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Dinia R Dwijayanti,
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Biotropika
Published by Universitas Brawijaya
ISSN : 23027282     EISSN : 25498703     DOI : 10.21776/ub.biotropika.
Core Subject : Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology
Biotropika: Journal of Tropical Biology invites research articles, short communication, and reviews describing new findings/phenomena of biological sciences in tropical regions, specifically in the following subjects, but not limited to biotechnology, biodiversity, microbiology, botany, zoology, biosystematics, ecology, and environmental sciences.
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Articles 22 Documents
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Search results for , issue "Vol 3, No 2 (2015)" : 22 Documents clear
Growth of Pioneer Grasses in Monoculture and Polyculture Systems of Hydroseeding Applied in a Coal Mining Tailing from the South Kalimantan Amalia Fadhila Rahma; Endang Arisoesilaningsih
Biotropika: Journal of Tropical Biology Vol 3, No 2 (2015)
Publisher : University of Brawijaya

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Abstract

ABSTRACT   The aims of this research were to compare germination of local and pioneer grasses seeds sown in garden soil and hydroseeding mulch. Furthermore, we also observed growth, density and coverage of the grasses planted in monoculture or polyculture systems applied in a post coal mining tailing from the South Kalimantan. Species used were Eleusine indica (L.), Paspalum conjugatum P.J.Bergius, Sporobolus indicus (L.) R.Br. and Eulalia amaura (Buese) Ohwi. Thirty two seeds of each grass were sown on a garden soil and mulch of hydroseeding to observe rate and time of germination. The seds of each species (in monoculture treatment) and composited species (in polyculture treeatment) were then mixed with mulch and spreaded above the coal mining tailing put in plastic pot. Each treatment were replicated 3 to 5 times. The observed variables were the time and rate of germination, density, maximum length of leave blade, plant height and coverage, root/shoot length ratio. Data were analyzed descriptively and statistically using one way Anova, Brown Forsythe or t tests, cluster and biplot analysis. Results of research showed that seeds of all pioneer grasses sown in monoculture germinated in both media, garden soil and hydroseeding mulch. Otherwise, E. indica and E. amaura seeds were less germinated in polyculture system. In monoculture system, germination rate of seeds sown on the  soil was higher, moreover seeds rapidly germinated rather than those of hydroseeding mulch. Density, growth and coverage of monoculture grass tent higher than polyculture ones. Root system of all species developed well in the mining tailing, therefore its grew longer than their shoot. Key word : Coal mining tailing, germination, hydroseeding, pioneer grasses
Diversitas dan Uji Potensi Bakteri Kitinolitik dari Limbah Udang Imanda Nurul Setia; Suharjono Suharjono
Biotropika: Journal of Tropical Biology Vol 3, No 2 (2015)
Publisher : University of Brawijaya

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Abstract

ABSTRAK Proses pengolahan udang dari industri akan menghasilkan limbah cair dan padat sebanyak 30 – 75 % dari berat udang. Limbah udang mengandung 20 – 60 % kitin sehingga dapat menjadi sumber bakteri kitinolitik. Tujuan penelitian ini adalah untuk mengetahui diversitas bakteri kitinolitik dan mengetahui potensi bakteri yang memiliki aktivitas kitinolitik yang tinggi pada limbah udang. Tahapan penelitian meliputi isolasi bakteri pada media CCA (Colloidal Chitin Agar), penghitungan diversitas bakteri kitinolitik, dan seleksi isolat berdasarkan indeks kitinolitik. Diversitas bakteri kitinolitik dihitung menggunakan indeks diversitas Simpson. Penelitian uji potensi kitinolitik menggunakan Rancangan Acak Lengkap (RAL) dengan tiga kali ulangan. Data indeks kitinolitik dianalisis menggunakan analisis ragam dilanjutkan uji Games-Howell (α = 0,05). Indeks diversitas limbah padat lebih tinggi (0,646) daripada limbah cair (0,213). Dua dari 18 isolat yaitu isolat PBK 2 (berasal dari limbah cair) dan SA 1.2 (berasal dari limbah padat) memiliki indeks kitinolitik tertinggi yaitu secara berturut-turut sebesar 2,069 dan 2,084. Kata kunci : bakteri kitinolitik, limbah cair, limbah padat, kitin, udang ABSTRACT Shrimp processing from industrial activity produce solid waste and wastewater 30 – 75 % from shrimp weight. Shrimp waste contains 20 – 60 % chitin and possible to be source of chitinolytic bacteria. The objectives of this research was to observe diversity of chitinolytic bacteria and to analyze potency of bacteria which have high activity of chitin degradation in shrimp waste. The research consist of isolation of bacteria using Colloidal Chitin Agar (CCA) medium, quantification of chitinolytic bacteria diversity, and screening of bacteria based on chitinolytic index. Diversity of chitinolytic bacteria quantified with Simpson diversity index. Chitinase assay carried out according Complete Randomized Design with three replications. Diameter of clear zone data was analyzed using analysis of variance continued with Games-Howel test (α = 0.05). Diversity index of solid waste more higher (0.646) than wastewater (0.213). Only two of eighteen isolates of chitinolytic bacteria (PBK 2 and SA 1.2 isolates) showed highest chitinolytic index. Key words: chitinolytic bacteria, chitin, shrimp, waste

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