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INDONESIA
BERITA BIOLOGI
Published by Lembaga Ilmu Pengetahuan Indonesia
ISSN : -     EISSN : -     DOI : -
Core Subject : Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology
Arjuna Subject : -
Articles 24 Documents
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Search results for , issue "Vol 15, No 2 (2016)" : 24 Documents clear
IDENTIFIKASI GEN / QTL (Quantitative Trait Loci) SIFAT TOLERAN CEKAMAN ALUMINIUM PADA GALUR-GALUR PADI GOGO [Identification of Gene/QTL (Quantitative Trait Loci) for Aluminium Stress Tolerant in Upland Rice Lines] Utami, Dwinita Wikan; Rosdianti, I; Yuriyah, S; Ambarwati, AD; Hanarida, I; Suwarno, Suwarno; Miftahudin, Miftahudin
BERITA BIOLOGI Vol 15, No 2 (2016)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v15i2.2276

Abstract

Program on the ability of agriculture to provide rice as a staple food tends to decrease with the reduced land area used for rice cultivation. improvement rice production should be directed to use  marginal land  as dry and acid land, where poisoning aluminium was seen as a constrain factor. Development of Aluminium (Al) tolerant variety is needed to increase rice production. This technology was environmentally friendly, cheaper and also easier for farmers adopted. The rice lines with broad genetics background which tolerant to Al toxicity has been produced by breeding program. The diversity of Indonesian local rice germplasm is a basic foundation on development of Al toxicity tolerant rice varieties. The purpose of this research was to analyze the genotype variety using 384 SNPs markers on selected Al tolerant rice lines based on bioinformatics approach. Based on the 384 SNPs markers, the total 26 selected  genotypes were clustered into 5 groups. Each group was having varied Relative Root Length (RRL), which was an Al tolerant parameter of phenotype. These groups were indicated for having different genes or QTL that were related to the Al tolerant character. The association analysis obtained 9 significant SNPs markers that can be applied as the molecular markers for assisting selection in breeding  program.
OXYGEN CONSUMPTION OF ROCK BREAM Oplegnathus fasciatus IN DIFFERENT SALINITY LEVELS AND TEMPERATURE DEGREES [Konsumsi oksigen Ikan Rock Bream Oplegnathus fasciatus pada tingkat salinitas dan suhu yang berbeda] Prakoso, Vitas Atmadi; Ryu, Jun Hyung; Min, Byung Hwa; Gustiano, Rudhy; Chang, Young Jin
BERITA BIOLOGI Vol 15, No 2 (2016)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (2930.984 KB) | DOI: 10.14203/beritabiologi.v15i2.2303

Abstract

Rock bream Oplegnathus fasciatus is one of marine fish species with high commercial value in the region of East Asia. However, studies on the metabolism related to environmental factors for this species is still lacking. This study was therefore aimed to assess the effects of salinity and temperature on oxygen consumption (OC) of rock bream (TL: 26.9±0.6 cm, BW: 477.3±61.9 g) was observed by using respiratory chamber to understand the optimal salinity and temperature for culture of rock bream. Research was conducted in Pukyong National University, Busan, South Korea. Four experimental groups were conducted to measure oxygen consumption (OC) according to salinity (35, 25, 15, and 35?5 psu) and temperature changes (15?20?25oC). The results showed that low salinity exposures tend to decrease OC of rock bream (87.1, 78.3, 66.3, and 58.5 mg O2/kg/h at 35, 25, 15, and 5 psu, respectively). Meanwhile, the oxygen consumption of rock bream increased with increasing water temperatures (35 psu: 64.7, 104.0, and 175.9 mg O2/kg/h at 15, 20, and 25oC, respectively; 25 psu: 45.8, 101.7, and 185.9 mg O2/kg/h at 15, 20, and 25oC, respectively; 15 psu: 29.8, 103.3, and 155.5 mg O2/kg/h at 15, 20, and 25oC, respectively).
OXYGEN CONSUMPTION OF ROCK BREAM Oplegnathus fasciatus IN DIFFERENT SALINITY LEVELS AND TEMPERATURE DEGREES [Konsumsi oksigen Ikan Rock Bream Oplegnathus fasciatus pada tingkat salinitas dan suhu yang berbeda] Vitas Atmadi Prakoso; Jun Hyung Ryu; Byung Hwa Min; Rudhy Gustiano; Young Jin Chang
BERITA BIOLOGI Vol 15, No 2 (2016)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v15i2.2303

Abstract

Rock bream Oplegnathus fasciatus is one of marine fish species with high commercial value in the region of East Asia. However, studies on the metabolism related to environmental factors for this species is still lacking. This study was therefore aimed to assess the effects of salinity and temperature on oxygen consumption (OC) of rock bream (TL: 26.9±0.6 cm, BW: 477.3±61.9 g) was observed by using respiratory chamber to understand the optimal salinity and temperature for culture of rock bream. Research was conducted in Pukyong National University, Busan, South Korea. Four experimental groups were conducted to measure oxygen consumption (OC) according to salinity (35, 25, 15, and 35?5 psu) and temperature changes (15?20?25oC). The results showed that low salinity exposures tend to decrease OC of rock bream (87.1, 78.3, 66.3, and 58.5 mg O2/kg/h at 35, 25, 15, and 5 psu, respectively). Meanwhile, the oxygen consumption of rock bream increased with increasing water temperatures (35 psu: 64.7, 104.0, and 175.9 mg O2/kg/h at 15, 20, and 25oC, respectively; 25 psu: 45.8, 101.7, and 185.9 mg O2/kg/h at 15, 20, and 25oC, respectively; 15 psu: 29.8, 103.3, and 155.5 mg O2/kg/h at 15, 20, and 25oC, respectively).
PENGARUH LAMA PENYIMPANAN TERHADAP PERKECAMBAHAN BIJI SAMBILOTO (Andrographis paniculata (Burm.f.) Wallich ex Nees) Solikin Solikin
BERITA BIOLOGI Vol 15, No 2 (2016)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v15i2.2281

Abstract

Sambiloto (Andrographis paniculata (Burm.f.) Wallich ex Nees) belongs to family Acanthaceae is a potential medicinal plant that long traditional (jamu).This research aimed to determine the effect of storage duration on seed germination of sambiloto. The study was conducted in a glass house of Purwodadi Botanic Garden from May 2013 - May 2015. The experiment used completely randomized design with treatments of seed storage duration, namely: S0 = no stored ; S1 = stored for 5 months ; S2 = stored for 9 months ; S3 = stored for 12 months S4 = stored for 18 months and S5 = stored 24 months. The treatments was replicated five times with 100 seeds for each replication. The seeds were sowed on river sand medium sifted with 2 mm sieve mess at about 0.5 cm deep, in polybags (15x10 cm). The polybags were placed in a plastic box 38 x 28 x 13 cm and covered by transparent plastic and black paranet. Variables observed were time, percentage and rate of the seeds germination. The results showed that the seed storage duration had significant effect on the seed germination percentage and rate of sambiloto. The highest seed germination percentage and rate was reached on treatment of 18 months storage duration (S4) with value 91.40 % and 10.72 days respectively. The slowest early seed germination was reached on the treatment S0 (no stored) namely 28,40 days after sowing. 
COOKING CHARACTERIZATION OF ARROWROOT (Maranta arundinaceae) NOODLE IN VARIOUS ARENGA STARCH SUBSTITUTION [Karakteristik Pemasakan Mie Garut (Maranta arundinaceae) Pada Variasi Subtitusi Pati Aren] Miftakhussolikhah Miftakhussolikhah; Dini Ariani; Ervika RNH; Mukhamad Angwar; Wardah Wardah; L Lola Karlina; Yudi Pranoto
BERITA BIOLOGI Vol 15, No 2 (2016)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v15i2.2602

Abstract

Arrowroot (Maranta arundinaceae) tuber has traditionally been used by Indonesian people as a source of carbohydrate in their food diet. It will be likely more preservable and flexible when the tuber is processed into flour as an alternative raw food material. Arrowroot flour should be mixed with other material that have high amylose content for making noodles, thus the noodles have good quality almost same as commercial product. The objective of this study was to investigate the effect of arenga starch substitution on the arrowroot flour noodle qualities including appearance, cooking, textural and sensory properties. Noodle was made with five variation of arrowroot flour and arenga starch composition i.e. 0:100, 25:75, 50:50, 75:25, 100:0. Characterization of noodle qualities and hedonic test of noodle were analyzed. The results show that arrowroot flour substitution affects noodle qualities such as color, brittleness, cooking time, cooking loss, swelling index, expantion ratio, tensile strength, elongation and stickiness. Noodles that have characteristic nearest to commercial products and the most favored product by panelists was the mixture of arrowroot flour 25: arenga starch 75.
ANALISIS DELIMITASI JENIS PADA Monascus Spp. MENGGUNAKAN SIDIK JARI DNA ARBITRARY PRIMER PCR Nandang Suharna; Heddy Julistiono
BERITA BIOLOGI Vol 15, No 2 (2016)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v15i2.2928

Abstract

A species delimitation analysis within Monascus spp. using Arbitrary Primer Polymerase Chain Reaction (AP PCR) DNA fingerprint was carried out. This is one of the methods used for identification and discrimination of bacterial strains within the same species. Its advantages including using single primer, independent of DNA quality, and observing amplicon shared by only some strain. This study analyzed Monascus sp. MM isolate which was originated from a source contaning high level of ethanol and two M. purpureus isolates which isolated from angkak. However, based on ITS region, 99% homology showed the unclear species delimitation. Therefore, this analysis was aimed at clarifying on the identities of Monascus species tested. The result showed DNA polymorphism among three isolates of Monascus that showed species delimitation. This study showed that species delimitation within Monascus isolates used in this analysis could be supported by AP PCR DNA fingerprinting. Therefor we suggested to use this technique or method for phylogenetic study to clarify taxonomic position of Monascus strains. 
KARAKTERISASI BAKTERI PENGHASIL a-AMILASE DAN IDENTIFIKASI ISOLAT C2 YANG DIISOLASI DARI TERASI CURAH SAMARINDA, KALMANTAN TIMUR Yati Sodaryati Soeka
BERITA BIOLOGI Vol 15, No 2 (2016)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v15i2.2290

Abstract

Enzymes a-amylase is one of the enzymes used in process of starch degradation. This present study was aimed to characterize and identify of a-amylase producing strain isolated from bulk shrimp-paste in Samarinda, East Kalimantan was carried out. An experiment was conducted to examine influences of incubation, carbon source, substrate concentration temperature for incubation, pH of media, and addition of metal ions. Identification of strain C2 was carried out by using Wizard Genomic DNA Purification Kit (Promega). The result showed that optimum activity of a-amylase from C2 after six days incubation was 18.93 U/mL. Tests on the type of substrate , soluble starch was the best source to produce a- amylase (14.51 U/mL). However, at concentration of 2 % and incubation temperature at 40°C, enzymatic activity was decreased to 12.56 U/mL and 12.79 U/mL, respectively within residual activity of 74.75%. The enzyme activity was 16.43 U/mL and its residual activity was 39.14 % when it was assayed at pH 8.5. Addition of metal ions in the form of divalent and monovalent cations (1 mM) showed that the enzyme could be activated by ion Ca2+ while ion Cu2+, Co2+, Mg2+, Zn2+ , Na+  decrease the activity of the enzyme. Identification of strain C2 using molecular characterization demonstrated that partial sequences of 16S rDNA reffered to as Bacillus subtilis.
RESPON GALUR/VARIETAS KAPAS (Gossypium hirsutum L.) TERHADAP PUPUK DOSIS N dan ZAT PENGATUR TUMBUH PADA SISTEM TUMPANGSARI DENGAN JAGUNG Fitriningdyah Tri Kadarwati; Prima Diarini Riajaya
BERITA BIOLOGI Vol 15, No 2 (2016)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v15i2.2266

Abstract

Cotton lines/varieties with high productivity require high availability of nutrients in the soil, especially nitrogen (N). To maximize the utilization and distribution of nutrients in the crops, plant growth regulator (PGR) is needed to optimize the cotton production. The research was aimed to estimate the suitable dose of N fertilizer and plant growth regulator suitable for the new cotton lines. The experiment was conducted in Mojomulyo village, Tambakromo District, Pati, Central Java, from May to October 2011.  The research was arranged in a Split Plot Design with three replications. The main plots were three lines/varieties of cotton: 99022/1; 99023/5 and Kanesia 13. The subplots were sixdose  of combination of N fertilizer with PGR namely (1) N 90 + mepiquat chloride; (2) N 90 + pachlobutrasol; (3) N 90  without PGR; (4) N 120 + mepiquat chloride; (5) N 120 + pachlobutrasol; and (6) N 120  without PGR. The results showed that under drought conditions, the use of PGR pachlobutrasol was better than mepiquat chloride. Pachlobutrasol has a higher impact on cotton production with high N fertilization (120 N/ha) resulting in 701.26 kg cotton/ha. When mepiquat chloride was added cotton production reached 665.37 kg/ha and 604.81 kg/ ha with no PGR. Production of cotton lines 99023/5 was 721.65 kg/ha higher that of Kanesia 13 (577,50 kg/ha).
NILAI HETEROSIS DAN PERANAN INDUK PADA KARAKTER PERTUMBUHAN HASIL PERSILANGAN INTERSPESIFIK Tor soro DAN Tor douronensis Deni Radona; Jojo Subagja; Irin Iriana Kusmini; Rudhy Gustiano
BERITA BIOLOGI Vol 15, No 2 (2016)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v15i2.2394

Abstract

Fish Tor is one of potential fish commodities to be developed because of their economic value. This on experiment was designed to evaluate the effect of male and female parent on morphological characters i.e length and weight growth and the value of heterosis from crosses of Tor soro and Tor douronensis. Crosses were performed in both directions (reciprocal) to form four populations. Observations lenght, weight gain, specific growth in length, specific growth in weight and value of heterosis were carried out for two months (January-March, 2014). The results showed an increase of  length, weight, specific growth in weight, specific growth in length occurred in seed produced from parent male of T. soro and female of T. douronensis with values of 0.54 cm, 0.09 g, 0.65 % and 3.79 %, rescpectively. Crossed of ? T. douronensis x ? T. soro yield heterosis value (partial) of length (40.90%) and weight (116.66%) was higher than crossed of ? T. soro x ? T. douronensis  that only have heterosis value of 0 and 33.33 %, and heterosis value (reciprocal) on the length of 20.45 % and the weight of 45.22 %.
SELEKSI JAMUR PATOGEN SERANGGA Beauveria spp. SERTA UJI PATOGENISITASNYA PADA SERANGGA INANG-WALANG (Leptocorisa acuta) Wartono Wartono; Cyntia Nirmalasari; Yadi Suryadi
BERITA BIOLOGI Vol 15, No 2 (2016)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v15i2.2271

Abstract

Rice-stink bug (Leptocorisa acuta Thumb) that attack rice crop often causing heavy damage of panicle of rice as well as decreasing either quantity or quality of grain after harvest. The objective of this research was to select 14 entomopatogenic fungi of Beauveria spp. isolates, collected from rice stink bug (L. acuta) and their pathogenicity assay to insect host L. acuta. We also aimed to study diversity of Beuveria spp. which was isolated from insect host from Situgede, Bogor. The research was conducted at Laboratory, and Green house of Center for Agricultural Biotechnology and Genetic Resources Research and Development, Bogor, in 2014. Result of the study showed that isolates from Situgede Bogor were sucessfully isolated  as Beauveria spp. based on Koch Postulat assay.  The identified fourteen isolates were morphologically confirmed as Beauveria spp. The pathogenicity test was indicated by symptoms and mortality of rice stink bug after inoculation with these entomopathogenic fungi. The Beuveria isolates Stgd2(14)1, Stgd6(14)1, Stgd7(14)2, Stgd8(14)2, and Stgd0113 were the most virulent isolates. Stgd2(14)1 provided the fastest time to kill L. acuta with LT50 values of 6.9 days. The 13 isolates of Beauveria were well amplified by ITS primers. However, no diversity was found among isolates, presumably due its narrow host range tested. 

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