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INDONESIA
METAMORFOSA Journal of Biological Sciences
Published by Universitas Udayana
ISSN : 23025697     EISSN : 26558122     DOI : -
Core Subject : Science, Agriculture, Social,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science Immunology & microbiology
METAMORFOSA is an electronic scientific journal published periodically by the Master of Biology Udayana University, which includes scientific works in the field of Biology.
Arjuna Subject : -
Articles 20 Documents
 1   2 
Search results for , issue "Vol 8 No 2 (2021)" : 20 Documents clear
Kadar Superoksida Dismutase (SOD ) Pada Paru-Paru Tikus Hipertensi Doca-Garam Yang Dipapar Ekstrak Metanolik Benalu Teh Dan Benalu Mangga Siti Qurrotul Aini; Nour Athiroh Abdoes Sjakoer; Nurul Jadid Mubarakati
Metamorfosa: Journal of Biological Sciences Vol 8 No 2 (2021)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2021.v08.i02.p13

Abstract

Hypertension is a condition caused by the pressure of blood against the artery walls when the blood is Hypertension is a condition caused by the pressure of blood against the artery walls when the blood is pumped from the heart to the tissues. In hypertension, it can stimulate the formation of reactive oxygen species (ROS) compounds. The use of exogenous antioxidants in preventing oxidative stress can alternatively use herbal plants. For example, herbal plants such as tea parasites and mango parasites can increase the activity of superoxide dismutase (SOD) and reduce the concentration of malondialdehyde (MDA). This study aims to further examine the role of methanolic extracts from the combination of tea parasite and mango parasite (EMBTBM) on superoxide dismutase (SOD) in hypertensive rats exposed to DOCA-salt using experimental methods. Using 25 male Wistar rats, consisted of 5 treatments, namely control (-), control (+), the treatment group for the combination of tea parasites and mango parasites with doses of 50, 100, and 200 mg / KgBB respectively with five repetitions. The results of this study indicate that the level of increased SOD with the combination of EMBTBM in the control group (+) was very significantly different from the control group (-), treatment 1, 2, and 3. This very significant difference was due to the p-value = <0.001 (< 0.05). The benefit of this study is that it can be used as a reference for the role of the combination of tea parasites and mango parasites on superoxide dismutase (SOD) in hypertensive rats exposed to DOCA-salt. Keywords: Hypertension, EMBTBM, SOD.
The Role of City Forest: Study of Malang Urban Forest Contribution in Sequestrating Carbondioxide Emition Roimil Latifa; Diani Fatmawati; Ahmad Fauzi
Metamorfosa: Journal of Biological Sciences Vol 8 No 2 (2021)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2021.v08.i02.p03

Abstract

Malabar, as an urban forest has a potential in CO2 sequestration which was not documented yet. This quantitative descriptive research aimed to describe the contribution of Malabar urban forest in CO2 emission mitigation through calculating the amount of CO2 absorbed by the forest. The data collection used was non-destructive in which the indicators measured were DBH and the height of the trees with DBH more than 20 cm. The data gained then was analyzed using Alometric Chave formula to obtain the amount of plant biomass, carbon values, and CO2 values. The results showed that the potential of Malabar rban forest is not optimized yet as the low amount of carbon stored in this forest. Thus, it is suggested to enrich the trees planted with those which can sequestrate CO2 in much higher amount compare to the exist one. Kata kunci: CO2 sequestration, urban forest, Malabar urban forest
Penilaian Kualitas Air Hulu Sungai Bedadung Kabupaten Jember Berdasarkan Trophic Diatom Index Retno Wimbaningrum; Ahmad Farid Ary Wardhana; Hari Sulistiyowati
Metamorfosa: Journal of Biological Sciences Vol 8 No 2 (2021)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2021.v08.i02.p19

Abstract

This study was conducted to assess the quality of the Bedadung river water that passes through forests, coffee plantations, rice fields and land-settlements with epilitic diatom bioindicators based on the Trophic Diatom Index value. Diatom samples were collected by brushing the surface of the rock submerged in river water, then the samples were identified and their abundance was determined. The physico-chemical parameters of river water that are measured include temperature, TP, DO, TDS, conductivity, turbidity and water discharge. The quality of land around the river is also determined by first recording the growing vegetation data. Diatom data were analyzed to determine the TDI index value to determine river water quality, vegetation data were analyzed to determine the index of environmental services. Furthermore, TDI data, environmental service index and physico-chemical parameters of river water are analyzed. Principle Component Analysis to determine the relationship between these components. The results showed that the water quality of the Bedadung river that passed through forests, coffee plantations, rice fields and land-settlements based on the TDI value was classified into the moderate eutrophic category because the TDI value ranged from 41-47. This TDI value is related to TP levels, water discharge and DO but not related to land quality around rivers, pH, TDS, conductivity, and temperature. Keyword: TDI, epilithic diatom, river water quality, bioindicator
Uji Isolat Bakteri Selulolitik Sebagai Dekomposer Pada Dekomposisi Tandan Kosong Kelapa Sawit Clara Aprilya Kurniawan; Gusmawartati gusmawartati
Metamorfosa: Journal of Biological Sciences Vol 8 No 2 (2021)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2021.v08.i02.p08

Abstract

Tandan kosong kelapa sawit (TKKS) merupakan salah satu jenis limbah padat terbesar yang dihasilkan oleh pabrik kelapa sawit (PKS). Setiap pengolahan 1 ton tandan buah segar (TBS) menghasilkan 220–230 kg TKKS. Salah satu upaya mengatasi penumpukan TKKS adalah pengomposan. Kendala utama pengomposan TKKS adalah proses perombakannya memerlukan waktu 6?12 bulan dikarenakan TKKS mengandung selulosa 33,02%, hemiselulosa 22,05% dan lignin 35,08%. Salah satu cara mempercepat proses pengomposan dilakukan melalui perlakuan biologi dengan menambahkan mikroorganisme seperti bakteri selulolitik yang mampu menghasilkan enzim selulase. Penelitian dilaksanakan secara eksperimen menggunakan rancangan acak lengkap (RAL) non faktorial yang terdiri dari delapan perlakuan yaitu B0 (Tanpa pemberian isolat), B1 (Bacillus tequilensis Strain RA 1402), B2 (Bacillus subtilis Strain C17), B3 (Bacillus subtilis Strain SKUASIS), B4 (Bacillus subtilis Strain DSM 10), B5 (Bacillus subtilis Strain K43), B6 (Pseudomonas aeruginosa Strain KUJM) dan B7 (Gabungan Isolat). Parameter yang diamati adalah sifat fisik kompos, penyusutan berat bahan kompos dan total populasi mikrob kompos. Hasil penelitian menunjukkan bahwa pemberian isolat bakteri selulolitik memberikan pengaruh nyata terhadap penyusutan berat bahan kompos. Perlakuan gabungan isolat (B7) merupakan perlakuan terbaik dalam meningkatkan penyusutan berat bahan kompos. Perlakuan B7 merupakan perlakuan yang mampu menghasilkan kompos berkualitas tinggi dimana telah sesuai dengan Standar Kualitas Kompos SNI : 19?7030?2004. Kata kunci: Tandan kosong kelapa sawit, Isolat bakteri selulolitik, Pengomposan,
Peranan BAP dan Air Kelapa pada Medium VW terhadap Organogenesis Dendrobium sp. Nindi Afifa Nisa; Tintrim Rahayu; Gatra Ervi Jayanti
Metamorfosa: Journal of Biological Sciences Vol 8 No 2 (2021)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2021.v08.i02.p14

Abstract

Anggrek Dendrobium merupakan genus favorit bagi pecinta anggrek di bandingkan dengan anggrek lainnya, karena kemampuan anggrek Dendrobium yang mudah beradaptasi di berbagai lingkungan. Produksi anggrek di Indonesia sangat lambat, sedangkan peminatnya dari tahun ke tahun semakin meningkat, sehingga perlu di percepat dengan kultur jaringan secara in vitro. Perbanyakan tanaman secara in vitro ini menggunakan media VW sebagai faktor penentu, media VW adalah media yang sering digunakan pada perbanyakan tanaman Dendrobium sp. Tujuan Penelitian ini adalah untuk mengetahui peranan BAP dan air kelapa pada organonogenesis eksplan Dendrobium sp. Penelitian ini di dilakukan di Laboratorium Kultur Jaringan FMIPA, Universitas Islam Malang, mulai dari bulan September sampai November 2020, menggunakan metode eksperimen dengan Rancangan Acak Lengkap (RAL). Penelitian ini terdiri dari 2 perlakuan dengan 3 ulangan, BAP 0,5 ml, 1 ml dan 1,5 ml dan air kelapa100 ml, 150 ml dan 200 ml. Hasil penelitian menunjukkan bahwa organogenesis tertinggi pada perlakuan BAP yaitu konsentrasi 1,5 ml dengan rata-rata 1,3 tunas, 3,86 daun, 0,86 akar dan panjang akar 0,4 cm. Sedangkan perlakuan air kelapa menunjukkan organogenesis tertinggi yaitu konsentrasi 150 ml, dengan rata-rata 0,63 tunas, 3,53 daun, 1 akar dan panjang akar 1,66 cm. Perlakuan air kelapa lebih efektif pada pemanjangan akar dengan jumlah akar 1 dan panjang akar 1,66 cm. Sedangkan perlakuan BAP lebih efektif pada pembentukan tunas dengan jumlah tunas 1,3 dan jumlah daun 3,86. Kata kunci: BAP, Bahan organik, Dendrobium, Organogenesis.
Umur, Lingkungan Pengendapan, dan Karakteristik Fosil Palinomorf Formasi Batuasih, Sukabumi, Jawa Barat Rizki Satria Rachman; Winantris Winantris; Budi Muljana
Metamorfosa: Journal of Biological Sciences Vol 8 No 2 (2021)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2021.v08.i02.p04

Abstract

Palynomorph is a dust-sized material that is resistant to strong acids which can reflect the age and environmental conditions when a rock was formed. Batuasih Formation is one of the oldest rock formations in Bogor Basin which is rarely interpreted, especially from its palynological aspect. This study aims to see palynomorph characteristics, relative age, and environmental conditions in Batuasih Formation. There are several steps in conducting the research. Field activities by measured stratigraphic sections and sampling every 5-meter intervals or each lithological changes, laboratory activities by sample preparation using acid treatment method, identification were carried out using CX-22 binocular microscope, and laboratory analysis of age and depositional environment is drawn based on palynomorph associations that are present in rocks. The result found that palynomorphs were present in 8 of 10 samples (B.1, B.2, B.4, B.6 - B.10). One hundred seventy-three palynomorphs identified to consist of 20 different species. Dominant pollen came from genus Proxapertites, both Proxapertites operculatus, Proxapertites cursus, and Proxapertites psilatus. While dominant spores came from Verrucatosporites usmensis. Apart from pollen and spores, Palynomorphs in the form of dinoflagellates and foraminifera test lining are also present in this formation. Palynomorph analysis shows that Batuasih Formation has Late Eocene - Early Oligocene age (41.2 - 27.8 Ma) with transitional depositional environment.
Profil Histopatologi Otot Jantung pada Tikus Hipertensi (Doca-Garam) yang Dipapar Kombinasi Ekstrak Metanolik Benalu Teh Dan Benalu Mangga (EMBTBM) Annisatul Anis Hakimah; Nour Athiroh Abdoes Sjakoer; Nurul Jadid Mubarakati
Metamorfosa: Journal of Biological Sciences Vol 8 No 2 (2021)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2021.v08.i02.p20

Abstract

Some herbs from the parasite group and the Loranthaceae family also act as antihypertensives. This study aims to look at the histopathological profile of the heart in hypertensive rats (DOCA-Salt) exposed to the combination of Methanolic Extract of Benalu Tea and Mango Benalu (EMBTBM) for 14 days using true experimental design method with completely randomized design on 50 rats, divided into 5 controls -), control (+), treatment 1 dose 50 mg / KgBB, treatment 2 doses 100 mg / KgBB and treatment 3 doses 200 mg / KgBB. Each treatment had 5 replications. In analyzing the data used the ANOVA test, one of the methods is JAMOVI with version 1.1.9.0. As for the calculation of cell diameter using the IMAGE J application. The results showed that the histopathological profile of the heart in hypertensive rats (DOCA-Salt) exposed to EMBTBM for 14 days on heart cell necrosis showed very real evidence between the control (+) and control (- ), treatment 1, 2, and 3. This is evidenced by the results of the analysis p-value <0.05, namely 0.002, so it is denied that EMBTBM has an effect on the average number of necrosis of cardiac cells. There is also a significant difference in the number of heart cell diameter of hypertensive rats between the control (+) and control (-) groups, treatment 1, 2, and 3. This is evidenced by the results of the analysis showing a p-value <0.05, namely 0.021, so that it can it is denied that EMBTBM can shrink the diameter of cells in the heart organ. Keywords: Hypertension, Heart, Left Ventricle, Anatomical Histopathology, EMBTBM.
The Isolasi Aktinomiset Pelarut Fosfat Asal Perakaran Tembakau (Nicotiana tabacum L.) di Antirogo Jember Esti Utarti; Saniyah Fatkhul Alim; Dwi Setyati; S Sutoyo
Metamorfosa: Journal of Biological Sciences Vol 8 No 2 (2021)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2021.v08.i02.p09

Abstract

Fosfor (P) merupakan makronutrien terpenting kedua yang dibutuhkan tanaman. Fosfat dalam tanah berada dalam bentuk terikat oleh ion Fe3+, Al3+ dan Ca2+ sehingga tidak tersedia bagi tanaman. Penelitian ini bertujuan untuk mengisolasi dan mendapatkan aktinomiset sebagai agen pelarut fosfat dari perakaran tembakau (Nicotiana tabacum L.). Jenis penelitian ini termasuk dalam penelitian eksplorasi yang dilanjutkan dengan pengamatan morfologi secara makroskopis dan mikroskopis isolat pelarut fosfat terbaik. Sampel penelitian diambil dari Desa Antirogo, Kecamatan Sumbersari, Kabupaten Jember. Sampel tanah diambil dari bagian perakaran tanaman tembakau. Isolasi aktinomiset dilakukan dengan metode pengenceran dan disebar pada media SCA. Pemurnian dilakukan pada media ISP-2 atau ISP-4. Uji aktivitas pelarutan fosfat menggunakan media Pikovskaya dan diukur indeks pelarutan fosfatnya. Sebanyak 71 isolat aktinomiset berhasil diisolasi dari tanah perakaran tembakau di daerah Antirogo. Hasil skrining menunjukkan bahwa terdapat 28 isolat (39,4%) dari 71 isolat yang diperoleh, memiliki kemampuan melarutkan fosfat. Lima isolat tertinggi berdasarkan rangking indeks pelarut fosfat terbaik dilakukan karakterisasi morfologi makroskopis dan mikroskopis. Berdasarkan bentuk rantai spora, empat isolat aktinomiset yang memiliki rangking indeks pelarutan fosfat tertinggi termasuk dalam genus Streptomyces. Kata kunci: aktinomiset, pelarut fosfat, perakaran, Antirogo Jember
Keanekaragaman Jenis Tumbuhan Bahan Ramuan “Boreh Basanbuat” Untuk Memperlancar Produksi Air Susu Ibu (ASI) di Bali Enik Kriswiyanti; Ni Nyoman Darsini; Junita Hardini; Ni Putu Ariwathi
Metamorfosa: Journal of Biological Sciences Vol 8 No 2 (2021)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2021.v08.i02.p15

Abstract

Boreh (bahasa Bali) merupakan salah satu bentuk ramuan tradisional berupa campuran rempah-rempah yang dihaluskan, ditambah cairan (air/arak/minyak kelapa) kemudian dilulurkan pada bagian tubuh. Salah satu boreh yang dapat digunakan untuk memperlancar produksi air susu ibu (ASI) adalah boreh basanbuat. Penelitian ini bertujuan untuk mengetahui jenis dan bagian tumbuhan yang dapat digunakan untuk membuat boreh basanbuat di Bali. Metode penelitian yang digunakan: purposive sampling, pengumpulan data dengan cara wawancara, observasi dan dokumentasi dengan pedagang crakenan, pengobat tradisional (battra) dan masyarakat dari kota dan kabupaten wilayah propinsi Bali, sebanyak 90 responden. Hasil penelitian didapatkan 54 jenis tumbuhan (52 genus, 29 familia) untuk membuat 64 jenis ramuan, jenis tumbuhan yang paling banyak digunakan dari anggota famili Zingiberaceae, bagian tumbuhan yang paling banyak digunakan adalah daun (23,21%). Ramuan boreh basanbuat umumnya menggunakan 5-6 jenis tumbuhan, yaitu menggunakan: beras (Oryza sativa L.), biji kelabet (Trigonella feonum-graecum L.), akar wangi (Androphogon zizanioides (L) Urban), kayu/bubuk cendana (Santalum album L.), daun delem/ nilam (Pogostemon cablin (Blanco) Benth.) dan rimpang kencur (Kaemferia galanga L). Manfaat bahan boreh selain memperlancar produksi ASI, juga untuk aroma terapi, antiinflamasi, anti pembengkakan, antibakteri, antijamur, dan lain sebagainya.
Deteksi Keberadaan Coliform dan Escherichia Coli pada Es Batu dari Penjual Minuman di Sekitar Kampus 4 Universitas Ahmad Dahlan Oktira Roka Aji; Nofa Nur Fiani
Metamorfosa: Journal of Biological Sciences Vol 8 No 2 (2021)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2021.v08.i02.p05

Abstract

Ice cubes are a complement to drinks. Ice cubes must not contain Coliform or Escherichia coli. Coliform and Escherichia coli are indicator microorganisms used in water quality analysis. Its presence indicates fecal contamination. The purpose of this study was to analyze the presence of Coliform and E.coli on ice cubes found in drinks sold around the Kampus 4 Universitas Ahmad Dahlan. Sampling was done by purposive sampling. The sample used in this study was 7 ice cube samples taken from different stalls around the campus. This study used the MPN (Most Probable Number) method which consists of a presumptive test, a confirmative test and the Escherichia coli test. A positive result was indicated by changes in the turbidity of the media and the presence of gas bubbles in the durham tube. Data analysis was performed by comparing the number of positive tubes in the observation results with the MPN table. Based on the data obtained, samples of ice cubes from 7 sellers around Kampus 4 Universitas Ahmad Dahlan were positive for Coliform and Escherichia coli bacteria. The principles of food sanitation hygiene need to be improved to prevent bacterial contamination on ice cubes.

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