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BERITA BIOLOGI

berita_biologi
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Published by Lembaga Ilmu Pengetahuan Indonesia

ISSN : - EISSN : - DOI : -

Core Subject : Science, Agriculture,

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

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Articles 38 Documents

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Search results for , issue "Vol 6, No 3 (2002)" : 38 Documents clear

STATUS MIKORIZA TUMBUHAN DI GRESSWELL NATURE RESERVE, MELBOURNE, VICTORIA, AUSTRALIA Kramadibrata, K
BERITA BIOLOGI Vol 6, No 3 (2002)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

A study on the mycorrhizal status of plants growing in an urban forest reserve which has still patches of remnant native vegetation was conducted in the Gresswell Nature Reserve at Bundoora, Victoria, Australia.The results showed that there were 24 families comprising 53 species.Of the 53 species,32 had AM alone, 15 had AM and ectomycorrhiza,one had ectomycorrhiza alone and five were non-mycorrhizal. There were 26 taxa AM fungi associated with plant roots i.e.,four taxa of Acaulospora, one of Entrophospora, 19 of Glomus and two of Scutellospora.

APPLICATION OF MICROSATELLITE MARKERS IN IDENTIFICATION AND PARENTAGE ASSIGNMENT OF SHEEP Margawati, ET; Tammen, Tammen; Jones, M; Raadsma, HW
BERITA BIOLOGI Vol 6, No 3 (2002)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

The accuracy of the parentage reference is the early step of information needed for genotyping study.Inaccurate data of recording sheep numbers is often occurred. Sometimes, we found sheep or lambs with uncertain original parents. This study was designed to identify the parentages of sheep and to find the putative parents of lambs. Using an advance DNA technology in molecular genetics particularly in genotyping study, those purposes can be clarified. DNA of sheep were isolated from fresh blood samples in EDTA using a high salt method. A concentration of 50ng/(il DNA was used for amplification. Several microsatellite markers were applied in the amplification. Using a DNA analyser and sequencer, PCR products were analysed to look at the figures of alleles (bands). Results showed that using microsatellite markers could inform that parents of several experimental lambs were incorrect. It was found that putative parents of some lambs were generated from incorrect parents. This study suggests that microsatellite marker can be used to clarify unknown original parents.

KOMPONEN KIMIA MINYAK ATSIRIDAUN TIGA JENIS PIPERACEAE Jamal, Yuliasri
BERITA BIOLOGI Vol 6, No 3 (2002)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Chemical analysis of leaves essential oil on three Piper species, P. malamiri, P. baccatum and P. majusculum was conducted using gas chromatography and mass spectrometry (GCMS) method.Chromatogram result showed 52, 56 and 47 peaks on leaves essential oil of P.malamiri, P.baccatum and P.majusculum respectively. Based on the peaks,it is known that isocaryofilene is the major, common and as the highest component found in the three essential oils, beside several other major components.

PERSEBARAN GEOGRAFI JENIS-JENIS Pimelodendron (EUPHORBIACEAE) DI MALESIA Djarwaningsih, Tutie
BERITA BIOLOGI Vol 6, No 3 (2002)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v6i3.1225

The analysis of geographical distribution of Pimelodendron in Malesia based especially on data from the revision study of Pimelodendron (Euphorbiaceae) in Malesia. An analysis of distribution indicated that the highest number of species can be recognized, i.e. in Sumatera four species P.griffithianum, P. macrocarpum. P. zoanthogyne, and P. yatesianum; Peninsular Malaysia and Borneo have three species P. griffithianum, P. macrocarpum, and P. zoanthogyne; while Celebes, Moluccas, Lesser Sunda Islands, New Guinea, Solomon Islands and tropical Australia have one species (P. amboinicum). The geographical distribution indicated that the Malesian region can be considered as the centre of distribution of Pimelodendron.

PENGARUH VARIASITOPOGRAFIMIKRO TERHADAP VEGETASI HUTAN GAMBUT DI BENGKALIS, RIAU Siregar, Mustaid
BERITA BIOLOGI Vol 6, No 3 (2002)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Peat forest vegetation in Bengkalis, Riau Province was studied using plot method. Two plots of 60 m x 40 m were placed at two locations which were different in peat physical condition (Plot 1: hemofibric, > 4 m deep, inundated; and Plot 2: hemosapric, 3 m deep, rarely inundated). The results showed that there were 79 species (diameter > 2 cm) included 49 species of trees (dbh > 10 cm) and 52 species of saplings (diameter 2-9,9 cm). Total number of species in plot 1 was higher (55 species; index diversity- H=3.048) than that of plot 2 (39 species; H=2.606), but, density and total basal area in plot 1 was lower than that of plot 2. Species similarity index in both plots was 19%.The most dominant tree species in plot 1 were Baccaurea macrocarpa, Ficus sp. and Palaquium hexandrum, whereas in plot 2 were Calophyllum soulattri, Ganua motleyana and Palaquium burckii. The influence of physical and peat soil nutrient on structure and species composition will be discussed in this paper.

UJI PREFERENSI TUMBUHAN INANG BEBERAPA POPULASI KUMBANG LEMBING Epilaehna. aff. emarginata (COLEOPTERA; COCCINELLIDAE; EPILACHNINAE) Kahono, Sih; Pujiastuti, Liliek Endang; Fujiyama, Naoyuki; Nakano, Susumu
BERITA BIOLOGI Vol 6, No 3 (2002)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v6i3.1221

Epilaehna sp. aff. emarginata had been considered as a specialist on Mikania micrantha (Compositae). However, recently its oceurrence on Leucas lavandulifolia (Labiatae) was reported. Preliminary field observations of some beetle populations showed that they had different on the host plant utilizations. However, the result was not sufficient to interpret the food preference of the beetles. These experiments weremade in order to clarify those previous observations.Experiments on adult food preference was investigated under uncontrolled room conditions.Four beetle populations (Bogor, Cibinong, Klaten and Malang) were subjected to choice tests offering Mikania micrantha (Compositae) and Leucas lavandulifolia. These experiments indicated that individual beetle has similar trend on food preference within each population. Bogor and Cibinong populations preferred to feed on both M. micrantha and L. lavandulifolia while. Klaten and Malang populations preferred exclusively on L. lavandulifolia. The populations showed different food preferences on the different geographical conditions of Java.

POLA PRODUKSI AJMALISIN DARIKULTUR AGREGAT SEL Catharanthus roseus (L) G Don. DALAM BIOREAKTOR Muspiah, Aida; Esyanti, Rizkita Rahmi; Siregar, Arbayah H; Tritama, Erman
BERITA BIOLOGI Vol 6, No 3 (2002)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v6i3.1212

A research has been conducted to optimize the rate of aeration and initial cell aggregates weight in the production of ajmalicine in Catharanthus roseus cell culture in bioreactor. Catharanthus roseus culture were grown in Zenk medium with the addition of 2.5 x 10"6 M NAA (naphthalene acetic acid) and 10s M BAP (benzyl amino purine). Cell aggregates were subcultured two times before transferring 20 and 30 g/fw of cell aggregates into bioreactor, respectively, and aerated with the rate of 0.2460 L/min and 0,3405 L/min, respectively. The pattern of ajmalicine production in bioreactor were observed every 3 days for 24 days.Qualitative and quantitative analysis were conducted using HPLC connected to Cromatopac CL-7APlus. The results showed that the cell aggregates and medium contain ajmalicine. The highest concentration was obtained in combination of 30 g/fw and 0.3405 L/min aeration compare to 20 g/fw - 0,246 L/min, 20 g/fw- 0,3405 L/min, as well as 30 g/fw - 0,2460 L/min. The highest ajmalicine content in cell aggregates was obtained on the 12"d days (79.23ug/g) whilst in medium was obtained in the 18th days (981.15 ug/L).

PENGEMBANGAN TEKNOLOGI PANEN HUJAN DAN ALIRAN PERMUKAAN UNTUK MENEKAN RESIKO KEKERINGAN DAN MENINGKATKAN PRODUKTIVITAS LAHAN (Studi kasus di Sub DAS Bunder, Kabupaten Gunungkidul, Provinsi DIY) Irianto, Gatot; Pujilestari, Nurwindah
BERITA BIOLOGI Vol 6, No 3 (2002)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Limited water supply on dry season at dry terrain,cause plantation index and plant productivity low. Mean while yearly water volume are potentially enough for twice plant even more if they manage well. Because of that reason using and developing water resource by rainfall and run off harvesting technology, increasing productivity and maintain dry terrain farm operations system at watershed system are approximation strategy needed to put into practice. That strategy can put into practice through hydrologist characteristic modification at river flow area with escalating capacity and capability watershed distribution to minimalist inundation risk and dried out at dry season. Research result at sub watershed Bunder, Wonosari, DIY by way of building two level dam at river bed (hydrology network) can producing 11.6% (channel reservoir 1) and 18.4% (channel reservoir 2) surface flow from existing river-bed and this result can supply water irrigation at least 3 times in planting season rice-secondary crop- secondary crop). If cropping pattern change to rice-rice-secondary crop then run off harvest need to increase become 100% at upper subwatershed (channel reservoir 1) and 19% at lower subwatershed, with condition at MK 1.irrigation area that can be plant with rice only 0.19 ha and the rest of it can be plant with peanut. Rice productivity at rice field are 2.4 ton/ha.

KAJIAN PENGARUH SEL IMOBIL Arthrobacter NRRL B-3728 TERHADAP AKTIVITAS DAN STABILITAS ENZIM GLUKOSA ISOMERASE Triantarti, Triantarti; M, Hendro Santoso
BERITA BIOLOGI Vol 6, No 3 (2002)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

This project focused on the immobilization of glucose isomerase (GI) from Arthrobacter B-3728. The whole cells immobilization technique using glutaraldehyde and gelatine type B220 was used in this research. The optimum time for harvesting Arthrobacter cells was determined before immobilization.The Arthrobacter cells were harvested after 56-72 h fermentation when the GI activity reaching 0.515-0.603 U/ml broth. The best treatment for cell immobilization was found using 5% gelatine when the GI activity reaching 0,888 U/ g immobilized cells. The optimum pH was not changed (pH 8) but the sensitivity to the pH was changed for immobilized cells compared to the free cells. However, the stability of the immobilized cells was lower compared to the free cells for long isomerization process. Further research are still needed for the development of immobilization technique for Arthrobacter B-3728.

APPLICATION OF MICROSATELLITE MARKERS IN IDENTIFICATION AND PARENTAGE ASSIGNMENT OF SHEEP ET Margawati; Tammen Tammen; M Jones; HW Raadsma
BERITA BIOLOGI Vol 6, No 3 (2002)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v6i3.1220

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