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Buletin Veteriner Udayana
Published by Universitas Udayana
ISSN : 20852495     EISSN : 24772712     DOI : https://doi.org/10.24843/bulvet.
Core Subject : Health, Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Immunology & microbiology Medicine & Pharmacology Public Health Veterinary
The Buletin Veteriner is focused on Veterinary Medicine and Animal Sciences study with its various developments
Arjuna Subject : Kedokteran Hewan - Kedokteran Hewan (Lain-Lain)
Articles 34 Documents
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Search results for , issue "Vol. 15 No. 6 December 2023" : 34 Documents clear
Pengaruh Lama Simpan Semen Ayam Ekor Panjang dengan Pengencer Kuning Telur dan Air Kelapa Wulung pada Suhu 5oC Dyah Risma Aprilia Enji; Wayan Bebas; I Gusti Ngurah Bagus Trilaksana
Buletin Veteriner Udayana Vol. 15 No. 6 December 2023
Publisher : The Faculty of Veterinary Medicine, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2023.v15.i06.p19

Abstract

Long-tailed chickens or commonly called onagadori chickens are classified as rare and endangered animals. This ornamental chicken has the advantage of its long tail reaching 10 meters. This study aims to determine the effect of storing semen with egg yolk coconut water diluent stored at cold temperatures on motility, abnormalities and viability. The design in this study used a completely randomized design (CRD) with 7 treatment groups for storage duration, namely 0 hours, 12 hours, 24 hours, 26 hours, 48 hours, 60 hours, 72 hours. The research object used was male long-tailed chickens that were sexually mature at the age of 6-8 months. Chicken semen storage technique by massaging the chicken's back (massage). Semen examination was carried out macroscopically and microscopically. Viability and abnormality of spermatozoa were observed using the eosin negrosin staining method. This study concluded that the motility, viability and spermatozoa abnormalities of long-tailed chickens in egg yolk coconut water diluent could be maintained for 48 hours at 5°C storage temperature with 52.41% motility, 50.15% viability and 7.33% abnormality. In order to obtain optimal results in supporting the success of artificial insemination, long-tailed chicken semen with egg yolk diluent and coconut water should not be stored for more than 60 hours.
Gambaran Histopatologi Hepar Tikus Putih Penderita Fibrosarkoma Pasca Viroterapi Newcastle Disease Isolat Virulen Ni Luh Aricahyani; Anak Agung Ayu Mirah Adi; Ida Bagus Oka Winaya
Buletin Veteriner Udayana Vol. 15 No. 6 December 2023
Publisher : The Faculty of Veterinary Medicine, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2023.v15.i06.p06

Abstract

Newcastle Disease virus as the cause of tetelo disease in Indonesia or avian paramyxovirus 1 (APMV-1) is a type of oncolytic virus used as a virotherapy agent, due to its ability to lyse cancer cells in mammals including fibrosarcoma. As is known, the liver is an organ that functions in the detoxification of toxic and foreign materials that enter the body. The purpose of this study was to determine the side effects of the virulent isolate Newcastle Disease virus Gianyar-1/AK/2014 as a virotherapy agent on the histopathological features of the liver of white rats with fibrosarcoma. This study used 6 rats model of fibrosarcoma induced by carcinogens and then divided into two treatment groups consisting of three rats as replicates. Treatment 0 (P0) was injected with phosphate buffer saline (PBS) and Treatment 1 (P1) was injected with Newcastle Disease virus isolate Gianyar virulent-1/AK/2014 at a dose of 0.5 mL / 28 HA units. The histopathological features observed at 400X magnification were in the form of degenerative lesions and necrosis of hepatocyte cells and were then given a score according to their severity. The results of the Mann-Whitney U statistical test showed that there was no significant difference in the score of degenerative hepatocyte lesions between the two treatments (P>0.05). Meanwhile, necrotic lesions at P1 were lower when compared to necrotic lesions at treatment P0 (P<0.05). Based on these results it can be concluded that this virotherapy does not damage liver cells and intratumoral injection of the virus causes a better histopathological overview of the liver compared to those not given virotherapy. However, further research is still needed regarding the effect of giving the virus to liver cells in order to see the effectiveness of fibrosarcoma cancer treatment using the Newcastle Disease virus.
Efek Viroterapi Virus Newcastle Disease Isolat Gianyar-1/Ak/2014 pada Histopatologi Limpa Tikus Putih Model Fibrosarkoma Shafira Laili Aulia; Ida Bagus Oka Winaya; Anak Agung Ayu Mirah Adi
Buletin Veteriner Udayana Vol. 15 No. 6 December 2023
Publisher : The Faculty of Veterinary Medicine, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2023.v15.i06.p22

Abstract

Fibrosarcoma is cancer that originates from fibroblast cells that grow uncontrollably and indefinitely. Surgery, chemotherapy, and radiotherapy are commonly used anticancer therapies, but still have a negative effect on normal body cells, so alternative therapies are carried out using replication of oncolytic viruses, namely Newcastle disease virus. The purpose of this study was to determine the damage or negative effects on the spleen organ after virotherapy using Newcastle disease virus by observing the histopathological picture. This study used 12 rats that had been induced benzo(a)pyrene, after macroscopically observed cancer with a diameter of 3-4 mm, 6 tails with almost uniform cancer size. Grouped into two treatments, namely the control group (P0) injected with Phosphate Buffer Saline (PBS) amounting to 0.5 mL and the treatment group (P1) injected with Newcastle disease virus at a dose of 0.5 mL/28 HA units. The variables observed were congestion lesions, hemorrhage, and necrosis. The results of histopathological observations from both groups showed no specific damage to the spleen organ, but lymphocyte proliferation was observed. Based on these results, it can be concluded that virotherapy with Newcastle disease virus isolate Gianyar-1/AK/2014 has no effect on the histopathology of the spleen of white rats with fibrosarcoma.
Gambaran Histopatologi Ginjal Tikus Putih Penderita Fibrosarcoma Pasca Viroterapi Virus Newcastle Disease Ni Komang Wijayani; Anak Agung Ayu Mirah Adi; Ida Bagus Oka Winaya
Buletin Veteriner Udayana Vol. 15 No. 6 December 2023
Publisher : The Faculty of Veterinary Medicine, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2023.v15.i06.p10

Abstract

Newcastle disease virus is an oncolytic virus that has great potential as a cancer virotherapy because it can selectively infect and replicate in tumor cells. Virotherapy with ND virus can also stimulate the immune system, and lyse tumor cells with limited or non-pathogenic pathogenicity against mammals. The purpose of this study was to determine the side effects of virotherapy of the Newcastle disease virus, the virulent isolate Gianyar-1/AK/2014 on the kidneys by observing its histopathological picture. This study used a model of six fibrosarcoma rats which were randomly divided into two treatment groups where each treatment consisted of 3 rats as replicates. The non-therapeutic treatment (P0) was injected with Phosphate Buffer Saline (PBS) and the therapeutic treatment (P1) was injected with the Newcastle Disease virus isolate Gianyar-1/AK/2014 virulent at a dose of 0.5 ml 28 units of HA. Renal histopathology at P0 and P1 showed necrotic, hemorrhagic, and congestion lesions. The severity of the kidney lesions was scored and then the data obtained were analyzed using the non-parametric Mann-Whitney U test. The results of this study found that necrotic lesions were milder in P1 rats compared to P0. As for hemorrhage and congestion, there was no significant difference between treated and untreated rats. Based on the results of this study, it can be interpreted that virotherapy of the Newcastle disease virus virulent isolate Gianyar-1/AK/2014 reduced necrotic lesions (p<0.05), while for hemorrhagic and congestive lesions did not increase the severity of the lesions (p>0.05) so that This shows that virotherapy with Newcastle disease virulent isolate Gianyar-1/ AK/ 2014 does not cause any adverse effects on the kidneys.

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