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BERITA BIOLOGI
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Articles 30 Documents
Search results for , issue "Vol 9, No 2 (2008)" : 30 Documents clear
DIVERSITAS DAN PROFIL METABOLIT SEKUNDER JAMUR ENDOFIT YANG DIISOLASI DARI TUMBUHAN GAMBIR (Uncaria gambler) SERTA AKTIVITAS BIOLOGISNYA SEBAGAI ANTIBAKTERI Yuliasri Jamal; Muhamad Ilyas; Atit Kanti; Andria Agusta
BERITA BIOLOGI Vol 9, No 2 (2008)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v9i2.2023

Abstract

Five kinds of endophytic filamentous fungi i.e. Pestalotiopsis sp. GNC, Fusarium sp. GNC-A, Fusahum sp. GNC-B, Pestalotiopsis sp. GUC and Fusarium sp. GUC were isolated from surface sterilized of steems of gambir nasi and gambir udang (Uncaria gambier Roxb.) collected from Rengat, Riau Province,Indonesia. Identification of the endophytic fungi were done through morphologically observations and chemotaxonomically approach. In an antibacterial assays, the ethyl acetate extract of the Pestalotiopsis sp.GNC cultures showed their activity against pathogenic bacteria, Eschericia coli, Staphylococcus aureus and Bacillus sublilis.Beside the above three pathogenic bacteria, the ethyl acetate extract of Fusarium sp. GNC-B culture was also active against
KEANEKARAGAMAN JENIS BEGONIA (Begoniaceae) LIAR DI JAWA BARAT Deden Girmansyah
BERITA BIOLOGI Vol 9, No 2 (2008)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v9i2.2028

Abstract

A taxonomic study of the wild genera of Begonia Blume {Begoniaceae) in West Java was conducted based on morphological characters. Nine known species (Begonia areolata Miq., Begonia isoptera Dryander, Begonia lepida Blume, B. longifolia Blume, B. multangula Blume, B. muricata Blume, B. repanda Blume, B. robusta Blume and B. tenuifolia Dryander) and one new species Begonia sp. was discovered. An identification key and information about potential uses of West Java's Begonia are described. Beside as ornamental plants, Begonia can be used for medicinal plant such as B. isoptera for spleen diseases.
KILAS BALIK PENELITIAN KROMOSOM PALEM INDONESIA Joko Ridho Witono
BERITA BIOLOGI Vol 9, No 2 (2008)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v9i2.2019

Abstract

There are 525 native palm species to Indonesia.Chromosome numbers of 61 Indonesian palm species have been reported,unfortunately, not all species were presented by their karyotype. Re-observation of palm chromosomes is needed, because mis identification of particular species and uncorrect chromosome counts might be happen in the past. Chromosome characteristics of some families, such as Poaceae and Onagraceae were proved useful for classification.On palms, almost all species within similar genera were known had similar chromosome numbers and different in chromosome forms and sizes.Trends to employ chromosome characteristics for palm classification is possible.Since, chromosome information on palm is limited,chromosome research has great challenge to do in the future.
ISOLASI DAN IDENTIFIKASI SENYAWA ANTIBAKTERI MINYAK ATSIRI DAUN KEMBANG BULAN {Tithonia diversifolia (Hemsley) A. Gray} Hartati Soetjipto; Lusiawati Dewi; Sentot Adi Prayitno
BERITA BIOLOGI Vol 9, No 2 (2008)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v9i2.2024

Abstract

Isolation and identification of chemical compounds and antibacterial compounds of the essential oil of Kembang bulan or Japanese sunflower {Tithonia diversifolia (Hemsley) A. Gray} leaves showed that the essential oil contain 29 components. The main components were caryophyllene (27.76%), nerolidol (21.81%),caryophyllene oxide (7.06%), copaene (6.41%) and bicylogermacrene (4.90%). The oil was screened for their antibacterial activity through bioautography method. Two antibacterial spots which had Rf 0.49 and Rf 0.61were obtained. The spot with Rf 0.49 was a mixture of 21 compounds with main compounds were heneicosane, nonacosane and tetratetracontane, while the spot with Rf 0.61 consisted of 22 compounds, which the main compound was nerolidol.
VAKSINASI DINI Bordetella bronchiseptica PADA ANAK BABI MENCEGAH KERUSAKAN SEL-SEL EPITEL BERBULU GETAR PADA MUKOSA SALURAN NAFAS BAGIAN ATAS Siti Chotiah
BERITA BIOLOGI Vol 9, No 2 (2008)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v9i2.2029

Abstract

The upper respiratory tract is lined with ciliated epithelium or brush border that functions as the primary filter to the respiratory tissue. Epithelial trauma caused by neonatal bordetellosis serves as the predisposing factor for other respiratory diseases in growing pig. The aim of this research was to evaluate the effectiveness life nonpathogenic Bordetella Bronchiseptica vaccine that used in sucking piglets in Indonesia, to prevent the damage of ciliated epithelium. Twenty four sucking piglets 2 to 3 day-old were divided into treatment group containing fourteen and nontreatment group containing ten. Each sucking piglet of treatment group was vaccinated with life nonpathogenic Bordetella Bronchiseptica vaccine by intra nasal with 0.5 ml/nostril dose containing more than72.0xl0 CFU/ml bacteria. Three weeks post vaccination 10 of those treatment group and 6 from nontreatment group were challenged against local isolate B. bronchiseptica BS9 (BCC2455) toxigenic with 4.2xlO'CFU/ml dose. The clinical signs, reisolation of bacteria, and histopathological changes were observed. The result showed that 3 of 10, 5 of 6, 0 of 4, and 3 of 4 treatment and challenge sucking piglet group, nontreatment and challenge sucking piglet group, treatment and not challenge sucking piglet group,and nontreatment and not challenge sucking piglet group were found lost of cilia from most of the ciliated epithelial cells of nasal mucosa respectively. Three of 10, 4 of 6, 1 of 4, and 4 of 4 treatment and challenge sucking piglet group, nontreatment and challenge sucking piglet group, treatment and not challenge sucking piglet group, and nontreatment and not challenge sucking piglet group were found epithelial cells desquamation of nasal mucosa respectively. The results indicated that life nonpathogenic Bordetella Bronchiseptica vaccine could give 70 % protection against infection of B. bronchiseptica local isolate BS9 (BCC245) toxigenic infection and 75% wild type isolate present at the farm used for this research.
PEMANFAATAN KONSORSIUM BAKTERI LOKAL UNTUK BIOREMEDIASI LIMBAH TEKSTIL MENGGUNAKAN SISTEM KOMBINASI ANAEROBIK-AEROBIK I Dewa K Sastrawidana; Bibiana W Lay; Anas Miftah Fauzi; Dwi Andreas Santosa
BERITA BIOLOGI Vol 9, No 2 (2008)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v9i2.2020

Abstract

The objective of this research is to study the potential use microorganisms which are identified as Aeromonas sp., Pseudomonas $p., Flavobaclerium sp., Plesiomonas sp. and Vibrio sp. Five bacteria strains from sludge of Badung river were identified as Vibrio sp. and Plesiomonas sp. Two anaerobic-aerobic reactors were operated to treat textile waste water. Each reactor contained vulcanic stone to increase spesific surface of media for attachment of bacteria.Bacteria consortia used for anaerobic process consist of Aeromonas sp. (ML6), Aeromonas sp. (ML14), Aeromonas sp. 9ML24), Pseudomonas sp. (ML8) and Flavobacterium sp. (ML20). Whereas, bacteria consortia for aerobic process consist of Plesiomonas sp. (SB1), Plesiomonas sp. (SB2), Vibrio sp.(SB1), Vibrio sp. (SB2) and Vibrio sp. (SB3). The system was operated for 3 day in each reactor. The result showed, biodegradation of textile waste water in combined anaerobic-aerobic system by attached growth process is potential for treatment of textile waste water.This technology is effective to decrease COD value up to 98.38%, BODS 93.90%, TDS 80.87%, TSS 87.50% and decolorization of textile dyes up to 95.57%.
KAJIAN FEKUNDITAS DAN DAYA TETAS TELUR IKAN BETUTU (Oxyeleotris marmorata) PADA WAD AH PEMIJAHAN YANG BERBEDA Sri Karyaningsih
BERITA BIOLOGI Vol 9, No 2 (2008)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v9i2.2025

Abstract

Assessment of fecundity and hatching rate of betutu fish - sand goby {Oxyeleotris marmorata) eggs has been done at the station rearing center in Ngrajek, Magelang Regency, Central Java. The fecundity and hatching rate of sand goby eggs can be used as measuring rod of fry. The result showed that the fecundity of rearing in fiber pond between 4.700 to 10.250 eggs with the hatching rate are 41.44% to 85.06%. The rearing in concrete pond between 5.400 tol4.000 eggs with the hatching rate are 43.20% to 90.00%. Statistical test on relationship indicates that no significant for fecundity and hatching rate about the rearing on different case. The relationship between the fecundity with the body length and the body weight is significant. The fecundity and hatching rate was under the influence of ovary weight, diet and hatchery environment.
PERKECAMBAHAN DAN VIGOR SEMAI Picrasma javanica Blume PADA BERB AGAI SUHU Hadi Sutarno; Ning Wikan Utami
BERITA BIOLOGI Vol 9, No 2 (2008)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v9i2.2030

Abstract

The successfull cultivation of useful plant largely depends on the quality of seeds, especially viability and germination vigor. Seed of Picrasma javanica Blume has potential use in the development of templates for new drugs, e. g. to treat malaria.For most species there is still little or no information about the suitable temperature regime for germination and seedling depelopment,therefore,it is interesting to study germination and seedling vigor of Picrasma javanica. The mature green were picked and cleaned before using for experiments.Germination was carried out in 24 compartments of a thermogradientbar apparatus.The temperature gradient extends from 4.8 to 41.6°C with 1.6°C steps from the first to the 24 th compartment. Twenty seeds were germinated in each compartment. Seeds were sown on 2 layers of filter-paper strips.Everyday for 77 days during germination the germinating seeds were recorded.For hilling experiments those seeds from compartment number 3 to number 13 of thermogradientbar apparatus were than removed to incubator 32°C for 28 days. On the seedling vigor, all germinating seed from incubator were removed for replanting in growth room with sand medium. The seedling establishment capacity in the different thermal regions can thus be identified. The results showed that seeds attained 50% germination after 43 days when placed at the gradientbar 22.4°C to 41.6°C with optimum temperature 36.8°C. A 28 days temperatuture pretreatment ranging gradually from 4.8°C up to 20.8°C has promoted germination significantly. Mechanism of pretreatment on low and high temperature was discussed.
SISTEM PENYERBUKAN ALTERNATIF Talinum triangulare Willd.: EFEK PERLAKUAN PENYERBUKAN PAD A AKTIFITAS BUNGA DAN PEMBENTUKAN BUI Erlin Rachman
BERITA BIOLOGI Vol 9, No 2 (2008)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v9i2.2021

Abstract

Eight pollination treatments were executed on Talinum triangulare Willd. to observe alternative pollination systems and its effect on some flower activities and seed setting. The treatments can be grouped to autogamy and allogamy or geitonogamy.Flowers simultaneously were treated by the eight treatments day by day for two weeks in a month with three replications.Parameters observed were fruit set, anthesis duration, seed number per capsule and presence of stigmatic curvature. The result showed that the plant species was evidently flexible in pollination systems but the plant was outcrossing species with highest seed number (58.73 ± 5.95) per capsule and did not show stigmatic lobe curvature. The second and the third highest seed number per capsule were given by natural pollination with or without anther removal and with or without artificial polination (50.5 ± 2,87 and 49.0 ± 1) and this treatments also did not showed stigmatic lobe curvature. Articial pollination with or without flower bagging, withor without anther removal and with or without natural pollination generally gave lower seed number per capsule 40.67 ± 5.69,32.67 ± 10.95, 43.67 ± 9.5) except spontan autogamy(49.29 ± 10.14) and some of this treatments showed sigmatic lobe curvature.Stigmatic lobe curvature, therefore, was occured when no pollen occupation on it until prior to naturally flower reclose. Flower would be abscised when no artificial or natural pollination was happened but its stigma was curved before shed. Primary pollination mechanism in the plant was cross polllination (allogamy) but the plant is enough flexible in pollination systems. Autogamy especially spontan autogamy seem to be alternative pollination mechanism when no natural polinator visit.
KEANEKARAGAMAN DAN DAYA DEGRADASI SELULOSA JAMUR TANAH DIHUTAN BEKAS TERBAKAR WANARISET-SEMBOJA, KALIMANTAN TIMUR Suciatmih Suciatmih
BERITA BIOLOGI Vol 9, No 2 (2008)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v9i2.2026

Abstract

In order to know the effect of isolation method on the occurrence and capability of soil fungi to degrade cellulose,a study was conducted in postburning forest in Wanariset-Semboja, East Kalimantan. Soil fungi were isolated using three isolation methods:incubation at 45° C, treatment with 50 % ethanol for 15 minutes, and heat treatment at 70° C for 15 minutes.Plates for heat incubation and for other methods were incubated at 45° C and 27° C for three days, respectively. Cellulose degradation test of isolated fungi was examined using Carboxy Methyl Cellulose (CMC) media. Results showed that isolation method affected diversity and population of soil fungi. Heat treatment at 70° C for 15 minutes appeared to have highest diversity and population of soil fungi. Eupenicillium Javanicum var javanicum (van Veyma) Stolk & Scott, Talaromyces byssoclamydoides Stolk & Samson, T. flavus(Klocker) Stolk & Samson, T. stipitatus C.R. Benjamin, and Penicillium argillaceum Stolk et al. were dominant in postburning forest in Wanariset-Semboja, East Kalimantan. Twenty-one isolated fungi degraded cellulose.

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