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INDONESIA
BERITA BIOLOGI
Published by Lembaga Ilmu Pengetahuan Indonesia
ISSN : -     EISSN : -     DOI : -
Core Subject : Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology
Arjuna Subject : -
Articles 30 Documents
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Search results for , issue "Vol 6, No 4 (2003)" : 30 Documents clear
VARIASI MORFOLOGI JENIS INANG Subulura andersoni Cobblod, 1887 DI INDONESIA DAN DESKRIPSI Subulura sipiroki n.sp. Endang Purwaningsih
BERITA BIOLOGI Vol 6, No 4 (2003)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v6i4.3451

Abstract

Subulura andersoni (Nematoda: Subuluroidae) recovered from some of Muridae in several places in Indonesia was observed for morphological variation. There kinds of tail morphology were found e.g tail with short and bluntly rounded spike, tail with longer and sharply pointed spike, and tail with antero ventrally curve spike, The new species of Subulura sipiroki n.sp. recovered from Leopoldamys sabanus in Sumatera also described.
PENAPISAN FITOKIMA DAN BILANGAN PEROKSIDA (POV) TIGA JENIS TUMBUHAN FAMILI MORACEACE (Artocarpus sp., A.elasticus, dan Ficus sp.) DARI TAMAN NASIONAL BOGANI NANI WARTABONE (SULUT) Mindarti Harapini; Praptiwi Praptiwi
BERITA BIOLOGI Vol 6, No 4 (2003)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v6i4.3456

Abstract

The  aim of study were to determine the chemical compounds and peroxide values (POV) of methanol extract of three plants species belongs to Moraceae family (Artocarpus (Artocarpus sp., A.elasticus, dan Ficus sp.). Their chemical compounds were identified by Cuiley method, while peroxide value was done by iodometri titration. Methanol extract percentage of Artocarpus sp., A.elasticus, dan Ficus sp. were 20.80%, 3.10% and 4.10% respectively. All of the extract tested contained essential oil, sterol and triterpenoid, aglicon flavonoid, tannin, flavonoid glicoside and saponin. POV of Artocarpus sp., A.elasticus, dan Ficus sp. were 325.24, 306.25 and 1580.07 respectively and POV of q tocopherol was 363.96. So, it can be concluded taht all of three methanol extract act as reductor, because their POV less that POV of q tocopherol and might be act antioxidant. These correalted with their chemical compounds such as flavonoid, tannin and peroxide sugar.
IDENTIFIKASI GALUR Ralstonia solanacearum DENGAN HIBRIDASI SLOT BLOT DNA Y suryadi
BERITA BIOLOGI Vol 6, No 4 (2003)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v6i4.3447

Abstract

R. solanacearum (RS), the casual agent of bacterial wilt is one of the most destructive pathogen in the tropical and sub tropical areas that affected various economic crops. Since the pathogen is very diverse, it is necessary to identify the variation of RS isolates. The Polymerase Chain Reaction (PCR) was used in this study to amplify 16S rRNA gane. Two DNA primer pairs namely pr#a and pr#b were used as probe to identify thirtyfour strains of Rs respresenting different biovars isolated from various host. The DNA probes were labelled using digoxygenin and deteced by DNA slot blot hybridization. Resutl showed that the DNA probes colud hybridzed specifically with target DNA, hence distinguish variation of Rs isolates. This assay will be of futher used in the strain identification of Rs from wide range of host from various geographic distribution.
IDENTIFIKASI SECARA CEPAT BAHAN BIOAKTIF PADA TUMNUHAN DI LAPANGAN Chairul Chairul
BERITA BIOLOGI Vol 6, No 4 (2003)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v6i4.3461

Abstract

New drug discovery from natural products (bio-prospecting) is not an easy works and takes time, big budget, human resources etc. some important approaches must be taken in order to get success. A preliminary observation of biologycally active components is impotant approach in order that more selective in collecting research materials in the field. This approach is purposed to pacilitate (make easier) the next step research process later in the laboratory level. Several Methods of preliminary observaton of biologyvcally active components had been carried out in the field, but the general guideline of fast observation had been used e.g. 1). Etno-botany (Etnomedicine and Etnopharmacology), 2). Chemotaxonomy, 3). Organoleptic, 4). Chemical (reagent kit) and 5).Simple bioassay in the field (fish posion test, insecticide). Besides that collecting the herbarium specimen as well as research materials for any scienfitic and laboratory works. This paper described these approaches in order to make useful methods to researchers, who go to the field and they could collect more selective research materials before bring them to laboratory.
PERBANYAKAN IN VITRO KELADI TIKUS (Typhonium flagelliforme (Lodd) BI.), TANAMANA YANG BERPOTENSI SEBAGAI OBAT KANKER Maria Imelda
BERITA BIOLOGI Vol 6, No 4 (2003)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v6i4.3452

Abstract

Keladi tikus (Typhonium flagelliforme (Lodd) BI.) of the Araceae generally grows wild in the open or in slightly shaded areas. The active compounds of the plant have not yet been known, however its potential for cancer medication has been reported by several media. Since the plant is being hevaly removed in their habitat, research for adoption of in vitro technique for production of their planting materilals is needed. Lateral shoots of a 2-cm tuber, were sterilized with 30% sodium hypochlorite for 15 minutes. After several rinses with sterile distilled water, the shoots were cultured on a soild Murashige & Skoog (MS) medium supplemented with BAP (0,25;0,5;0,75;1,0 mg/l) in combination with IBA (0,25;0,5;0,75;1,0 mg/l). All cultures were incubated at 26 C under fluorestcent lights of 16 hours/day. Rooting of shoots was induced on MS medium without growth regulators and the plantets were established in a potting medium of sand,compost and soil (1:1:1). The result showed that the highest rate of shoot proliferation and growth was on MS medium supplemented with 0,5 mg/l 1 BAP and 0.25 mg/l IBA. Using this medium,  a sixteen-fold increase in shoot multiplication can be achieved with survival rate of 95%.
FLUKTUASI AIR DALAM TUMBUHAN (PLANT WATER RELATIONS) DAN STABILITAS TAMAN NASIONAL GUNUNG HALIMUN: KIANAK { CASTANOPSIS ACCUMINATATISSIMA (BL.) DC} B Paul Naiola; D Siti Hazar Hoesen
BERITA BIOLOGI Vol 6, No 4 (2003)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v6i4.3457

Abstract

Information on the water relations of tropical plant species are less available,. This paper deals with a study of the water relations of tropical forest tree species, Kianak {Castanopsis Accuminatatissima (Bl.) DS}], a habitant of Gunung Halimun Natinal park. The purpose of this study held in October 2001, is to understand the water relation fluctuations of tropical tree species, especially those grown in a stablie ecosystem such as National Park. Parametes meansured are water potential components incluiding total water potentials. Meansurement were conducted by Pressure Chamber. The results shows that Kianak kept their average total water potentila at -0.48 MPa, while their average  osmotic potential were held at -1.54 MPa, thus they retained their average cells/tissues turgor pressure at 1.06 MPa. Diurnal water potentials shows no significant flutuations (-0.47 MPa at 10 am,-0.48 MPa at 1 pm and -0.48 MPa at 3 pm), thus no sign of significant water stress. The value of average water potential at turgor loss pint achieved at -1.92 MPa, indicated a phenomenon of moderate drought resistance species. Based on this results, the discussion emphasize the water relations of tripical forest plants and the important of plant water relations information in the managment of National Parks and other reserved biosphere, where plants as a main components. It is suggested to enlarge such this study (incluiding seasonal fluctuations of plant water relations and covering wider forest species), under the light of Natinal park and other biosphere sities managment.
PENGARUH PEMBERIAN ELISITOR JAMUR Phytium aphanidermatum (Edson) Fitzp. TERHADAP KANDUNGAN AJMALISIN PADA KULTUR KALUS BERAKAR Catharanthus roseus (L.) G. Don Aprianita Aprianita; Esyanti RR; AH Siregar
BERITA BIOLOGI Vol 6, No 4 (2003)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v6i4.3448

Abstract

An experiment to study the effect of ellicitor derived from Phytium aphanidermatum On ajmalicine content of Catharanthus roseus root- callus culture has been conducted. Root-callus was induced  from left segment, and grew optimally on Zenk medium with the addition of  10-7 M BAP and 10-5 M NAA. Root-callus was subcultured three times, and followed with elicitation with elicitor derived from autoclaved  P. aphanidermatum. The concentration tested were 0.50, 0.5, 1.0 and 5.0 mg DW/ml, and harcested at 0,18,36 and 72 hours. The ajmalicine was analyzed qualitatively and quantitatively by using High Performance Liquid Chromatography (HPLC). The result showed that ajmalicine content was influenced significantly by concentration of ellicitor and harvesting time. A significant increase of ajamalicine content (153.29 ± 1.61 mg/g DW) was achieved by additon of 1 mg Dw/ml ellicitor after 36 hours incubation. 
ISOLASI DAN SELEKSI JAMUR PENDEGRADASI SENYAWA BENSONITRIL YS Subowo; Bambang Sunarko; Indrawati Gandjar
BERITA BIOLOGI Vol 6, No 4 (2003)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v6i4.3453

Abstract

A study on Isolation and selection of benzonitrile degrading fungi were conducted. The aim of this study was to obtain fungi that high potentially on degrading nitrile compounds. Microbial sources were derivat from industrial wastes. fungi-infecting plantand fungi grown on decayed wood. Eighteen isolates were isolated from those samples. Five isolates were capable to grow on benzonitrile. Isolate-AVI which was identified as Fusarium oxysporum was capable of degrading 0.15% (v/v) benzonitrile.
KARAKTERISASI ENZIM PENDEGRADASI SENYAWA ASETONITRIL DALAM SEL Fusiarum solani AIII2 YS Subowo
BERITA BIOLOGI Vol 6, No 4 (2003)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v6i4.3458

Abstract

An acetonitrlie degrading-enzyme of Fusarium solani AIII2 from whole cells were studied. Decrease of acetonitrlie concentration and formation of it's degraded products were determined by gas chromatography. Ammonia analysis was done by Nessler's method. Fusarium solani AIII2 degraded 1% (v/v) acetonitrlie and produced acetic acid and ammonia. Acetonitrlie was degraded by nitrilase, with a rate of 0.903 mol min. mg dry weight cells. Maximum nitrilase activity of fusarium solani AIII2 was observed.
KEANEKARAGAMAN ARTHROPODA TANAH DI LANTAI HUTAN KAWASAN HULU SUNGAI KATINGAN KALIMANTAN TENGAH Cahyo Rahmadi; Yayuk R Suhardjono
BERITA BIOLOGI Vol 6, No 4 (2003)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v6i4.3449

Abstract

The Study of soil arthropoda diversity in Upper Katingan River area was done in five different forest types i.e. 4 natural forest (With different vegetation) and one industrial tree plantation (HTI). The research site belongs to the PT Dwima Jaya Utama a consession timber in Central Kalimantan. Pitfall traps and soil litter samples were used to collect the arthropods and insect specimen. Twenty pitfall traps were set along 2 transect with 100 m length in each site and were operated forfour day. five soil-litter smaples of two litter were collected from each site. Soil-litter samples were examined in Berlese funnels for two weeks to sort the animals from debris. Identification was done based on the morphological appearce (morphospecies) and some taxon was determined up to order and/of family name. The results show that the most dominated taxon were collembola consit of 103 species from 13 families, and Coleoptera (33 species, 11 families). The most dominated taxon from Class Arachnid was Acarina (11 taxon) and Araneae (7 families). The Kilometer 33 (PUP) area has the highest species richness and abundance, whereas the Industrial Tree Plantation (HTI) has the lowest one of soil Arthropods. Based on the role of Arthropods on the forest floor, the most dominated group was decomposer and followed by predator and phytophagous. The unknown group mostly was consist of juveniles.

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