. In general plants were growing well. The yields were ranging from 4.8-8.1 t/ha with the average of 6.5 t/ha milling rice, which was higher than the check variety Ciliwung, 5.9 t/ha.AbstrakPenelitian dilakukan di lahan sawah irigasi di tujuh Kabupaten di Sulawesi Selatan pada bulan Mei - Oktober 2002. Penelitian menempati area seluas 5 are di tiap lokasi. Padi aromatik varietas Gilirang ditanam dengan jarak 25 x 25 cm, satu batang per rumpun. Hasil penelitian menunjukkan bahwa tanaman tumbuh baik dengan hasil berkisar antara 4,8-8,1 t/ha, rata-rata 6,5 t/ha GKG atau 9% lebih tinggi dari varietas nonaromatik Ciliwung.
Isolasi Bakteri Penghasil Enzim Protease Alkalin Termostabil Alina Akhdiya
Buletin Plasma Nutfah Vol 9, No 2 (2003): Desember
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumberdaya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/blpn.v9n2.2003.p38-44

Abstract

AbstractIn the effort of obtaining indigenous alkaline thermosteble protease producing bacteria, 17 different soil samples collected from slaughterhouses, fish ponds along the Nusa Tenggara, and spoiled milk disposal sites in Boyolali (Central Java) for microbial isolation. Out of the 1004 colonies obtained, 29 isolates showed proteolytic index ≥3.00. Enzymatic assay on these isolates resulted in two isolates (BYL-15 and BYL-28) with highest crude enzyme activity, this activity was also higher than that achieved by the standard bacteria (Bacillus firmus NRRL B-1107). After 36 hours of incubation, crude enzyme activity and specific activity of isolate BYL-15 were 106.45 U/ml filtrate and 5069.04 U/mg protein, respectively (at 50°C and pH 10.5). Whereas for isolate BYL-28 the crude enzyme activity and specific activity were 100.00 U/ml filtrate and 5,263.15 U/mg protein, respectively. The activity of these two isolates were about 10 times higher than that of the B. firmus (11.259 U/ml filtrate), whereas their specific activities were about 40 times than that of B. firmus (119.78 U/mg protein). Further studies showed that the proteolytic activity of these two isolates were still increased when assayed at 60°C, and more than one peak of activity were obtained when assayed at different pH values (8.5-12). Based on microscopic observations, these two isolates showed cell morphology characteristics of Actinomycetes. AbstrakDalam upaya menggali galur-galur bakteri penghasil enzim protease alkalin termostabil asli Indonesia, telah dilakukan isolasi mikroorganisme dari 17 contoh tanah yang diambil dari beberapa rumah pemotongan hewan dan tambak di Nusa Tenggara, serta tempat pembuangan susu rusak di Boyolali (Jawa Tengah). Dari 1004 isolat yang diperoleh, 29 di antaranya memiliki indeks proteolitik ≥3,00. Pengukuran terhadap aktivitas enzim isolat-isolat tersebut menghasilkan isolat BYL- 15 dan BYL-28 yang memiliki aktivitas enzim kasar yang lebih tinggi dibandingkan dengan bakteri pembanding (Bacillus firmus NRRL B-1107). Aktivitas enzim kasar dan aktivitas spesifik isolat BYL-15 setelah 36 jam berturut-turut adalah 106,45 U/ml filtrat biakan dan 5069,04 U/mg pada suhu 50°C dan pH 10,5. Aktivitas enzim kasar dan aktivitas spesifik isolat BYL-28 adalah 100,00 U/ml filtrat biakan dan 5263,15 U/mg pada kondisi yang sama. Nilai aktivitas enzim kedua isolat tersebut ±10 kali lebih besar daripada B. firmus (11,259 U/ml filtrat), sedangkan aktivitas spesifiknya 40 kali lebih besar daripada B. firmus (119,78 U/mg protein). Penelitian lebih lanjut menunjukkan bahwa aktivitas enzim kasar kedua isolat tersebut masih terlihat meningkat pada suhu 60°C dan memiliki lebih dari satu puncak aktivitas pada beberapa pH yang dicoba (8,5-12,0). Berdasarkan ciri-ciri morfologi yang diperoleh dari pengamatan mikroskopis, kedua isolat tersebut termasuk anggota Actinomycetes.
Pemanfaatan Teknik Kriopreservasi dalam Penyimpanan Plasma Nutfah Tanaman Ika Roostika; Ika Mariska
Buletin Plasma Nutfah Vol 9, No 2 (2003): Desember
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumberdaya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/blpn.v9n2.2003.p10-18

Abstract

AbstractThe two basic approaches for conservation of plant genetic resources are ex situ and in situ conservation. Cryopreservation is a potential method for long term preservation of plant germplasm. Cryopreservation technique could be used to preserve plant materials with unlimited time (until 20 years) because it bring the materials to metabolically inactive state and completely arrest the growth in liquid nitrogen at –196oC. This method is more efficient in terms of cost, time, space, and labour because it does not require frequenlty subculture. Cryopreservation techniques can be divided in to classical technique and new techniques. Classical technique can be applied to limited species but new techniques can be applied to wide range of species and many types of explant. The success of cryopreservation is not only indicated by the high rate of survival and regenerated culture, but also on the stability level of culture after cryopreservation.AbstrakPenyimpanan plasma nutfah dapat dilakukan secara ex situ dan in situ. Teknik kriopreservasi merupakan teknik penyimpanan jangka panjang. Dalam hal ini, bahan tanaman disimpan di dalam nitrogen cair yang mempunyai suhu -196oC. Pada suhu tersebut, bahan tanaman hampir sama sekali tidak mengalami proses metabolisme sehingga masa penyimpanan menjadi tidak terbatas (hingga 20 tahun). Penyimpanan dengan cara tersebut tidak memerlukan tindakan subkultur yang berulangulang sehingga lebih efisien dari segi biaya, waktu, ruang penyimpanan, dan tenaga. Teknik kriopreservasi dibedakan atas teknik lama dan teknik baru. Teknik lama hanya dapat diterapkan pada spesies tertentu, sedangkan teknik baru dapat diterapkan pada skala spesies yang lebih luas dan jenis eksplan yang lebih banyak. Keberhasilan teknik kriopreservasi tidak hanya ditunjukkan oleh kemampuan hidup dan regenerasi bahan tanaman pasca kriopreservasi tetapi juga ditentukan oleh tingkat stabilitas genetiknya.

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