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INDONESIA
METAMORFOSA Journal of Biological Sciences
Published by Universitas Udayana
ISSN : 23025697     EISSN : 26558122     DOI : -
Core Subject : Science, Agriculture, Social,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science Immunology & microbiology
METAMORFOSA is an electronic scientific journal published periodically by the Master of Biology Udayana University, which includes scientific works in the field of Biology.
Arjuna Subject : -
Articles 21 Documents
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Search results for , issue "Vol 5 No 2 (2018)" : 21 Documents clear
Toksisitas dan Repelensi Minyak Sereh Dapur [Cymbopogon flexuosus (Nees ex Steud.) W. Watson] terhadap Kecoak Jerman (Blattella germanica L.) Syafri Yana; Resti Rahayu; Mairawita Mairawita
Metamorfosa: Journal of Biological Sciences Vol 5 No 2 (2018)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2018.v05.i02.p13

Abstract

The purposes of this study are to find out the toxicity and repellency of lemongrass oil (Cymbopogon flexuosus) to the German cockroach (Blatella germanica). Study was conducted from August to October 2016 in Research Laboratories of Animal Physiology, Department of Biology, Faculty of Mathematics and Natural Sciences Andalas University Padang. This study used the glass jar method (contact) with three strains of German cockroaches (VCRU-WHO, KRS-BDG and HHB-JKT) and lemongrass oil. The average of lethal time after 6 hour exposure ware counted with a probit analysis until found value LC90. The results showed that based on value LC90 strain VCRU-WHO 15,20%, strain KRS-BDG 22,42% and strain HHB-JKT 19,28% after 6 hour exposure.
Optimasi Periode Kultur Vibrio cholerae Ogawa pada Medium BHIB untuk Meningkatkan Daya Simpan Kultur Suta Arta; Retno Kawuri; Ida Bagus Gde Darmayasa
Metamorfosa: Journal of Biological Sciences Vol 5 No 2 (2018)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2018.v05.i02.p04

Abstract

Balai Besar Pengawas Obat dan Makanan (BBPOM) berkewajiban untuk menguji setiap produk pangan dari cemaran bakteri patogen sebelum diedarkan untuk mencegah terjadinya wabah penyakit yang ditularkan lewat makanan termasuk salah satunya Vibrio cholerae.Dalam proses pengujian produk pangan terhadap cemaran V. cholerae, terdapat masalah dalam hal ketahanan hidup kultur kerja V. cholerae.Penelitian ini merupakan penelitian eksperimental laboratorik dengan menggunakan Rancangan Acak Lengkap(RAL) Pola Faktorial.Tujuan dari penelitian ini untuk mendapatkan waktu pertumbuhan maksimal kultur V. cholerae Ogawa pada media (Brain Heart Infusion Broth)BHIB pada suhu 37oC serta untuk mendapatkan kombinasi derajat keasaman (pH) dan konsentrasiNaCl yang tepat pada medium BHIB untuk meningkatkan umur kultur kerja V. cholerae Ogawa yang disimpan pada suhu kulkas (4-8oC). Penelitian diawali dengan re-identifikasi isolat kemudian dilakukan uji kurva tumbuh dengan inkubasi 37oC selama 15 jam pada medium BHIB. Setelah diperoleh pola pertumbuhannya pada medium BHIB, penelitian dilanjutkan dengan menguji ketahanan V. cholerae Ogawa pada medium BHIB yang dimodifikasi dengan kombinasi pH (5,6,7,8, dan 9) dan konsentrasi NaCl (0,1,2, dan 3%) pada penyimpanan suhu kulkas (4-8oC). Hasil penelitian uji kurva tumbuh pada medium BHIB menunjukkan bahwa fase log (pertumbuhan maksimal) terjadi pada jam ke-5. Hasil uji ketahanan pada medium BHIB yang dimodifikasi dengan kombinasi pH dan konsentrasi NaCl menunjukkan bahwa kombinasi pH 9 dan NaCl 3% merupakan kombinasi terbaik untuk meningkatkan umur daya simpan V. cholerae Ogawa pada medium BHIB. Pada kombinasi tersebut V. cholerae Ogawa mampu bertahan sampai hari ke-51 pada penyimpanan suhu kulkas (4-8oC).
Analisis Kandungan Vitamin C Buah Naga Merah (Hylocereus polyrhizuz) dan Buah Naga Putih (Hylocereus undatus) pada Penyimpanan dengan Suhu dan Waktu yang Berbeda N. Putri Sumaryani; Anak Agung Istri Mirah Dharmadewi
Metamorfosa: Journal of Biological Sciences Vol 5 No 2 (2018)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2018.v05.i02.p18

Abstract

This study was conducted to determine the temperature and storage time of the exact vitamin C content of red and white dragon fruit and the difference of vitamin C. The samples were taken from the plantation land in Ubud Andong Highway, Petulu, Gianyar Regency. Treated for 10 and 30 days with 2, 10, 20 and 300C temperature treatment with five repetitions using Completely Randomized Design Factorial pattern. Data obtained statistically using ANOVA (Analysis of Variance). The highest vitamin C content in red dragon fruit is 29.61 mg/ 100g temperature 100C for 10 days and the lowest is in 30 days storage with temperature 300C that is equal to 22,09 mg/ 100g. While the white dragon fruit content of vitamin C highest contained in storage with a temperature of 20C is 22.69 mg/ 100g with maximum storage of 10 days and the lowest at a temperature of 300C is 17.37 mg/ 100g.
Kemampuan Antagonis Cendawan Endofit terhadap Rhizoctonia sp. Penyebab Penyakit Busuk Akar Tanaman Sorgum (Sorghum bicolor L.) secara In Vitro Aloysius Rusae; Bernadina Metboki; Blasius Atini
Metamorfosa: Journal of Biological Sciences Vol 5 No 2 (2018)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2018.v05.i02.p09

Abstract

Sorgum (Sorgum bicolor L.) is a serelia plant which is potential to becultivated and grown in marginal areas. The advantages of sorghum lie in its extensive agroecological adaptability and resistance to drought. This commodity has a high nutrient content, so it is very good to be used as a source of food and alternative animal feed. Rhizoctonia sp. The cause of root rot is one of the important pathogens in sorghum plants. These pathogens can survive in the soil and can also survive in seeds. Microorganism that developed symbiosis with Zorgum is useful for growth stimulation and biocontrol. The objectives of the researcha re toidentify endofit mushroom that developed symbiosis on some varieties of zorgum and to make an antagonis test on Rhizoctonia sp, the cause of spoiled root disease. This research is conducted in several streas: firstly, take the sample of same varieties of zorgum lokal merah,lokal putih,and numbu in Naiola in Kecamatan Bikomi Selatan while the isolation and identification are held in Faculty of Agrociture of University of Timor and secendly, do antagonist test on Rhizoctonia sp. The study on lokal merah variety has found 7 izolats: Aspergilus sp 1, Aspergilus sp 2, Aspergilus sp 3, Fusarium sp 1, Fusarium sp 2, Trichoderma sp 2. Mushroom endofits found in lokal putih variety are Aspergilus sp.2, Aspergilus sp.3, Fusarium sp.1, dan Trichoderma sp.2. Mushrooms on numbu variety are Fusarium sp. 2, Aspergilus sp.2 dan Trichoderma sp.1. Endofit Mushroom from theroot ofzorgum has 3 genus Aspergilus sp, Fusarium sp dan Trichoderma sp. Trichoderma sp 1 has 20,1 % antagonist character.
Keberadaan Mikroba Pemfermentasi pada Minuman Kefir Air Susu Kambing Etawa Merla Pamericar; Periadnadi Periadnadi; Nurmiati Nurmiati
Metamorfosa: Journal of Biological Sciences Vol 5 No 2 (2018)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2018.v05.i02.p15

Abstract

Study about existence of fermentation microbe in kefir etawa goat milk had been studied. The objective of this study were to know total of microbe from kefir etawa goat milk. This study used descriptive method and counting population of microbe on in vitro. The variable acid in kefir measuring with pH meter. Result indicated total bacteria in kefir etawa goat milk are sebanyak 98,3 x 106 cfu/ml with total yeast 67,8 x 105 cfu/ml and pH of kefir 4.57.
Aktivitas Antimikroba Ekstrak Segar Tumbuhan Sikaduduak (Melastoma malabathricum Linn.) Nissa Arifa; Periadnadi Periadnadi
Metamorfosa: Journal of Biological Sciences Vol 5 No 2 (2018)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2018.v05.i02.p05

Abstract

Penelitian tentang “Aktifitas Antimikroba Ekstrak Segar Tumbuhan Sikaduduak (Melastoma malabathricum Linn.)” menggunakan Rancangan Acak Lengkap dengan pola Nested. Penelitian ini bertujuan untuk mengetahui aktivitas antimikroba ekstrak segar buah masak, daun dan bunga Sikaduduak. Hasil penelitian menunjukkan bahwa ekstrak segar daun memiliki daya hambat terbesar terhadap Staphylococcus aureus dan Escherichia coli dengan rata-rata diameter zona hambat yaitu 17,33 mm dan 14,67 mm. Terhadap Candida albicans rata-rata diameter zona hambat terbesar (11,67 mm) terdapat pada buah masak.
Gambaran Histopatologi Otot Korpus Kavernosum Tikus Wistar (Rattus norvegicus) Hiperlipidemia Anak Agung Ayu Putri Permatasari; Ni Kadek Yunita Sari; I Gede Widhiantara
Metamorfosa: Journal of Biological Sciences Vol 5 No 2 (2018)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2018.v05.i02.p19

Abstract

This study aims to see the histopathology of muscle corvus cavernosum penis wistar rats (Rattus novergicus) hyperlipidemia seen in terms of thickness and picture of tissue abnormalities. Hyperlipidemia is a risk factor for erectile dysfunction ateriosklerosis in men. This research is an experimental research with one group prettest - posttest design. Samples in this study were 10 adult male aged 3-4 months wistar rats with a weight range 150-200 grams. Prior to treatment, surgery was performed for histopathology of 5-tailed rat samples at pretest. After that, sample were made with hyperhidemia by feeding fat for 50 days. Then performed surgery for histopathological preparation of corvus cavernosum muscle in posttest. The results showed that the thickness of the corvus cavernosum of the penile mouse of hyperlipidemia decreased significantly (P?0,05) when compared with the pretest group. In the histopathologic picture occurs muscle atrophy and the existence of abnormal structures of fibrotic and collagen tissue.
Identifikasi Fungi Endofit dari Rimpang Lengkuas (Alpinia galanga L.) sebagai Antifungi terhadap Candida albicans Ni Made Raningsih; Putu Wahyu Sri Juniantari Sandy
Metamorfosa: Journal of Biological Sciences Vol 5 No 2 (2018)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2018.v05.i02.p10

Abstract

The main objectives of this research were to isolat and identify endophytic fungi antagonists of Candida albicnas from galanga rhizomes. Samples of galanga rhizome were collected from Gitgit plantation in Gitgit village, Buleleng regency. Isolation and identification were conducted in Food Microbiology Laboratory, Faculty of Agriculture, Udayana University. There were three isolates identified with inhibitory persentage againts Candida albicans i.e. Trichoderma viridae (26.07±3.76%), Trichoderma harzianum (45.11±5.84%) and Fusarium oxyforum (20.11±5.61%).
Struktur Komunitas Fitoplankton di Area Keramba Jaring Apung Danau Buyan Kecamatan Sukasada, Buleleng, Propinsi Bali Putu Roni Graha Persada; I Wayan Restu; Alfi Hermawati Waskita Sari
Metamorfosa: Journal of Biological Sciences Vol 5 No 2 (2018)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2018.v05.i02.p03

Abstract

Danau Buyan merupakan sumberdaya alam akuatik yang mempunyai nilai yang sangat penting. Pemanfaatan danau buyan diantaranya adalah kegiatan budidaya keramba jaring apung yang berpotensi memberikan masukan nutrien kondisi ini dapat mempengaruhi kualitas air yang kemudian akan berpengaruh pada fitoplankton. Tujuan penelitian ini adalah untuk mengetahui struktur komunitas fitoplankton dan kualitas perairan di area keramba jaring apung Danau Buyan, Kecamatan Sukasada, Propinsi Bali. Penelitian ini dilaksanakan pada bulan Januari-Maret 2016 di area keramba jaring apung Danau Buyan, Kecamatan Sukasada, Kabupaten Buleleng, Propinsi Bali. Penelitian ini menggunakan metode sistematic sampling. Lokasi stasiun pengamatan total berjumlah 8 stasiun, stasiun 1-7 terletak dekat dengan area budidaya keramba jaring apung sedangkan 1 stasiun kontrol berada jauh dari area keramba jaring apung. Kegiatan pengambilan sampel dilakukan 3 kali dengan selang waktu 3 minggu. Hasil penelitian menunjukkan terdapat 9 genus fitoplankton yang terdiri dari 24 spesies. Hasil kelimpahan fitoplankton rata-rata yaitu 1454 sel/l sehingga masuk dalam kategori perairan oligotropik, Indeks keanekaragaman 07647-1,8352, Indeks keseragaman rata-rata-rata 3937. Nilai indeks dominansi 0,4225. Nilai parameter kualitas air yang didapatkan antara lain yaitu nilai suhu sebesar 23,54-24,94oC, Nilai Kecerahan yaitu 66,41-251.06, Nilai kekeruhan yaitu sebesar 2,10-6,39 NTU, Nilai pH yaitu sebesar 7,56-8,93, Nilai oksigen terlarut (DO) yaitu sebesar 4,10-5,44 mg/l, Nilai nitrat yaitu sebesar 0,39-0,99 mg/l dan nilai fosfat yaitu sebesar 0,13-0,45 mg/l. Secara keseluruhan hanya parameter oksigen terlarut (DO) yang kurang mendukung kehidupan fitoplankton.
Hambatan in Vitro Cendawan Antagonis pada Fusarium sp., Penyebab Penyakit pada Tanaman Buah Naga (Hylocereus undatus (Haw.) Britton & Rose) Dewa Ayu Andriastini; Yan Ramona; Meitini Wahyuni Proborini
Metamorfosa: Journal of Biological Sciences Vol 5 No 2 (2018)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2018.v05.i02.p14

Abstract

A research on in vitro inhibition of fungal antagonists, isolated from dragon fruit plantation in Sembung village, Bali, on Fusarium sp. (the disease causative agent of dragon fruit plant) was conducted with the main objective to investigate the effectiveness of these fungal antagonists to inhibit the in vitro growth of the pathogen. Dual assay method was applied in this experiment. The results showed that three potential fungal antagonists were successfully isolated in this research and they were identified as Trichoderma harzianum, Aspergillus niger, dan Paecilomyces lilacinus. All these fungal antagonists showed antagonistic activity against Fusarium sp. which was statistically significant (p<0.05) when compared to control. This indicated that all antagonist isolates were potential to be developed as biocontrol agent candidates.

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