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INDONESIA
METAMORFOSA Journal of Biological Sciences
Published by Universitas Udayana
ISSN : 23025697     EISSN : 26558122     DOI : -
Core Subject : Science, Agriculture, Social,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science Immunology & microbiology
METAMORFOSA is an electronic scientific journal published periodically by the Master of Biology Udayana University, which includes scientific works in the field of Biology.
Arjuna Subject : -
Articles 20 Documents
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Search results for , issue "Vol 8 No 2 (2021)" : 20 Documents clear
Perkembangan Dan Ukuran Pertama Kali Matang Gonad (Lm50) Ikan Lemadang (Coryphaena hippurus Linnaeus, 1758) Betina Di Perairan Selatan Bali Hety Hartaty; Iriani Setyawati; FX. Sudaryanto
Metamorfosa: Journal of Biological Sciences Vol 8 No 2 (2021)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2021.v08.i02.p02

Abstract

Research on the reproductive biology of fish can provide information on spawning frequency, spawning success, spawning time, and size at first maturity. This research aimed to determine the reproductive biology of common dolphinfish (Coryphaena hippurus Linnaeus, 1758) landed at the fish landing site (PPI) Kedonganan, Badung, Bali. Sampling was conducted in May - September 2018. A total of 42 female common dolphinfish with length ranged between 41-110 cm FL were obtained from hand-line fishery based on FADs. Ovaries were prepared using the paraffin method and Harris Haematoxylin-Eosin staining for histological observation. The results showed that the gonad maturity level in adult females was: spawning (38.10%), spawning capable (35.71%), regressing-potentially reproductive (4.76%), regressed 1 (2.38%), regressed 2 (2.38%), and regenerating (2.38%). Immature female: immature (9.52%) and developing (4.76%). Oocyte diameter ranged between 43,20 – 864,66 µm with an average of 430,99 µm. Fecundity in fish size 49-75 cm FL ranged from 30,720-433,080 eggs / fish with an average of 129,594 eggs / fish. The difference in the level of oocytes in the ovaries at the same time showed that female common dolphinfish in the southern Bali waters has an asynchronous ovary development and indeterminant fecundity with multiple spawning strategies and size at first maturity (Lm50) was 43.2 cm FL. Keywords: common dolphinfish, gonad histology, size at maturity
Potential of Awar-awar Leaf Methanol Extract (Ficus septica Burm. f.) Against Aspergillus niger on Balinese Lontar Ni Komang Deny Julyeda; Ida Bagus Gde Darmayasa; Junita Hardini
Metamorfosa: Journal of Biological Sciences Vol 8 No 2 (2021)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2021.v08.i02.p10

Abstract

Ficus septica Burm. f. is a wild plant that has antifungal compounds. Exploration of antifungal compounds from natural material important in inhibiting the growth of Aspergillus niger, one of Balinese lontar destructive fungus. Natural material use to reduse lontar damage due to synthetic materials. This study aims to inhibition of awar-awar leaf methanol extract (in vitro). The method used was a complete randomized design with 7 treatments for the concentration of the awar-awar leaf methanolic extract of 10.000, 20.000, 30.000, 40.000, 50.000 ppm (b/v), positive control (nystatin), and negative control (methanol). The minimum inhibitory concentration (MIC) test uses 6 treatments for the concentration of the awar-awar leaf methanolic extract of 100, 500, 1000, 2000 ppm (w/v), positive control (nystatin), and negative control (methanol). Giving the extract to the diffusion well with potato dextrose agar (PDA) media. The research data were analyzed statistically ANOVA (DMRT with 5% significance level) with the SPSS 23 version for windows. The results showed that the awar-awar leaf methanol extract had MIC at a concentration of 500 ppm (w/v). Concentrations of 10.000-50.000 ppm (w/v) indicate the inhibition zone diameter of 20.50 mm, respectively; 23.00 mm; 23.50 mm; 23.75 mm; 24.75 mm. The concentration of 10.000 ppm (w/v) has higher inhibition than the positive control. The concentration of 20.000 ppm (w/v) was the optimum concentration of inhibition of awar-awar leaf methanol extract. Conclusion of this research was awar-awar leaf methanol extract had anti-fungal compounds which inhibited the growth of A. niger. This extract can be used as an alternative to inhibit the growth of fungi that cause lontar damage. Keyword: Aspergillus niger, destructive fungi, Ficus septica Burm. f.
Identifikasi Jamur Endofit pada Tanaman Anggur Bali (Vitis vinifera L. Var Alphonso lavalle) serta Potensi Antagonisnya terhadap Botrytis cinerea Pers. Penyebab Penyakit Busuk Kelabu Putu Anggan Pradipta Utama; Meitini Proborini Wahyuni; Ni Made Susun Parwanayoni
Metamorfosa: Journal of Biological Sciences Vol 8 No 2 (2021)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2021.v08.i02.p01

Abstract

This research aims to isolate and test the ability of endophytic fungi from the Bali Grape plant (Vitis vinifera L. var Alphonso lavalle) against botrytis cinerea pers. cause of gray rot in Grapes Bali. This research is an exploration study that is then continued with experimental. This research sample was isolated from Balinese grapes, namely healthy roots, stems, and leaves as well as diseased fruit parts from Balinese vineyards in Banyupoh Village, Gerokgak, Buleleng, Bali. Isolates of endophyte fungi that are successfully isolated are then tested using dual culture methods against pathogenic fungi. The test results of five isolates of endophytic fungi obtained three types of fungi that have the tasteless power of two types of fungi Trichoderma spp. (1) (2) and Mucor spp. with consecutive bonding power of 90.67 ± 16.17%, 75.67 ± 31.13%, and 82.67 ± 39.02. These results show that endophyte fungi have potential as biocontrol agents. Keywords : Mushroom endophyte, Botrytis cinerea Pers., Biocontrol
Tingkah Laku Bertelur dan karakteristik Fisik Sarang Maleo (Marcocephalon maleo) di Cagar Alam Panua Desa Maleo Kecamatan Paguat Febrianti Muhi; Dewi Wahyuni K. Baderan Baderan; Mustamin Ibrahim
Metamorfosa: Journal of Biological Sciences Vol 8 No 2 (2021)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2021.v08.i02.p17

Abstract

The study aimed to identify the egg-laying behavior and characteristic of the nest of Maleo birds. The survey study conducted first-hand direct observation based on the facts at the site. An explorative method was employed to acquire the data of nest characteristics. The method refers to the exploration of the laying nest and measurement of physical characteristics of the nest that contains the egg. The results revealed that the Maleo bird’s egg-laying behavior comprised: digging hole, tracing for any potential dangers, caching, looking for good, perching, finding a good location, roosting, playing, and dancing. The characteristics of laying nest of Maleo bird in the research site were as follows: 1) the average nest depth and width was 65 cm and 62 cm respectively; 2) the average temperature of laying nest hole from morning until afternoon was at 31.75-33.6ºC, while the average temperature of the nest surface was at 27.5-50.5ºC. Other environmental factors comprised: 20-35% humidity rate, acidity (pH) of seven, while the main constituents of nest were 98-100% sand, 0-2% dusts, and 0% clay. The bird’s dominant egg-laying behavior was digging hole. Keyword: characteristics, Marcocephalon maleo, behavior, nest
Studi Keragaman Anggrek Epifit di Kepulauan Sula Arief Widyantoro
Metamorfosa: Journal of Biological Sciences Vol 8 No 2 (2021)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2021.v08.i02.p06

Abstract

Kegiatan eksplorasi anggrek epifit saat ini sangat penting dilakukan karena rusaknya habitat alami disebabkan alih fungsi hutan menjadi lahan perkebunan. Anggrek epifit yang sifatnya menempel pada pohon tersebar di hutan produksi terbatas, hutan produksi tetap, dan hutan produksi dapat dikonversi. Namun, perluasan areal perkebunan kelapa, cengkeh dan kakao berdampak menyempitnya areal hutan sehingga berakibat berkurangnya habitat alami anggrek epifit. Penelitian bertujuan mempelajari jenis-jenis anggrek epifit di Kepulauan Sula, Provinsi Maluku Utara. Studi menggunakan metode survey dengan pendekatan deskriptif dan penentuan plot pengamatan. Hasil survey telah ditemukan 6 spesies anggrek epifit pada 8 plot antara lain Cymbidium finlaysonianum, Grammatophyllum scriptum, Trichoglottis latisepala, Brachypeza sp., Pomatocalpa spicata, dan Aerides sp. Anggrek epifit C. finlaysonianum mendominasi kawasan hutan mencapai 77,29%. Terdapat 9 pohon inang sebagai habitat alami anggrek yang ditemukan meliputi Shorea sp., Cythea sp., Mangifera indica, Heritiera sp., Thesphesia macrophylla, Artocarpus sp., Calophyllum inophyllum, Ficus sp dan Cocos nucifera. Kata kunci: anggrek epifit, pohon inang, keragaman, Kepulauan Sula
Inventarisasi Jenis Avifauna di Taman Wisata Alam Menipo Kabupaten Kupang Nardi Matias Leo; L.P.E.K Yuni; I Ketut Ginantra
Metamorfosa: Journal of Biological Sciences Vol 8 No 2 (2021)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2021.v08.i02.p16

Abstract

ABSTRACT Menipo Natural Park, Kupang Regency, East Nusa Tenggara is a popular tourist spot because it has various types of flora and fauna, one of which is avifauna. Avifauna is used as a good indicator to determine environmental health in the development of sustainability and the value of biodiversity as a whole. This study aims to determine the diversity of avifauna species that inhabit or utilize the Menipo Nature Tourism Park as their survival. The study was conducted for three months using the Point Count method. The number of counting points was adjusted to the area of ??the research area. The limit of the observation radius was 25 m from the standing observation point, the observation time at each point was calculated as 10 minutes, to record and to take pictures of the bird species. This study found that there were 36 species of avifauna from 25 families and had moderate species diversity, either in mangrove forest (2.86), coastal forest (2.66), and savana (2,28) which was carried out by calculating the diversity index. All bird species utilize all vegetation types for foraging, resting, and nesting. Keywords: Birds, bird diversity, Menipo Natural Park
Aktivitas Antioksidan Ekstrak Kulit Jeruk Keprok (Citrus reticulata Blanco) So’e Sebagai Teh Tradisional Deglory Tunmuni; Ni Putu Adriani Astiti; Sang Ketut Sudirga
Metamorfosa: Journal of Biological Sciences Vol 8 No 2 (2021)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2021.v08.i02.p11

Abstract

The Citrus So'e plant is one of the leading community plants of the people of East Nusa Tenggara (NTT). The So'e tangerine is used as a raw material in the manufacture of orange juice and the peel has not been used. This research was conducted to utilize So'e tangerine peel as a traditional tea. The parameters observed included the active compound group, antioxidant activity, the type of active compound for So'e tangerine peel and the public's perception of the traditional So'e tangerine peel tea. The research method was carried out by qualitative phytochemical tests, test for the content of flavonoids, phenolics, and tannins by spectaphotometry, antioxidant tests using the DPPH method, testing for the types of active compounds using GC-MS and organoleptic tests to determine the level of consumer preference. The results showed that So'e tangerine peel extract had active compounds, namely flavonoids, phenolics, tannins, steroids, triterpenoids and alkaloids and So'e tangerine peel extract contained flavonoids = 253.901 mg / 100g QE, phenolic = 61.6099 mg / 100g GAE, tannin = 60.0496 mg / 100g TAE which has the potential as an antioxidant with an IC50 value of 81.811 mg / L which is included in the strong antioxidant category, and has various types of active compounds, namely the nonpolar fraction contains 53 compounds and the polar fraction contains 46 compounds activity and the level of public preference for So'e tangerine peel tea was higher in refined tea than coarse tea. Keywords: Citrus reticulata, phytochemicals, DPPH, GC-MS, traditional tea.
Formulasi Serum Ekstrak Buah Malaka (Phyllanthus emblica) Sebagai Anti Aging Rosa Ichi Harum Pratiwi; ni luh arpiwi
Metamorfosa: Journal of Biological Sciences Vol 8 No 2 (2021)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2021.v08.i02.p12

Abstract

Serum merupakan produk cairan sedikit kental yang diaplikasikan pada kulit wajah. Penggunaan serum di kalangan wanita sudah banyak diminati bahkan menjadi salah satu perawatan rutin. Seiring berkembangnya zaman, kini banyak orang yang tidak bertanggungjawab dalam menjual produk serum. Kandungan serum diproduksi dengan bahan kimia yang tidak sesuai dosis atau pun menambahkan bahan aktif yang dapat memicu terjadinya masalah kulit. Beberapa masalah kulit yang timbul yaitu bintik-bintik hitam, garis-garis halus dan penuaan. Mengurangi dampak masalah kulit tersebut, maka dilakukan pembuatan serum berbasis “back to nature” yang mengutamakan bahan herbal sebagai salah satu alternatif formulasi serum yang aman. Salah satu bahan alternatif tersebut yaitu buah malaka (Phyllanthus emblica). Buah malaka dipercaya memiliki kandungan antioksidan yang tinggi sehingga dapat memperlambat penuaan dan dijadikan bahan serum untuk anti aging. Metode yang digunakan dalam penelitian yaitu dengan uji DPPH dan uji fitokimia. Hasil penelitian menunjukkan bahwa ekstrak buah malaka tergolong antioksidan kuat dengan nilai aktivitas antioksidan 143,557%, nilai IC50 sebesar 38,649 ppm, flavonoid sebesar 4,6123%, tanin sebesar 112,21%, vitamin C 27,0625%, aktivitas antioksidan serum F1 0,0008%, F2 0,0121% dan F3 0,0103%. Uji viskositas menunjukkan nilai 15.000 mPars, pH pada serum F1 yaitu 4,79; F2 5,00; F3 5,75. Uji homogenitas serum menunjukkan kehomogenan yang baik dan uji hedonik terhadap 3 sampel serum (F1, F2 dan F3) dengan 30 probandus. Hasil yang paling optimal yaitu serum F3 dengan konsentrasi 1,5 %.
Elusidasi Dan Daya Hambat Ekstrak Etanol Daun Sembung Delan (Sphaeranthus indicus L.) Terhadap Candida albicans ATCC 1023 Yulita Salma Lani; Ida Bagus Gde Darmayasa; Ni Made Susun Parwanayoni
Metamorfosa: Journal of Biological Sciences Vol 8 No 2 (2021)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2021.v08.i02.p18

Abstract

The sembung delan (Sphaeranthus indicus L.) plant is one of the weeds in Indonesia which has great potential as an alternative antifungal. The purpose of this study was to determine the ability of sembung delan leaf extract to inhibit the fungus Candida albicans ATCC 1023, as a pathogenic species and cause of candidiasis, as well as chemical compounds contained in the extract. The research design used in this study was a completely randomized design (CRD) divided into 7 treatments and 4 repetitions with one treatment factor, namely the concentration of 1% (w/v), 2% (w/v), 3% (w/v), 4% (w/v), 5% (w/v). Positive control (Nystatin) and negative control (Solvent). Phytochemical tests were carried out and analysis of compound content using GC-MS. The results showed that sembung delan leaf extract was able to inhibit C. albicans ATCC 1023 with the greatest inhibition, namely at a concentration of 5% (w/v) with a diameter of 13.00 mm, a minimum concentration of 0.3% (w/v) with a diameter of 5.925 mm. Statistically significantly different (P?0.05) with a concentration of 0.1% (w / v), and 0.2% (w / v) with a diameter of 0.000 mm and an optimum concentration of 4% (w/v) with a diameter of 11.25 mm. Phytochemical test results of the crude extract of sembung delan contain saponins, phenols, steroids, terpenoids, alkaloids, and tannins. For GC-MS analysis, 7 active compounds were obtained with 6 of them known as anti-fungal compounds, namely Thymol; 1-Tetradecanamine, N, N-dimethyl-; Benzene, 2-methoxy-1,2,3-trimethyl-; 6-Octenal, 3,7-dimethyl-; Thymol and Benzyl chloride. The active compounds contained in sembung delan leaf extract have the opportunity to be used as herbal ingredients to control C. albicans ATCC 1023. Keywords: Minimum Inhibitory Concentration, phytochemicals, thin layer chromatography, gas chromatography mass spectrometry.
Indeks Ekologi dan Status Konservasi Jenis Burung (Avifauna) di Desa Gunaksa dan Beberapa Wilayah di Sekitarnya di Kabupaten Klungkung, Bali I Made Sara Wijana; I Made Saka Wijaya; M. Rheza Rizki Syahputra; Gde Oka Widiyavedanta; Egmont congdenjit; Gede Surya Indrawan; Abd. Rahman As-syakur; Putu Angga Wiradana
Metamorfosa: Journal of Biological Sciences Vol 8 No 2 (2021)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2022.v08.i02.p07

Abstract

The Gunaksa Village and several surrounding areas in Klungkung Regency, Bali have the potential to be developed as one of the international ecotourism destinations in Indonesia. This study aims to determine the diversity and status of bird species (avifauna) in the Gunaksa Village Area and several surrounding areas so that it is expected to be the basis for information on natural resource management in this area. The method used is the "Cruise method", which is tracing paths that can be used to represent various types of ecosystems in the research area. The results showed that 46 species of birds were obtained with the highest Relative Abundance Index (RAI) value obtained by the buffalo egret (Bubulcus ibis) of 9.50% (dominant). The ecological index of bird species shows a high category in the Diversity Index (H') (3.16), Richness Index (R) (7.15), and Evenness Index (E) (1.87). Species of the black-winged kite, Javan ermine, Christmas frigatebird, and Pied fantail are protected species based on the Regulation of the Minister of Environment and Forestry of the Republic of Indonesia Number p.106/menlhk/setjen/kum.1/12/2018, while only the frigatebird Christmas is included in the IUCN RedList with the Critical (CR) category. Conservation efforts and the participation of government agencies, academics, practitioners and the community are needed to increase the environmental awareness of the Gunaksa area and its surroundings for the sustainability of Avifauna in the area.

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