cover
Article Per Year (5 Year)
All
Home Page
OAI Link
Editorial Team
Contact
Reviewer
Google Scholar
Contact Name
-
Contact Email
-
Phone
-
Journal Mail Official
-
Editorial Address
-
Location
Kab. jember,
Jawa timur
INDONESIA
Journal of Agromedicine and Medical Sciences
Published by Universitas Jember
ISSN : 24609048     EISSN : 27145654     DOI : -
Core Subject : Health,
Health Professions
Jurnal Agromedicine and Medical Sciences (AMS) adalah jurnal berkala empat bulan (Februari, Juni dan Oktober) yang berisi berbagai artikel dalam bentuk penelitian, tinjauan sistematis dan laporan kasus dalam bidang kedokteran dengan fokus pada ilmu-ilmu kedokteran dasar, kedokteran klinis dan agromedis.
Arjuna Subject : -
Articles 11 Documents
 1   2 
Search results for , issue "Vol 8 No 3 (2022)" : 11 Documents clear
Low Oxygen Tension Inhibits Senescence in Bone Marrow Mesenchymal Stem Cells (BMSCs) Candra Bumi
Journal of Agromedicine and Medical Sciences Vol 8 No 3 (2022)
Publisher : Faculty of Medicine, University of Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/ams.v8i3.33843

Abstract

Background: Stem cells have been used in regenerative medicine but are so few in the body that they require cell culture. Stem cell culture was performed under normal oxygen tension and passage was carried out until the number of cells was sufficient for therapy. Stem cell cultures under normal oxygen tension do not match the stem cell microenvironment, which can lead to premature senescence. This study aims to determine the association of low oxygen tension with premature senescence of mesenchymal stem cells (MSCs) through inhibition of p21 expression by HIF-1a. Methode: The research method used rabbit bone marrow in New Zealand as a source of MSCs. The results of isolation of MSCs were divided into two groups for cultured on normal and low oxygen tension until 10 passages. Cells were identified using flowcytometry for cd105 and cd34. At early and late passage, the expression of p21 and HIF-1a was examined using immunofluorescence while senescence was examined using β-galactosidase assay. Results: The results showed that in low oxygen cultures HIF-1a expression increased significantly (p <0.05) while p21 expression decreased significantly (p <0.05) as did the β-galactosidase assay. Conclusions: The conclusion of this research is low oxygen tension culture able to decrease premature senescence culture of invitro stem cells mesenchymal through obstacles p21 by HIF-1a. Keywords: p21 expression, HIF-1a expression, late passage, premature senescence.

Page 2 of 2 | Total Record : 11

 1   2 

Filter by Year

2022 2022


Reset
Filter By Issues
All Issue Vol. 11 No. 3 (2025) Vol. 11 No. 2 (2025) Vol. 11 No. 1 (2025) Vol 10 No 3 (2024) Vol. 10 No. 3 (2024) Vol 10 No 2 (2024) Vol 10 No 1 (2024) Vol 9 No 3 (2023) Vol 9 No 2 (2023) Vol 9 No 1 (2023) Vol 8 No 3 (2022) Vol 8 No 2 (2022) Vol 8 No 1 (2022) Vol 7 No 3 (2021) Vol 7 No 2 (2021) Vol 7 No 1 (2021) Vol 6 No 3 (2020) Vol 6 No 2 (2020) Vol 6 No 1 (2020) Vol 5 No 3 (2019) Vol 5 No 2 (2019) Vol 5 No 1 (2019) Vol 4 No 3 (2018) Vol 4 No 2 (2018) Vol 4 No 1 (2018) Vol 3 No 3 (2017) Vol 3 No 2 (2017) Vol 3 No 1 (2017) Vol 2 No 3 (2016) Vol 2 No 2 (2016) Vol 2 No 1 (2016) Vol 2, No 1 (2016) Vol 1, No 2 (2015) Vol 1 No 2 (2015) Vol 1 No 1 (2015) Vol 1, No 1 (2015) More Issue