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Articles 15 Documents

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Search results for , issue "Vol 1, No 3 (2012)" : 15 Documents clear

Antagonist Mold Exploration of Phytophthora spp. Pathogen on Apple Plant Nugrahani, Meisarina; Suharjono, Suharjono; Endarto, Otto
Natural B, Journal of Health and Environmental Sciences Vol 1, No 3 (2012)
Publisher : Natural B, Journal of Health and Environmental Sciences

The attack of Phytophthora spp. caused the descrease of apple crops production up to 90 %. Some antagonist mold species are able to inhibit the growth of Phytophthora pathogens onapple crops. The objectives of this research were to identifify and to study the potency of antagonist mold for inhibite the growth of Phytophthora spp. pathogens on apple crops. The Phytophthora spp. were isolated using baiting method with apple fruitÂ while antagonist molds were isolated by dilution method from soil sample of apple plantation. The inhibition assay of antagonist molds against Phytophthora was carried out by dual culture method. The isolated both of Phytophthora spp. and antagonist mold isolates were identified based on phenotype characters. The research result showed six molds namely Trichoderma sp.1, Trichoderma sp.4, Trichoderma sp.6, Aspergillus sp.2, Aspergillus sp.3 and Penicillium sp.1 which were antagonist on the Phytophthora pathogens onapple crops. Among the antagonist mold species, Trichoderma sp.6 had the highest inhibitory potency (62.95 %) while Penicillium sp.1 had the lowest inhibitory potency (28.82 %) against the growth of Phytophthora.Â

Ethnobotany of Medicinal Plants in Bromo Tengger Tribe Area, Bromo Tengger Semeru National Park Serafinah Indriyani Indriyani; Jati Batoro; Gustini Ekowati
Natural B, Journal of Health and Environmental Sciences Vol 1, No 3 (2012)
Publisher : Natural B, Journal of Health and Environmental Sciences

The research on use of various plants for medical purposes was undertaken in Tengger tribe area, Bromo Tengger Semeru National Park (BTSNP). The objectives of the research were to give information about species and potency of medicinal plants. Ethno-direct sampling was done through directly or semi-structural interview to the ordinary peoples, traditional leader and traditional therapist. The result of study showed that there were 98 species of medicinal plants that used by Tengger purwoceng (Pimpinella pruatjan), krangean (Abrus laevigatus), adas (Foeniculum vulgare), kayu ampet (Astronia macrophylla), pulosari (Alyxia reinwardtii), pronojiwo (Euchresta horsfieldii), sempretan, dringu (Acorus calamus) dan jamur impes were endemic grow in BTSNP. Tengger tribe did not cultivate the medicinal plants by themselves but seeking it in BTSNP forest.

Antimalarial Activity (Plasmodium falciparum 3D7) from Sea Sponge Acanthella sp. faidzil Ikram; Asep Awaludin Prihanto; Choiriyatun Hanifah; Muhammad Iqbal
Natural B, Journal of Health and Environmental Sciences Vol 1, No 3 (2012)
Publisher : Natural B, Journal of Health and Environmental Sciences

The objective of the research was to get the bioactive candidates from marine sponge Acanthella sp. for anti multi resistant malaria. The first Research was conducted with isolating the active compounds of Acanthella sp. In vitro antimalarial test against Plasmodium falciparum 3D7 was done with several concentrations with positive control using chloroquinon the quantification of the Scizon inhibition was performed with blood staining. Afterward, the infected erythrocytes per 1000 erythrocytes was calculated. IC50 was assayed with probit analysis. The result indicated that all four active fractions showed inhibition on growth of P. Falciparum with different value on IC50. The best fraction is confirmed by fraction B with IC50 of 0.013 ppm.

Avalanche Potential Analysis using Resistivity Method and USLE Method on Ranu Pani Area, Senduro Sub-district of Lumajang Regency Siswo Purnomo; Sunaryo Sunaryo; Luchman Hakim
Natural B, Journal of Health and Environmental Sciences Vol 1, No 3 (2012)
Publisher : Natural B, Journal of Health and Environmental Sciences

Has done research in the Ranu Pani area is one of the area where landslides have its potentiality, due to its location on the edge of the mountains where the soil is clay, slightly, sandy and has a very high fertility rates. One of the methods used to analyze landslide potential is using USLE (Universal Soil Loss Equation). In addition, the resistivity method with dipole-dipole configuration is also used to determine the structure of the soil and slip areas in the hills in the area Ranu Pani. Debris flow obtained from calculations using the USLE method in the second trajectory is equal to 796.5 tons/year, and the third trajectory is equal to 291.506 tons/year. According to the classification level of erosion, Ranu Pani area is an area with very dangerous level of erosion hazard, because the value of debris flow is more than 180 tons/year. With the high value of debris flow erosion, causing higher sedimentation of the lake, because the Ranu Pani lake accommodate most of the debris flow erosion of the hills around it.

Earthquake Characteristics of Mount Semeru Eruption, East Java Year 2009 Cholisina Anik Perwita; Sukir Maryanto; Agus Budianto
Natural B, Journal of Health and Environmental Sciences Vol 1, No 3 (2012)
Publisher : Natural B, Journal of Health and Environmental Sciences

Type of eruption during the year 2009 can be classified into three types. Type I is a visual explosion that manifested by the eruption of contain volcanic material. Type II is the eruption of the manifestated by fog. Type III is the eruption that occurred during lava dome growth. Spectral and hypocentre analysis has been done used four seismic stations Kepolo (KPL), Leker (LEK), Tretes (TRS) and Besuk Bang (BES). From the analysis found that the correlation between visual eruption and frequency indicates that the seismic eruptions have type I has a frequency 0.87 to 3.8 Hz frequency, seismic eruption of type II has a frequency 0.87 to 2.64 Hz frequency, while the seismic eruptions type III has a frequency 1.06 to 2.73 Hz. Result of study shows hypocenter is located at a depth of 0.90-1.47 km below the crater, interpreted as shallow magma pockets. Described based on the seismicity of 2009. Activities eruption caused by changes of pressure in deep and shallow magma pockets. The eruption process is more controlled by increase of pressure in the shallow magma pockets

Cell T Regulator CD4 + CD25 + Increasing Tolerance of Donors and Recipients on Allogenic Transplantation Muhaimin Rifa’i
Natural B, Journal of Health and Environmental Sciences Vol 1, No 3 (2012)
Publisher : Natural B, Journal of Health and Environmental Sciences

To gain transplantation tolerance between donor and recipients is the ultimate goal of all transplantations, either solid or bone marrow transplantation. Activation and induction of regulatory T cells are shown to increase tolerance between donor and recipient. This study describes the importance of regulatory T cells, CD4+CD25+ in allogeneic bone marrow transplantation. Injection of bone marrow cells as much as (3-5) x 105 shown to increase donor T cell engraftments in recipient, both CD4 and CD8 populations. Naive T cells, CD4+CD62L+ cells increased in recipients receiving bone marrow co-transfer with regulatory T cells. In this study also demonstrated that granulocytes and B cells can better develop on a recipient that receives co-transfer of regulatory T cells. Interestingly, the cells that are resistant to radiation remain on the peripheral and does not interfere with the bone marrow. The results of this study could illustrate, at least partially, how naturally occurring Tregs exert their regulatory function in vivo as they constitute only 5 – 10% of peripheral CD4+ T cells.

Study of Ozone Synthesis With Methods of Plasma Discharge Rachmat Triandi Tjahjanto; Sri Wardhani; Ulfa Anggraini
Natural B, Journal of Health and Environmental Sciences Vol 1, No 3 (2012)
Publisher : Natural B, Journal of Health and Environmental Sciences

In this research an ozonator prototype has been built to produce ozone, consists of air pump, gas flowmeter, an ozone reactor, and a glass pipe of nozzle. The most important part of the device is the reactor which consisting three main parts; 5 mm alloy electrode connected to a high voltage generator in the form of electrical gas stove lighter, 1 mm copper negative electrodes connected to the ground and a glass plasma discarge. The ozone gas formed is flowed to a container consisting of methyl orange solution 20 ppm at wave lenght of 470 nm. The concentration of ozone is determined by spectrophotometry method. The ozonator is characterized by ozonation periode variation of 5, 10, 15, 20, 25, and 30 minutes with the voltage used 3,5 volt, voltage variation of 3, 4, and 5 volts and flow rate variation of 227, 363, 464, 662, 864, and 1060 mL/min at the voltage used 3.5 volt. The result showed that the longer period and the higher voltage used for ozonation, the higher the ozone gas produced. The concentration of ozone obtained in this research is 0.76 ppm reached at 363 mL/min with 20 minutes ozonation duration.

Effects of Visible Spectrum on Potential Differential Response to Soybean Sprouts (Glycine max (L.) Merill) during photosynthesis Farida Wahyuni; Unggul P. Juswono; Kusharto Kusharto
Natural B, Journal of Health and Environmental Sciences Vol 1, No 3 (2012)
Publisher : Natural B, Journal of Health and Environmental Sciences

The absorption visible light spectrum by chlorophyll cause excited electron. The transition of electron in photosynthesis process result electrical potential difference response which can be utilized to spur metabolism process in the plants itself. This research used the seedling of Glycine max (L.) and the varietas is sindoro. The measurement of the potential difference response is done by adding of visible light (red, yellow, green, blue and violet) alternately to the bean sprouts of Glycine max (L.) Merill. The light distribution is being done for 1 second, 5 second and constantly. The result of the research showed the effect of the visible light distribution constantly in the photosynthesis process. In the red light occurs maximum potential difference response. In peak I resulted -147,3 mV and peak II resulted -137 mV. The minimum potential difference response is resulted by green light. in peak I resulted -68,8 mV and peak II resulted -56,75 mV. The effect of the visible light distribution during any time in the photosynthesis process. In the red light occurs maximum potential difference response. In peak I resulted -119,2 mV for 1 second and -146 Mv for 5 second. In peak II resulted - 112,3 mV for 1 second and -125,5 mV for 5 second. The minimum potential difference response is resulted by green light. In peak I resulted -64,75 mV for 1 second, -69,5 mV for 5 second, and in peak II resulted -55,9 mV for 1 second and -56 mV for 5 second.

Utilization of Liquid Emulsion Membranes To Set Aside Nickel Ions In Liquid Waste Bambang Ismuyanto
Natural B, Journal of Health and Environmental Sciences Vol 1, No 3 (2012)
Publisher : Natural B, Journal of Health and Environmental Sciences

Emulsion liquid membrane containing Di-(2ethylhexyl) phosphoric acid (D2EHPA) have been applied to remove nickel ion from wastewater of electroplating industry. Membrane was made from 0.1 mL of Span – 80; 0.6 mL of D2EHPA and 9.3 mL kerosene. Internal phase was made from 5 mL HCl 0,1 N, while the external phase that was nickel solution varied on concentration for 100-300 ppm. The separation process was done by stirring the solution in various rate of 100-250 rpm at 4 minutes. The last concentration of nickel was measured by using visible spectrophotometry at wavelength 470 nm by dimethylglyoxime method. From the experimental results, it has been established that increasing in stirring rate could increasing permeated nickel ion value. While increased on concentration of external phase also promoted permeated nickel ion value, with highest value 75%.

General Spatial Models (GSM) Approach on Baby Infant Mortality Data Henny Pramoedyo; Meilinda Trisilia
Natural B, Journal of Health and Environmental Sciences Vol 1, No 3 (2012)
Publisher : Natural B, Journal of Health and Environmental Sciences

Proximity and linkages between sites led to the emergence of the phenomenon of spatial linkages. Weighting matrix can be used to determine the proximity and linkages between spatial data or spatial relationships and can be used to calculate the coefficient of spatial dependencies. This study uses spatial panel data, namely the infant mortality rate (IMR) regional data taken from a unit area of development (SWP) Gerbangkertasusila and the Malang-Pasuruan SWP in the period 2005-2009. Those data use rook contiguity to make spatial weighting matrix. The aim of this study is to determine the model of what can be formed from the general spatial model (GSM) using panel data. Estimation of panel models with common effects approaches, fixed effects and random effects, will be followed by estimating the coefficient parameters of the general spatial model on panel data using maximum likelihood estimation method. From the prediction model by using software EViews7 note that all spatial panel data in this study followed the random effect model. To estimate the coefficient parameters of the general spatial model with Matlab-R2010 software is used to obtain a spatial lag / autoregressive models (SAR) of random effects and spatial error models (SEM) random effect. Model selection using the criteria as well as the largest R2 and corr2, and the smallest AIC values, the MSE values and the SC values. The best model for regional infant mortality data is the spatial error models (SEM) random effect.

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