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INDONESIA
BERITA BIOLOGI
Published by Lembaga Ilmu Pengetahuan Indonesia
ISSN : -     EISSN : -     DOI : -
Core Subject : Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology
Arjuna Subject : -
Articles 28 Documents
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Search results for , issue "Vol 5, No 4 (2001)" : 28 Documents clear
PERBANYAKAN DAN PENYIMPANAN KULTUR SAMBUNG NYAWA [Gynura procumbens (Lour.) Merr.] DENGAN TEKNIK IN-VITRO Hoesen, Djadja Siti Hazar
BERITA BIOLOGI Vol 5, No 4 (2001)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (350.493 KB) | DOI: 10.14203/beritabiologi.v5i4.1122

Abstract

Sambung nyawa [Gynura procumbens(Lour.)Merr.]is one of the traditional medicine sources.The plants are not cultivated intensively in the field. One of the efforts to maintain and to propagate this species is by multiplication through tissue culture method. Nodes explants were cultured in MS normal and half strength concentration macroelements, supplemented with microelements and vitamins; combination of cytokinin BA (2 mg/1), thidiazuron (0.01 mg/1 and 0.1 mg/1) and adenine sulphate (5 mg/1).Auxin (2,4D 0.5 mg/1) in combination were added in media as treatments and activated charcoal (2 g/1) as antioxidant. Young leaves explants were also cultured in the same basic medium (MS and A MS) in treatments with cytokinin (BA and thidiazuron).The results from nodes and young leaves explant indicated that the highest number of survival cultures were obtained from combination between BA (2 mg/1) and thidiazuron (0.01 mg/1) in MS normal strength basic medium.In acclimatization stage, 100% of plantlets survived and successfully transplanted to soil medium in the field; maintenance study indicated that subculture was prolonged until 52 weeks.
PENGARUH PEMBERIAN SUPLEMEN MADU PADA INDUK MENCIT TERHADAP RASIO JENIS KELAMIN ANAKNYA Riyanto, Awal
BERITA BIOLOGI Vol 5, No 4 (2001)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (109.382 KB) | DOI: 10.14203/beritabiologi.v5i4.1130

Abstract

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ANATOMI DAN KANDUNGAN KLOROFIL DAUN KELADI TIKUS {Thyponiumflageliforme (Lodd.) Bl.} PADA BERBAGAI INTENSITAS CAHAYA Juhaeti, Titi
BERITA BIOLOGI Vol 5, No 4 (2001)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (159.398 KB) | DOI: 10.14203/beritabiologi.v5i4.1131

Abstract

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STANDAR INTERNASIONAL PENGELOLAAN KOLEKSIILMIAH IKAN Tjakrawidjaja, Agus Hadiat
BERITA BIOLOGI Vol 5, No 4 (2001)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (8068.328 KB) | DOI: 10.14203/beritabiologi.v5i4.1132

Abstract

Indonesia, in Rio de Janeiros United Nations meeting (1992) was considered as one of the world mega biodiversity regions.The biodiversity richness also shown in the high variation of her fish species both fresh water and marine.Collection of scientific materials in a zoological museum is an imvaluable treasure of a country, and important in the study and conservation of biodiversity. This paper discusses aspects of international standard on fish scientific collection management including steps started from initial collection, processing, delivery, maintenance and material exchange for scientific purpose.
PERBANYAKAN TANAMAN KRISAN (Chrysanthemum morifolium) MELALUI KULTUR JARINGAN Ita Dwimahyani; S Gandanegara
BERITA BIOLOGI Vol 5, No 4 (2001)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v5i4.1127

Abstract

Vegetatively propagated Chrysanthemum morifolium has been carried out by tissue culture.Explants derived from shoot-tip of three chrysant genotypes were used for the experiment. Shoot-tips taken from terminal branch and sterilized with 0.05% HgCb for 30 minutes and rinsed with distilled water three times.Shoot-tips were cultured on MS regeneration medium containing 1 mg/1 BAP and 0.02 mg/1 NAA.Shoot regeneration occurred 20 days after cultured.A number of tiny shoots were then transferred to fresh regeneration medium for 3 weeks and regenerated shoots were calculated.Results showed that plant genotypes had different response into plant regeneration. Genotype Ku-Ch had better response compared to other two genotypes which shown from number of plantlets produced by this genotype on the third sub-culture.
STANDAR INTERNASIONAL PENGELOLAAN KOLEKSIILMIAH IKAN Agus Hadiat Tjakrawidjaja
BERITA BIOLOGI Vol 5, No 4 (2001)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v5i4.1132

Abstract

Indonesia, in Rio de Janeiro's United Nations meeting (1992) was considered as one of the world mega biodiversity regions.The biodiversity richness also shown in the high variation of her fish species both fresh water and marine.Collection of scientific materials in a zoological museum is an imvaluable treasure of a country, and important in the study and conservation of biodiversity. This paper discusses aspects of international standard on fish scientific collection management including steps started from initial collection, processing, delivery, maintenance and material exchange for scientific purpose.
PERBANYAKAN DAN PENYIMPANAN KULTUR SAMBUNG NYAWA [Gynura procumbens (Lour.) Merr.] DENGAN TEKNIK IN-VITRO Djadja Siti Hazar Hoesen
BERITA BIOLOGI Vol 5, No 4 (2001)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v5i4.1122

Abstract

Sambung nyawa [Gynura procumbens(Lour.)Merr.]is one of the traditional medicine sources.The plants are not cultivated intensively in the field. One of the efforts to maintain and to propagate this species is by multiplication through tissue culture method. Nodes explants were cultured in MS normal and half strength concentration macroelements, supplemented with microelements and vitamins; combination of cytokinin BA (2 mg/1), thidiazuron (0.01 mg/1 and 0.1 mg/1) and adenine sulphate (5 mg/1).Auxin (2,4D 0.5 mg/1) in combination were added in media as treatments and activated charcoal (2 g/1) as antioxidant. Young leaves explants were also cultured in the same basic medium (MS and 'A MS) in treatments with cytokinin (BA and thidiazuron).The results from nodes and young leaves explant indicated that the highest number of survival cultures were obtained from combination between BA (2 mg/1) and thidiazuron (0.01 mg/1) in MS normal strength basic medium.In acclimatization stage, 100% of plantlets survived and successfully transplanted to soil medium in the field; maintenance study indicated that subculture was prolonged until 52 weeks.
EKOLOGI HUTAN PAMAH BARITO ULU, KALIMANTAN TENGAH: RESPON TERHADAP PEMUPUKAN NITROGEN DAN FOSFOR Edi Mirmanto
BERITA BIOLOGI Vol 5, No 4 (2001)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v5i4.1128

Abstract

A fertilization was set up in September 1993 in species-rich dipterocarp forest in the Barito Ulu study site in Central Kalimantan.The experiment was unusual in that previously reported fertilizer experiments have been made in montane forest. The following treatments were applied: control, +N, +P and +NP. There were five blocks of four 50m x 50m plots with a separate treatment for each plot. Fine litterfall was collected on all of the plots from May 1994 for one year. There was evidence of higher litterfall quantities and increased of P and N in the litterfall. All trees (> 10 cm dbh) were measured in August 1993 and in July 1994 and there was a clear girth increment response of some dipterocarp species to +NP and small size of trees to +N.
PENGARUH PEMBERIAN ELISITOR EKSTRAK KHAMIR Saccharomyces cerevisiae Hansen TERHADAP KANDUNGAN AJMALISIN DALAM KULTUR AGREGAT SEL Catharanthus roseus (L.) G. Don. Jujun Ratnasari; Arbayah H Siregar; Rizkita RE
BERITA BIOLOGI Vol 5, No 4 (2001)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v5i4.1118

Abstract

There were many ways to obtain high production of secondary metabolites in plant tissue culture; among the other is elicitation.An experiment to study the effect of elicitor derived from Saccharomyces cerevisiae Hansen extract on ajmalicine content in cell aggregates culture of Catharanthus roseus (L.) G.Don, has been conducted. The media used for callus induction and cell aggregates culture were Zenk 6 5(1977) with addition of 2.5 x 10" M Naphthalene Acetic Acid (NAA) and 10' M 6-Benzilaminopurine (BAP).The cell aggregates culturewas subcultured three times and then elicitated with elicitor derived from autoclaved 5.cerevisiae extract at concentrations 0.5, 1.0, 2.5%,and harvested at 18, 24,and 36 hours after elicitation.The ajmalicine was analyzed qualitatively and quantitatively by using High Pressure Liquid Chromatography (HPLC) connected to chromatopack CR-7A Plus. The cell aggregates of C. roseus culture produced ajmalicine both in the cells and the media.The result of elicitation showed that ajmalicine content was influenced significantly by concentration and harvesting time. The highest ajmalicine content in the cell aggregates was 25.288 ± 0.102 jig/g dw, whilst that in media was 524.600 ± 0.566 \\ML. The optimum concentration of S. cerevisiae extract was 0.5%, and the best harvesting time was 24 hours.
TOKSISITAS GLISEROL ATAU SUKROSA PADA SEL KHAMIR accharomyces cerevisiae YANG DISEMPAN PADA SUHU RENDAH BEKU Heddy Julistiono
BERITA BIOLOGI Vol 5, No 4 (2001)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v5i4.1123

Abstract

The effect of cryoprotectants glycerol and sucrose on cell viability and fermentation rate of Saccharomyces cerevisiae after freezing (-30 °C) and Chawing (30 °C) were studied.Both freezing and thawing were done rapidly. The mortality of cells treated with low concentrations of cryoprotectans (2.5, 5, and 10 %) after 15 and 30 days of cryopreservation, was remarkably higher than that of control and higher concentration (20% and 40%).Glycerol or sucrose with concentration of 20 % and 40 % protected cells from severe mortality only after 90 days of cryopreservation.Fermentation rate of cells treated with 20 % or 40 % of the two cryoprotectants were higher than that of control after 60 and 90 days of cryopreservation.The data indicated that in certain circumstance cryoprotectant could be toxic for the cells during freezing and thawing.Since biomembrane is not permeable to sucrose, therefore we proposed that target of sucrose toxicity may be extracellular, whereas glycerol, which penetrate cells,targets of the toxicity may be both extracellular and intracellular domains.Interaction between cryoprotectant and cell membranes is discussed.

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