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BERITA BIOLOGI

berita_biologi
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Published by Lembaga Ilmu Pengetahuan Indonesia

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Core Subject : Science, Agriculture,

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

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Articles 12 Documents

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Search results for , issue " Vol 16, No 2 (2017)" : 12 Documents clear

LUMUT SEJATI DI HUTAN ALAM PAMEUNGPEUK, TAMAN NASIONAL GUNUNG HALIMUN SALAK, JAWA BARAT [Mosses Pamengpeuk Primary Forest, Mount Halimun Salak Natiolan Park, West Java] Windadri, Florentina Indah
BERITA BIOLOGI Vol 16, No 2 (2017)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Halimun Salak National Park is one of the remaining conservation areas left in West Java. Research on diversity of Bryophytes had previously been conducted at Â Halimun Mount, Kendeng Mount and Â the lowland forest surrounding Cikaniki-Citalahab, while this present Â study was aimed to Â record the diversity of mosses at Pameungpeuk primary forest. There were 78 species Â of mosses including in 47 genera and 20 families. Syrrhopodon spiculosus Hook. & Grev. was a dominant species in the research area. Twenty seven species were new record for Halimun Salak National Park, one of which (Rhizogonium lamii Reimers) was a new record for Java and seven species were new record for Indonesia.

STUDI BIOMETRI BERDASARKAN MERISTIK DAN MORFOMETRIK IKAN GURAMI GALUR BASTAR DAN BLUESAFIR [Biometrical Study Based on Meristic and Morphometric of Giant Gouramy Strain Bastar and Bluesafir] Radona, Deni; Nafiqoh, Nunak; Arifin, Otong Zenal; Arifin, Otong Zenal
BERITA BIOLOGI Vol 16, No 2 (2017)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Identification of Gouramy is required to obtain phenotype characteristics information of the population for sustainable aquaculture Â practices. In this study phenotype diversity of giant gouramy strain bastar and bluesafir was analyzed based on Â meristic and morphometric measurement. Sample, are taken from 30 individuals of each population. The result showed that there were differences in colour patterns between the two populations, bastar population had yellowish brown body and red fins, whilst bluesafir population had brown blackish brown body and fins. The canonical function of truss morphometric analysis on 21 characteristics showed the measurement distribution of that both populations were in different quadrants. The intrapopulation similarity index showed that population of strain bluesafir had higher genetic similarity (80%) with interpopulation similarity index of 23.3%, while the intrapopulation similarity index of strain bastar was 76.7%.

PATOGENISITAS ISOLAT BAKTERI Xanthomonas oryzae pv. oryzae DAN PEMANTAUAN PENYAKIT HAWAR DAUN BAKTERI PADA PADI GALUR ISOGENIK [Pathogenicity of Xanthomonas oryzae pv. oryzae Isolates and Bacterial Leaf Blight Disease Monitoring on Rice-Near Isogenic Lines (NILs)] Suryadi, Yadi; Kadir, Triny Suryani
BERITA BIOLOGI Vol 16, No 2 (2017)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v16i2.2393

Bacterial leaf blight (BLB) caused by Xanthomonas oryzae pv. oryzae (Xoo) is an important rice disease due to its high intensity that and Â the mereased durability of its resistant variety. This study was aimed Â to determine the pathogenicity of Xoo isolates, which obtained from four regions/districts in West Java on three rice near isogenic lines (NILs) containing a single resistance (R) gene; and Â to study reaction of rice NILs/ differential genotypes containing mixture of resistant genes to Xoo population under endemic areas. Out of 22 Xoo isolates have been collected and further determined by ELISA assay. Ten Xoo isolates were selected and inoculated to identify their pathogenicity on three NILs i.e., IRBB5 (xa5), IRBB7 (Xa7) and IRBB21 (Xa21). Pathogenicity test showed that most of isolates produced large lesion, and four virulence groups were identified. Phylogenetic analysis suggested that the dominant virulent isolates were widely distributed at several district in West Java. It was shown that 11 NILs exhibited high levels of resistant reaction to the predominant Xoo pathotypes in Cianjur, while 10 lines were susceptible. The single R gene (IRBB7, IRBB21 and Java14) exhibited lower BLB severity. The IRBB50, IRBB51, IRBB52, IRBB53 lines (carrying two R genes), IRBB56, IRBB57 (carrying three R genes); IRBB64 (carrying four R genes), and IRBB66 (carrying five R genes) showed lower severity and thus produced higher resistance to Xoo. This study may implied further work to deploy effective R genes against certain Xoo pathotypes in differ Â region. Â Â

KARAKTERISASI ENZIM PROTEASE DARI BAKTERI Stenotrophomonas sp. ASAL GUNUNG BROMO, JAWA TIMUR [Characterization of Protease Enzymes of Stenotrophomonas sp. bacteria from Bromo Mountain, East Java] Soeka, Yati Sodaryati; Sulistiani, Sulistiani
BERITA BIOLOGI Vol 16, No 2 (2017)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v16i2.2940

Protease is an enzyme that can hydrolyze protein into simpler compounds, i.e peptides and amino acids. Microbial Proteases have the Â potency to be applied in industries such as detergents, skins, silver recovery, dairy, baking, beverages and pharmaceutical industries. These hydrolytic enzyme are efficiently involved in the food industry to increase the nutritional value, digestibility, palatability, flavour and reducing allergenic compounds as well as in the management of domestic and industrial wastes. The purpose of this study was to investigate the ability of Stenotrophomonas sp. isolated from Mount Bromo, East Java in producing protease. Protease activity of the bacterial isolate was qualitatively determined by formation of a clear zone surrounded their colonies on media containing skim milk (1%). We analyzed its Â proteolic activity against some effects of the incubation period, pH, temperatures and addition of monovalent and divalent metal ionsquantitatively using a spectrophotometer at ? 280 nm.The results showed that the optimum activity after incubation for two days was 315.88 U/ mL. The enzyme has continued to its activity at pH 8 (419.68 U/mL) and maintained its stability at 398.22 U/mL with activities decreased to 94.87%, while its activity at 60Â°C was 519.86 U/mL and could maintain its stability at 419.58 U/ mL, the activity decreased to 74.75%. The addition of Ca2+ could activated its enzyme activity at the amount of 424.33U/mL, while without addition of the ion its activity was 400.29 U/mL. The addition with ion MnÂ²+, K+, Na+ and Cu 2+ could act as inhibitors that might reduced the activity of the enzyme. Â

FAUNA IKAN AIR TAWAR DI PERAIRAN KAWASAN GUNUNG SAWAL, JAWA BARAT, INDONESIA [The Freshwater Fish Fauna of Sawal Mountain Region, West Java, Indonesia] Haryono, Haryono
BERITA BIOLOGI Vol 16, No 2 (2017)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

This study aimed to assess fish diversity and its related aspects in the Sawal Mountain Region, the watershed Citanduy in Ciamis, West Java, Indonesia. The study was conducted by grouping into four zones based on forest condition and the order of the river. The fisheswere sampled by using elektrofishing. This study recorded 12 species which Barbodes binotatus is the most abundant and widely local distributed, i.e. 20.09 individu/station and occupied 78.60 % of the area. Based on species status, as much as 75% species have wide geography distribution (common species) and the rest was introduced species. As much as 50% (6 species) have the potency as consumption fish, 25% (3 species) as ornamental fish, 17% (2 species) both as ornamental and consumption, and 8% (1 species) is stil unknown for the benefit.

KEANEKARAGAMAN JENIS POHON DI KAWASAN CAGAR ALAM DUNGUS IWUL, JASINGA, BOGOR [Tree Biodiversity in Dungus Iwul Nature Reserve, Jasinga, Bogor] Polosakan, Ruddy; Alhamd, Laode
BERITA BIOLOGI Vol 16, No 2 (2017)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Dungus Iwul Nature Reserve is one of the remaining conservation areas in Java and has an unique vegetation. Study on tree biodiversity was carried out in one hectare permanent plot. The results showed that there were 78 species of 35 families and 63 genera with 1219 trees per hectare. The species of Orania sylvicola was very dominating the region, with high distribution (Frequency = 100%) and the total basal area (basal area relative; BA = 50.96%). Species of O. sylvicola (Important Value; IV = 129.48%) highly covered the area, followed by Mischocarpus sundaicus (IV = 19.93%), Diospyros frutescens (IV = 9.67%), Ficus variegata (IV = 9.38%) and Knema laurina (IV = 8.51%). Family with largest number of species was Euphorbiaceae (11 species), followed by Lauraceae (7 species) and 4 species of Fabaceae, Meliaceae and Rubiaceae.

PENGARUH PENAMBAHAN GLISEROL PADA PAKAN TERHADAP PERTUMBUHAN DAN KELANGSUNGAN HIDUP IKAN NILA (Oreochromis niloticus) [Effect of Glycerol Addition into Fish Feed on the Growth and Survival Rate of Nile Tilapia (Oreochromis niloticus)] Suryaningrum, Lusi Herawati; Mulyasari, Mulyasari; Samsudin, Reza
BERITA BIOLOGI Vol 16, No 2 (2017)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

The aim of this research was to determine the optimum percentage of glycerol addition into the diets of Â Nile Tilapia (Oreochromis niloticus) on growth performance, feed convertion ratio, and survival rate. The research was conducted using 15 aquaria with the size of 60 x 50 x 45 cm3, each containing 25 O. niloticus with average initial weight of 4.63 Â± 0.15 g. The experiment was conducted using a complete random design with five treatments and three replications. Isonitrogenous (31%) and isocaloric (17 MJ.kg-1) diets were provided for 60 days of rearing period. Five diets were formulated with glycerol content of 0% (G0); 5% (G5); 10% (G10); 15% (G15), and 20% (G20). Fish were fed twice daily with experimental diet at satiation level. The result showed that addition of 10% glycerol in the diet (G10) presented the highest values for weight gain (105.85 Â± 0.40%), specific growth rate (3.44 Â± 0.01%), protein retention (27.75 Â± 0.02%), protein efficiency ratio (2.53 Â± 0.15%), lipid retention (60.44 Â± 0.03%), and feed convertion ratio (1.59 Â± 0.01%) (P < 0.05). Survival rate was not affected by dietary containing glycerol (P > 0.05). Therefore, addition of 10% glycerol into the diet gave a significant increase on growth performance and feed convertion ratio without adverse effect on survival rate of Nile Tilapia. Â

C0-CULTURE OF AMYLOLYTIC FUNGI Aspergillus niger AND OLEAGINOUS YEAST Candida orthopsilosis ON CASSAVA WASTE FOR LIPID ACCUMULATION [Akumulasi lipid oleh kultur campuran kapang Aspergillus niger dan khamir Candida orthopsilosis pada media limbah singkong] Kanti, Atit; Sudiana, I Made
BERITA BIOLOGI Vol 16, No 2 (2017)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

The objective of study was to evaluate the effectiveness of co-culture between amylolytic fungiÂ Aspergillus niger and lipid accumulating yeast Candida orthopsilosis for lipid accumulation on cassava waste. When grown in 5 % starch medium, all microbes was able to grow, but best growth was observed on Aspergillus niger, other microbes grew slower. Moderate growth was observed Â on Saccharomycopsis fibuliger.Â The two isolates were then selected for further studies. Aspergillus niger and Saccharomycopsis fibuliger were amylolytic microbes. The amylolytic activities were affected by temperature, pH and nitrogen sources.Â Optimum temperature and pH for enzyme production were 30Â°C and 7.0 respectively. Both yeast extract and sodium nitrate were good nitrogen sources for amylase production. On cassava waste, the highest biomass and total lipid content were obtained by co-culture of Aspergillus niger and lipid accumulating yeast Candida orthopsilosis. Major lipid composition was oleic acids and strearic acids. The ability of co-culture of Aspergillus niger and lipid accumulating yeast Candida orthopsilosis grew and accumulated lipid on cassava waste would suggest that these culture were candidate for biofuel production.

Pellacalyx Symphiodiscus STAFP FROM LONG BAGUN, MAHAKAM HULU: MORPHOLOGICAL CHARACTERIZATION AND ITS DISTRIBUTION [Pellacalyx Symphiodiscus Stafp dari Long Bagun, Mahakam hulu: Karakretisai Morfologi dan Persebarannya] Astuti, Inggit Puji; Susandarini, Ratna; Sari, Rismita
BERITA BIOLOGI Vol 16, No 2 (2017)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Pellacalyx is one of the genera in Rhizophoraceae, distributed from Myanmar, South China, Indonesia, Malaysia and the Philippines. In Indonesia, Pellacalyx can be found in the island of Sumatra, Kalimantan and Sulawesi. Eight species of Pellacalyx have been found Â in Indonesia, four of which them were from Kalimantan, i.e. P. axillaris, P. cristatus, P. lobbii and P. symphiodiscus. These four species generally grow in the primary and secondary forest, from lowland to an altitude of 1300 m above sea level. Information of Pellacalyx in Indonesia is still very limited , and rarely found in the wild. The last information of Pellacalyx found in West Sumatra was reported by an expedition team of the Center for Plant Conservation Bogor Botanical Garden in 2006. Â The Expert Team of the Khatulistiwa Expedition in Long Bagun, West Kutai, East Kalimantan on May 26âJune 2, 2012 found a species of Pellacalyx. Morphological characterization was then conducted based on the herbarium specimen for identification. The identification was carried out in Bogor Botanic Gardens, Center for Biological Research, Indonesian Institute of Sciences and The National Herbarium Leiden, Netherland. The results show that Pellacalyx specimen from Long Bagun is Pellacalyx symphiodiscus.Â

VARIASI GENETIK Lactobacillus fermentum Beijerink ASAL SAYUR ASIN BERDASARKAN ANALISIS RFLP 16S-23S rDNA ISR, RAPD-PCR DAN ERIC-PCR [Genetic Variation of Lactobacillus fermentum Beijerink Origin Sayur Asin Based on RFLP 16S-23S rDNA ISR, RAPD-PCR and ERIC-PCR Analysis] Sulistiani, Sulistiani; Mangunwardoyo, Wibowo; Abinawanto, Abinawanto; Sukara, Endang; Dinoto, Achmad; Salamah, Andi
BERITA BIOLOGI Vol 16, No 2 (2017)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Molecular analysis of Lactobacillus fermentum isolates is essential to understand their genetic variation in relations to their roles in sayur asin fermentation process. Combination of three molecular techniques which is restriction fragment length polymorphism (RFLP) of 16S23S rDNA intergenic spacer region (ISR), random amplified polymorphic DNA (RAPD-PCR) and an enterobacterial repetitive intergenic consensus (ERIC-PCR) analysis were performed to discriminate 19 representative isolates of L. fermentum isolated from sayur asin. The result showed that L. fermentum strain D11 is distantly related to other isolates based on RFLP using HhaI restriction enzyme and RAPDPCR analyses. In addition, both of RAPD-PCR and ERIC-PCR successfully determined the genetic variation among L. fermentum strains by exhibiting distinct 4-8 bands (800-2080 bp) and 4-10 bands (280-3050 bp), respectively. A dendogram generated from UPGMA cluster analysis of both RAPD-PCR and ERIC-PCR data showed two distinct genotypic groups exist among L. fermentum isolated from sayur asin in Indonesia.

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