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INDONESIA
BERITA BIOLOGI
Published by Lembaga Ilmu Pengetahuan Indonesia
ISSN : -     EISSN : -     DOI : -
Core Subject : Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology
Arjuna Subject : -
Articles 30 Documents
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Search results for , issue "Vol 8, No 6 (2007)" : 30 Documents clear
PRODUKSI KITINASE EKSTRASELULER Aspergillus rugulosus 501 SECARA OPTIMAL PADA MEDIA CAIR Widhyastuti, Nunuk
BERITA BIOLOGI Vol 8, No 6 (2007)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (407.982 KB) | DOI: 10.14203/beritabiologi.v8i6.839

Abstract

Chitinases hydrolyzing chitin are produced by various organisms and their physiological functions depend on their sources.Chitinases and its hydrolysis products have a broad range of applications. Increasing commercial interest in chitinase and its products has led to the need of inexpensive, reliable sources of active and stable chitinase preparations.The activity of chitinase produced by Aspergillus rugulosus 501 in liquid culture and the parameters that controlled the enzyme synthesis were studied to find optimal conditions for the enzyme production. Hydrolysis products of chitinase were detected by Reissig method. The results showed that fungal chitinase activity in media containing organic nitrogen and phosphate was higher than that in media containing trace element, organic and anorganic nitrogen but lack of phosphate. The maximum enzyme activity reached in the second day incubation.Chitinase production was affected by the initial pH of media, chitin consentration and source of nitrogen, while the consentrasion of inoculum used in this study did not affect enzyme production. High enzyme activity was found in media with initial pH 5.0-6.5. When using different consentration of colloidal chitin, chitinase activities were also found high in concentration from 0.75% tol .5%. The organic nitrogen sources gave the better results compared to the anorganic ones.
KAJIAN ILMIAH IKAN PELANGI {Marosatherina ladigesi (Ahl 1936)} FAUNA ENDEMIK SULAWESI Hadiaty, Renny Kurnia
BERITA BIOLOGI Vol 8, No 6 (2007)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (414.276 KB) | DOI: 10.14203/beritabiologi.v8i6.827

Abstract

Marosatherina ladigesi is one of the famous rainbow fish species from Sulawesi.This endemic fish species from Sulawesi is one of the Indonesian export commodity since more than 30 years ago.All of the export specimens come from the wild habitat.The anxiousness of the extinction of this species stated in the redlist of IUCN since 1994. Two field work of Maros Karst Project conducted in 2006, 2007 and an international expedition in 2007 showed the decreasing population of this species.The results of the three field trips showed the difficulties to get M. ladigesi in the streams.Taxonomical status and classification, coloration, sex dimorphism and distribution discussed.
EFEKTOKSISITAS BIJI KEMALAKIAN (Croton tiglium) TERHADAP MENCIT DAN ANJING Yuningsih, Yuningsih; R, Damayanti
BERITA BIOLOGI Vol 8, No 6 (2007)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (292.302 KB) | DOI: 10.14203/beritabiologi.v8i6.835

Abstract

Study in attempt to substitute commercial imported strychnine for elimination wild dogs in rabies disease program with Kimalakian (Croton tiglium) seeds has been conducted. This research was aimed to examine the destructive effect of croton seed petroleum extract (oil) in mice. There were 50 mice divided into 5 treatment groups. Each group was given (orally) 0.05, 0.10, 0.20, 0.40 and 0.80 ml oil/mice respectively and 1 group (10 mice) was blank. After the animal died necropsy was performed and gross pathological and hystopathological features were observed. The results showed that LD!0: 1.66 ml of oil/kg b.w. on mice. Furthermore, it was applied to dogs (b.w. 3kg) with dose: 2.5 ml, 5.0 ml and 10 ml oil/dog respectively. All dogs became hyperactive within few minutes, then dead after dosing 5.0 and 10.0 ml oil. Gross lesion in mices and dogs showed general congestion and hemorrhage in the lung, heart and liver. Histopathologically, the gastric mucosa was atropic resulted from gastric malfunction as leading to death.
MUTASI STRUCTURAL INTRON trnL (UAA) PADA SUKU MERANTI-MERANTIAN (DIPTEROCARPACEAE) Yulita, Kusumadewi Sri
BERITA BIOLOGI Vol 8, No 6 (2007)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (850.353 KB) | DOI: 10.14203/beritabiologi.v8i6.823

Abstract

tRNA " UAA (trnL) intron have been widely utilised for phylogenetic reconstruction in angiosperm families. Two main helical elements of secondary structure of trnL intron (stem loop regions P6 and P8) contained the most variable sequences across taxa.Detailed examination on the insertion-deletion events in trnL intron sequences of 110 species of Dipterocarpaceae has identified 4 stem loop structures within both loops.The first stemJoqp was found in P6 loop and three other were discovered in P8 loop.These structures featuring characteristic for genericJinfra-generic level depended upon which taxonomic classifications are foJJowed.One of these structures formed a large loop of 72 nucleotides and was a unique major stem loop in Dipterocarps family whose loop was the major structural mutation of trnL intron in this family.
PROTEIN TOKSININSEKTISIDAL DARI BAKTERI PATOGEN SERANGGA Photorhabdus luminescens HJ Akhdiya, Alina; Pratiwi, Etty; Samudra, I Made
BERITA BIOLOGI Vol 8, No 6 (2007)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (377.996 KB) | DOI: 10.14203/beritabiologi.v8i6.828

Abstract

Photorhabdus luminescens HJ is an entomopathogenic bacterium that has a high toxicity against Tenebrio molitor larvae.Toxicity assay of crude extra cellular protein precipitated using ammonium sulphate showed that the highest toxin activity was found in 70 % saturation. Purification of the toxin using Hi Prep 16/60 Sephacryl S-200 HR column exhibited one fraction of toxic protein and three fractions of non-toxic protein. Mortality of T. molitor larvae treated with 19.2 nanogram of toxic fraction was up to 80%. Denatured protein analysis using sodium dodecyl sulphate-polyacrylamide gel electrophoresis revealed that the toxic fraction was composed of three proteins, which were 19.5, 42, and 66 kDa respectively. Based on toxin activity bioassay, this toxin type was an injectable toxin and presumably classified as Mcf toxin.
NEMATODA PADA TIKUS SUKU MURIDAE DAN POLA INFEKSINYA DITAMAN NASIONAL LORE LINDU, SULAWESI TENGAH Endang Purwaningsih; Kartika Dewi
BERITA BIOLOGI Vol 8, No 6 (2007)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v8i6.831

Abstract

The study of the patterns of nematode infection on rodents in Lore Lindu, Central Sulawesi was carried out. A total of 52 rodents were examined. Of these rodents 37 (71.15 per cent) were found to harbour nematode parasites and 15 (28.85 per cent) were free.There were found 10 species of nematode: Subulura andersoni, Syphacia muris, Heterakis spumosa, Cyclodontostomum purvisi,Hepatojarakus malayae, Molinacuaria indonesiensis, Protospirura muris,Rictularia tani, Trichuris muris dan Nippostrongylus muris that found in intestine, stomach, cecum and liver.The kind of infections were single (17.31 per cent) and association (53.84 per cent).The highest prevalence was 5. andersoni (44.23 per cent). Each of species nematodes had a specific habitat in their hosts. The greatest number of habitat that species of nematodes found was stomach (all of the species nematodes were found at the stomach).
KRIOPRESERVASI TANAMAN PURWOCENG {Pimpinella pruatjan Molk.) DENGAN TEKNIK VITRIFIKASI I Roostika; I Darwati; R Megia
BERITA BIOLOGI Vol 8, No 6 (2007)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v8i6.821

Abstract

Pruatjan (Pimpinellapruatjan Molk.) is an Indonesian endangered medicinal plant that included in Appendix I based on CITES. Therefore it is a highly protected species. To avoid extinction of this plant, it is very important to conserve the plant. In vitro conservation is more suitable since this plant is difficult to be cultivated outside of its habitat. Cryopreservation technique may conserve this material for a long-term period. The objectives of this research were to find optimized treatments for pre culture, loading, and dehydration on cryopreservation of pruatjan. The research was conducted at Tissue Culture Laboratory in Indonesian Center for Agricultural Biotechnology and Genetic Resources Research and Development, started from May to November 2007. Pre culture was conducted using DKW basal media that added by sucrose at the level of 0.3,0.4, and 0.5M for one and three days incubation. Loading was conducted in DKW basal media containing 2M glycerol and 0.4M sucrose for 15,30, and 45 minutes duration time. Dehydration was conducted in several cryoprotectants, namely PVS1 (22% glycerol + 13% propy lene glycol + 13% etylene glycol + 6% DMSO + 3% sucrose), PVS2 (30% glycerol + 15% etylene glycol + 15% DMSO + 0,4M sucrose), PVS3 (50% glycerol + 50% sucrose), and PVS4 (35% glycerol + 20% etylene glycol + sucrose 0.6M). Result showed that pruatjan could be preserved through cryopreservation by vitrification method. The best pre culture was using 0.3 M sucrose for one day, the best loading was 30 minutes, while the best cryoprotectant was PVS2 with 90% success before freezing and 40% after freezing. The success may be improved by applying pre growth treatment, optimizing temperature of thawing, modification of recovery media and incubation condition.
PRODUKSI KITINASE EKSTRASELULER Aspergillus rugulosus 501 SECARA OPTIMAL PADA MEDIA CAIR Nunuk Widhyastuti
BERITA BIOLOGI Vol 8, No 6 (2007)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v8i6.839

Abstract

Chitinases hydrolyzing chitin are produced by various organisms and their physiological functions depend on their sources.Chitinases and its hydrolysis products have a broad range of applications. Increasing commercial interest in chitinase and its products has led to the need of inexpensive, reliable sources of active and stable chitinase preparations.The activity of chitinase produced by Aspergillus rugulosus 501 in liquid culture and the parameters that controlled the enzyme synthesis were studied to find optimal conditions for the enzyme production. Hydrolysis products of chitinase were detected by Reissig method. The results showed that fungal chitinase activity in media containing organic nitrogen and phosphate was higher than that in media containing trace element, organic and anorganic nitrogen but lack of phosphate. The maximum enzyme activity reached in the second day incubation.Chitinase production was affected by the initial pH of media, chitin consentration and source of nitrogen, while the consentrasion of inoculum used in this study did not affect enzyme production. High enzyme activity was found in media with initial pH 5.0-6.5. When using different consentration of colloidal chitin, chitinase activities were also found high in concentration from 0.75% tol .5%. The organic nitrogen sources gave the better results compared to the anorganic ones.
KAJIAN ILMIAH IKAN PELANGI {Marosatherina ladigesi (Ahl 1936)} FAUNA ENDEMIK SULAWESI Renny Kurnia Hadiaty
BERITA BIOLOGI Vol 8, No 6 (2007)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v8i6.827

Abstract

Marosatherina ladigesi is one of the famous rainbow fish species from Sulawesi.This endemic fish species from Sulawesi is one of the Indonesian export commodity since more than 30 years ago.All of the export specimens come from the wild habitat.The anxiousness of the extinction of this species stated in the redlist of IUCN since 1994. Two field work of Maros Karst Project conducted in 2006, 2007 and an international expedition in 2007 showed the decreasing population of this species.The results of the three field trips showed the difficulties to get M. ladigesi in the streams.Taxonomical status and classification, coloration, sex dimorphism and distribution discussed.
EFEKTOKSISITAS BIJI KEMALAKIAN (Croton tiglium) TERHADAP MENCIT DAN ANJING Yuningsih Yuningsih; Damayanti R
BERITA BIOLOGI Vol 8, No 6 (2007)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v8i6.835

Abstract

Study in attempt to substitute commercial imported strychnine for elimination wild dogs in rabies disease program with Kimalakian (Croton tiglium) seeds has been conducted. This research was aimed to examine the destructive effect of croton seed petroleum extract (oil) in mice. There were 50 mice divided into 5 treatment groups. Each group was given (orally) 0.05, 0.10, 0.20, 0.40 and 0.80 ml oil/mice respectively and 1 group (10 mice) was blank. After the animal died necropsy was performed and gross pathological and hystopathological features were observed. The results showed that LD!0: 1.66 ml of oil/kg b.w. on mice. Furthermore, it was applied to dogs (b.w. 3kg) with dose: 2.5 ml, 5.0 ml and 10 ml oil/dog respectively. All dogs became hyperactive within few minutes, then dead after dosing 5.0 and 10.0 ml oil. Gross lesion in mices and dogs showed general congestion and hemorrhage in the lung, heart and liver. Histopathologically, the gastric mucosa was atropic resulted from gastric malfunction as leading to death.

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