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INDONESIA
BERITA BIOLOGI
Published by Lembaga Ilmu Pengetahuan Indonesia
ISSN : -     EISSN : -     DOI : -
Core Subject : Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology
Arjuna Subject : -
Articles 24 Documents
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Search results for , issue "Vol 14, No 1 (2015)" : 24 Documents clear
PENGARUH INOKULASI RHIZOBIUM TERHADAP PERTUMBUHAN TANAMAN KEDELAI (GLYCINE MAX L) VARIETAS WILIS DI RUMAH KACA Purwaningsih, Sri
BERITA BIOLOGI Vol 14, No 1 (2015)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v14i1.1867

Abstract

Effect of Rhizobium inoculation on the growth of Glycine max L. Wilis variety was endicated based on green house experiment.The aim of the experiment was to asses the potency of the Rhizobium strain to inccrease the growth of Glycine max L.Strains of : 1 WG, 1 WS, 1 P, 16 GH, 1 LS, 1 BK, 1 KT, 1 BR, and 11 WY were used in this research. Controls treatment were uninoculated with Rhizobium strain -1 and without urea fertilizer (K1) and uninoculated and with urea fertilizer equal 100 kg ha (K2). The research design was Completely Randomized Design with three replications for each treatment.Experiment plants were harvested after 50 days.Parameters of investigation were the dry weight of canopy, roots, nodules root, total plants, number of nodules, and ?Symbiotic Capacity?.Results of this studies showed all of the Rhizobium inoculants able to form nodule. Strain 1 KT (isolate of soil from Kalampangan, Palangkaraya,Central Kalimantan) has given the best results on the growth of Glycine max L.
PENELITIAN EKOLOGI HUTAN DI PULAU NATUNA: STATUS HARA DAUN DAN SERASAH Mirmanto, Edi
BERITA BIOLOGI Vol 14, No 1 (2015)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v14i1.1869

Abstract

Forest ecological study with special attention to leaf and litterfall nutrient status has been conducted in several forest types at Natuna Island, Riau Archipelago. Ten plots have been setup which distributed in several forest type areas. Fresh leaf was collected from some dominant tree within the plots, whereas fallen leaves collected from litter traps.Nutrient content especially for N, P, K and Ca analyzed from all combined species of both fresh and fallen leaves.Results of chemical analyses showed that all fallen leaves mineral element concentration, except for Ca,were lower than that in fresh leaf. Presentage of retranslocation of both nitrogen and phosphorus tended to be similar to some earlier studies in several tropical forests. Nitrogen and phosphorus were suspected to be limiting factors of forest growth in this area.
POTENSI TO’TOAN (Tacca leontopetaloides (L.) O.Kuntze) SEBAGAI BAHAN PANGAN DI PULAU KANGEAN, JAWA TIMUR Siti Susiarti
BERITA BIOLOGI Vol 14, No 1 (2015)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v14i1.1870

Abstract

Tacca leontopetaloides (L.) O. Kuntze has the potential as food alternative source from its tubers and therefore selected for food diversification study.This research was conducted on Kangean island using open-ended interview methods,field observations,purposive sampling and chemical analysis.Tacca leontopetaloides still not much known and utilizated by the local people in East Java.The plant has different local names for each region, such as in Madura they known as "lorkong" and "oto'o", while in Kangean island,known as "to’toan". In Kangean island, local people utilized the tuber to make flour using simple process. The flour was used as a substitute for wheat flour mainly as an ingredient for porridge, cakes “serpot” and “eped-eped (omelette)”. The species was generally grow wild in teak garden, so they can be used as an alternative crop under the teak stands. Proximate analysis of Tacca leontopetaloides tubers was also carried out and showed that they contained: 2.67 – 2.71 % of ash; 6.73 - 7.84 % of protein; 0.43 – 1.90 % of fat; 0.41 - 0.60 % of crude fibre; 77.09 - 82.65 % of carbohydrate and available energy 352.36 - 365.83 Kcal/100g based on dry weight. Mineral content was also analysed and showed the following value: 173.50 - 173.67 mg/100g of magnesium; 4.00 - 8.69 mg/100g of iron; 69.89 - 87.72 mg/100g of calsium; 904.86 - 966.74 mg/100g of potassium; and 222.59 - 270.46 mg/100g of phosphor. Hence, Tacca leontopetaloides has sufficient nutrient contents from its carbohydrate and mineral.
TOKSISITAS AKUT ORAL DUA SENYAWA BISANTRAKUINON (+)-2,2’-EPISITOSKIRIN A DAN (+)-1,1’-BISLUNATIN [Oral Acute Toxicity of Two Bisanthraquinones (+)-2,2’-Epicytoskyrin A and (+)-1,1’-Bislunatin] Praptiwi Praptiwi; Arif Nurkanto; Dewi Wulansari; Andria Agusta
BERITA BIOLOGI Vol 14, No 1 (2015)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v14i1.1858

Abstract

Bisanthraquinones (+) - 2,2'-epicytoskyrin A and (+) -1,1'bislunatin produced by the endophytic fungus Diaporthe sp. GNBP-10 showed potent antibacterial activity on in-vitro test and have the opportunity to become new antibiotics candidates. The aspects of safety and toxicity of drug candidates have to be examined before applying to human. This study was conducted to determine the safety aspects of the compounds through acute oral toxicity testing in mice (Mus musculus). Acute toxicity of (+) - 2,2'-epicytoskyrin A and (+) - 1,1'-bislunatin evaluated by the method of Up and Down Procedure with limit test at a dose of 2000 mg / kg. Results of acute toxicity test showed that the LD50 of (+) - 2,2'-epicytoskyrin A and (+) - 1,1'-bislunatin were of 1638.87 mg / kg and > 2000 mg / kg respectively. Administration of (+)- 2,2'-epicytoskyrin A resulted in increased miliari multifocal hepatitis, fatty degeneration and necrosis of liver cells, and the renal tubule epithelial degeneration. Administration of (+) - 1,1'-bislunatin at a dose of 2000 mg / kg resulted in multifocal accumulation of inflammatory cells in the liver and degeneration of cells in the islets of Langerhans although not resulting in death. The administration of those compounds indicated the changes in the organs, but based on the UN/ECE classification of LD50 value showed that (+) 2,2'- epicytoskyrin A and (+) -1,1'-bislunatin included as low acute toxicity substance.
RAGAM GENETIK TIGA POPULASI SEPAT SIAM (Trichopodus pectoralis Regan; Osphronemidae) ASAL KALIMANTAN MENGGUNAKAN ANALISIS RAPD DAN PENGUKURAN MORPHOMETRIC TRUSS Iskandariah Iskandariah; Dinar Tri Soelistyowati; Rudhy Gustiano; Irin Iriana Kusmini; Gleni Hasan Huwoyon
BERITA BIOLOGI Vol 14, No 1 (2015)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v14i1.1866

Abstract

The aim of this study was to evaluate the genetic diversity of three populations of Snakeskin Gourami (Trichopodus pectoralis;Osphronemidae)from three provinces of Indonesian Borneo:West, Central, and South Kalimantan using Random Amplified Polymorphism DNA (RAPD) and morphometric truss methods.DNA amplification using primer OPC-02,OPC-05,and OPA-09 resulted in 21 to 28 fragments with sizes ranged from 200 to 1600 bp, polymorphism value was of 7.14 to 25.00%, heterozygosity was of 0.02 to 0.11 and the genetic distance between populations was of 0.27 to 0.28.Truss morphometrics analysis showed that the coefficient of variability was ranging between 2.75 to 12.52%.There were 9 characters that can be used as diagnostic characters for Snakeskin Gourami. The intra population similarity index in Snakeskin Gourami populations from West Kalimantan was the highest (80%) followed by populations from Central Kalimantan (16.7%) and South Kalimantan (3.3%).The results of RAPD and truss morphometric analysis suggested that populations from West Kalimantan have higher genetic diversity than populations from Central and South Kalimantan.
IDENTIFIKASI GEN TERMOASIDOFILIK ALKOHOL 1 DEHIDROGENASE PADA Bacillus sp-Pjv Yulia Atika MS; I Made Artika; Novik Nurhidayat
BERITA BIOLOGI Vol 14, No 1 (2015)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v14i1.1872

Abstract

One of the constrains in the conversion process of biomass to bioethanol is the unoptimum technology for the conversion process.One of the causes is the difference between optimum temperature for enzymatic hydrolytic saccharification and for fermentation.Enzymatic hydrolysis requires high temperature and acidic pH, while fermentation operates at mild condition.Hence, thermophilic fermentative microbes are needed so that simultaneous saccharification and fermentation processes can be carried out.Alcoholdehydrogenase(ADH), an enzyme functions in the final step of fermentation, catalyzes reduction of acetaldehydeto ethanol so that it being one of indicator fermentative microbial.Protium javanicum, a typical fruit from Lombok grow at high temperature and has sour taste so that it predicted has a thermo acidophilic ADH.This study was aimed toidentify gene encoding ADHin Protiumjavanicum(Pjv) microbial isolates.ADH gene identification was carried out by DNA amplification using spesific adh primers inqPCR machine. Results showed that all isolates tested had adh gene and that of Bacillus sp-Pjv was the most efficiently amplified. Results of fermentationtest also showed that the Bacillus sp-Pjv isolate was a better ethanol-producer than the others.
KARAKTERISASI BAKTERI PENGHASIL ASAM INDOL ASETAT DAN PENGARUHNYA TERHADAP VIGOR BENIH PADI Puji Lestari; Yadi Suryadi; Dwi Ningsih Susilowati; Tri Puji Priyatno; I Made Samudra
BERITA BIOLOGI Vol 14, No 1 (2015)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v14i1.1859

Abstract

The ability to produce indole acetic acid (IAA) by endophytic bacteria is one of the basic criteria for the use of bacteria as plant growth promoter agent which is essential for the agricultural production.The objectives of this study were to evaluate the ability of 17 bacterial isolates to produce IAA and its effect on improvement of rice seed germination and molecular identification of the selected isolates based on the 16S rRNA gene. The IAA content was determined using Salkowski method measured by spectrophotometer UV-Vis and the effect of endophytic bacteria inoculation on seed germination was done by in vitro assay. Sequences of the selected isolates 16S rRNA amplified by PCR were analyzed the homology against bacterial 16S rRNA database in Genebank. IAA values ranged from 6.632 to 50.053 mg/L with the highest IAA production shown by isolate 6KJ which was followed by 4PB (41.807 mg/L). Bacterial IAA increased rice seed vigor significantly compared to control. However, bacterial inoculation with different concentrations of IAA did not significantly affect the growth of rice plants. Based on the IAA and its effect on seed vigor, 6KJ, 4PB and 2KB were selected for molecular identification. Results showed that the three isolates belonged to Bacillus genus, 6KJ as B. aryabhattai, 4PB belonging to B. cibi and 2KB having 97% homology with B. marisflavi. Further evaluation of the selected endophytic isolates producing IAA is necessary to be carried out to explore their potency as a source of hormone to promote plant growth.
PENGARUH INOKULASI RHIZOBIUM TERHADAP PERTUMBUHAN TANAMAN KEDELAI (Glycine max L) VARIETAS WILIS DI RUMAH KACA Sri Purwaningsih
BERITA BIOLOGI Vol 14, No 1 (2015)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v14i1.1867

Abstract

Effect of Rhizobium inoculation on the growth of Glycine max L. Wilis variety was endicated based on green house experiment.The aim of the experiment was to asses the potency of the Rhizobium strain to inccrease the growth of Glycine max L.Strains of : 1 WG, 1 WS, 1 P, 16 GH, 1 LS, 1 BK, 1 KT, 1 BR, and 11 WY were used in this research. Controls treatment were uninoculated with Rhizobium strain -1 and without urea fertilizer (K1) and uninoculated and with urea fertilizer equal 100 kg ha (K2). The research design was Completely Randomized Design with three replications for each treatment.Experiment plants were harvested after 50 days.Parameters of investigation were the dry weight of canopy, roots, nodules root, total plants, number of nodules, and “Symbiotic Capacity”.Results of this studies showed all of the Rhizobium inoculants able to form nodule. Strain 1 KT (isolate of soil from Kalampangan, Palangkaraya,Central Kalimantan) has given the best results on the growth of Glycine max L.
TIGA SPESIES PERONOSCLEROSPORA PENYEBAB PENYAKIT BULAI JAGUNG DI INDONESIA Ummu S. Rustiani; Meity S. Sinaga; Sri Hendrastuti Hidayat; Suryo Wiyono
BERITA BIOLOGI Vol 14, No 1 (2015)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v14i1.1860

Abstract

Downy mildew is very detrimental disease of maize production in Indonesia. Adequate information regarding the identification key based on morphological and morphometric characteristic of the causal fungi of maize downy mildew in Indonesia is limited. Study for detection and identification of morphological, morphometric, and moleculer base is urgently required. Artificial sporulation induction method performed to obtain the morphology of the fungus as a whole.The fungi were morphologically identified as symptomatic maize downy mildew collected from 13 provinces in Indonesia.Three species, namely P. maydis, P.sorghi, and P. philippinensis were identified based on the shape,size, and conidial cell wall thickness, size and number of branching coniodiophores, and long sterigmata.Confirmation by PCR succesfully amplified target DNA of P. maydis, P. sorghi, and P. philippinensis. The identification key of third species of Peronosclerospora accurately to be used in identification of Peronosclerospora causes downy mildew of maize in Indonesia.This identification key is recommended as a method of identification of the causal downy mildew of maize in Indonesia.
ISOLASI DAN AKTIVITAS PLANT GROWTH PROMOTING RHIZOBACTERIA (RHIZOBIUM, AZOSPIRILLUM, AZOTOBACTER, PSEUDOMONAS) DARI TANAH PERKEBUNAN KARET, LAMPUNG Sri Widawati
BERITA BIOLOGI Vol 14, No 1 (2015)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v14i1.1868

Abstract

Analysis of phosphate dissolving activity, P dissolved; PMEase and IAA production by Plant Growth Promoting Rhizobacteria group(PGPR)is a parameter to determine the effectiveness of these bacteria as biological organic fertilizer (BOF).This study was aimed to obtain PGPR (Rhizobium, Azospirillum, Azotobacter, Pseudomonas) that were potential as a BOF to reclamation on degraded plantation area. This study used a random sampling method for 11 sampling points (1999P1, 1999P2, 1999P3, 1999P4, 1999P5, 2007P, 2007P1,2007P2, 2007P3, 2007P4, 2007P5) in the area of rubber plant roots.Isolation and counting of bacterial populations used plate count method on selective media (YEMA, Okon, Caceres, NFB AMA, PAB) whereas phosphate dissolving activity analysis, Dissolution Efficiency Index (DE), PMEase and IAA production following the method of Nguyen, Bray, Seshadri, Tabatabai, and Gravel.The results showed that of the 11 sampling points, 11 isolates were obtained effectively consisting of four groups of bacteria, namely: Rhizobium,5 5 5 5 Azospirillum, Azotobacter and Pseudomonas with the number of population: 3.3x10 , 80x10 , 20x10 , and 14x10 cfu.The results of the analysis of DE, soluble P, PMEase acid-base, and the highest IAA obtained from the bacterium isolated from the sampling point 1999P3 and 1999P1. This potential isolates will be used to the next research on reclamation of degraded land plantation

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