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INDONESIA
METAMORFOSA Journal of Biological Sciences
Published by Universitas Udayana
ISSN : 23025697     EISSN : 26558122     DOI : -
Core Subject : Science, Agriculture, Social,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science Immunology & microbiology
METAMORFOSA is an electronic scientific journal published periodically by the Master of Biology Udayana University, which includes scientific works in the field of Biology.
Arjuna Subject : -
Articles 8 Documents
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Search results for , issue "Vol 3 No 1 (2016)" : 8 Documents clear
UJI DAYA HAMBAT Streptomyces roseoflavus AL2 TERHADAP Xanthomonas sp. PENYEBAB PENYAKIT HAWAR DAUN BAKTERI (HDB) PADA TANAMAN PADI (Oryza sativa L.) Ni Luh Cipta Ayumi Nellawati; Retno Kawuri; Ni Luh Arpiwi
Metamorfosa: Journal of Biological Sciences Vol 3 No 1 (2016)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2016.v03.i01.p01

Abstract

The purpose of this study was to determine the potential of Streptomyces in inhibiting the growth of pathogenic bacteria causing bacterial leaf blight (BLB) in vitro and identify the most likely type of Streptomyces that inhibits the growth of pathogens in the HDB. Bacterium Xanthomonas sp. used in this study were isolated from sick rice plants in Ayunan Village, Badung, Bali while Streptomyces isolates were isolated from the rhizosphere of reed plants (AL2), lily (LL1 and LL2), Aloe Vera (AV) and Elephant Grass (RG) from 3 different locations, namely PT. Alove Gianyar, Bedugul Botanical Garden and Buruan Village, Gianyar. Test results of antagonist test using dual culture method at the PCA medium showed that Streptomyces sp. AL2 has the most percentage of inhibition compared with other Streptomyces sp. isolates against pathogenic bacteria., namely 17.8 mm. The results of identification using the book Guide to the Classification and Identification of the Actinomycetes and Their Antibiotics (Waksman and Lechevalier, 1973) showed that the Streptomyces sp. AL2 is Streptomyces roseoflavus. The results provide an early indication that the filtrate of Streptomyces AL2. has the potential to be developed as a biocontrol of biological bactericide.
UJI POTENSI UMBI KIMPUL (Xanthosoma sagittifolium (L.) Schott.) SEBAGAI BAHAN PANGAN FUNGSIONAL ANTIULSER PADA MENCIT (Mus musculus L.) Triyani Yuliastuti; Marti Harini; Noor Soesanti Handajani; Tetri Widiyani
Metamorfosa: Journal of Biological Sciences Vol 3 No 1 (2016)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2016.v03.i01.p06

Abstract

Cocoyam (Xanthosoma sagittifolium (L.) Schott.) tuber is usually consumed as carbohydrates source. Because of highly mineral and fiber contents, it is potential to develop as a functional food source. The mineral contents are potassium, phosphor, magnesium, iron, copper and sodium. Some minerals are well known able to neutralize pH of the gastric fluid. It is necessary to study cocoyam activity on the ulcer gastric. The aims of this study were to determine anti-ulcerogenic activity of cocoyam tuber by observing macroscopic stomach mucous structure on mice and measuring the gastric fluid pH. Twenty male mice aged 2-3 months were used in this study. They were classified in to 4 different treatment groups. Group I was control mice without gastric ulcer, group II, III, and IV were mice with gastric ulcer by using aspirin treatment. Group II,III, and IV were treated with different treatments then for 7 days. Group II was treated by aquadest (negative control group), group III by sucralfat (positive control group), and group IV by cocoyam tuber. In the last treatment day, mice were fasted for 24 hours and then sacrified to pick stomach up. Gastric fluid was collected and the pH was measured then. Stomach mucous structure was observed macroscopically. Data in the gastric fluid pH was analyzed by using one-way ANOVA (Analysis of Variance) and continued  HSD (Honest Significant Difference)-Tukey test in 5% significance degree. Degree of stomach mucous structure damage was analyzed descriptively and then it was scored based on number and diameter size of ulcers. Data on the ulcer score were analyzed by using Kruskal-Wallis test. The results showed that there was a significant difference (p<0.05) at the stomach fluid pH. Normal group was not significantly different to the sucralfat and cocoyam tuber group, nevertheless normal group was different significantly to the ulcer group. Based on the degree of stomach mucous structure damage, there was not significantly different (p>0,05) between cocoyam tuber group and aquadest group. Nevertheless, mice in the cocoyam tuber group had lower ulcer score than mice in the aquadest group. Therefore cocoyam tuber is safe consumed by the gastric ulcer mice. Cocoyam tuber is potential to develop as a functional food on gastric ulcer patient.
STUDI LINGKAR TUMBUH POHON DI KAWASAN HUTAN TAMAN NASIONAL SIBERUT KEPULAUAN MENTAWAI . Mansyurdin; Tesri Maideliza; . Chairul; Ema Susiana
Metamorfosa: Journal of Biological Sciences Vol 3 No 1 (2016)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2016.v03.i01.p02

Abstract

Growth ring trees are formed by activity of the cambium which is influenced by the changing seasons.   In the tropical are not all of  trees species produce  the growth ring, because the season of  tropics is more uniform throughout the year and does not show sharp distinction between the periods of high rainfall and period of low rainfall. This study has concentred on several tree forest areas in Siberut National Park, Mentawai Islands. Samples were ollected by using borer on the main stem on the height of 130 cm. To see or not to see growing circle with to be checked macroscopically and microscopically. The macroscopic examination was done polished core by several grades of sandpaper in the transverse surface Tree species which have growth ring continued to microscopic observation with making slice anatomy. Based on 46 species of trees were examined, and  6 species were with found a growth ring in the number of cell mixture early wood and late wood from these species.
PENURUNAN FUNGSI HATI TIKUS BETINA (Rattus norvegivus L) YANG DIINJEKSI WHITE VITAMIN C DOSIS TINGGI DALAM JANGKA WAKTU LAMA DITINJAU DARI KADAR SGPT, SGOT SERTA GAMBARAN HISTOLOGI HATI Ni Wayan Sudatri; Iriani Setyawati; Ni Made Suartini; Dwi Ariani Yulihastuti
Metamorfosa: Journal of Biological Sciences Vol 3 No 1 (2016)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2016.v03.i01.p07

Abstract

This study aims to determine the side effects of high-dose injections of vitamin C in a long term against liver histology. This study used a completely randomized design (CRD) with different long treatments of vitamin C injection in various doses of P0 (control), P1 (30 days injection), P2 (50 days injection), P3 (70 days injection) and P4 (90 days injection). Parameters measured were plasma levels of SGPT and SGOT, the percentage of hydropic degeneration,  fatty degeneration, pyknotik nuclei, and necrosis of liver cells, and also inflammatory cell infiltration and sinusoid congestion. SGOT levels showed a significant differences (P=0,009) between the treatment and control group by ANOVA test. The Kruskal Wallis test showed that ALT levels were not significantly different (P=0,86), however, the levels of creatinine showed significant differences (P=0,002). The hydropic degeneration were not significantly different (P=0,054) by Kruskal Wallis test, whereas the fatty degeneration were significantly different (P=0,000) between the treatment and control groups by ANOVA test. Pyknotik nuclei and necrotic cells showed significant differences between the control and treatment with the P value of 0,016 and 0,01 respetively. The injected white vitamin C in high doses for long periods decreased the physiological functions of female mice liver.
STRUKTUR KERAPATAN VEGETASI DAN ESTIMASI KANDUNGAN KARBON PADA BEBERAPA KONDISI HUTAN DI PULAU SIBERUT SUMATERA BARAT . Chairul; Erizal Muchktar; . Mansyurdin; Tesri Maideliza; Gusmardi Indra
Metamorfosa: Journal of Biological Sciences Vol 3 No 1 (2016)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2016.v03.i01.p03

Abstract

Hutan Tropik memiliki sumber cadangan karbon sangat besar yang dapat berubah akibat perubahan kondisi hutan yang disebabkan oleh aktivitas  manusia, termasuk penebangan dan perkebunan sehingga mengancam perubahan iklim dunia. Kajian analisis dilakukan untuk menetahu struktur vegetasi dan kandungan karbon pada tiga kondisi hutan di Pulau Siberut sebagai upaya mitigasi perubahan iklim. Untuk mendapatkan data biomassa bagian atas tanah dan serasah digunakan metode plot berpetak.  Hasil Penelitian didapatkan 38 spesies dan 122 individu tumbuhan pada hutan primer, 22 spesies dan 49 individu pada hutan bekas tebangan serta 45 spesies dan 120 individu pada hutan tanaman campuran. Kandungan karbon bagian atas tumbuhan hidup pada hutan primer 1.359.884,68 kg/ha, hutan bekas tebangan 610.429,67 kg/ha  dan hutan tanaman campuran 360.793,70 kg/ha.  Kandungan karbon pada serasah hutan primer 774,49 kg/ha, hutan bekas tebangan 521,36 kg/ha dan hutan tanaman campuran 766,20 kg/ha.
PERANAN DEKOMPOSER NABATI TERHADAP PERTUMBUHAN POPULASI SEL ALGA Mochammad Junus; Agung Sugeng Widodo; Wahyono Suprapto; Windi Zamrudy
Metamorfosa: Journal of Biological Sciences Vol 3 No 1 (2016)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2016.v03.i01.p08

Abstract

Indonesia has programmed units of biogas as an energy producer. By-products such as organic sludge. Liquid separation of organic sludge biogas unit will be the terrestrial and aquatic plant fertilizer. Water plant that uses a liquid organic sludge biogas unit (LOSBGU) using vegetable decomposer were expected to be a very good influence on the increase of alga cells. The results showed that the addition of vegetable LOSBGU and decomposers in the media for 12 days alga could produce alga cells very significantly, however, its interaction was not significant. It was concluded that the addition of vegetable LOSBGU and decomposers in alga was very good media to enhance cell growth and its multiplication was also quite high. As suggestion, the use of  LOSBGU is needed to increase the production of alga.
ISOLASI DAN IDENTIFIKASI JAMUR Colletotrichum spp. ISOLAT PCS PENYEBAB PENYAKIT ANTRAKNOSA PADA BUAH CABAI BESAR (Capsicum annuum L.) DI BALI Sang Ketut Sudirga
Metamorfosa: Journal of Biological Sciences Vol 3 No 1 (2016)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2016.v03.i01.p04

Abstract

A research on the isolation and identification of fungi Colletotrichum spp. PCS isolate as the cause of anthracnose desease on chili fruit (Capsicum annuum L.) in Bali. Fungi Colletotrichum spp. isolated from large chilies anthracnose symptoms were taken from three locations of chili planting in Bali i.e. Apuan Village, Tabanan (isolates APN), Village Belok Sidan, Badung (isolates BLS) and Village Pancasari, Buleleng (isolates PCS). To ensure that these three isolates of the fungus caused anthracnose on chili fruit, tested Koch's postulates and virulence test were used. Based on fungal virulence test, isolates PCS was the most virulent isolates than APN and BLS isolates, therefore the PCS isolates was further identified. Macroscopic  and microscopic identification of the fungus Colletotrichum spp. isolate PCS were conducted at the Laboratory of Biopesticides, Faculty of Agriculture, University of Udayana by observing the shape of the colony, colony color, growth rate, hyphae structure, spore shape, and size of the spores. The identification of the fungus Colletotrichum spp. isolate PCS was carried out in the Department of Molecular Biology, Faculty of Mathematics and Natural Sciences, Bogor Agricultural University based on the analysis of 18S rRNA genes by using ITS1 and ITS4 primers. Phylogeny analyzes were performed using the MEGA 6.0, maximum parsimony method (MP) with 1000x bootstrap. The results showed that the fungus Colletotrichum spp. isolate PCS was a fungal of Colletotrichum acutatum as a clade with sequences of fungus C. acutatum by 100% of Bootstrap Support (BS). 
POTENSI Salmonella typhi YANG DILEMAHKAN DENGAN SINAR ULTRAVIOLET SEBAGAI VAKSIN ALTERNATIF Andreas Putro Ragil Santoso; Retno Kawuri; I Nengah Kerta Besung
Metamorfosa: Journal of Biological Sciences Vol 3 No 1 (2016)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2016.v03.i01.p05

Abstract

Salmonella typhi is a Gram-negative intracellular bacterium and causes typhoid fever in humans. The success rate of Berma Vivotif Ty21a vaccine in Indonesia is only 33-66%, while in other countries have been reached up to 100%. The research was conducted in order to determine the potency of local isolate bacteria to stimulate the immune response and the impact of different exposure frequencies on the immune response and the different immune response time when administered by UV-inactivated vaccine.. The results showed that the antibody titer of local isolates irradiated by UV light 10x was 88.76 ± 33.06 IU/mL at week 4 with the lowest antibody titer values about 11.15 ± 9.18 IU/mL was found in the negative control.

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