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INDONESIAN JOURNAL OF PHARMACY

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Published by Universitas Gadjah Mada

ISSN : 23389427 EISSN : 23389486 DOI : -

Core Subject : Health,

Medicine & Pharmacology

Indonesian Journal of Pharmacy (ISSN-e: 2338-9486, ISSN-p: 2338-9427), formerly Majalah Farmasi Indonesia (ISSN: 0126-1037). The journal had been established in 1972, and online publication was begun in 2008. Since 2012, the journal has been published in English by Faculty of Pharmacy Universitas Gadjah Mada (UGM) Yogyakarta Indonesia in collaboration with IAI (Ikatan Apoteker Indonesia or Indonesian Pharmacist Association) and only receives manuscripts in English. Indonesian Journal of Pharmacy is Accredited by Directorate General of Higher Education (DGHE) DIKTI No. 58/DIKTI/Kep/2013.

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Articles 8 Documents

Search results for , issue "Vol 14 No 2, 2003" : 8 Documents clear

ARTEMISININ PRODUCTION BY SHOOT CULTURE OF ARTEMISIA CINA BERG. EX POLJAKOV Maria Marina; Aziz Purwantoro; C. J. Soegihardjo
Indonesian Journal of Pharmacy Vol 14 No 2, 2003
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

Artemisinin is a secondary metabolite has a potential effect as an antimalaria. Research in artemisinin content in A. cina from shoot culture have not been reported. The aim of this research were:(i) to know the ability of A. cina shoot culture to produce artemisinin, (ii) to asses the effect of concentration of yeast extract, sucrose and their combination in promoting the production of artemisinin, (iii) to determine the highest artemisinin concentration produced by shoot culture in the medium containing different concentration of yeast extract, sucrose and their combination. The factorial of randomised complex block design (RCBD) was used in this experimental design. The first factor was concentration of sucrose ( 1%, 3%, 5%, 7%) and the second factor was concentration of yeast extract (0 mg/l, 100 mg/l, 200 mg/l, 300 mg/l). HPLC was used. To analyse quantitatively the artemisinin production, analysis of variance (ANOVA) and DMRT were used to analyse the data. The results showed that the addition of yeast extract and sucrose increased the concentration of artemisinin from the A. cina shoot culture. The highest artemisinin content produced was 14.035 mg/g dry weights which was produced by combination of 3 % sucrose concentration and 200 mg/l yeast extract. This result suggest that A. cina shoot culture could be used as a method to producing artemisinin.Key words : Artemisinin, shoot culture, Artemia cina Berg. Ex Poljakov

ANTIBACTERIAL ASSAY OF KAYU CENDANA (SANTALUM ALBUM L.) EXTRACT Partomuan Simanjuntak
Indonesian Journal of Pharmacy Vol 14 No 2, 2003
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

Identification of antibacterial compounds from cendana wood extract against Salmonella typhimurium, Escherichia coli, Kleibsiella sp. and Staphylococcus aureus had been conducted. The result showed that a and b-santalol was active against S. typhimurium and S. aureus where as epi-b-santalene is active against S. typhimurium.Key words : Santalum album; Santalaceae; Salmonella typhimurium; Escherichia coli; Klebsiella sp.; Staphylococcus aureus; a, b-santalol; epi-b-santalene

EFFECT OF RIFAMPICIN PRETREATMENT ON HIPOGLYCEMIC EFFECT OF GLYPIZIDE AMONG HEALTHY VOLUNTEERS Luciana Kuswibawati
Indonesian Journal of Pharmacy Vol 14 No 2, 2003
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

The incidence of tuberculosis in diabetic patients is high, therefore, the combination usage of antituberculosis (rifampicin) and antidiabetic (glypizide) medicines is inevitable. Rifampicin, an enzyme inductor, capable to influence the metabolism of other medicine when administered in concordance. The aimed of this study, therefore, was to investigate the influence of rifampicin pre-treatment on hypoglycaemic effect of glypizide (a second generation of sulphonylurea) among 12 Indonesia healthy volunteers, of both sexes. This study applied randomized crossover design receiving with and without rifampicin pre-treatment. Prior to starting the experiment, the pre-treatment group was given 450 mg of rifampicin orally daily for 7 days. Subsequently, single dose of 5 mg glypizide was administered to both of control and pre-treatment groups. The blood samples were then collected at a certain interval time for 7 hours. Glucose Oxydase (GOD) method were used to analyze the level of glucose in blood samples. The result showed no significant influence of rifampicin on hypoglycaemic effect of glypizide. However, it was found that rifampicin reduced significantly blood glucose level at 2.5 and 3 hours for 51.38 % and 20.58 %, respectively. The conclution of this study exhibit that pre-treatment with 450 mg of rifampicin daily for 7 days did not affect AUC0-7 blood glucose level as a result of single dose administration of 5 mg glypizide.Key words : rifampicin, glypizide, hypoglycaemic effect

IDENTIFICATION OF IROMP PROTEIN OF K.PNEUMONIAE RESISTANT MUTANTS AGAINST BRL41897 USING SDS –PAGE Kuswandi, M.
Indonesian Journal of Pharmacy Vol 14 No 2, 2003
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

Initial characterization, the MIC, Fe uptake and detection of siderophores, of the resistance K.pneumoniae mutants against BRL41897A (KSL mutants) showed that there were differences amongs them. Therefore it was necessary to observe the OMP and IROMP proteins of the mutants especially which had different MIC’s to the WT galur (M10). Results from SDS-PAGE analysis showed that KSL19 produced 49kDa protein weakly and similar result was found in the 22 kDa protein production by KSL38, KSL52, KSL58 and KSL59. Using Fe-CAA media -media lack of Fe - showed that 3 mutants synthesized certain protein weaker than M10 galur, KSL19 in the production of the 88 kDa protein, KSL38 and KSL59 at the 80 kDa protein. We also observed that KSL19 synthesized new 88 kDa protein. This result showed that certain mutant which had decreased production of one protein could stimulate another weak protein.Key words: SDS-PAGE – IROMP – K.pneumoniae mutants – BRL 41897A.

PURIFICATION OF RIBOSOME-INACTIVATING PROTEIN (RIP) OF MIRABILIS JALAPA L. LEAVES BY CM-SEPHAROSE CL-6B AND SEPHACRYL S-300HR COLUMN Sudjadi .; Sismindari .; Tenti Herawati; Alberta Tri Prasetyowati
Indonesian Journal of Pharmacy Vol 14 No 2, 2003
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

Total protein of Mirabilis jalapa leaves has activities to cleave supercoiled DNA to nick circular and linear and to cleave glycosidic bound of adenin4324 of yeast 26S rRNA. The protein was cytotoxic on HeLa and Raji celllines through apoptosis and necrosis mechanisms, respectively. However, the protein, poseess the activities, has yet been resolved. Therefore, protein furification to obtain single protein is necessary. Crude extract of M.jalapa leaves was prepared using 5 mM sodium phosphate buffer pH 7,2 containing 0,14 M sodium chloride. Total protein was obtained by precipitating the extract at 100% saturated ammonium sulfate and then dialyzed against phosphate buffer. The protein was purified by CM-Sepharose CL-6B, a cation exchange column. After loading the protein, the column was washed with 5 mM sodium phosphate buffer pH 6,5. The proteins were then eluted with linear gradient of increasing sodium chloride concentration. The fraction with supercoiled DNA-cutting activity was performed for N-glycosidase activity. The active fraction was a subject for further purification with Sephacryl S-300HR, a gel filtration column. The purity and size protein were confirmed by SDS-polyacrylamide gel electrophoresis with silver nitrate staining. RIP like protein was eluted from CM-Sepharose CL-6B on 0,25 – 0,3 M sodium chloride. The size of protein is around 30 kD.Key words : RIP purification, M.jalapa leaves, CM-Sepharose CL-6B, Sephacryl S-300HR

LABELING OF MIBI (METOXY ISOBUTYL ISONITRYL) WITH TECHNETIUM-99m AS A PERFUSION MYOCARDIAC IMAGING RADIOPHARMACEUTICAL Nurlaila Z.
Indonesian Journal of Pharmacy Vol 14 No 2, 2003
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

Coronary artery disease is one the major causes of death in Indonesia. For early diagnosis of the disease, it is deemed necessary to visualize the myocardium blood flow perfusion. 99mTc-MIBI labeled compound is one of the agent that could be used for such purpose. The labeling of MIBI with technetium-99m under some variations of several parameters has been carried out. The labeling efficiency was determined by counting the radiochemical purity using ITLC-SG 60 thin layer chromatography using two kinds of solvents, i.e. methanol and 0.9% NaCl solutions. The optimum labeling condition was achieved at the pH = 5.5 – 6.0, 1 mg of [Cu(MIBI)4BF4, 0.060 mg SnCl2.2H2O and 10 minutes incubation time in a boiling water bath, gave ± 99% of labeling efficiency. Under the formulation stated above, MIBI dry kit produces could give the radiochemical purity of more than 95%.Key words : metoxy isobutyl isonitril (MIBI), technetium-99m, labeling, myocardiac perfusion imaging.

BIOTRANSFORMATION OF PENTAGAMAVUNON-0 (PGV-O) : IN VITRO AND IN VIVO STUDIES ., Sugiyanto; ., Oetari; Nugroho, Agung Endro
Indonesian Journal of Pharmacy Vol 14 No 2, 2003
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

Pentagamavunon-0 (PGV-0) or 2,5-bis-(4-hydroxy-3-methoxy benzyilidin) cyclopentanon is a synthetic compound which has been proved to have an anti-inflamatory effect by animal experiments. The study was performed in order to investigate the possible route of biotransformation of PGV-0, which in turn can assist to elucidate the mechanism of its action.The in vivo biotransformation was predicted by identification of all metabolites of PGV-0 presence in urine and faeces after administration orally and intravenously. The in vitro studies were performed using microsomal or sitosolic fraction of the hepar. The metabolites were determined by thin layer chromatography and spectrophotometry.The results indicated that PGV-0 underwent oxydative biotransformation (phase I) and at least produced one metabolite. In vitro studies, PGV-0 also underwent glucuronidation and sulfatation. However, in vivo studies indicated that only metabolites of PGV-0 which have hydroxyl groups underwent conjugation process but PGV-0.Key words: Pentagamavunon-0, biotransformation, in vivo and in vitro.

THE EFFECT OF GINGER RHIZOME JUICE ON ACUTE TOXICITY OF PROPANOLOL AND QUINIDINE IN MICE Purwantiningsih .; Lukman Hakim
Indonesian Journal of Pharmacy Vol 14 No 2, 2003
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

The rhizome of ginger (Zingiber officinale, Rosc.) has been consumed by most people, as food ingredient or a traditional drug. In the previous research there was evidence that the ginger juice influenced the pharmacokinetics of propranolol (representative of drugs with high extraction ratio) and of sulfamezathine (as a model of sulfonamide drugs). The aim of this research is to study the effect of juice of ginger on the acute toxicity of propranolol and quinidine (a drug with a narrow therapeutic range). To achieve the aim of this study, mice were divided randomly into two groups, propranolol and quinidine. The each group was divided subsequently into controlled and pretreated groups. Mice in the controlled groups were given propranolol (I: 69 mg/kg BW; II; 82,80 mg/kg BW; III: 99,36 mg/kg BW; IV: 119,23 mg/kg BW) or quinidine (I: 265 mg/kg BW; II; 344,50 mg/kg BW; III: 447,85 mg/kg BW; IV: 582,25 mg/kg BW) through intraperitoneal injection, and those in pretreated group were pretreated with the ginger juice orally (5,6 ml/kg BW) an hour prior administration of propranolol or quinidine. The observations of the effect were done during the first three hours. The mice were killed and the vital organs were taken for histopathologic examination. The values of LD-50 were computed by Miller-Tainter Method (propranolol; LD-50controlled = 89,98 mg/kg BB dan LD-50treated = 87,51 mg/kg BB] [quinidine; LD-50controlled = 418,48 mg/kg BB dan LD-50 treated =331,93 mg/kg BB]. The results of this research showed that the ginger juice influences the acute toxicity of quinidine but the acute toxicity of propranolol.Key words: ginger rhizome, acute toxicity, mice, quinide, propanolol

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