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INDONESIA
BERITA BIOLOGI
Published by Lembaga Ilmu Pengetahuan Indonesia
ISSN : -     EISSN : -     DOI : -
Core Subject : Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology
Arjuna Subject : -
Articles 22 Documents
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Search results for , issue "Vol 16, No 1 (2017)" : 22 Documents clear
CLONING, EXPRESSION, AND PARTIAL PURIFICATION OF PLANTARICIN W LOCUS PRODUCED BY Lactobacillus plantarum S34 [Kloning, Ekspresi, dan Purifikasi Parsial Lokus Plantarisin W Diproduksi oleh Lactobacillus plantarum S34] Umami, Rifqiyah Nur; Mustopa, Apon Zaenal; Sukmarini, Linda; Danuri, Hasim; Putri, Andini Setyanti; Wibowo, Krisna Dwi Aria
BERITA BIOLOGI Vol 16, No 1 (2017)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (2760.052 KB) | DOI: 10.14203/beritabiologi.v16i1.2174

Abstract

Lactobacillus plantarum S34 dilaporkan mempunyai aktivitas antibakteri yang terkait dengan produksi bakteriosin. Bagian dari gen yang menyandikan salah satu lokus bakteriosin yang diproduksi oleh L. plantarum S34, disebut dengan plantarisin W (plnW), diamplifikasi dari plasmid dan dikloning menggunakan sistem vektor pGEM®-T Easy ke dalam Escherichia coli DH5?. Sekuens nukleotida plnW (± 405 pb) diidentifikasi sebagai protein integral membran. Lebih lanjut, plnW diekspresikan secara heterologus sebagai fusi protein dengan His(6)-tag tioredoksin menggunakan vektor ekspresi pET-32a(+) ke dalam E. coli BL21 (DE3) pLysS. Protein fusi rekombinan plnW terdapat dalam sitoplasma sel, tetapi selain fraksi terlarut terdapat juga fraksi tidak terlarut berupa badan inklusi. Purifikasi parsial dilakukan menggunakan kromatografi afinitas ligan Co2+ untuk fraksi terlarut dan metode elektroelusi gel poliakrilamid untuk fraksi tidak terlarut. Massa molekul berukuran kurang lebih 33 kDa terdeteksi berdasarkan pemisahan SDS-PAGE dan dikonfirmasi dengan Western blot sebagai protein fusi rekombinan plnW. Protein yang sudah terpurifikasi bermanfaat untuk mengetahui kaitan antara struktur dan fungsi bakteriosin.
PENINGKATAN PERTUMBUHAN PADI VAR CIHERANG SETELAH DIINOKULASI DENGAN Azospirillum MUTAN MULTIFUNGSI PENAMBAT N2, PELARUT P DAN PENGHASIL FITOHORMON INDOLE ACETIC ACID (IAA). Eny Ida Riyanti; Edy Listanto
BERITA BIOLOGI Vol 16, No 1 (2017)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v16i1.2171

Abstract

Modern agriculture rellies on the application of fertilizer to enhanched plant growth. Excessive application of chemical fertilizers in the long term will decrease soil fertility, therefore the use of biological fertilizers is expected to reduce the negative impact. The aim of this study was to determine the effect of multi-functional Azospirillum for N2-fixation, phosphate solubilizer  and producing phytohormone Indole Acetic Acid (IAA) on the growth of rice plants Ciherang in the pot experiment conducted in the BB Biogen’s greenhouse. Two treatments were tested: types of inoculation (not inoculated, were inoculated with the wildtype AjB 6.4.1.2 and inoculated with mutant isolates AJM 3.7.1.14), and 4 levels of fertilizer application (not fertilized, a quarter dose of paddy fertilizer recommendations, a half dose of fertilizer rice in the rice fields, and the appropriate dose of fertilizer in paddy fields). Azospirillum used is wildtype isolate AjB 6.4.1.2 and mutant isolate AJM 3.7.1.14 isolated and and mutated in BB Biogen. Ciherang rice seed inoculated with Azospirillum on the cell density of 106 cells / ml at the different seedlings tub. After the age of 14 days after planting, the seedlings were transferred into pots with three plants per pot. Parameters measured were: plant height, number of tillers, number of panicles per clump, fresh weight and dry panicles per clump, weight of 100 grains, and the content of N and P stover. Results show that inoculantion, affect significantly on the number of panicles per hill, grain weight per panicle and the dry weight of grains per panicle. 
MIKROBA ENDOFIT DARI TANAMAN SRIKAYA (Annona squamosa L.) SEBAGAI PENGHASIL ANTIMIKROBA Staphylococcus aureus DAN Candida albicans Ruth Melliawati; Sunifah Sunifah
BERITA BIOLOGI Vol 16, No 1 (2017)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v16i1.2273

Abstract

Various studies indicated that endophytic microbes lived in the plant tissues and produced antimicrobial compounds. Sugar-apple plant  (Annona squamosa L) contained alkaloids, cyanogenic glycosides, and flavonoids. The purpose of this reasearch were (1) to determine the endophytic microbes isolated from sugar-apple plant (2) to study inhibiting capabillity of endophytic isolate against Staphylococcus aureus and Candida albicans, (3) to analyze antimicrobial compounds produced by the potential endophytic isolate. Diffusion agar plate methode was used to assessed antimicrobial activity. Antimicrobial compounds were analyzed by Thin Layer Chormatography (TLC) and High Performance Liquid Chormatography (HPLC), compared with erythromycin, metronidazole and tetracycline. Twelve bacterial isolates and 24 fungus were isolated. Selected bacteria, BMC 1.1, showed the biggest clear zone on C. albicans culture on agar medium, meanwhile selected fungi, BTCK 1.1T, formed the biggest colony on S. aureus culture on agar medium. TLC and HPLC analysis showed that the Rf value of BMC 1.1 and BTCK 1.1T chloroform phase fractions was similiar to metronidazole. Metronidazole concentration in C1, C2, Ck1 and Ck2 fraction were 170.98 ppm, 18.27 ppm, 1.51 ppm and 4.14 ppm respectively.
CLONING, EXPRESSION, AND PARTIAL PURIFICATION OF PLANTARICIN W LOCUS PRODUCED BY Lactobacillus plantarum S34 Rifqiyah Nur Umami; Apon Zaenal Mustopa; Linda Sukmarini; Hasim Danuri; Andini Setyanti Putri; Krisna Dwi Aria Wibowo
BERITA BIOLOGI Vol 16, No 1 (2017)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v16i1.2174

Abstract

Lactobacillus plantarum S34 dilaporkan mempunyai aktivitas antibakteri yang terkait dengan produksi bakteriosin. Bagian dari gen yang menyandikan salah satu lokus bakteriosin yang diproduksi oleh L. plantarum S34, disebut dengan plantarisin W (plnW), diamplifikasi dari plasmid dan dikloning menggunakan sistem vektor pGEM®-T Easy ke dalam Escherichia coli DH5?. Sekuens nukleotida plnW (± 405 pb) diidentifikasi sebagai protein integral membran. Lebih lanjut, plnW diekspresikan secara heterologus sebagai fusi protein dengan His(6)-tag tioredoksin menggunakan vektor ekspresi pET-32a(+) ke dalam E. coli BL21 (DE3) pLysS. Protein fusi rekombinan plnW terdapat dalam sitoplasma sel, tetapi selain fraksi terlarut terdapat juga fraksi tidak terlarut berupa badan inklusi. Purifikasi parsial dilakukan menggunakan kromatografi afinitas ligan Co2+ untuk fraksi terlarut dan metode elektroelusi gel poliakrilamid untuk fraksi tidak terlarut. Massa molekul berukuran kurang lebih 33 kDa terdeteksi berdasarkan pemisahan SDS-PAGE dan dikonfirmasi dengan Western blot sebagai protein fusi rekombinan plnW. Protein yang sudah terpurifikasi bermanfaat untuk mengetahui kaitan antara struktur dan fungsi bakteriosin.
CATATAN KEKAYAAN JENIS GASTROPODA DI PESISIR PULAU LETI, KAWASAN BANDA SELATAN Muhammad Masrur Islami
BERITA BIOLOGI Vol 16, No 1 (2017)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v16i1.1926

Abstract

Note on species richness of gastropods in coastal area of Leti Island, southern Banda was conducted as a part of Southern Banda Expedition on October 7-16, 2015. Sample collection was carried out by hand picking in Serwaru, Tutukey village, Leti Island, Southwest Maluku.  A total of 125 individu from 25 species of gastropods was found. The highest number of individu is Clypeomorus moniliferus. Generally, gastropods found in this area were associated with rocky hard substrate.   
KUALITAS SEMEN BEKU DOMBA GARUT (Ovis aries) PENAMBAHAN SUKROSA DALAM PENGENCER SEMEN KUNING Herdis suharman
BERITA BIOLOGI Vol 16, No 1 (2017)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v16i1.2294

Abstract

The objective of this research was to examine the effect of sucrose in improving the quality of the plasma membrane intact and sperm motility of frozen semen of Garut ram. Semen was collected using artificial vagina weekly from six mature garut rams.   Immediately after initial evaluation, fresh semen was divided into four parts and diluted with Tr s extender without sucrose (T0), Tris extender + sucrose 0.2g/100 ml (T1), Tris extender + sucrose 0.4g/100 ml (T2) and Tris extender + sucrose 0.6g/100 ml (T3), respectively.  Results of this research showed that the percentage of sperm motility after thawing in T2 (49.00 ± 5.48%)  was significantly (P<0.05) higher than T0 (42.00 ± 2.74%) but was not significantly difference (P>0.05) than T1 (46.00 ± 4.18%)and T3 (48.00 ± 4.47%).   Evaluation of plasma membrane intact showed that T1 (62.33 ± 6.51%) was s gnificantly different (P <0.05) w h T0 (49.40 ± 2.19%) but was not significantly different (P> 0.05) than T2 (58.50 ± 4.97%) and T3 (56.40 ± 5.90%).  In conclusion, he addition of sucrose in semen extender  improved the quality of frozen semen of Garut ram.  Concentration of 0.2g / 100 ml is the op ma dose to improve the quality of the plasma membrane intact and motility of spermatozoa during the freezing process.
KEANEKARAGAMAN KEONG DI PULAU ENGGANO, BENGKULU UTARA Heryanto Heryanto
BERITA BIOLOGI Vol 16, No 1 (2017)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v16i1.2240

Abstract

Research on the diversity of snails in Enggano, Central Bengkulu was held on 16 April 2015 to 5 May 2015 by taking three sampling sites that are north-western part of Enggano (Kampung Bendung at Desa Banjarsari and Desa Meok), the centralpart of Enggano (Desa  Malakoni) and the south-easternpart of Enggano (Desa Kaana). Sampling was conducted using purposive sampling while the analysis using graphical method and statistical cluster and t-test. This study found 722 specimens of snails which consists of 24 species from 17 families. Most of the snails found are in small size. Land snails in the forest dominates the north-easternpart as much as 88.2%. compared to  freshwater snails (11.8%). Terrestrial and freshwater snails share amount 50% each in the forest of the central, while terrestrial snails still dominate in the forest of south-eastern part (land snails: snail freshwater = 71.4%: 28.6% ). The existence of land snails and freshwater can not be separated from the state forests themselves besides the condition of the soil and surface water as a result of the topography of the island
INDUKSI BIAK KALUS DAN BIAK SUSPENSI SEL Aquilaria malaccensis Lam. Aryani Leksonowati; Witjaksono Witjaksono; Diah Ratnadewi
BERITA BIOLOGI Vol 16, No 1 (2017)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v16i1.2687

Abstract

Aquilaria malaccensis Lam. is a plant species producing fragrant woody material that contains some resin. The compounds can be used as medicine and perfume. Sesquiterpenoid, one group of compounds has been found being synthesized and subsequently extracted from callus and cell suspension culture of Aquilaria species. The aim of this research was to find a method of producing friable calli and cell suspension cultures from leaves or internodes of A. malaccensis in vitro by using suitable plant growth regulators; cell suspension that will suitably serve as material to produce sesquiterpenoid afterwards. Calli were established in almost all treatments of auxin-cytokinin on both leaves and internod explants. The treatment of 10 mg/L IBA induced the highest percentage of callus coverage from leaves with a rather compact structure. The combined treatment of 1–2 mg/L 2.4-D and 0.2–0.3 mg/L BA induced friable callus formation in more than 80% of cultures with 27–32% callus coverage percentage.  The use of 2,4-D induced a better formation of cell suspension than Picloram, with maximum volume up to 7 mL. Cell suspension culture with fine and homogenous aggregate could be established in the medium supplemented with 0.5 –1 mg/L 2,4-D.
KARAKTERISASI PISANG REJANG TETRAPLOID HASIL INDUKSI DENGAN ORYZALIN Yuyu Suryasari Poerba; T Handayani; Witjaksono Witjaksono
BERITA BIOLOGI Vol 16, No 1 (2017)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v16i1.2210

Abstract

Triploid banana cultivar is the most desirable cultivar in banana industry because of its higher yield compared to its diploid cultivar.  The  triploid cultivar can be produced by crossing tetraploid with diploid cultivar.  However, tetraploid banana cultivar is rarely existed naturally.  Induced tetraploid of Pisang Rejang was produced using oryzalin. The present research was conducted to characterize tetraploid Pisang Rejang (Musa acuminata, AAAA genome) induced by in-vitro oryzalin treatment from diploid Pisang Rejang. Ploidy level, molecular and morphotaxonomic characters were observed.  Ploidy identification of induced Pisang Rejang was conducted using Flowcytometer.  Molecular characterization was done using RAPD and ISSR markers. Morphology characters were observed based on UPOV (2010). The results showed that tetraploid plants have similar genetic properties with their diploid controls as shown by genetic identity of 0.9901 – 0.9935. The tetraploids were differed from their diploid plants in plan habit and diameter of fruit.  The tetraploid plants produce fewer suckers, drooping leaves and broader fruits compared to its diploid control.
POTENSI SERAPAN CO2 PADA BEBERAPA JENIS KANTONG SEMAR (Nepenthes spp.) DATARAN RENDAH Muhammad Mansur
BERITA BIOLOGI Vol 16, No 1 (2017)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v16i1.2269

Abstract

Pitcher plants (Nepenthes spp.) that known as “Carnivorous plants”, has a role in CO2 absorption. However, how much contribution to the CO2 absorption has not been studied. This study was aimed to provide information on CO2 absorption of some Nepenthes species particularly lowland species. This study was conducted at Research Center for Biology-Indonesian Institute of Sciences,  Cibinong Science Center, Cibinong-West Java in June 2014 . A portable LCi ADC Bioscientific Ltd. Photosynthesis System was used to measure the absorption of CO2 directly. Measurement was conducted on 45 individuals of 15 species of Nepenthes with three replications for each species. The results showed that there was a variation of CO2 absorption rate on the lowland Nepenthes species, which was between 3.74 to 12.15 µmol m-2 s-1. The highest CO2 absorption was N. mirabilis (12.15 µmol m-2 s-1), followed by N. gracilis (9.71 µmol m-2s-1) and N. reinwardtiana (9.30 µmol m-2 s-1). While the lowest CO2 absorption occured in N. ampullaria (3.74 µmol m-2 s-1), N. hispida (3.75 µmol m -2 s-1) and N. bicalcarata (4.53 µmol m-2 s-1). Mature leaves of each species were recorded to have a highest CO2 absorption, transpiration and chlorophyll content than those of the young leaves.   

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