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INDONESIA
BERITA BIOLOGI
Published by Lembaga Ilmu Pengetahuan Indonesia
ISSN : -     EISSN : -     DOI : -
Core Subject : Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology
Arjuna Subject : -
Articles 26 Documents
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Search results for , issue "Vol 18, No 2 (2019)" : 26 Documents clear
TIPE STOMATA TIGA PULUH DUA JENIS BEGONIA ALAM INDONESIA KOLEKSI KEBUN RAYA CIBODAS Muhammad Efendi
BERITA BIOLOGI Vol 18, No 2 (2019)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v18i2.3571

Abstract

Anatomical study on native Begonia in Cibodas Botanical Garden was conducted to determine their stomata types. Stomata 32 native Begonia were prepared by semi-permanent methods. Parameters observed in this study were stomata type, number, and size of stomata. The result showed that all Begonia species have anisocytic stomata and those stomata only found in abaxial leaves. Based on number of stomata, these Begonia can be classified into two groups: single stomata and single-grouped stomata. Generally, Begonia has a single stomata, however, single-grouped stomata is a determinate character of Begonia sect. Reichenheimia and sect. Diploclinium in CBG. The differences of stomata distribution in B. ozotothrix with other members of section Petermannia are needed to be clarified further.
PERKEMBANGAN TEKNOLOGI SEL MAMALIA CHINESE HAMSTER OVARY (CHO) UNTUK PRODUKSI OBAT BERBASIS PROTEIN Adi Santoso
BERITA BIOLOGI Vol 18, No 2 (2019)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v18i2.3705

Abstract

Chinese hamsters ovary (CHO) and its derivative such as CHO-DXB11 cells, CHO-K1, CHO-DG44 and CHO-S are mammalian cells that are often used for production of therapeutic protein drugs. The CHO cells often used for protein production have several advantages including 1) host cells that are safe to use in drug production, 2) the level of production of proteins produced can be increased by amplifying genes using methotrexate (MTX), 3) having the capacity to make post-translation modificationsand 4) CHO cells can be adapted to grow in suspension. The high need for protein-based drugs triggers the development of basic knowledge and innovation in production of recombinant proteins. The impressive technological advances in CHO cell technology have made these cells can be used to produce proteins around 10 g/liter in order to meet the market demand. The first protein successfully produced using CHO mammalian cells was the therapeutic Tissue Plasminogen Activator (r-tPA, Activase) protein used for stroke patients. The presence of this drug is quickly followed by several other types of drugs. In this review, history of development of CHO cells, the contribution of CHO cells to basic research, progress of effective line cell screening and development technology are discussed.
KARAKTERISASI BIOMETRIK IKAN LELE DUMBO PERBANDINGAN DENGAN IKAN LELE AFRIKA (Clarias gariepinus BURCHELL, 1822) Bambang Iswanto; Imron Imron; Rommy Suprapto; Huria Marnis
BERITA BIOLOGI Vol 18, No 2 (2019)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v18i2.3422

Abstract

Lele Dumbo was used to be a superior clariid catfish ardely cultured in Indonesia. Despite its aquaculture success, there was uncertainty about its taxonomic identity, whether it belongs to an African catfish (Clarias gariepinus Burchell 1822) or a hybrid resulted from a hybridization between African catfish C. gariepinus and an Asian catfish C. fuscus. Though lele Dumbo was no longer popular, the genetic improvement program has successfully developed lele Sangkuriang strain, and that have recently been extensively cultivated in Indonesia. As a lele Dumbo strain, the identity of lele Sangkuriang is also uncertain, thus need to be verified. The present study aimed to investigate the similarity of lele Dumbo through morphometric and meristic characterizations using samples of lele Sangkuriang (collected from BBPBAT Sukabumi, BPBAT Cijengkol and PT STP) compared to those of African catfish C. gariepinus introduced from Thailand and Kenya. The characterizations were carried out through measurement of 20 standard morphometric characters and five meristic characters the data obtained were then analyzed using principal component analysis. The results suggested that the values of morphometric and meristic characters of all three samples of lele Sangkuriang were not different from those of African catfish C. gariepinus. Likewise, the results of principal component analysis performed on morphometric and meristic characters also revealed that morphometric and meristic characteristics of all three samples of lele Sangkuriang were not different from those of African catfish C. gariepinus. Those results revealed that biometric characteristic of both lele Dumbo and African catfish C. gariepinus was not different, thus they seem belong to the same species.     
KERAGAMAN MORFOLOGI Hoya purpureofusca Hook.f. ASAL TAMAN NASIONAL GUNUNG GEDE PANGRANGO Sri Rahayu; Kartika Ning Tyas; Hary Wawangningrum
BERITA BIOLOGI Vol 18, No 2 (2019)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v18i2.3490

Abstract

Hoya purpureofusca Hook.f. (Apocynaceae: Asclepiadoideae) has been used as an ornamental plant and the international trade of this species has become increasing. This species has restricted distribution on the high elevation of Java and Bali mountains. This epiphyte climber has succulent leaves and umbellate delicate flowers. Flower has star shape, succulent and waxy, purple., c.1 cm in diameter. The observation on the morphological characteristic is aimed to select the best sample for ornamental plant. The total of 17 observed samples were obtained from three populations at Gunung Gede Pangrango National Park, West Java, Indonesia. The selection was based on the node length, size of the leaves, and flower number, size and color. The result showed that P01 from Cibodas could be developed as leaf ornamental plant, because its shortest node and small leaves. SP1 from Selabintana could be developed as flower ornamental plant, it has numerous, larger and deep purple flower. SP1 and P01 have the farest relatives distance among all observed accessions, but still have a similarity of 75%.  
ISOLASI GEN SITRAT SINTASE BAKTERI Pseudomonas aerugenosa PS2 DARI RIZOSFER POHON KRUING (Dipterocarpus sp.) UNTUK MODEL KONSTRUKSI METABOLISME SEL MIKROALGA BERKARBOHIDRAT RENDAH Dwi Susilaningsih; Asahedi Umoro; Fredrick Onyango Ochieng; Dian Noverita Widyaningrum; Hani Susanti; Hadi Susilo; I Nengah Swastika; Utut Widyastuti
BERITA BIOLOGI Vol 18, No 2 (2019)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v18i2.2967

Abstract

Pseudomonas has the potential ability for production of citrate synthase synthesis. Pseudomonas aeruginosa could synthesize the enzyme of citrate synthase which is most likely compatible with microalgae cell. Pseudomonas aerugenosa can be found in the rhizosphere of Kruing (Dipterocarpus sp., Dipterocarpaceae). This bacteria is commonly used in agriculture purposes because it is able to synthesize organic acid such as citric acid. These organic acids are synthesized from a reaction between oxaloacetate and acetyl CoA, catalyzed by citrate synthase (CS) in the tricarboxylic acid cycle (TCA). Rhizosphere as microbial sources was obtained from Kruing (Dipterocarpus sp.), which was collected from ‘Carita’ Research Forest, Pandeglang, Banten, West Java. Citrate synthase gene-specific primers were designed based on citrate synthase gene sequences as depicted in Genbank. The isolation and amplification showed that citrate synthase can be detected and purified from Pseudomonas aeruginosa target and it consists of 1600 bp and encodes 509 amino acids. Based on BLAST (Basic Local Alignment Search Tool) analysis, CS genes that were successfully isolated had 92 % similarity with Pseudomonas aeruginosa type II citrate synthase. This CS gene is expected to be expressed in microalgae metabolism to divert the metabolism of carbohydrate formation into fatty acids. 
NISHAH KELAMIN, HUBUNGAN PANJANG-BERAT DAN UKURAN REPRODUKSI HIU Hexanchus spp. DI PERAIRAN SELATAN NUSA TENGGARA Agus Arifin Sentosa
BERITA BIOLOGI Vol 18, No 2 (2019)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v18i2.3550

Abstract

Hexanchus spp. are shark species that are often caught in the Indian Ocean southern Nusa Tenggara and its status is Near Threatened, but the information is still relatively limited. This study aimed to sex ratio, length-weight relationship and reproductive size of sixgill shark, Hexanchus spp. caught from southern Nusa Tenggara waters. This study had been carried out during 2016 at the Fishing Port of Tanjung Luar, East Lombok, West Nusa Tenggara. Shark data collection was done by measuring the length, weight, maturity and fishing data. Data were analyzed  by formula of sex ratio, length-weight relationship, length at first maturity (Lm) and length at first catch (Lc). The results showed that Hexanchus spp. caught in the southern Nusa Tenggara waters consisted of H. nakamurai (81.82%) and H. griseus (18.18%). The sex ratio of both sharks was dominated by the female. The average of the total length of H. griseus (218.3 ± 96.08 cm). It was longer than H. nakamurai (127.6 ± 30.35 cm). The length-weight relationship of H. griseus was formulated as W = 0.0195 LT 2,6344 (R² = 0,9265) and H. nakamurai with the formula W = 0.002 LT 3,1098 (R² = 0.902). Both species  have the size of Lm was bigger than the size of Lc which indicated  to their population vulnerability.  

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