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INDONESIA
BERITA BIOLOGI
Published by Lembaga Ilmu Pengetahuan Indonesia
ISSN : -     EISSN : -     DOI : -
Core Subject : Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology
Arjuna Subject : -
Articles 32 Documents
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Search results for , issue "Vol 10, No 3 (2010)" : 32 Documents clear
EFEKTIVITAS MULTIVITAMIN DAN MENIRAN (Phyllanthus neruri L.) DALAM MENURUNKAN STRES PADA DOMBA SELAMA TRANSPORTASI Satyaningtijas, Aryani S; Andriyanto, Andriyanto; Ramadhoni, Armando; Suci, Yulia; Dewi, Fitriana; Sutisna, Abadi
BERITA BIOLOGI Vol 10, No 3 (2010)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (357.538 KB) | DOI: 10.14203/beritabiologi.v10i3.755

Abstract

Sheep is one of achievable animal protein sources. Therefore, they need to be transported to/in different places to fulfill the demand of mutton. This research was conducted to know the impact of transportation on stress as presented by leucocyte differentiation (N:L ratio). Blood were taken from sheep without stress transportation as a control for 12 hours.In this research, 12 sheep were divided into 3 groups: KP (control with stress),PA (stress with combination multivitamin and meniran - Phyllanthus niruri L.), PB (stress with combination multivitamin).Blood samples were taken at 0 hour (pre-transportation), 4h, 8h, 12h (during transportation) and 24h, 48h, 72h (post-transportation) to observe leucocyte differentiation. The results showed that ratio N:L (as stress indicator) were higher at all time periods during transportation and after the transportation. Sheep as a control group had reached the peak of ratio N:L at 0 h, meanwhile sheep with combination of multivitamin and meniran at 12 h and sheep with multivitamin only at 8. It means that the sheep with combination of multivitamin and meniran treatment was more effective to response the transportation stress.
ENDOFIT Taxus sumatrana (Miquel) de Laubenfels DAN POTENSINYA DALAM MEMPRODUKSI SENYAWA BIOAKTIF SEBAGAI SUMBER ANTIOKSIDAN Sukiman, Harmastini
BERITA BIOLOGI Vol 10, No 3 (2010)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v10i3.750

Abstract

Taxus sumatrana (Miquel) de Laubenfels is known as an endanger forest tree species grown at Kerinci National Park in Jambi,Indonesia. This plant was known potential on producing taxol. Taxol is a bioactive compound, could be used as antibacterial agent and recently confirmed to cure cancer cells. For the first time in 1960, Arthur Barclay found the taxol compound from Taxus sp.(pacific yew). However to isolate the bioactive compound, huge amount of tree biomass is needed. Research on endophytes microbes which are isolated from inner tissue of Taxus sp. declared that those microbes have potential on producing antioxidant agent for drug discovery. Isolation and conservation of endophytes and selecting its potential is promising the novel of finding new drug that may be effective for treating the newly developing diseases in human. Fifteen isolates of Taxus endophytes have been successfully studied on their ability on producing antioxidant. The results showed that endophytes fungus isolates TsC-17, isolated from Taxus sumatrana grown in Cibodas Botanical Gardens-LIPI, could produce extracellular bioactive compound which performed activity of suppressing free radical, is significantly better compared to intracellular bioactive compound eventhough it is not as high as in vitamin C. The activity of suppressing free radical resulted from bioactive compound of endophyte fungus isolates TsC-17 was 20% for intracellular bioactive compound, while it was 60% for extracellular bioactive compound and 90% for vitamin C.
KOMPOSISI JENIS DAN JUMLAH BURUNG LIAR YANG DIPERDAGANGKAN DIJAWA BARAT Haryoko, Tri
BERITA BIOLOGI Vol 10, No 3 (2010)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (503.219 KB) | DOI: 10.14203/beritabiologi.v10i3.754

Abstract

Wildbird trade is a threat to the conservation of birds population in Indonesia. The main causes of wildbirds extinction are habitat loss or habitat destruction and poaching for trade.The aim of this study was to identify trade of wildbird species in West Java. The survey was carried out in October to November 2008.A total of 2080 birds (consisted of 67 species and 29 family) were traded in Bandung, Garut and Tasikmalaya areas.Bird markets in those three areas traded 1051, 496 and 533 birds respectively. The result of one way analysis of variance (anova) using SPSS 13.0 F , ll15)= 2,398 < Ftabel = 3,042, showed no significant different in the above mentioned three bird markets.
PERTUMBUHAN AKAR DUAPULUH GENOTIP PADI GOGO PADA KAHATFOSFOR DAN CEKAMAN ALUMINIUM Suhartini, Tintin
BERITA BIOLOGI Vol 10, No 3 (2010)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (752.414 KB) | DOI: 10.14203/beritabiologi.v10i3.753

Abstract

Phosphorous insufficiency is a limiting factor for rice production. P deficiency in acid soil of tropical regions causing fixation of Al. Because of it, the giving P fertilizers in acid soil become not efficient. To exploit tolerance varieties to P deficiency and Al toxicity are one of the solutions to decrease P fertilizers. The objective of this experiment was to screen twenty upland rice genotypes for P deficiency and Al toxicity, and double stress Al toxicity and P deficiency in culture solution. The experiment was arranged in a split plot design with three replications. The main plots were aluminium treatment (without and 45 ppm Al), the sub plots were P treatments (0 ppm P, 0,5 ppm P, 5 and 10 ppm P) and twenty genotypes of upland rice were sub-sub plot. The root length and root dry weight characters were used to identify genotypes tolerant to P deficiency and Al toxicity. The results indicated that based on the root length character, Sentani and K36-5-1-1-1 were tolerant to Al toxicity and double stress P deficiency and Al toxicity; while based on the root dry weight character, K36-5-1 -1-1 and NIL-C443 were tolerant to P deficiency, Al toxicity and double stress P deficiency.
KANDUNGAN KUERSETIN DAN POLA PROTEOMIK VARIETAS JAMBU B ATU (Psidium guajava L.) TUMBUH LIAR DIKAWASAN CIBINONG, BOGOR Jusuf, Eddy
BERITA BIOLOGI Vol 10, No 3 (2010)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1267.472 KB) | DOI: 10.14203/beritabiologi.v10i3.756

Abstract

This work was purposed to discover the potencies of some guava {Psidium guajava) varieties or cultivars as medicinal and fruit plant, from which the economic value then would be raised. We have collected from about 500 hectares area of our institute including the village surroundings, 35 numbers of morphologically different guavas wild growing consisting of 18 cultivars of red fruit and 17 of white fruit. Methanol extraction of dried leaves giving flavonoids, which by thin layer chromatography Kieselgel 60F2S4, the spots of quercetin of HRf > 50 obtained from all guava varieties. After extraction of each spot and measuring spectrophotometrically, the quercetin content of each plant number was found to be varied in both, among red fruit and white fruit varieties. The highest, >6.0% quercetin content obtained in two numbers of red fruit and one from white fruit and the lowest, < 0.6% obtained in two numbers of white fruit. Proteomic profile from cutted and blended fresh leaves after extraction using anti proteolytic buffer in coldness, bring us to make a phenogram giving the variability of genetic kinship, that 35 varieties were divided in three groups of kinship; first, all cultivars of red fruit, secondly sixteen cultivars of white fruit, and third one cultivar of white fruit.
OPTIMASI DAN KARAKTERISASI a-AMILASE DARI ISOLAT AKTINOMISETES YANG BERASAL DARI KALIMANTAN TIMUR Soeka, Yati Sudaryati
BERITA BIOLOGI Vol 10, No 3 (2010)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (368.489 KB) | DOI: 10.14203/beritabiologi.v10i3.751

Abstract

Forty-one actinomycetes isolates from East Kalimantan held in Microbiology Division Collection-UPI, and their ability to produce a-amylase has been assessed. Those 41 number of actinomycetes isolates performed amylolytic activity as shown by clear zone areal after being poured with iodium solution. The bacteria produced high a-amylase when was grown in media containing starch soluble 2% the a-amylase activity in media containing 8.24 U/ml. The isolate (number 7) was the most active compared to another (number 100) and it was identified as Nocardia; the activity of this enzyme obtained was 12.93 U/ml (one unit activity is defined as mol of glucose produced per ml per minute). The maximum temperature for enzyme reaction was 40°C, optimum pH was pH 7.5 the a-amylase activity were 15.76 U/ml and 31.11 U/ml, respectively. From kinetic characterization study, it was found that enzyme showed Km and Vmax value of 7.62 % (b/v) and 71.10- umol/ml/minute respectively at condition of temperature 40°C, pH 7.5 and incubation time 10 minute.
KANDUNGAN KUERSETIN DAN POLA PROTEOMIK VARIETAS JAMBU B ATU (Psidium guajava L.) TUMBUH LIAR DIKAWASAN CIBINONG, BOGOR Eddy Jusuf
BERITA BIOLOGI Vol 10, No 3 (2010)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v10i3.756

Abstract

This work was purposed to discover the potencies of some guava {Psidium guajava) varieties or cultivars as medicinal and fruit plant, from which the economic value then would be raised. We have collected from about 500 hectares area of our institute including the village surroundings, 35 numbers of morphologically different guavas wild growing consisting of 18 cultivars of red fruit and 17 of white fruit. Methanol extraction of dried leaves giving flavonoids, which by thin layer chromatography Kieselgel 60F2S4, the spots of quercetin of HRf > 50 obtained from all guava varieties. After extraction of each spot and measuring spectrophotometrically, the quercetin content of each plant number was found to be varied in both, among red fruit and white fruit varieties. The highest, >6.0% quercetin content obtained in two numbers of red fruit and one from white fruit and the lowest, < 0.6% obtained in two numbers of white fruit. Proteomic profile from cutted and blended fresh leaves after extraction using anti proteolytic buffer in coldness, bring us to make a phenogram giving the variability of genetic kinship, that 35 varieties were divided in three groups of kinship; first, all cultivars of red fruit, secondly sixteen cultivars of white fruit, and third one cultivar of white fruit.
IDENTIFIKASI GEN SELENOMETIL TRANSFERASE (smt) PADA ISOLAT Geobacillus sp. 20K YANG RESISTEN TERHADAP SELENIUM Evi Triana; Novik Nurhidayat; Titin Yulinery; Ernawati Kasim; Ratih M Dewi
BERITA BIOLOGI Vol 10, No 3 (2010)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v10i3.747

Abstract

The trace element Selenium is toxic at high concentration.Most of organisms living in selenium rich environment are selenium resistant.One of the resistance mechanisms is methylation,in which selenium is methylated and transformed to non-toxic selenium compound.The methylation is catalyzed by seleno methyltransferase (SMT) coded by smt gene. The gene are expressed by selenium tolerant plants. However, there was no available report yet on such specific gene in the bacterial genome. This study was carried out to determine smt homologous gene on selected selenium accumulator bacteria, Geobacillus sp. 20k, The smt gene of was determined by amplifying target DNA and analyses its sequences through homology search (BLAST). The result showed that the DNA and its protein part of thermophilic enzyme involved selenium metabolisms.
PENGARUH PADAT TEBAR IKAN KOAN {Ctenopharyngodon idella) TERHADAP LAJU PERAMBAHAN DAN LUAS TUTUPAN ECENG GONDOK (Eichornia crassipes) DI DANAU LIMBOTO, GORONTALO Krismono Krismono; MF Rahardjo; E Harris; ES Kartamihardja
BERITA BIOLOGI Vol 10, No 3 (2010)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v10i3.752

Abstract

Water hyacinth {Eichhornia crassipes) in Lake Limboto was covering the area about 40 to 60% in 2008. It caused a lot of problems on water transportation, fishing activities, reducing water quantity and quality. To manage the number of water hyacinth population, biological control use omnivorous species and such as grasss carp (Ctenopharingodon idella) is a promosing alternative. In this study, effect of different stocking density of grass carp on the population of water hyacinth was observed. The result showed that 200 fish was the most effective compare to other stocking density and enabled to reducing the covering area up to 10 to 20%.
KERAGAMAN DAN KELIMPAHAN JENIS KODOK SERTA HUBUNGANNYA DENGAN VEGETASI PADA LAHAN BASAH "ECOLOGY PARK", KAMPUS LIPICIBINONG Hellen Kurniati
BERITA BIOLOGI Vol 10, No 3 (2010)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v10i3.743

Abstract

Previous ecological studies have revealed the types of non-forest frog commonly occupying habitats that have been modified by humans are still severely limited. For that purpose the research was conducted in the wetland area of Ecology Park in LIPI Campus Cibinong which is located at S 06" 29' 40.2"; E 106° 51' 06.3" with 165 meters altitude above sea level (asl) over seven months (May-November 2009) by monitoring 14 times during the study period (July-November).The transect method was used to determine the effect of habitat on the diversity and abundance of frog species in the wetland area. Transect was set for 100 meters on one side of the lake where the area has a variety of habitat types. Length of 100 meters transect was divided into 10 sections with 10 meters length for each section, (length of each section was 10 meters). Every 10 meters, the type of habitat, frog species encountered and the abundance of the species were recorded. Environmental data recorded were air humidity, air temperature, water temperature and moon phase (full moon, crescent moon or the dark moon). Ten frog species which belong to 4 families, the Bufonidae (Bufo biporcatus and B. melanostictus), Microhylidae (Kaloula baleata), Ranidae (Fejervarya cancrivora, F. limnocharis, Rana chalconota, R. erythraea, R. nicobariensis and Occidozyga lima) and Rhacophoridae (Polypedates leucomystax) were found.R. erythraea, R. nicobariensis and O. lima were the dominant species and found on every visit. Furthermore, the most dominant species was R. erythraea. The results showed that environmental factors (air temperature, water temperature, humidity and conditions of the moon) did not have an impact on the presence and number of species. Abundance of three dominant species (R.erythraea, R. nicobariensis and O. lima) was strongly linked to vegetation type. Moreover, environmental factors (water temperature, humidity and the condition of moon) affected the abundance of R. nicobariensis in sub transect 2 and 5 where dominated by Eleocharis dutcis ans Leerxia hexandra. On the other hand air temperature affected the abundance of O.lima in sub transect 7, dominated by floating lotus plant Nymphaea lotus.

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