cover
Article Per Year (5 Year)
All
Home Page
OAI Link
Editorial Team
Contact
Reviewer
Google Scholar
Contact Name
Y. Andi Trisyono
Contact Email
anditrisyono@ugm.ac.id
Phone
+62274-523926
Journal Mail Official
jpti.faperta@ugm.ac.id
Editorial Address
Jalan Flora No. 1, Bulaksumur, Sleman, Yogyakarta, 55281
Location
Kab. sleman,
Daerah istimewa yogyakarta
INDONESIA
Jurnal Perlindungan Tanaman Indonesia
Published by Universitas Gadjah Mada
ISSN : 14101637     EISSN : 25484788     DOI : -
Core Subject : Agriculture,
Agriculture, Biological Sciences & Forestry
Arjuna Subject : -
Articles 20 Documents
 1   2 
Search results for , issue "Vol 23, No 2 (2019)" : 20 Documents clear
Bacillus subtilis from Potato Rhizosphere as Biological Control Agent and Chili Growth Promoter Nur Prihatiningsih; Heru Adi Djatmiko; Erminawati Erminawati; Puji Lestari
Jurnal Perlindungan Tanaman Indonesia Vol 23, No 2 (2019)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.40606

Abstract

Bacillus subtilis is an antagonist bacteria  that inhibits the growth of fungal and bacterial plant pathogens. The B. subtilis has roles as biocontrol agents and plant growth promoting rhizobacteria (PGPR). This research aimed to evaluate the potency of B. subtilis isolates (B209, B211, and B298) as a biocontrol agent to anthracnose (caused by Colletotrichum spp.) and as PGPR to increase the growth of chili plants. The experiments were divided into two batches. The first batch was conducted in the laboratory to evaluate the characteristics of B. subtilis (as biocontrol) and PGPR (phosphate solubility, producing IAA and nitrogen). The second batch was conducted in the field in Rempoah Village, Baturraden Regency, Banyumas District, with 5 treatments and 6 replications. Parameters observed were inhibition percentage to Colletotrichum spp., disease intensity, the component of PGPR, plant growth of chili, and phatosystem. The results showed that B209, B211, and B298 isolates inhibited the growth of Colletotrichum spp, with the highest inhibition percentage on B298. B209, B211, and B298 have characteristics as PGPR, i.e. the ability to soluble phosphate, to produce IAA and nitrogen. The ability of B298 to promote plant growth was shown by the increase of plant height, leaf number, plant dry mass, and dry root mass (38.0%, 54.7%, 61.7%, 61.8%, respectively). B298 and B211 could increase the fresh crop mass (41.2% and 37.1%) and fresh root mass (36.4% and 34.4%). B298 and B209 were similar in increasing the root length (25.2%). Root volume could be increased by 33.3% by applying B211 isolate. B209 was the best isolate to reduce anthracnose up to 80.36%.
Molecular Characterization of Begomovirus on Cucumber in Java Listihani Listihani; Tri Asmira Damayanti; Sri Hendrastuti Hidayat; Suryo Wiyono
Jurnal Perlindungan Tanaman Indonesia Vol 23, No 2 (2019)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.41402

Abstract

A survey on several cucumber cultivation areas in West Java, Central Java, Yogyakarta, and East Java found many plants showing typical Begomovirus symptoms such as yellow mosaic, cupping, and vein banding. This study was aimed to determine disease frequency, detection and molecular characterization of the causal virus of those symptoms on cucumber in Java. Sampling was conducted by purposive sampling by collecting 50 symptomatic plants from each location in West Java (Indramayu, Subang, and Bogor), Central Java (Brebes and Klaten), Yogyakarta (Kulon Progo), and East Java (Nganjuk, Kediri, and Tulungagung). The detection and disease frequency was determined based on DIBA test using a specific antiserum of Tomato leaf curl New Delhi virus (ToLCNDV) and Squash leaf curl virus (SLCV). The identification of nucleic acid was conducted by PCR using specific primer of ToLCNDV and SLCV, DNA cloning, and sequencing. The results of serological detection showed the disease frequency of ToLCNDV and SLCV ranged from 92.77-100% and 78.33-93.3%, respectively. PCR using specific primer of ToLCNDV successfully amplified the coat protein gene at a size of 600 bp from all samples. Homology nucleotide and amino acid sequences among ToLCNDV Java isolate ranging from 95.6-99.2% and 99.7-100%. ToLCNDV isolates Java had highest nucleotide and amino acid sequences similarity with cucumber isolate from Klaten, Indonesia (AB613825) ranging from 96.1-98.1% and 99.7-100%, and was considered as “Indonesia” strain. SLCV not amplified on all samples by PCR using specific primer, indicating it might not present yet on cucumber in Java.
Molecular Characterization of Betasatellite Associated with Begomovirus on Ageratum conyzoides in Magelang, Central Java Argawi Kandito; Sedyo Hartono; Sri Sulandari; Susamto Somowiyarjo
Jurnal Perlindungan Tanaman Indonesia Vol 23, No 2 (2019)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.46579

Abstract

Ageratum conyzoides is one of the common weeds in Indonesia. The presence of weed can decrease plant yield by resource competition and its role as alternative hosts for pests and diseases, especially begomoviruses transmitted by whitefly. The aim of this research is to detect and characterize the begomovirus-beta satellite in A. conyzoides. A. conyzoides showed severe yellowing symptoms were collected from Magelang, Central Java, Indonesia. Total DNA was extracted and analyzed using PCR method for begomovirus and beta satellite. Begomovirus detection was performed with universal primer Krusty-Homer, resulted a 500 bp DNA fragment. Betasatelit detection performed with specific primer β01/β02, resulted a 1300 bp DNA fragment, indicated presence of a beta satellite associated with the begomovirus. The sequence of begomovirus showed 95% similarity with Tomato Leaf Curl Java Virus (ToLCJaV). The sequence of beta satellites showed 85% similarity with Tomato Leaf Curl Betasatellite (ToLCB). Characterization of beta satellite showed three main characters of beta satellite, TAATATTAC stem-loop structure, Adenine-rich region, and 118 amino acids of putative ORF BC1. 
Antifungal Potency of Secondary Metabolites Produced by Endophytic Bacteria against Pathogenic Fungi Pyricularia oryzae Cav. Fitri Widiantini; Mia Rahmah Qadryani; Fuji Hartati; Endah Yulia
Jurnal Perlindungan Tanaman Indonesia Vol 23, No 2 (2019)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.48392

Abstract

Blast disease caused by Pyricularia oryzae Cav. is one of the most important diseases on rice. One of the alternative controlling methods in P. oryzae is biological control through the utilization of secondary metabolites produced by endophytic bacteria. The study aimed to determine the antifungal potency of secondary metabolites produced by rice endophytic bacteria against P. oryzae. The experiment was conducted using 9 endophytic bacteria isolated rice (Os1, Os2, Os3, Os4, Os5, Os6, Os7, Os8, and Os10). Each isolates were grown in ISP2 liquid media and the secondary metabolites compounds were extracted using two different solvents; methanol and ethyl acetate : methanol (4:1) (v/v). The effect of secondary metabolites was tested using agar well diffusion method. The results demonstrated that the secondary metabolites extracted by both solvents have antifungal effect on the growth of P. oryzae. The highest growth inhibition was shown by secondary metabolites extracted by ethyl acetate : methanol (4:1) from Os1 (42%) and Os3 (39%). Antifungal activity of the secondary metabolites was indicated by the formation of clear zone. HPLC (High Performance Liquid Chromatography) analysis showed the differences of peaks and retention time between secondary metabolites produced by Os1 and Os3 which has antifungal activity and secondary metabolites produced by Os10 that did not show the antifungal activity.
The Effect of Planting Distance and Depth of Water Puddle to Damage Intensity by Rice Yellow Stem Borer (Scirpophaga incertulas Walker) (Lepidoptera: Crambidae) in Relation to Microclimate Change Ulfah Nuzulullia; Edhi Martono; Y. Andi Trisyono
Jurnal Perlindungan Tanaman Indonesia Vol 23, No 2 (2019)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.28612

Abstract

The rice yellow stemborer (Scirpophaga incertulas) is one of main pests on rice which may attack at all growth stages. The weather was considered one of influencing factors against its infestation. Therefore, this research was conducted to recognize microclimate factors affecting the infestation of S. incertulas. The experiment was carried out in Institute for Pest Forecasting, Jatisari Karawang from January-April 2017. The investigation was arranged in Factorial of Blocked Randomized Design with four repetitions and  combinations of treatment factors, i.e. planting distance (conventional and jajar legowo 2 : 1) and depth of puddle (5 cm, 0 cm, and intermittent). The observation was performed from one to 12 weeks after planting. At the same time, three weather factors (temperature, relative humidity and rainfall) were observed in four West Java regencies i.e. in Bekasi, Karawang, Subang and Indramayu. The results showed that the conventional and the jajar legowo schemes are influenced by the micro air temperature.  Both planting distance and depth of water puddle treatment were influenced by the micro air temperature. Microclimate factors which influenced the intensity of infestation were air temperature, humidity, water temperature, and light intensity. The weather factor showing effects  in Bekasi, Karawang, and Indramayu was rainfall. However, the three weather factors did not affect on the acreage of pest infestation in Subang.
Detection and Control Bacteria Cause Grain Rot Burkholderia glumae on Rice Syahri Syahri; Renny Utami Somantri; Priatna Sasmita
Jurnal Perlindungan Tanaman Indonesia Vol 23, No 2 (2019)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.37755

Abstract

Burkholderia glumae, before mid-2018, is categorized as plant quarantine pest A2 Group 1 that its existence has been detected in Indonesia. B. glumae  has been known to spread in the central production of rice in Java, Sumatra, Borneo dan Sulawesi. This review aimed to explain the strategies for B. glumae detection through its characteristics and to prevent the divergence  of this bacterium in Indonesia. The previous studies reported that the bacteria could reduce yield up to 75% and caused the decrease  of weight-grain or the increase  of empty grain. The disease intensity is affected by environmental and physiological factors such as warm temperature at nighttime and high rainfall intensity. The optimum temperature for the development of the disease is 30–35°C. Moreover, the pathogen could survive at a temperature of 41°C. The tropical area of 32-36°C are suitable for B. glumae. Recently, the effective control of the disease in the field has not been found yet. Meanwhile, early detection of the disease is not yet determined,  even though  it is necessary  to prevent its spread in rice cultivation in Indonesia. Detection of the disease by Agricultural Quarantine Agency as a frontline is needed to check the entry of the disease carried by the import activities of the seed. Detection in the suspected field by protection institutes through frequent surveillance in central production areas of rice should be considered  as an important task.. The effective techniques to prevent B. glumae are the use of resistant varieties, the practice of seed treatments (using antibacterial, bactericide, heat treatment or plant extract), and  the application of oxolinic acid to the crops.
Morphological and Molecular Characteristics of Pratylenchus coffeae from the Origin of Robusta Coffee Plantation in Malang, East Java Aris Budiman; Supramana Supramana; Giyanto Giyanto
Jurnal Perlindungan Tanaman Indonesia Vol 23, No 2 (2019)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.42481

Abstract

Pratylenchus coffeae is the most important plant-parasitic nematode in Robusta coffee plantations. Information regarding morphology, morphometric and molecular characters of P. coffeae has not been reported in Indonesia. This study is aimed to describe those characters of P. coffeae that attack Robusta coffee. Root samples were taken from Robusta coffee plantation in Malang, East Java. Nematode extractions was conducted using a mist chamber method. Morphology and morphometric characters were observed from the permanent nematode slides. Single nematode DNA extract was amplified at the D2D3 segment of 28S rRNA and ITS1-5.8S-ITS2 rRNA with universal primers. Amplicon was sequenced and analysed for phylogenetic tree relationships. Female morphological key character of P. coffeae observed are: lip with two annulations, four lateral lines, esophageal overlap with intestine ventrally, monodelphic, and truncated tail shape. Male spicules curved ventrally. Female morphometrics are: n=26, L = 556.4 μm, DGO = 2.4 μm, anterior gonad = 174.8 μm, a = 28.5, b = 6.1, b’ = 4.1, c = 20.1, c’ = 2.3, V = 81.7. A Male is smaller than a female with n=24, L = 505.9 μm,  a = 32.3, b = 5.5, b '= 3.9, c = 15.3, c' = 2.8 and T = 40.6. The molecular characters of P. coffeae were investigated for two isolates, namely SA1 and SA2.  Based on the D2D3 and ITS1-5.8S-ITS2 regions, isolate SA1 has similarity level of 99% and 97% to the P. coffeae  from NCBI. Similar result was shown by Isolate SA2 with similarity of 100% and 100% respectively. Phylogenetic tree analysis using Maximum Likelihood at the D2D3 segment of 28S rRNA and ITS1-5.8S-ITS2 regions showed that P. coffeae in this study was included in one clade with P. coffeae from several countries.
Detection and Development of Infestation Rate of Aphelenchoides besseyi on Various Rice Seed Varieties Nikmatul 'Azizah; Siwi Indarti; Ani Widiastuti; Y. Andi Trisyono
Jurnal Perlindungan Tanaman Indonesia Vol 23, No 2 (2019)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.45558

Abstract

Aphelenchoides besseyi is a seed borne nematode caused white tip disease. Infested rice seeds are beneficial inoculum source forA. besseyi widespread. The objective of this research was to determine the occurence and development of symptom level caused by A. besseyi in rice seed varieties. The research was done by detecting A. besseyi in rice seed using Hoshino and Togashi method and counting the nematode population in the seed; observing the incubation time and measuring disease incidence every week. The result revealed that A. besseyi was found in 16 of 17 varieties, i.e. R1 IR64, R2 IR64, R3 IR64, R1 Situbagendit, R2 Situbagendit, Inpari 33, R1 Memberamo, R2 Memberamo, R3 Memberamo, R1 Way Apo Buru, R2 Way Apo Buru, Mekongga, Pepe, Ketan, Sintanur, and Ciherang.A. besseyi infection increased every week in many varieties depend on quantitative (statistics) and qualitative (symptom) indicators. There were 4 varieties have to be investigate further because they were infested by A. besseyi>30 nematodes/100 seeds, i.e. R1 Way Apo Buru, R1 Situbagendit, Ketan and R3 IR 64. A practice that is also required is the elimination of A. besseyi inoculum in rice seed before cultivate.
The Resistance of Local Pigmented Rice Varieties against Bacterial Leaf Blight Tri Joko; Kristamtini Kristamtini; Sumarno Sumarno; Rofiq Andriyanto
Jurnal Perlindungan Tanaman Indonesia Vol 23, No 2 (2019)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.46902

Abstract

Bacterial leaf blight (BLB) caused by Xanthomonas oryzae pv. oryzae (Xoo) is an important disease in rice plants worldwide, including in Indonesia. The use of resistant variety against BLB is the best effort in disease control because it is considered as the most effective and environmentally friendly. This study aimed to evaluate the resistance of local varieties, red rice (cv. Sembada Merah) and black rice (cv. Sembada Hitam), against Xoo compared to white rice variety (Ciherang), commonly cultivated by the farmers. The study was conducted in a greenhouse and arranged in Factorial of Completely Randomized Design (CRD) with 5 replications and 2 treatment factors: variety (Sembada Hitam, Sembada Merah, and Ciherang) and inoculation (with and without inoculated by Xoo bacterial suspension). Xoo inoculation was performed by clipping the leaves that close to the reproductive phase when the heading begins to be produced. The disease intensity and Area Under Disease Progress Curve (AUDPC) over two weeks were used to determine the level of resistance of the tested plant varieties. The results showed that black rice and red rice had better levels of resistance against BLB compared to white rice. At two weeks after inoculation, Sembada Hitam had the lowest AUDPC value followed by Sembada Merah, while the Ciherang had the highest AUDPC value and was categorized as a susceptible variety to Xoo.
Arthropod Diversity in the Tea Plantation within Several Years after Pruning in Pagilaran, Central Java Muhammad Rizyan Anggra Hidayat; F. X. Wagiman; Arman Wijonarko
Jurnal Perlindungan Tanaman Indonesia Vol 23, No 2 (2019)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.47172

Abstract

The lush leaves of tea within several years after prune is expected to be followed by arthropod abundance and diversity. The study aimed to evaluate the arthropod abundance, diversity, evenness, and dominance, at the tea plantation within several years after the prune. The study was conducted at Pagilaran tea plantations, Central Java, from April to May 2018, at an altitude of 900 m asl. Arthropod samplings were done in four plots of tea plantation namely 1, 2, 3, and 4 years after pruning, hereafter they are called PY1, PY2, PY3, and PY4.  The plot area was approximately 1 ha. The arthropod specimens were collected using sweep net and pitfall trap every day for 6 consecutive days.  The arthropod identification and calculation were carried out in the Laboratory of Entomology, Faculty of Agriculture, Universitas Gadjah Mada. Analysis of variance was applied to determine the effect of PY on the Arthropod abundance. The Shannon Wienner index, the Evenness index, and the Simpson index, were used to evaluate the arthropod diversity, evenness, and dominance, respectively. The results showed that the PY significantly affected the arthropod abundance. The arthropod number collected from PY1 (27 individuals/50 swings) was significantly lower as compared to the other PYs, while arthropod numbers amongst PY2, PY3, and PY4 (53, 93, and 67 individuals/50 swings, respectively) were relatively similar. Out of the total number of 1.432 arthropod specimens, it comprised of 10 orders at which Hemiptera was the highest order (48.04%), and 69 families at which Cicadellidae was the highest family (32.12%). Amongst specimens of Cicadellidae, there was Empoasca sp. which is an important tea pest. The Shannon Wienner index (H) of orders and families ranged from 1.24 to 2.69 indicates moderate arthropod diversity. The Evenness index (e') ranged from 0.57 to 0.91) indicates the arthropod evenly distributed. Meanwhile, the Simpson index (D) ranged 0.10 - 0.39 indicates that there was no dominance of the order or family.

Page 2 of 2 | Total Record : 20

 1   2 

Filter by Year

2019 2019


Reset
Filter By Issues
All Issue Vol 29, No 2 (2025): In Progress Vol 29, No 1 (2025) Vol 28, No 2 (2024) Vol 28, No 1 (2024) Vol 27, No 2 (2023) Vol 27, No 1 (2023) Vol 26, No 2 (2022) Vol 26, No 1 (2022) Vol 25, No 2 (2021) Vol 25, No 1 (2021) Vol 24, No 2 (2020) Vol 24, No 1 (2020) Vol 23, No 2 (2019) Vol 23, No 1 (2019) Vol 22, No 2 (2018) Vol 22, No 1 (2018) Vol 21, No 2 (2017) Vol 21, No 1 (2017) Vol 20, No 2 (2016) Vol 20, No 1 (2016) Vol 19, No 2 (2015) Vol 19, No 1 (2015) Vol 18, No 2 (2014) Vol 18, No 1 (2014) Vol 17, No 2 (2011) Vol 17, No 1 (2011) Vol 16, No 2 (2010) Vol 16, No 1 (2010) Vol 15, No 2 (2009) Vol 15, No 1 (2009) Vol 14, No 2 (2008) Vol 14, No 1 (2008) Vol 13, No 2 (2007) Vol 13, No 1 (2007) Vol 12, No 2 (2006) Vol 12, No 1 (2006) Vol 11, No 2 (2005) Vol 11, No 1 (2005) Vol 10, No 2 (2004) Vol 10, No 1 (2004) Vol 9, No 2 (2003) Vol 9, No 1 (2003) Vol 8, No 2 (2002) Vol 8, No 1 (2002) Vol 7, No 2 (2001) Vol 7, No 1 (2001) Vol 6, No 2 (2000) Vol 6, No 1 (2000) Vol 5, No 2 (1999) Vol 5, No 1 (1999) Vol 4, No 2 (1998) Vol 4, No 1 (1998) Vol 3, No 2 (1997) Vol 3, No 1 (1997) Vol 2, No 2 (1996) Vol 2, No 1 (1996) Vol 1, No 1 (1995) More Issue