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INDONESIA
METAMORFOSA Journal of Biological Sciences
Published by Universitas Udayana
ISSN : 23025697     EISSN : 26558122     DOI : -
Core Subject : Science, Agriculture, Social,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science Immunology & microbiology
METAMORFOSA is an electronic scientific journal published periodically by the Master of Biology Udayana University, which includes scientific works in the field of Biology.
Arjuna Subject : -
Articles 20 Documents
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Search results for , issue "Vol 6 No 2 (2019)" : 20 Documents clear
Induksi Akar dan Pertumbuhan Stek Pucuk Jirak (Eurya acuminata DC.) dengan Pemberian Beberapa Zat Pengatur Tumbuh Pada Berbagai Media Tanam Tressa Pratywi Gupitasari; Zozy Aneloi Noli; Suwirmen Suwirmen
Metamorfosa: Journal of Biological Sciences Vol 6 No 2 (2019)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2019.v06.i02.p19

Abstract

The research about roots induction and growth of shoot cutting of Jirak (Eurya acuminata DC.) by adding some growth regulator solutions in various planting medium, was conducted from May until August 2017 in Greenhouse and Plant Physiology Laboratory, Department of Biology, Faculty of Mathematics and Natural Science, Andalas University. The aims of the research to find out effect of providing growth regulator solution with different types, used of planting medium and interaction of both factors the best to roots induction and growth Jirak (E. acuminata DC.) by cutting. The research used Complete Randomized Design (CRD) factorial with two factors and three replications. Factors A were growth regulator solution (a0: control, a1: IBA 100 ppm, a2: NAA 100 ppm, a3: IAA 100 ppm) and factors B were various planting medium (b0: garden soil, b1: sand, b2: charcoal husk). The research result were adding some growth regulator solutions and various planting medium significanly affected roots induction and growth of jirak and there is interaction on the high of plant. The treatment of 100 ppm IAA and treatment using sand medium obtain the highest result in increasing roots induction and growth of shoot cutting of Jirak.
Produksi Virgin Coconut Oil (VCO) yang Dikatalis oleh Lactobacillus plantarum dan Enzim yang Terkandung dalam Sari Nanas Helda Thorikul Affan; Yan Ramona; Ni Luh Suriani
Metamorfosa: Journal of Biological Sciences Vol 6 No 2 (2019)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2019.v06.i02.p03

Abstract

Indonesia is a tropical country with an area of coconut plantation of 3.7 Ha. The fruits of this plant have been used as materials in the production of Virgin Coconut Oil (VCO). Such oil is known to contain higher Medium Chain Fatty Acid (MCFA) or Medium Chain Triglycerides (MCTs) than original frying oil. The MCTs of this oil is very useful to avoid obesity, to reduce total cholesterol as well as to function as anti-microbes. These have been considered as a cheap solution to solve health related problems. The main objective of this research was to produce VCO catalyzed by pure culture of Lactobacillus plantarum isolated from a commercial VCO (Vici’O) combined with enzymes extracted from pineapple fruits. The study was conducted at the laboratory of Microbiology, Faculty of Mathematics and Sciences, Udayana University. The study was started with isolation and identification of Lactobacillus sp. from a commercial VCO. The VCO production method combined the two starters of Lactobacillus isolates (at concentrations of 0 to 10% v/v) and pineapple juice with various volumes (0 to 60 mL). In the first stage of the project, 3 potential Lactobacilli isolates (Sp1, Sp2, and Sp3) were successfully isolated and were confirmed to be Lactobacillus sp. In the main experiment, combination of 10% v/v Lactobacillus starter and 60 mL pineapple juice produced the highest VCO yield (43,17% v/v) with improved organoleptic properties, lower water content, lower saphonification, and lower peroxide than that specified by SNI No 7381: 2008.
Pengaruh Pemberian Pakan Alami Kopepoda Jenis Acartia sp. Terhadap Pertumbuhan Nener Bandeng (Chanos chanos) Dionisius Darmawan Sutanto; Deny Suhernawan Yusup; Joko Wiryatno
Metamorfosa: Journal of Biological Sciences Vol 6 No 2 (2019)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2019.v06.i02.p15

Abstract

Copepods is a zooplankton which is common to be found in the seas and one of the primary food of fish larvae. Copepods believed to be one of the key of aquaculture development because it has higher nutritional value. One of the most researched copepods species is Acartia sp. because having shorter life cycle and the most easily found compared to other copepods species. However information about copepods growth still limited. The research of copepods species Acartia sp. growth was aimed to collect information about Acartia sp. body length and width so that it can known whether fish larvae mouth opening match with copepods body size. This research was done in March 2017 at Gondol Reseach Institute of Mariculture, Gondol, Bali. Acartia sp. sample was collected from mass culture pond’s outlet pipe as much as 250 mL with three repetitions so the total sample was 750 mL then around 1 mL was taken to be observed in the microscope dissecting set which equipped with micrometer at 64 times magnification for measurement process. Variable to be observed was Acartia sp. body length and width. From the research it was known Acartia sp. has four phase of life cycle which is egg, nauplii, copepodit, and adult. Acartia sp. eggs have a measurement around 0,04 mm to 0,05 mm, then nauplii sized around 0,069 mm to 0,182 mm, while copepodit sized around 0,363 mm to 0,584 mm, and the adults sized around 0,865 mm. Acartia sp. has a rapid population growth because one adult Acartia sp. can produce 16 eggs.
Identifikasi Senyawa Aktif Ekstrak Daun Tembelekan (Lantana camaraL.) Yang Berpotensi Sebagai Pengendali JamurFusarium solani (Mart.) Sacc. Penyebab Layu Batang dan Busuk Akar Tanaman Pisang Kepok(Musa paradisiacaforma typica) Ni Made Dewi Wahyuni; Ni Putu Adriani Astiti; Meitini Wahyuni Proborini
Metamorfosa: Journal of Biological Sciences Vol 6 No 2 (2019)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2019.v06.i02.p08

Abstract

Banana plants have been used as parts of ritualism by Hindu’S people in Bali nevertheles these plants are often infected by fungal pathogens. Fusarium sp is one of the most common fungal pathogen infecting these plants. Commonly to release this pathogen the usage synthetic fungicides. To reduce the use of these synthetic fungicides, alternative method such as using natural compound of fungicides has been researched. The main objective of this research was to investigate the compounds of tembelekan leaf extract to control the growth of F. solani (Mart.) Sacc, the causative agent of stem wilt and root rot in kapok banana by using GC-MS. The result of identification using GC-MS identified that there are four compounds is 2,2,4-Trimethyl-1,3-pentanediol diisobutyrate, Hexadecanoic acid, Terephthali acid, and Squalene.
Potensi Pakih Sipasan (Blechnum orientale) sebagai Antibakteri Terhadap Staphylococcus aureus dan Methicillin Resistant Staphylococcus aureus Yossi Rahmadeni; Fuji Astuti Febria; Amri Bakhtiar
Metamorfosa: Journal of Biological Sciences Vol 6 No 2 (2019)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2019.v06.i02.p12

Abstract

Staphylococcus aureus is an opportunistic pathogenic bacteria. These bacteria can cause infections of the skin, bones, lungs, heart or systemic infections. Giving antibiotics does not fit the recommendations result in the emergence of resistant strains. Methicillin Resistant Staphylococcus aureus (MRSA) is one of the bacteria beta-lactam antibiotic resistant. Pakih Sipasan (Blechnum orientale) is a low level plants that is widely used Simanau villagers at Solok District in overcoming skin infections. The aims of this research are to determine the phytochemical content of B. orientale and to know antibacterial potential against S. aureus and MRSA. This research was conducted in March-July 2017 at Biota Laboratory of Sumatera and Microbiology Research Laboratory of Biology Department, Andalas University. Research method is experiment. The extracts were prepared with concentrations of 20 mg/ml, 40 mg/ml, 60 mg/ml, 80 mg/ml and 100 mg/ml. The data obtained are analyzed and presented in the form of tables and drawings are presented descriptively. The results showed that the B. orientale contain chemical compounds class of alkaloids, phenols and flavonoids. All treatments have potential as antibacterial to S. aureus and MRSA. The antibacterial activity of the B. orientale against S. aureus is stronger than against MRSA. The average diameter of inhibitory zone formed from concentrations of 20 mg/ml to 100 mg/ml of S. aureus ranged from 13.00 mm - 14.95 mm and to against MRSA between 11.31 mm - 14.48 mm. Key words: Staphylococcus aureus, MRSA, phytocemical test, antibacteria, Blechnum orientale
Aspek Biologi Dan Hubungan Kekerabatan Padi Lokal (Oryza sativa L.) Di Desa Wongaya Gede Kecamatan Penebel, Kabupaten Tabanan, Bali Gusti Ayu Nyoman Budiwati; Eniek Kriswiyanti; Ida Ayu Astarini
Metamorfosa: Journal of Biological Sciences Vol 6 No 2 (2019)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2019.v06.i02.p20

Abstract

The aim of this research was to determine morphological characteristics, number of local rice varieties, family relationship and type of pests and weeds that disrupt the life cycle of local rice varieties in Wongaya Gede Village, Penebel, Tabanan Regency, Bali. Research was conducted in three Subak (Piak, Keloncing and Bedugul) located in Wongaya Gede Village; and Plant Structure and Development Laboratory, Department of Biology, Faculty of Mathematics and Natural Sciences, Udayana University, August 2015 - January 2016. Field observation include morphological characteristics from seedlings to harvest phase (85 characters), identification of pests and weeds as well as environmental factors that affect local rice growth. Pollen viability was examined using 1% aniline blue in laktofenol. Result of the study revealed that local rice characteristics in Wongaya Gede Village can be observed based on morphological stems, leaves, inflorescentia, grain, rice, agronomic and anatomical, shape type and pollen viability and endosperm types. Six varieties of local rice in Wongaya Gede Village are Merah Cendana, Injin, Putih Cempaka, Ketan Beton, Ketan Tahun, Jaka Selem and one varieties of ‘unggul’ rice is Mansur. Fenogram shows local rice varieties were divided into two groups, namely Indica (Mansur and Ketan Beton) and Javanica (Merah Cendana, Injin, Putih Cempaka, Ketan Tahun and Jaka Selem). Weeds that disrupt the life cycle of local rice in Wongaya Gede Village can be divided into two groups; sedges and broad leaves. Pests observed were Mamalia (mammals), Aves (birds) and Insecta (insects). Keyword: Fenogram, Local knowledge, Morphological relationship, Pollen, Subak
Diversitas Serangga Hutan Tanah Gambut Di Palangkaraya Kalimantan Tengah Julian Tambunan; I Ketut Ginantra; Ni Luh Watiniasih
Metamorfosa: Journal of Biological Sciences Vol 6 No 2 (2019)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2019.v06.i02.p04

Abstract

This study aims to determine the diversity of insects and plants used as a habitat in unburned and previously burned peat land forests in Palangkaraya, Central Kalimantan. Insects were collected by beating the branches of plants, aerial sweeping, pitfall traps and light traps. Plants were identified in situ or plant samples were collected and identified later in the Lab. The diversity of insects were compared between unburned and previously burned peat forests by analyzing its index diversity (H’) and index of similarity (IS). In total, the insects collected from unburned peat forest were 551 individual, belongs to 12 order and 51 families, and 431 individual insects were collected from previously burned peat forest which belongs to 10 order and 38 families. The family of insects that most frequently found at both areas was Formicidae (Hymenoptera). The insects diversity of both forests were still high, that is H' = 3,45 of unburned peat forest and H '= 3,11 of previously burned peat forest, with the similarity index IS > 50% of both peat forests. The number of plant species found was higher in unburned peat forest (38 species) than in previously burned peat forest (9 species). The previously burned forest was dominated by Acacia plants, while in unburned peat forest the plants seem to evenly spread.
BEBERAPA ASPEK EKOLOGI IKAN KULARI (Lobocheilos falcifer C.V : Cyprinidae) DI SUNGAI BATANG KURANJI PADANG, SUMATERA BARAT Yusrika Ika Kadifi; Indra Junaidi Zakaria; Efrizal Efrizal
Metamorfosa: Journal of Biological Sciences Vol 6 No 2 (2019)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2019.v06.i02.p16

Abstract

This research was carried out.in January-June 2013. This research aimed to analyze some ecological aspects (habitat, body length, distribution, growth pattern) in Kulari fish (Lobocheilos falcifer C.V : Cyprinidae) at Batang Kuranji river, Padang. This studied use survey method with stratified random sampling technique (at three locations) to collected samples. The result of this study showed that water temperature has 26,550C; pH 5,6; O2 5,78 mg/l; BOD5 0,79mg/l; CO2 1,81 mg/l; TSS 0, 51; depth 40.88 cm, and current velocity 0.50 m/s. The growth patterns of kulari fish showed that male fishes were in negative allometric and female fishes were in isometric. Keyword: Ecology aspects, Kulari fish (Lobocheilos falcifer), Batang Kuranji River
PERILAKU BURUNG PECUK PADI BELANG (Phalacrocorax melanoleucos) DI PULAU SERANGAN, BALI Putu Ayu Wiwin Vitrayanthi; Ni Luh Watiniasih; I Ketut Ginantra
Metamorfosa: Journal of Biological Sciences Vol 6 No 2 (2019)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2019.v05.i02.p09

Abstract

Serangan island is part of Ngurah Rai Forest Park. Fifty five species of birds, belong to 30 families have been found in Serangan island. Based on earlier information that Phalacrocorax melanoleucos was commonly shown in lagoon and mangrove. This study aimed to investigate the habitat condition and the behavior of P. melanoleucos in Serangan Island. The study has been conducted from November 2016 to May 2017, in lagoon area of Serangan Island in two observation points of 8º44.647? S 115º13.332? E and 8º44.769? S 115º345? E. Scan sampling technique was applied to collect the data of the bird behavior and the data of species of plant being used by P. Melanoleucos was collected by tracking methods. P. melanoleucos was commonly found foraging in the morning (45 % ± 0.15 %). Similar pattern of behavior was observed during the day and in the afternoon, but the amount of time spent for perching during the day and afternoon were slighly lower, 42 % ± 0.02 % and 41 % ± 0.02 % respectively. Agonistic behaviour was rarely observed during the study (5 % ± 0.06 %). Casuarina equisetifolia and Soneratia alba plants were commonly used by birds for perching. Key words : Serangan island, habitat, Scan sampling, Phalacrocorac melanoleucos, perching,
Keragaman Genetik dan Hubungan Kekerabatan Ayam Lokal Berdasarkan Penanda RAPD Mery Wintari; Made Pharmawati; Ngurah Intan Wiratmini
Metamorfosa: Journal of Biological Sciences Vol 6 No 2 (2019)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2019.v06.i02.p13

Abstract

Indonesian local chicken is one kind of germplasm that has been utilized by the community for various needs, such as consumption, ceremonial, collection, and cockfighting. To maintain its sustainability, an inventory of genetic diversity can be made using molecular markers. This study aimed to determine the genetic diversity and relationship between four Indonesian local chickens: ayam kampung, ayam ketawa, ayam kate lokal, and ayam bekisar kangean, based on RAPD marker. DNA was extracted from blood samples of 12 local chickens consisting of 3 ayam kampung, 3 ayam ketawa, 3 ayam kate lokal, and 3 ayam bekisar kangean using Genetic DNA Mini Kit (Tissue) Geneaid. The PCR-RAPD analysis was performed using 6 primers, OPA 04, OPB 08, OPB 12, OPC 16, OPH 01, and OPH 03. The product of PCR-RAPD was electrophoresed using 1,5% agarose in 1x TAE buffer. The results showed that the similarity value of 12 local chickens using RAPD ranged from 0% to 100%. The relationship between 12 local chickens using RAPD marker indicated that the chickens in the same morphology were not clustered in the same group. This suggests that the same group of chickens phenotypically does not necessarily have the same genotype. Keywords: Local Chicken, PCR-RAPD

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