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BERITA BIOLOGI

berita_biologi
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Published by Lembaga Ilmu Pengetahuan Indonesia

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Core Subject : Science, Agriculture,

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

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Articles 34 Documents

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Search results for , issue "Vol 10, No 4 (2011)" : 34 Documents clear

STUDI PERKEMBANGAN GONAD IKAN BETOK (Anabas testudineus Bloch) DENGAN RANGSANGAN HORMON Suriansyah, Suriansyah; Sudrajat, Agus Oman; Jr, Muhammad Zairin
BERITA BIOLOGI Vol 10, No 4 (2011)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

The purpose of this study was to evaluate the effect of water in oil in water (w/o/w) emulsion containing 17a-methyltestosterone on gonad development of climbing perch. The results showed administration of 200 ug/kg 17o-methyltestosterone in w/o/w emulsion enhanced gonad development of climbing perch. In this treatment, testosterone concentration increased but estradiol-17p concentration decreased. Both hormones ranged 10,55-15,13 ng/dl and 0.96-5.66 ng/dl, respectively. This treatment also accelerated the development of egg diameter which were eggs e" 0.20 mm became 71.00% after 60 days, and development of egg nucleus position into germinal vesicle breakdown (GVBD) in day 60 was about 17.50%.

PATOGENISITAS Salmonella enterica SEROTIPE enteritidis ISOLAT LOKAL PADA ANAK AYAM DAN MENCIT Kusumaningsih, Ani
BERITA BIOLOGI Vol 10, No 4 (2011)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v10i4.764

This study was to identify the pathogenicity of local isolates of Salmonella enterica serotipe enteritidis (S. enteritidis/SE) in experimental chickens and mice. Thirty six local isolates of S. enteritidis isolated from chickens, eggs and human was inoculated 8 intra peritoneally (ip) to each 5 chickens and mice per group. Each group received 0.2 ml of broth culture containing 2 x 10Ã CFU of S. enteritidis. Chickens and mice were observed daily at 1, 2, 3, 4, 5, 6 and 7 days after inoculation for clinical signs and mortality. The result showed that the overall mortality rate of chicken and mice were 78.4% and 63.9% respectively. The highest mortality rate was in the first day after inoculation, 51.1% for chickens and 40.0% for mice, however the lowest mortality rate was in the sixth day (0.0%) after inoculation in chickens and in the seventh day (0.6%) in mice. The bacteria of S. enteritidis can be isolated from various organs (liver, heart and spleen) and caeca tonsil of every dead chickens and mice. The study showed that local isolates of S. enteritidis isolated from chickens, eggs, and human were very pathogen for experimental chickens and mice.

PEMBESARAN IKAN BAUNG {Hemibagrus nemurus) YANG DIBERI PAKAN BERBEDA DIKOLAM TANAH Huwoyon, Gleni Hasan; Suhenda, Ningrum; Nugraha, Aditiya
BERITA BIOLOGI Vol 10, No 4 (2011)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Green catfish is a commodity that is not only potential to be cultivated in the pond and in the floating net cages, but also easy to adjust to the artificial feed. The objective of this research is to evaluate the effect of different feed types (sinking and floating)on the growth performance of green catfish(Hemibagrus nemurus.One thousand and six hundred fingerlings averaging 2.50 g of 3 individual body weight were stocked in each of earthern ponds (10 x 8 x 1.2 m ). They were fed daily for four months with the same protein content (31%) and lipid (6%) diets. The feed was given in pelleted form at 8-4% of the total body weight. The result of this study showed that no significant difference (P>0.05) between different feed types. The sinking feed gave specific growth rate (2.35%), feed conversion ratio (2.49), protein retention (18.92%) and protein efficiency ratio 1.13 while the floating type gave the values 2.49%, 2.37, 20.48% and 1.21, respectively. Lipid retention of sinking feed (34.84%) is lower than that of floating type(48.39%).The survival rates ranged between 76.56-84.54%.Feed type sinking with protein content of 31% can be used in the rearing of fish in earthern pond.

UJI RESISTENSI BAKTERI TERHADAP HgCl, YANG DIISOLASIDARI TANAH PENAMBANGAN EMAS DIPONGKOR, JAWA BARAT Imamuddin, Hartati
BERITA BIOLOGI Vol 10, No 4 (2011)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Pollution of heavy metals mercury (Hg) is a serious problem especially in gold mining areas. This study aims to isolate the bacteria in the gold mining of Pongkor (West Java) and testing the bacterial resistance to mercury (Hg). The results showed that of the 5isolates tested, qualitatively only 2 isolates (P3 and P5) that resistant to HgCI2, each at a concentration of 30 and 40 ppm.Furthermore, up to 70 ppm concentration of HgCl2, P5 bacteria can still grow well even require a longer time lag phase in period.

KULTUR SEL OTAK DAN MATA IKAN KERAPU (Chromileptes altivelis) UNTUK REPLIKASI VIRAL NERVOUS NECROSIS (VNN) Sumaryam, Sumaryam; Kusyairi, Kusyairi; Oetami, Sri; Suprapto, Hari; de Vries, Garry Cores
BERITA BIOLOGI Vol 10, No 4 (2011)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

The experiment was deal with propagat cell line from encephalon and retina of grouper Chromileptes altivelis.The cell was isolate propagation of method described following by Chen et al.(1982) from carp and crussian carp fin. The cell line from enchepalon and retina of grouper grew very fast and heavy in medium L-1S.The cell line were then used for viral replication giving,isolated virus with high pathogenicity indicated by mortal effect in short time after administration. The preservation of cell line is in 10% glycerol under -80Â°C for one year. Thawing will be done every 3 months to ensure the viability of the cell.

IDENTIFIKASI 26 ISOLAT BAKTERI ENDOFITIK DAN FILOSFER PADI DENGAN ANALISIS SEKUEN16S RDNA Hidayatun, Nurul; Susilowati, Dwi N; K Mulya, K Mulya
BERITA BIOLOGI Vol 10, No 4 (2011)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

The research was subjected to identify 26 endophytic and phyllosphere bacteria isolated from 4 rice varieties using sequencing method of 16S rDNA.The sequences were then aligned with reference sequence from Gen-Bank data library by BLAST program from NCBI to find the most related identity of the isolates analysed.The sequencing analysis revealed that some isolates have a high identity to Staphylococcus and Serratia (5 isolates each), Bacillus (4 isolates), Microbacteria (3 isolates), Pseudomonas (2isolates) and Klebsiella, Acidovorak, Bulkholderiaceae, Agrobacterium and Shewanella (1 isolate each). No specific isolate dominated in both endophytic and phyllosphere group, nor host-specific isolate found in the four rice varieties.

PARTENOKARPI: BUAH TANPABIJI - APA, MENGAPADAN BAGAIMANA Sukamto, L Agus
BERITA BIOLOGI Vol 10, No 4 (2011)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Fruit growth and development occurred in plant for producing the offspring. Pollination and fertilization will form embryo and seed, which produce and supply plant growth regulators (PGR) for fruit growing. The role of seed can be exchanged with supply of POR exogenously and produce seedless fruit. Seedless fruit could be produced from triploid plant by crossing between tetraploid x diploid plants, in vitro culture of endosperm/irradiated pollen and genetic engineering or treated diploid plant by using PGR, pollen stress, chemical agent/ antibiotic and environment/ plant control. The benefit of triploid plant is quicker growth and produce of seedless fruits directly, whereas diploid plant could produce seedless fruits after fruit induction with special treatment. The other advantages of seedless fruit on certain plants are increased yield, fruit size/ weight,carotene and total sugar contents, decreased harvest period, yield fluctuation and blossom-end rot. Seedless fruit is preferential of consumer and give a higher price than the seeded fruit. Recently, seedless fruits of tomato, grape, citrus, cucumber and watermelon have been produced in commercial industries

PENGARUH BAP DAN NAA TERHADAP INDUKSI KALUS DAN KANDUNGAN ARTEMISININ DARI Artemisia annua L. Ragapadmi Purnamaningsih; Misky Ashrina
BERITA BIOLOGI Vol 10, No 4 (2011)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v10i4.766

Malaria is a global health problem that threatens 300-500 million people and kills more than one million people annually.Artemisinin, a sesquiterpen secondary plant metabolite extracted from Artemisia annua L., is a promising and potent antimalarial drug which has a remarkable activity against chloroquine resistant to Plasmodium falciparum. To counter the present low content(0.01-0.5%) of artemisinin in A. annua L.is a limitation to commercial production of the drug and uneconomical chemical synthesis. A research was conducted to induce callus production by using Murashige-Skoog (MS) medium added with NAA (0, 0.5 and 1 mg/1) and BAP (0, 0.5 dan 1 mg/1) and also to produce artemisinin from the calli. Complete Randomized Design was used in the research. Callus cultures were induced from leaf explants of A. annua. The research reports succesful approach for production of artemisinin by callus cultures of A. Annua. Medium formulation of MS basal media added with plant growth regulators BAP 0.5 mg/1 and NAA 0.5 mg/1 give the best result for callus induction than others, with callus fresh weight 844,4 mg, artemisinin content 0.73%, dry weight 216.6 mg and total weight of artemisinin 1.58 mg.

KERAGAAN HIBRIDAINTRA SPESIFIK DARIEMPAT STRAIN IKAN NILA (Oreochromis niloticus) DIKARAMBA JARING APUNG, DANAU LIDO, BOGOR Prana Mahardhika; Rudhy Gustiano; Dinar Tri Soelistyowati; MH Fariduddin Ath-thar
BERITA BIOLOGI Vol 10, No 4 (2011)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v10i4.771

The objectives of the study was to evaluate hybrid performance of sixteen reciprocal cross resulted from three strains (Red NIFI,Nirwana, BEST) and one population (Lido Lake population) of nile tilapi. The study was conducted in experimental floating netcages in Lido Lake. Fish of 25 days old ranged 0,4 ± 0.07 g were used in the experiment. Fish were reared in floating net cages sized 2x1x1 m for each cross with the density of 50 fish/cage for eight weeks (56 days). Feed were given 10% of body weight with frequency three times a day. The results showed that the highest average larvae production was 1216 from Nirwana female crossed with four different male. Growth rate of hybrids were higher than the parents (P < 0.05) and the highest was .ed NIFI x c? BEST (6,440±0,0712% ).The highest survival rate was $ Red Lido x S BEST hybrid (77.0 ±2,78%")). For heterosis, Specific Growth Rate (SGR) was 5.8188% on hybrid between Nirwana and Red Lido. While for survival rate was 15.5888% on Nirwana x BEST hybrid. Crossing between fenotipe of light color background (red) and dark color background (black) showed that red color was dominant to the black one. Pleiotric effect of color gen was detected on the SGR and survival rate.

KARAKTERISASI GENETIK IKAN KELABAU (Osteochilus kelabau) DARI BERBAGAI LOKASI DI KALIMANTAN BARAT MENGGUNAKAN METODE RAPD (Random Amplified Polymorphism DNA) Irin Iriana Kusmini; Rudy Gustiano; Mulyasari Mulyasari
BERITA BIOLOGI Vol 10, No 4 (2011)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v10i4.762

Kelabau fish (Osteochilus sp.) is an endemic fish to Kalimantan inland waters that is potential to be developed. The aim of this research was to characterize Kelabau fish and to study the data base (genetic character) of kelabau as well as its relationship. The result showed that the highest polymorphism and heterozigosity was on Kelabau Sintang and the lowest was on Kelabau Kapuas Hulu. The closest genetic distance value were between Kelabau Pontianak-Kapuas Hulu (0.5351) and the furthest were between Kelabau Pontianak-Sintang (0.6852).

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