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Edy Kurnianto
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Journal of the Indonesian Tropical Animal Agriculture
Published by Universitas Diponegoro
ISSN : 20878273     EISSN : 24606278     DOI : -
Journal of the Indonesian Tropical Animal Agriculture (JITAA) is a double blind peer-reviewed publication devoted to disseminate all information contributing to the understanding and development of animal agriculture in the tropics by publication of original research papers
Articles 8 Documents
Search results for , issue "Vol 48, No 4 (2023): December" : 8 Documents clear
SNPs in splicing region and miRNA binding region of Bos taurus TREM-1 gene reveals its association with mastitis N. S. Pandupuspitasari; F. A. Khan; A. Setiaji; D. A. Lestari; Z. Shujun
Journal of the Indonesian Tropical Animal Agriculture Vol 48, No 4 (2023): December
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.48.4.306-314

Abstract

Proper splicing is important for the functioning of a gene, and any interruption in splicing causes several deleterious events. Triggering receptors present on myeloid cells, TREM-1, are implicated in inflammation and act as amplifiers by mediating the release of proinflammatory chemokines and cytokines in response to fungal and bacterial infections. In bovines, mastitis is an inflammatory disease in which mammary gland inflammation is generally caused by bacteria. We found rs109937179 and rs208224995 SNPs in the splicing and miRNA binding region of TREM-1 gene in Chinese Holstein cows. The genotype distribution of the alleles for TREM-1 (rs109937179 and rs208224995) gene polymorphisms was investigated in 364 and 320 Chinese Holstein cows, respectively. We found that the GG genotype of the rs109937179 polymorphism and rs208224995 genotype of CA within the TREM-1 gene were associated with an increased risk of mastitis. Importantly, rs109937179 was found in the splicing region of TREM-1, and rs208224995 has a miRNA binding region for bta-miR- 2329-3p in the 3'UTR, which determines its effective roles in gene expression regulation.
Exploring the potential of natural feed additives from herbs as an alternative to antibiotic growth promoters for Mojosari layer duck (Anas javanica) farming: in-silico and in-vivo studies I. H. Djunaidi; C. A. Damayanti; S. Wibowo; O. Sjofjan
Journal of the Indonesian Tropical Animal Agriculture Vol 48, No 4 (2023): December
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.48.4.243-257

Abstract

Using synthetic feed additive, such as antibiotic growth promoters (AGPs), is common in Mojosari layer duck (Anas javanica) farms. Their purpose is to enhance growth and productivity. However, the indiscriminate use of AGPs has been associated with the emergence of bacterial resistance. This leads to lingering effects and potential health hazards for poultry and consumers. In light of these concerns, natural herb feed additives have emerged as an alternative strategy to AGPs in poultry feed. The active compounds in natural feed additives can improve feed efficiency and enhance production performance, resulting in higher-quality poultry products, especially eggs. However, it is known that the active compounds in phytobiotics derived from herbs contain anti-nutrients that can affect productivity and alpha-amylase in poultry. This study was aimed to investigate the interconnection between active compounds in phytobiotics and alpha-amylase using in-silico methods and verify the findings with field data using in-vivo methods. Alpha-Amylase is a vital enzyme that catalyzes the hydrolysis of the glycosidic bonds present in glycogen, resulting in the formation of maltose, a water-soluble disaccharide. Based on the results of docking involving nine phytobiotic compounds and their interaction with glycogen, it was observed that andrographidine E showed a binding affinity of 8.8 kcal/mol. Furthermore, these compounds form conventional hydrogen bonds, facilitated the formation of stable bonds between proteins and compounds. In particular, the interacting amino acids did not exhibit unfavourable bonds. Therefore, andrographidine E was more stable compared to other compounds. Nonetheless, the results show that several compounds have weaker binding affinity than the enzyme's binding to glycogen. However, the hydrophobic binding types of these compounds did not seem to impact the performance of the alpha-amylase enzyme, as demonstrated by in vivo data.
Moringa oleifera leaf for replacing protein portion of soybean meal in the diet of young growing meat goats J. Achmadi; E. Pangestu; S. Surahmanto; A. Muktiani; S. Sutrisno; M. Christiyanto; S. Surono; L. K. Nuswantara; A. Subrata
Journal of the Indonesian Tropical Animal Agriculture Vol 48, No 4 (2023): December
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.48.4.315-321

Abstract

In vitro ruminal feed fermentability test and feeding experiment were conducted to clarify the substitution of soybean meal with Moringa oleifera leaf powder (MOlp) in the diet of young growing goats. Five M. oleifera based feeds were studied in the test of in vitro ruminal fermentability: 0% MOlp, 25% MOlp, 50% MOlp, 75% MOlp and 100% MOlp. All feeds were designed to be isoprotein and isoenergy, containing 20% crude protein and 71 - 75% TDN. In the feeding experiment, three dietary experiments were offered to young growing Jawarandhu goats, aged 3 – 5 months with average body weight of 10 kg. The dietary experiments were 0% MOlp, 25% MOlp and 75% MOlp. All dietary experiments were designed to be isoprotein and isoenergy, containing 20% crude protein and 71 - 75% TDN. Result of the in vitro ruminal feed fermentability test showed that feed dry matter and crude protein digestibilities, ruminal concentrations of total VFA and NH3, and total ruminal protein production increased (p<0.05) in line with the level of MOlp in the experimental feeds. Result of feeding experiment revealed that nutrient intakes and body weight gain were not different (p>0.05) among treatment groups. Blood concentrations of protein, urea, ammonia, glucose, triglycerides, and cholesterol were similar in all groups. The dietary treatment did not effect significantly (p>0.05) on some hematological parameters. M. oleifera leaf could replace protein portion of soybean meal in the diet of young growing meat goats without negative effect on productive performances.
Grounding the economic selection index for evaluation and selection of dairy cattle S. Ruban; V. Danshyn; M. Matvieiev; I. Lastovska; O. O. Borshch; O. V. Borshch; V. Bilkevych; M. Fedorchenko; V. Lykhach
Journal of the Indonesian Tropical Animal Agriculture Vol 48, No 4 (2023): December
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.48.4.258-268

Abstract

The aim of the research was to develop a selection index for the evaluation and selection of dairy cattle for increasing milk production and reproductive traits. Materials of the research were data on milk production and reproduction of cows of the Ukrainian Black-and-White dairy breed in commercial farm in Kharkiv region. Studies showed that there was a certain antagonism between milk productivity and reproduction of the Ukrainian Black-and-White dairy cow. The coefficients of phenotypic correlation between milk yield, fat and protein yield and the days open ranged from +0.284 to +0.293. Selection indexes were built, which included protein, fat yield, and days open. The developed selection indexes were characterized by a high relationship with the protein yield (α<0.001), much lower – with the fat yield (α<0.05), while the relationship of the selection indexes with the days open was almost absent. The calculated estimates of correlation and regression coefficients made possible to predict correlated responses of milk production and reproduction traits on selection index. The results showed that the developed selection indexes allow increase the milk production of cows and at the same time to prevent decrease in the level of reproduction.
Addition of soybean meal extract with Lactobacillus plantarum in rations on protein digestibility and performance of broiler chickens R. Afro&#039;; V. D. Y. B. Ismadi; L. Krismiyanto; M. Mulyono
Journal of the Indonesian Tropical Animal Agriculture Vol 48, No 4 (2023): December
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.48.4.322-336

Abstract

This study examined the effect of adding soybean meal extract (SME) and Lactobacillus plantarum (LP) on protein digestibility and performance of broiler chickens. The material used was 8-day-old Cobb CP 707 strain broilers with a body weight of 137.89 ± 3.7 g. This study used a completely randomized design with 6 treatments and 4 replicates, with each replicate of 8 birds. The treatments were T0: basal diet (control), T1: basal diet + LP 1.2%, T2: basal diet + SME 0.15%, T3: basal diet + SME 0.30%, T4: basal diet + SME 0.15% + LP 1.2%, T5: basal ration + SME 0.30% + LP 1.2%. Parameters measured were the performance of broiler chickens, lactic acid bacteria population, Escherichia coli population, intestinal pH, protein consumption, protein digestibility, and antioxidant activity. The results showed that the addition of SME and Lactobacillus plantarum in the ration had a significant effect (P <0.05) on total daily weight gain, total daily feed intake, total feed conversion, lactic acid bacteria population, Escherichia coli population, intestinal pH, protein consumption, protein digestibility, and antioxidant activity. Significantly higher total daily weight gains were observed in T5 (54.09 g) compared to T3 (51.27 g), T2 (46.98 g), T1 (46.64 g), and T0 (45.56 g). Total daily feed intake of T5 (79.94 g) was significantly higher those of T2 (74.52 g), T1 (74.17 g), and T0 (74.89 g). Feed conversion ratio was significantly lower in T5 (1.48) compared to the others, but not different from T4 (1.54). The LAB population of T5 (10.26 log cfu/g) was significantly higher than those of T2 (8.98 log cfu/g), T1 (8.99 log cfu/g), and T0 (6.99 log cfu/g). The Escherichia coli population of T5 (1.50 log cfu/g) was significantly lower than T1 (2.40 log cfu/g) and T0 (4.54 log cfu/g). Intestinal pH of T5 (5.90) was significantly lower than the others, but not different from T4 (5.91). Protein consumption of T5 (17.14 g) was significantly higher than T2 (15.98 g), T1 (15.90 g), and T0 (16.00 g), and protein digestibility of T5 (88.53%) was significantly higher than T2 (85.42%), T1 (82.92%), and T0 (80.37%). Malondialdehyde of T5 (0.80 nmol/ml) was significantly lower than T2 (1.01 nmol/ml), T1 (1.03 nmol/ml), and T0 (1.27 nmol/ml). Superoxide dismutase of T5 (21.43 U/ml) was significantly higher than all treatments. The study concludes that adding soybean meal extract with 0.30% and Lactobacillus plantarum 1,2% (T5) was effective in increasing protein digestibility and optimizing performance in broiler chickens.
Histopathological study and molecular characterization of Infectious Bronchitis virus circulating in broilers in Basrah governorate, Iraq N. H. Megdad; W. M. Seger; Y. J. Mohammed
Journal of the Indonesian Tropical Animal Agriculture Vol 48, No 4 (2023): December
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.48.4.269-281

Abstract

The poultry industry plays an important role in the food economics and the food industry in both their layer and broiler chicken. Poultry diseases on the other way impact in the poultry industry especially diseases from virus sources since it does not have a specific treatment except for vaccines with routine prevention and control. This study was designed for the assessment of histopathological changes, molecular diagnosis and phylogenetic analyses of the Infectious Bronchitis virus circulating in broilers in Basrah province in the south of Iraq. The tissue samples were (trachea, lung and kidney). One hundred samples were studied with clinical signs and lesions related to IB. The histopathological changes caused by the virus were studied with hematoxylin and eosin stain, and the causative agent Infectious Bronchitis Virus (IBV) was identified using a polymerase chain reaction combined with explaining the phylogenetic analysis. The results of molecular diagnosis and identification showed the twelve Iraqi field strains of IBV were closely related and shared a high identity of 98.44 %- 99.69 % of each other. Variant 2(IS/1494 like) was a predominant genotype of IBV in the present study, and has high similarity with the variant 2(IS/1494) like a strain of IBV circulating in Iran and in the Kurdistan region north of Iraq. The histopathological findings were desquamation of the cilia of the mucosa layer of the trachea, infiltration of inflammatory cells, hyperplasia of goblet cells with thickening of mucosal layer in some areas infiltration of inflammatory exudate within capillaries and Para bronchus, where the microscopic results in kidney were showed interstitial hemorrhage, necrosis of the renal tubular epithelium, infiltration of inflammatory cell. The present results provided useful information on target organs damaged by IBV and the genetic epidemiology of such virus strains circulating in Basrah governorate Iraq during this year that would be helpful to develop a local vaccine.
The biological role of clove oil in the diet of mature male rabbits on the physiological body functions, oxidative stress and physical semen properties A. A. M. Habeeb; M. A. A. Atta; A. I. A. Elhanafy; A. K. Sharaf
Journal of the Indonesian Tropical Animal Agriculture Vol 48, No 4 (2023): December
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.48.4.282-296

Abstract

In an eight-week experiment conducted throughout the months of January and February, 2023, the goal of this study was to determine how changing the diet of mature male rabbits to include clove oil affected their body's physiological processes and the quality of their sperm. In this study, 24 mature rabbit bucks that were 8 months old and weighed an average of 2.650 g in the first production year were used. In a final randomized plan, the bucks were randomly assigned to two experimental groups, each with twelve rabbits. Bucks served as the control group in the first group and were fed a commercial pelleted food without any supplements. Each buck in the second group received one milliliter of clove along with the daily diet requirement from commercial pellets. Every two weeks, values for weight gain, feed consumption, testosterone levels, blood constituents, libido, and semen quality were assessed. The findings demonstrated that including clove oil in the diet of bucks rabbits resulted in a significant (P<0.01) improvement in each of daily feed intake, daily weight gain, testosterone hormone, and γ-globulin and a significantly (P<0.01) reduced glucose, cholesterol fraction levels in comparison to the control group. Additionally, adding clove oil increased (P<0.01) the rabbit bucks' physical semen attributes values. It was determined that including clove oil in the diet of male rabbits improved physiological body processes, produced hypercholesterolemia, and raised the quality of the rabbits' sperm without having a negative impact on liver or kidney function.
The improvement of meat nutrient composition in broiler chickens fed diets containing rice bran tempeh supplemented with cellulose U. Santoso; B. Brata; K. Kususiyah; M. Dani
Journal of the Indonesian Tropical Animal Agriculture Vol 48, No 4 (2023): December
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.48.4.297-305

Abstract

This study was aimed to evaluate the effect of cellulase supplementation in diets containing rice bran tempeh on body conformation, fat deposition, nutritional composition of broiler meat and blood biochemical concentrations in broiler chickens. This research was carried out for 3 weeks from 15 days to 35 days of age. Two hundred broilers aged 15 days were divided into 4 treatment groups with 5 replications (8 broilers for each replication) for each treatment, as follows: T1 = 0% cellulase; T2 = 0.05% cellulase; T3 = 0.1% cellulase, and T4 = 0.15% cellulase. Body conformation, chemical meat composition, fat deposition, blood pH, glucose and uric acid were measured. The addition of 0.05% or 0.15% cellulase significantly reduced leggedness (P<0.05), whereas 0,1% or 0,15% cellulase addtion significantly reduced the content of meat fat and ash plus carbohydrate. In addition, the inclusion of 0.05% cellulase increased the content of meat protein (P<0.01). The inclusion of cellulase from 0.05-0.15% did not decrease fat deposition, blood concentration of uric acid, cholesterol, glucose and pH. In conclusion, the addition of 0.1% or 0.15% cellulase improved meat nutrient composition without improving body conformation.

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