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Biotropika
Published by Universitas Brawijaya
ISSN : 23027282     EISSN : 25498703     DOI : 10.21776/ub.biotropika.
Core Subject : Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology
Biotropika: Journal of Tropical Biology invites research articles, short communication, and reviews describing new findings/phenomena of biological sciences in tropical regions, specifically in the following subjects, but not limited to biotechnology, biodiversity, microbiology, botany, zoology, biosystematics, ecology, and environmental sciences.
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Articles 22 Documents
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Search results for , issue "Vol 2, No 1 (2014)" : 22 Documents clear
Perubahan Siklus Estrus Akibat Induksi Peningkatan Kadar Prostaglandin F2α (PGF2α) Pada Fase Luteal Kambing Peranakan Boer Wardhani, Aries Erlinda Ratna; W.M, Agung Pramana
Biotropika: Journal of Tropical Biology Vol 2, No 1 (2014)
Publisher : Biotropika: Journal of Tropical Biology

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ABSTRACT The objective of this study to understand the effect of PGF2α injection in early diestrus phase and mid- diestrus to the estrous cycle. Treatment in this study are early diestrus control, mid- diestrus control, administration of PGF2α on early diestrus phase and administration of PGF2α in mid diestrus phase (each group n=3.) 1.5 ml Capriglandin® was injected once intramusculary. Then, phase change observed every day start one day since injection. Estrous phase were determined by observation vaginal smear cytology. Data were analyzed by Mann- Whitney method using Microsoft Excel software and SPSS 16.0 for Windows. There’s no significant difference in diestrus and proestrus length on injected group at early diestrus and control early diestrus group, onset estrous of injected group and control group respectively on day 15 and on day 16. The injected group at mid diestrus and control mid diestrus group have a significant difference at onset estrous and diestrus length but there is no significant difference in proestrus length, onset of estrous in the injection group and control group respectively on day 6.7 and on day 12 day (P < 0.05). It can be concluded that there is no response of single injection of PGF2α when injected at early diestrus, but when injected at mid- diestrus causes a shorter diestrus phase than the control respectively 2,3 and 8 days. Keywords : PGF2α, onset of estrous, estrous synchronization, vaginal smears
Efek Refugia pada Populasi Herbivora di Sawah Padi Merah Organik Desa Sengguruh, Kepanjen, Malang Ria Pravita Sari; Bagyo Yanuwiadi
Biotropika: Journal of Tropical Biology Vol 2, No 1 (2014)
Publisher : University of Brawijaya

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ABSTRAK Hama tanaman mengakibatkan hasil produksi padi di Indonesia menurun. Untuk itu diperlukan suatu upaya pengendalian hama tanpa merusak kestabilan lingkungan yaitu dengan memanfaatkan blok refugia. Tujuan dari penelitian ini yaitu untuk mengetahui kelimpahan, diversitas, famili yang mendominasi pada lahan sawah dan blok refugia di setiap fase pertumbuhan padi serta untuk mengetahui efek blok refugia  terhadap hebivora di sawah Kepanjen. Metode koleksi data tentang kunjungan herbivora yang dipakai dalam penelitian ini adalah visual control. Pada pelaksanaannya, pengamatan herbivora dilakukan pada 5 plot yang dibuat di sawah  dan 1 plot blok refugia di pematang sawah tersebut. Jarak pengamat dari plot sawah maupun blok refugia adalah 2 meter dan pengamatan tentang kunjungan herbivora dilakukan selama 15 menit untuk setiap plotnya. Pengamatan dilakukan 3 periode (pukul 08.00-10.00, 12.00-14.00, dan 15.00-17.00) untuk setiap fase pertumbuhan padi dan masing-masing diulang 2 kali. Pengukuran faktor abiotik meliputi suhu, kelembaban udara dan intensitas cahaya. Nilai penting dan indeks diversitas (Shannon-Wienner) dihitung untuk mengetahui analisis struktur komunitas herbivora. Hasil penelitian menunjukkan bahwa kelimpahan herbivora keseluruhan berjumlah 521 individu yang terdiri dari 5 ordo dengan 13 famili. Famili yang memiliki kelimpahan tertinggi di sawah maupun blok refugia yaitu Acrididae. Diversitas herbivora di sawah maupun blok refugia tergolong rendah hingga sedang. Diversitas herbivora tertinggi di sawah yaitu pada pukul 12.00-14.00 WIB sedangkan di blok refugia diversitas tertinggi terjadi pada pukul 08.00-10.00 WIB. Famili yang mendominasi di sawah maupun blok refugia yaitu Acrididae ditandai dengan tingginya INP.   Kata kunci: Herbivora, organik, pengendalian hayati, refugia   ABSTRACT Pests of plants causes  decreasing of rice production in Indonesia. That’s why it required an effort to control pests without harming the environment stability by using refugia. The objectives of this research were to know the abundance, diversity and family that dominate in the crops and in the refugia block in every stage of rice growth and to determine the effects of refugia block to the herbivore population in Kepanjen rice field. The method used to collect the information of herbivore visited both padi plots and refugia plots in this research was visual control. For those purposes, visual control were conducted on 5 plots in rice field and 1 plot in refugia plots. The distance of the observer to each plot was 2 meters and it were conducted for 15 minutes for each plot. Those observations were made in 3 period (08.00 to 10.00, 12.00 to 14.00, and 15.00 to 17.00) for every stage of rice growth and it were repeated 2 times. The measurement of abiotic factors include temperature, air relative humidity and light intensity. The analysis of herbivore community structure was obtained by counting the important value and Shannon–Wienner diversity index). Total abundance of herbivores was 521 individuals consist of 5 orders with 13 families. Families which had the highest abundance in the fields and refugia was Acrididae. Herbivore diversity in the fields and refugia was low to moderate. The highest of herbivore diversity in rice fields was at 12.00 to 14.00 pm while the highest diversity in refugia was at 8.00 to 10.00 pm. Families that dominate in the fields and refugia was Acrididae with high INP value.   Keywords : Biological control,  herbivore, organic, refugia
Promosi Pelestarian Suweg dan Pemanfaatan Kembali Bekatul Padi Organik Melalui Olahan Mie Hitam pada Beberapa Ibu Guru Kota Malang Purfita Asmaranti; Endang Arisoesilaningsih
Biotropika: Journal of Tropical Biology Vol 2, No 1 (2014)
Publisher : University of Brawijaya

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Suweg (Amorphophallus campanulatus Blume) dan bekatul mulai jarang dikenal masyarakat. Keterbatasan pengolahan, menjadi faktor utama rendahnya minat pelestarian umbi suweg dan pemanfaatan bekatul oleh masyarakat. Penelitian ini bertujuan untuk meningkatkan pemahaman masyarakat terutama ibu-ibu dalam pendidikan konservasi umbi suweg dan pemanfaatan bekatul melalui kegiatan promosi. Evaluasi keberhasilan promosi ditentukan dengan teknik wawancara menggunakan lembar kuisioner kepada 10 ibu guru dari masing-masing SD tengah dan SD pinggiran Kota Malang. Alat (tool)  promosi yang digunakan meliputi mie hitam non terigu dari umbi suweg dan bekatul, serta bibit suweg. Data yang diperoleh dianalisis dengan uji Wilcoxon dengan membandingkan pemahaman ibu-ibu sebelum dan sesudah promosi. Hasil analisis menunjukkan bahwa promosi yang dilakukan dengan membagikan mie hitam dan bibit suweg dapat  meningkatkan pemahaman ibu-ibu tentang suweg dan bekatul. Demikian pula, kesediaan ibu-ibu untuk menanam suweg meningkat 20% sesudah promosi.  Selain itu, mie hitam potensial sebagai makanan fungsional serta efisien sebagai alat promosi pelestarian suweg dan pemanfaatan bekatul.
Diversitas Arthropoda Tanah di Lahan Kebakaran dan Lahan Transisi Kebakaran Jalan HM 36 Taman Nasional Baluran Mustofa Halli; I Dewa Agung; Bagyo Yanuwiadi
Biotropika: Journal of Tropical Biology Vol 2, No 1 (2014)
Publisher : University of Brawijaya

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Aim of this experiment to determine arthropods diversity at HM36 Baluran National park after burned and to know it relation with human activity. This experiment held in November 2013. Taking sample using pit fall trap methods at burned land and transition land after burned with two sampling spot and three pit fall jam for each spot. Sample identified with morphological analyze and abundance measurement, Frequency, relative abundance, frequency abundance, and  importance value rank. Diversity analyze using Shannon-Wiener index and Bray Curtis Index to determine similarity level for each location. After burned land has 2.05 and 1.04 at transistion land after burned from diversity analyze. Formicidae dominating at every location of sampling. Each location is different each other with only 25% of Bray Curtis analyze measurement. Abiotic and Colony domination effecting diversity level and environment condition.   Keyword : Pit fall trap, Importance value rank, Shannon wiener index, Bray Curtis index
BRADYKININ B2 RECEPTOR GENE POLYMORPHISM ANALYSIS IN HYPERTENSIVE PATIENTS AT DR SAIFUL ANWAR HOSPITAL MALANG Johan Aloysius; Nashi Widodo
Biotropika: Journal of Tropical Biology Vol 2, No 1 (2014)
Publisher : University of Brawijaya

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Hypertension is a major health problem in the world. When blood volume is low, juxtaglomerular cells in the kidneys secrete renin and prorenin activates the blood circulation. Plasma renin converts angiotensinogen released by the liver into angiotensin I, which is then converted to angiotensin II by the enzyme angiotensin converting enzyme (ACE) located in the pulmonary vascular endothelium. Angiotensin II causes blood vessels to constrict, resulting in increased blood pressure. ACEi inhibit the formation of angiotensin I into angiotensin II, and activate Bradykinin (BK), which causes blood vessels to dilate, thereby reducing blood pressure. However, administration of ACEi periodically cause the accumulation of BK in pulmonary vessels that cause inflammation (cough) in some people who suspected mediated by Bradykinin B2 receptor (BK2R) gene. Total of the subjects in this study is 52 subjects, DNA extracted from white blood cells (leukocytes) as much as 200 mL from whole blood by using the QIAamp kit (Qiagen). Gene BK2R in humans amplified by the method of Polymerase Chain Reaction (PCR) method using the following primers F (5-GCAGAGCTCAGCTGGAGGCG-3), and R (5-CCTCCTCGGAGCCCAGAAG-3) and identified by the method of Single Strand Conformation Polymorphism (SSCP). Results of this study shows three different genotypes there are CC, CT, and TT. Subjects with the CC genotype were most susceptible to cough, and subjects with CT genotype were least susceptible.   Keywords : ACE inhibitors, B2 Receptor, Cough, SSCP
INTERAKSI ANTARA CAPUNG DENGAN ARTHROPODA DAN VERTEBRATA PREDATOR DI KEPANJEN, KABUPATEN MALANG Bernadeta Putri Irma Dalia; Amin Setyo Leksono
Biotropika: Journal of Tropical Biology Vol 2, No 1 (2014)
Publisher : University of Brawijaya

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Penelitian ini bertujuan untuk mengetahui interaksi antara capung dengan Arthropoda dan vertebrata di lahan pertanian Kepanjen, Jawa Timur. Pengamatan lapang dilakukan pada bulan Juni sampai Agustus 2013 pada area pertanian padi di Desa Sengguruh, Kecamatan Kepanjen, Kabupaten Malang, Jawa Timur. Metode yang digunakan adalah visual kontrol, kemudian analisis data dilakukan secara deskriptif. Capung, Athropoda, dan vertebrata yang ditemukan selama pengamatan memiliki interaksi predasi. Capung berperan sebagai predator berbagai Arthropoda di pertanian, terutama bagi ordo Lepidoptera, Hymenoptera, Hemiptera, Orthoptera, dan Diptera. Capung juga menjadi mangsa bagi Arthropoda, seperti Araneida, dan vertebrata, seperti Anura,  Mabuya sp., dan Todiramphus chloris. Kata kunci : Capung, pertanian
KUALITAS SEMEN SEGAR KAMBING BOER PADA TEMPERATUR PENYIMPANAN 4 OC DENGAN MENGGUNAKAN PENGENCER SITRAT DAN SUPLEMENTASI SUSU KEDELAI BUBUK Winda Rahayu; Agung Pramana W.M
Biotropika: Journal of Tropical Biology Vol 2, No 1 (2014)
Publisher : University of Brawijaya

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ABSTRACT The objective of this research was to know the influence of soybean milk powder diluents based on concentration (2%, 4%,6%) in citrate diluent to motility, viability, abnormality and membrane integrity of spermatozoa based on long duration storage (0 hours, 24 hours, 72 hours, 120 hours). This research using diluent medium based on vegetable medium (lechitin) is andromed (K+), soybean milk in diluent citrate with concentrations of 0% (K-), 2% (F1), 4% (F2), and 6% (F3). The characterization of predilution were observed such as volume, color, consistency, pH, mass motility, individual motility, viability, concentration, and abnormality. Next step dilution based on treatment with each of the three  recurrence and performed storage at temperature of 4˚C. The next of post storage were observed such as sperm viability, motility, abnormality and membrane integrity. Data were analyzed using two way ANOVA in factorial completely randomized design with SPSS version 16.0 and continued with Tukey test. The results showed that a significant difference (P<0.05)  to K+, F1, and F2 diluent to K- and F3 diluent. The presence of lechitin content of soybean milk can protect spermatozoa from cold shock during storage at a temperature of 4˚C. The concentration of soy milk powder high possibility changes to pH of the media and influenced of osmotic presure on sperm membrane decrease of the sperm quality during storage. Key word: Lechitin, plasma membrane, soybean milk
Local People Knowledge about Plant Trees Species Inside Tropical Forest of Tengger Ranupani Setyo Ayu Hardyanti; Luchman Hakim
Biotropika: Journal of Tropical Biology Vol 2, No 1 (2014)
Publisher : University of Brawijaya

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Degradation of  tropical forests in Indonesia is  known more widespread. The development of  societyactivities  is  known to be very fast, thus  it  causes  land degradation. An  effort  to make ecosystem reparation of the tropical forest through restoration becomes very important to be done. The success of these efforts cannot be separated from the local community factors. The purpose of this study  is  to determine rainforest woody plant species that are recognized, and utilized  also knowing the  public perceptions in tropical forest restoration. The method used is quantitative ethnobotany with estimatingthe significance of the plant based on the level of popularity  herbs known and frequently used by the society.  The data  were analyzed using the Relative Frequency of Citations (RFC) and Relative Importance (RI). There are thirtyfive plants that are mentioned by society. Eighteen species are woody plants. The highest value of RFC from the interview are pine (Casuarina junghuhniana) that the value is  1. It  is often  used to  fill the need  as a building material and firewood.  The  smallest value of  RFC is 0.07  randu bacin  (Pittosporum moluccanum).  It  is  caused of lacking knowledge of  the type  and the benefits of the plants. RI value or numbers of plants  that are most frequently mentioned is fir that is at 3.56.  Meanwhile,  the  smallest  value of  RI  is  randu bacin  (Pittosporum moluccanum)  of 0.31.  The society’s perception of woody plants is  quite well. The society  knows the benefits of forest plants and the  forest condition today.  The society’s  traditional knowledge in  benefitting  the  plants  is  to be hereditary. So that  society  makes  serious efforts  to  help  the activity of  forest restoration by planting vegetation. Keywords: degradation, forest, restoration, trees, utilization
Etnobotani Penyambutan Bulan Sura Di Komplek Wisata Alam Air Terjun Sedudo, Kabupaten Nganjuk Prita Ayuningtyas; Luchman Hakim
Biotropika: Journal of Tropical Biology Vol 2, No 1 (2014)
Publisher : University of Brawijaya

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Abstrak Air Terjun Sedudo merupakan salah satu daerah wisata yang memiliki atraksi budaya dengan memanfaatkan tumbuhan sebagai pelengkap acara. Tujuan penelitian adalah untuk mengetahui jenis tumbuhan yang digunakan dalam kegiatan menyambut Bulan Sura oleh masyarakat sekitar Air Terjun Sedudo sebagai bagian dari strategi konservasi tumbuhan. Penelitian dilaksanakan pada bulan September 2013 hingga April 2014. Observasi secara langsung dilakukan di komplek wisata alam Air Terjun Sedudo. Identifikasi tumbuhan dan analisis data dilakukan di Laboratorium Taksonomi, Struktur dan Perkembangan Tumbuhan Jurusan Biologi FMIPA Universitas Brawijaya. Penentuan responden dengan teknik purposive sampling. Data didapatkan menggunakan metode wawancara secara semi terstruktur. Data hasil wawancara pemanfaatan tumbuhan diklasifikasikan dalam 8 kategori pemanfaatan, dan selanjutnya dianalisis menggunakan indeks etnobotani. Hasil penelitian menunjukkan didapatkan 62 spesies tumbuhan dan tergolong ke dalam 34 famili yang dimanfaatkan untuk pelengkap prosesi penyambutan Bulan Sura. Tumbuhan yang memiliki RFC tertinggi adalah ketela pohon (Manihot esculenta Crantz) sebesar 0,77 dan untuk nilai RI tertinggi adalah melati (Jasminum sambac L.) sebesar 0,91. Sebagian besar tumbuhan yang dimanfaatkan digunakan untuk melengkapi prosesi penyambutan Bulan Sura maupun untuk memenuhi kebutuhan hidup sehari-hari. Masyarakat sekitar Air Terjun Sedudo sebagian besar masih bergantung dengan sumberdaya dan tumbuhan yang ada di sekitar tempat tinggalnya, sehingga secara tidak langsung masyarakat melakukan konservasi guna melestarikan sumberdaya yang ada.   Kata kunci : etnobotani, ekowisata, konservasi tumbuhan, konservasi atraksi wisata
Perubahan Siklus Estrus Akibat Induksi Peningkatan Kadar Prostaglandin F2α (PGF2α) Pada Fase Luteal Kambing Peranakan Boer Aries Erlinda Ratna Wardhani; Agung Pramana W.M
Biotropika: Journal of Tropical Biology Vol 2, No 1 (2014)
Publisher : University of Brawijaya

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ABSTRACT The objective of this study to understand the effect of PGF2α injection in early diestrus phase and mid- diestrus to the estrous cycle. Treatment in this study are early diestrus control, mid- diestrus control, administration of PGF2α on early diestrus phase and administration of PGF2α in mid diestrus phase (each group n=3.) 1.5 ml Capriglandin® was injected once intramusculary. Then, phase change observed every day start one day since injection. Estrous phase were determined by observation vaginal smear cytology. Data were analyzed by Mann- Whitney method using Microsoft Excel software and SPSS 16.0 for Windows. There’s no significant difference in diestrus and proestrus length on injected group at early diestrus and control early diestrus group, onset estrous of injected group and control group respectively on day 15 and on day 16. The injected group at mid diestrus and control mid diestrus group have a significant difference at onset estrous and diestrus length but there is no significant difference in proestrus length, onset of estrous in the injection group and control group respectively on day 6.7 and on day 12 day (P < 0.05). It can be concluded that there is no response of single injection of PGF2α when injected at early diestrus, but when injected at mid- diestrus causes a shorter diestrus phase than the control respectively 2,3 and 8 days. Keywords : PGF2α, onset of estrous, estrous synchronization, vaginal smears

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