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Rachma Wikandari
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Faculty of Agricultural Technology, Universitas Gadjah Mada, Jl. Flora No. 1, Bulaksumur, Yogyakarta 55281, Indonesia
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INDONESIA
agriTECH
Published by Universitas Gadjah Mada
ISSN : 02160455     EISSN : 25273825     DOI : 10.22146/agritech
Core Subject : Agriculture,
Agriculture, Biological Sciences & Forestry
Agritech with registered number ISSN 0216-0455 (print) and ISSN 2527-3825 (online) is a scientific journal that publishes the results of research in the field of food and agricultural product technology, agricultural and bio-system engineering, and agroindustrial technology. This journal is published by Faculty of Agricultural Technology, Universitas Gadjah Mada, Yogyakarta in colaboration with Indonesian Association of Food Technologies (PATPI).
Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ilmu Pertanian dan Biologi (Lain-Lain)
Articles 6 Documents
 1 
Search results for , issue "Vol 23, No 2 (2003)" : 6 Documents clear
Characterization of Extra-and Intracelullar from Proliverating Yeast during Tempeh Production Sutardi Sutardi
agriTECH Vol 23, No 2 (2003)
Publisher : Faculty of Agricultural Technology, Universitas Gadjah Mada, Yogyakarta, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1753.085 KB) | DOI: 10.22146/agritech.13513

Abstract

Proliferating yeast species such as Endomycopsis burtonii, Candida diddensiae and Candida iropicalis, produced both extra- and intracellular phytases during tempeh (soaking of soybean) production. The enzymes were isolated from growth media and the cultured yeast. and partially purified by acetone fractionation. Extracellular phytase activities were higher than that of the intracellular enzymes. The extra- and intracellular phytases of E. burtonii., C. diddensiae and C. iropicalis acted over a narrow pH ranging at 3.2 - 4.8 and the maximum activity at temperatures between 45° - 70°C. Estimated K„, values for extra- and intracellular phytases from yeast were within the range 0.5 - 3.1.x 10' M. The V„,„„ values were within the range 0.004 - 0.19 }mole P, liberated/min/m1 enzyme for the extra- and intracellular phytases of E. burtonii, C. diddensiae and C. iropicalis. The activation energy for hydrolysis of phytic acid by extra- and intracellular phytases of E. burtonii, C. diddensiae and C. iropicalis were calculated by,the Arrhenius equation being 7,100; 9,100; 13,00, 5,600, 7,100 and 21,700 cal/mole, respectively.
Isolasi dan Seleksi Lactobacillus yang Berpotensi sebagai Agensia Probiotik Siti Nur Purwandhani; Endang Sri Rahayu
agriTECH Vol 23, No 2 (2003)
Publisher : Faculty of Agricultural Technology, Universitas Gadjah Mada, Yogyakarta, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (2094.799 KB) | DOI: 10.22146/agritech.13514

Abstract

Lactic acid bacteria are important in food fermentation, in producing antimicrobial substances responsible for food preservation, and in balancing the microflora composition in gastrointestinal tract which contributing many healthful benefits (as probiotic agent). Disturbance due to pathogenic bacteria colonization in intestine as well as sterilisation of intestine due to antibiotic ingestion can be overcome by consumption of probiotic lactic acid bacteria. The objective of this study was to isolate Lactobacillus which potential for probiotic agent from intestinal material of healthy infant baby. Isolation was conducted using peptone glucose yeast extract media added with 0,2 % oxgall at pH 5, followed by incubation at 37 0C for 48 hr. Identifications were carried out based on Grain, morphological, biochemical and physiological characters, peptidoglycan types and protein profile on SDS-PAGE. Selection of probiotic agents were on based their antagonisms toward pathogenic bacteria, their resistance to antibiotics, and their survival at different oxygen availabilities. Based on morphological, biochemical and physiological characters among 12 lactobacilli strains obtained during isolation, 8 of them were identified as Lactobacillus acidophilus and the rest as Lactobacillus reuteri. However based on protein profile, L. acidophilus group has two different profiles, the first, consist of 7 strains and the second, consists of one strain. All isolates inhibited the growth of pathogenic bacteria including Shigella sp., Escherichia coli sp., Escherichia coli FNCC 009 I, Proleus sp., Salmonella choleraesius JCM 3919, Staphylococcus aureus FNCC 0047, Vibrio parahaemoliticus JCM 2147, Bacillus cereus ATCC 0057 and Listeria monocytogenes ATCC 7644, as shown by the inhibition zone ranging from 0,5-8 mm. Eight isolates of L. acidophilus were not resistant to antibiotics tested, while 3 isolates of L. reuteri were not resistant to chloramphenicol, rifampin and ampicillin, however they were resistant to tetracycline and elkosin. At reduced oxygen and anaerobic conditions all the isolates grew well, but at aerobic condition the growth was relatively slow. Strains of Lactobacillus acidophilus could be used for probiotic agents.
Lactic Acid Bacteria in Fermented Foods of Indonesian Origin Endang Sri Rahayu
agriTECH Vol 23, No 2 (2003)
Publisher : Faculty of Agricultural Technology, Universitas Gadjah Mada, Yogyakarta, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1480.25 KB) | DOI: 10.22146/agritech.13515

Abstract

In this study, about 194 lactic acid bacteria strains have been isolated from 21 kinds of fermented foods (plant materials and fish origin). These fermented foods were salted-fermented fruits, vegetables & fish; fermented raw cassava (gatot & growol); tape (cassava & glutinous rice); microbial starter cultures (ragi); and fermented soybean (tempe & moromi). Among these strains, 109 belong to genus Lactobacillus, which dominated by homofermentative Lactobacillus plantarum pentosus, 25 strains belong to Pediococcus (mostly P. pentosaceus acidilactici), 45 strains Streptococcus which mostly identified as Streptococcus thermophilus, 7 strains belong to Enterococcus, which further identified as E. faecium and 8 strains Leuconostos (Weisella) as Weisella paramesenteroides. Lactobacilli have been found in all fermented foods, pediococci in 11 kind of fermented foods both plant material and fish origin, while streptococci mostly found in fermented fish, as well as enterococci and Leuconostoc. Nine strains belong to Lactobacillus plantarum - pentosus complex from different fermented food samples have been determined their DNA-DNA homology to L. plantarum NRIC 1067 and L. pentosus NRIC 1069. Result of their homology to these strains shown that all these nine strains are identified as L. plantarum. Lactic acid bacteria from Indonesian fermented foods are dominated by Lactobacillus plantarum, followed by Pediococcus pentosaceus, and Streptococcus thermophilus.
Sistim Informasi Operasi Jaringan Irigasi untuk Modernisasi Pengelolaan Irigasi serta Peningkatan Proses Dialogis Petani dengan Pemerintah Wisnu Wardana; Sigit Supadmo Arief; Darmadi Darmadi; Anjar Suprapto
agriTECH Vol 23, No 2 (2003)
Publisher : Faculty of Agricultural Technology, Universitas Gadjah Mada, Yogyakarta, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (2893.908 KB) | DOI: 10.22146/agritech.13516

Abstract

Managing irrigation water is not only distribute irrigation water .to the assigned field, but it is also include arranging the cropping pattern and scheduling in order to fit it to the availability of irrigation water. Cropping pattern and scheduling plan is the important things because each crop has its characteristics in water consumptions and on the other hand there is a limited water resources. There are three important factors in determining of cropping pattern and scheduling: (i) farmers need, (ii) service target and (iii) the ability of irrigation scheme. in the era of Government Regulation No 77/01, farmers participation in operation and maintenance (O&M) of irrigation scheme is an important things. The consequences is the managers of irrigation scheme have to negotiate these cropping pattern and scheduling plan as well as its water allocation to the farmers. The aim of this study is to prepare the tool to make easier during negotiation for determining the cropping pattern and scheduling. This tool is a computer program of information systems used for O&M of an irrigation scheme, and it is based on the availability of irrigation water, irrigation scheme performance, crop characteristics as well as its water consumption, market driven as well as the income offarmers.
Occurrence and Detoxification of Mycotoxinsin Food Sardjono Sardjono
agriTECH Vol 23, No 2 (2003)
Publisher : Faculty of Agricultural Technology, Universitas Gadjah Mada, Yogyakarta, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1037.814 KB) | DOI: 10.22146/agritech.13517

Abstract

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Kajian Kehandalan Teknologi Irigasi Air Tanah untuk Usaha Tani Sistem Sorjan di Kabupaten Kulonprogo Muhjidin Mawardi
agriTECH Vol 23, No 2 (2003)
Publisher : Faculty of Agricultural Technology, Universitas Gadjah Mada, Yogyakarta, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1360.139 KB) | DOI: 10.22146/agritech.13518

Abstract

A technology of ground water irrigation for sorjan farming system has been applied by farmers in Kecamatan Panjatan, Kabupaten Kulonprogo for years. The technology is simple and indigenous but there is no such infOrmation concerning it reliability and appropriateness. The aims of the study are to examine the reliability of the ground water irrigation for soda', farming Spiem and factors influence performance of the technology. The reliability is examined by the productivity of land and waler use, irrigation effectiveness and farmers income from the sorjan farming system. The study is conducted by survey and field observation method, includes physical characteristics of the sorjan land, ground water level, interval of wells ernd cropping pattern. Labour used for irrigation perday, time consumed for one irrigation, irrigation capacity perday and irrigation effectiveness are observed in the field during the working days. Crops production and irrigation cost, crops yield and famers income from sorjan farming are investigated through field survey. Two locations of sorjan farming are selected as the the sample locations for the study. The study concludes that the sorjan farming under crops rotation of rice and vegetable in the furrows and vegetable crops for all season on the ridges performs as a productive and adaptive land and water use for agriculture. The technology of ground water irrigation for the sorjan farming is considered as a reliable technology from the point view of adaptability to the local environment, water availability, irrigation effectiveness and farmers income per year from sorjan farming . Besides that. the technology is developed by the local farmers based on their wise and experiences to local circumstances and therefore, more appropriate and sustainable.

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