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Natural B
Published by Universitas Brawijaya
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Articles 14 Documents
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Search results for , issue "Vol 2, No 1 (2013)" : 14 Documents clear
Effect of Adding Ions - Metal Ions to Pectinase Activity Isolation from Bacillus Subtilis on Bleaching Paper Mardiana Prasetyani Putri; Anna Roosdiana; Sasangka Prasetyawan; Diah Mardiana
Natural B, Journal of Health and Environmental Sciences Vol 2, No 1 (2013)
Publisher : Natural B, Journal of Health and Environmental Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (155.888 KB) | DOI: 10.21776/ub.natural-b.2013.002.01.11

Abstract

Pectinase enzyme used as biocatalyst to overhaul pectat compounds or pectin. Pectinase avtivity was affected by metal ions. The purpose of this study is to determine the effect of adding metal ions on pectinase activity and its inhibition type.. Determination of enzyme activity was based on galacturonic acid produced, which was analyzed by spectrophotometric using DNS reagent. Concentration of metal ions used i.e. Zn2+, Cu2+, Fe3+ were 2-10 mM. The result showed that Vm and KM number without ions were 161.29 µmol/mL minutes and 0.55%(w/v), respectively. While the presence of ions Zn2+, Cu2+, Fe3+ were producing Vmapp 147.06; 156.25 and 153.85µg.ml-1min-1, KMapp of 0.56%; 0.63% and 0.65%, and also KI of 333,33; 41,38; 32,9. The inhibition to metal ions are non-competitif inhibition.
Growth of Probiotic Bacteria (Bifidobacterium bifidum and Lactobacillus plantarum) in Medium Mixed Taro Rope (Colocasia esculenta L. Schott var Boring) and Soybean Powder Joni Kusnadi; Kartika Cahyania Pratiwi
Natural B, Journal of Health and Environmental Sciences Vol 2, No 1 (2013)
Publisher : Natural B, Journal of Health and Environmental Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (155.791 KB) | DOI: 10.21776/ub.natural-b.2013.002.01.12

Abstract

This study was aimed to determine the effect of mix proportions of soy powder and taro flour on the growth of probiotic bacteria Bifidobacterium bifidum and Lactobacillus plantarum. The experiments were performed using a randomized block design with two factors: the proportion of taro flour: soybean powder on the fermentation medium (6%: 9%, 7.5%: 7.5%, and 8%: 7%) and type of probiotic bacteria (B. bifidum and L. plantarum) with 3 replications. The results showed that treatment of taro flour and soybean powder on fermentation medium significantly (α = 0.05) decrease the degree of acidity (pH). While the treatment of probiotic (B. bifidum and L. plantarum) isolates significantly (α = 0.05) increase the total BAL, reduced the degree of acidity (pH), decreased total sugar and starch content. There is no interaction between the two treatments. The best treatment was obtained from the proportion of taro flour fermentation medium: soybean powder of 8%: 7% for L. plantarum isolates with the total L. plantarum of 4,24x1010CFU/ml, the degree of acidity (pH) of 3.32, and total sugar and starch content of 19.29%, 6.53%, respectively.
Potential Soursop Leaf Extract, Soursop Seeds and Mahogany Seeds for Grayak Caterpillar Control (Spodoptera litura L.) Yanuwiadi, Bagyo; Leksono, Amin Setyo; H., Hiasinta Guruh; Fathoni, M.; Bedjo, Bedjo
Natural B Vol 2, No 1 (2013)
Publisher : Natural B

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (141.486 KB) | DOI: 10.21776/ub.natural-b.2013.002.01.13

Abstract

This research objective is to compare the effect of extracts of soursop leave, sousop seed and mahogany seed on stop feeding, mortality and surviving of pupae and imago of army worm (Spodoptera litura L.). Army worm larvae were collected from Kebun Percobaan Muneng Research Field, Probolinggo and transferred to laboratorium for rearing and treatment. Soursop leaves, soursop seeds and mahagony seeds were extracted by aquades extraction method. Bioassays were conducted by using five concentration variation applied on 10 larvae by dipping method with twice replication. Observation of stof feeding was conducted every 2 hours during 24 hours. Whereas, observation on mortality was done every 24 hours during 168 hours. Result showed that the effect of soursop leave extract on the stop feeding and mortality was better than that of soursop and mahogany seed. This effect was started from 33.3% at 16 HAA (hours after application) increased to 46% at 24 HSA. Interestingly, the effect on survivality of pupae and imago was highest on the application of mahagony extract. The failure of pupation was 70%, and that of imago was 76.6%. This indicates that the effect of soursop leave extract is better for sort time application but on the long-term, the effect of mahogany seed is better than the others. 
Synthesis of N, N-diethyl-2-Hydroxybenzamide Using Methyl Salicylate from Gandapura Oil Suratmo Suratmo; Rurini Retnowati; Devina Azzuhro
Natural B, Journal of Health and Environmental Sciences Vol 2, No 1 (2013)
Publisher : Natural B, Journal of Health and Environmental Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (412.583 KB) | DOI: 10.21776/ub.natural-b.2013.002.01.14

Abstract

The synthesis of N,N-diethyl-2-hydroxybenzamide used methyl salicylate from gandapura oil  was conducted. This study aims to find out the synthesis process of N,N-diethyl-2-hydroxybenzamide from methyl salicylate. Methyl salicylate from gandapura oil was mixed with 40% diethylamine solution in diethyl eter and 1% NaOH solution in methanol. The mixture was stirred at 0°C for 1 hour and continued for 24 hours at room temperature. Compound synthesized was separated and determined physical properties include color, shape, odor and melting point, and was identified by FT-IR spectrophotometer and Gas Chromatography-Mass Spectrometer (GC-MS). The results of identification by FT-IR spectrophotometer showed absorption at 1631.67 cm-1 which is the C = O stretching vibrations of amide. The identification results obtained by GC-MS 6 peak. Peak with a retention time of 26.948 minutes with the fragmentation pattern is thought to be the target compound (N, N-diethyl-2-hidroksibenzamida). Identification results from GC-MS obtained 6 peak, and peaks with the retention time of 14.363 minutes showed the presence of methylsalicylate compounds, whereas the peak with a retention time of 26.948 minutes is suspected to be the synthesized compounds (N,N-diethyl-2-hydroxybenzamide). Target compounds synthesized gained by 0.21% in the form of brown solid matter with a melting point of 106-112oC.

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