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BERITA BIOLOGI
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Search results for , issue "Vol 9, No 5 (2009)" : 40 Documents clear
KONSEP JENIS PALEM: SEBUAH PENGANTAR Himmah Rustiami
BERITA BIOLOGI Vol 9, No 5 (2009)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v9i5.1982

Abstract

In recent decade, the needs of clear species concept for constructing monograph became more crucial. Trends on species concept have been more overwhelming nowadays because of the rigorous phylogenetic species concept based on the idea of the smallest unit sexually from a single population or based on the known lineages within taxa in regard with unique characters combination.This paper would like to discuss further how is the palm species concept and their distribution pattern in West Malesia using case study on the Nypa species concept.
KONDISI OPTIMUM FUSI PROTOPLAS ANTARA JAMUR TIRAM PUTIH {Peurotus floridae) DAN JAMUR TIRAM COKLAT {Pleurotus cystidiosusy Ira N Djajanegara; Korri El-khobar
BERITA BIOLOGI Vol 9, No 5 (2009)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v9i5.1997

Abstract

Genetic crossing of white oyster mushroom to introduce longer storage life trait can only be done within individuals in this particular species. However, longer storage life trait is possessed by brown oyster mushroom (Pleurotus cystidiosus) which is other species within this genus. Thefeore, protoplast fusion between white oyster mushroom (Peurotus floridae) and brown oyster mushroom (Pleurotus cystidiosus) was conducted to hopefully obtain an oyster mushroom strain that has higher production and longer storage life. Protoplast fusion was done by isolating protoplast from 5-days old monokaryotic mycelia grown in PDB. As much as 3.15 x 10 protoplasts/ml were harvested using mixture of cellulase Onozuka R-10 (1%) and macerozyme R-10 (1%) from brown oyster mushroom with 80.61% viability. Similarly, 3.71 x 10 protoplasts/ml were harvested using lysing enzyme (2%)from brown oyster mushroom with 83.68% viability. Protoplast fusion were conducted using 0% (control), 30%, 40% and 50% of PEG6000. Fusion periods were done at 10, 20 and 30 minutes. The candidate fusants were then screened using MRM (minimum regeneration media) media. Based on this experiment, the optimum conditions for protoplast fusion is 10 minutes incubation using 40% PEG6000 that yielded 121 colonies grown on MRM media as candidate fusants.
IDENTIFIKASI MOLEKULAR ISOLAT KAPANG PENGHASIL ß-GLUCAN BERDASARKAN DAERAH INTERNAL TRANSCRIBED SPACER (ITS) Yoice Srikandace; Ines Irene Caterina A; Wibowo Mangunwardoyo
BERITA BIOLOGI Vol 9, No 5 (2009)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v9i5.1987

Abstract

Research was conducted to identify the fungal isolate G.23 which produces b-glucan from the Biopharmacy Laboratorium's collection.Indonesian Institute of Sciences (LIP1) based on the sequence of the Internal Transcribed Spacer (ITS) regions.DNA was isolated from mycelia and the ITS region was amplified by Polymerase Chain Reaction (PCR) with ITS1 and ITS4 primers. The PCR product was purified using the QIAquick PCR Purification kit (Qiagen). BigDye terminator cycle sequencing Ready Reaction Kit (Perkin Elmer Applied Biosystem) was used and the product was purified with the AutoSEQ G-50 Kit (Qiagen).The sequence obtained analysed using Basic Local Alignment Search Tool nucleotide (BLAST)n homology search.The BLASTn result showed that the fungal isolate G.23 belongs to the genus Aspergillus. Taxa closely related to this isolate were Aspergillus elegans,A. ochraceus and A.sclerotiorum with 96% sequence homology.ClustalX was used for sequence-alignment. Phylogenetic analysis was constructed using the Neighbour Joining (NJ) method with Kimura two parameters. The phylogenetic tree obtained showed that fungal isolate G.23 separated from A. elegans, A. ochraceus and A. sclerotiorum which indicated that fungal G.23 belonged to a different spesies.Morphological observation on culture and microscopic appearance of the fungal isolate G.23 supported that this isolate differs from A. elegans, A. ochraceus and A. sclerotiorum.
EKOLOGI DAN PERSEBARAN GEWANG (Corypha utan Lamk.) DI SAVANA TIMOR, NUSA TENGGARA TIMUR Tukirin Partomihardjo; BP Naiola
BERITA BIOLOGI Vol 9, No 5 (2009)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v9i5.2002

Abstract

A study of the distribution and ecology of Corypha utan Lamk. (gewang) within the natural habitat of savana ecosystem at Usapisonba'i village area, Kecamatan Nekamese, Kabupaten/Regency Kupang, East Lesser Sunda Islands was conducted to gain an understanding factors controlling distribution and dynamics populations of the species. Three plots with total of 2.1 ha were established at different sites. Reconnaissence throughout the study area revealed that gewang grows in the lowlands below 200 m altitude, but away from the immmediate vicinity of the sea. Populations were studied within different savana types. It was concluded that gewang appears to be relatively associated to the open areas. The abundance of gewang within the study area formed the main savana vegetation as savanna gewang type. Distribution pattern of gewang within the study area tend to be concentrated at the lower part of the plots in particular at relatively flat areas. Examination of seedling and sapling densities in relation to parent trees revealed a large number of propagules fallings to disperse, and in consequence tendencies toward dumb distributions. The seedlings tend to grow under dead parent tree and or at flat of lower areas. Based on the population structure, gewang revealed has good regeneration strategy with high population of younger stages.
ANALISA VEGETASI HUTAN RIPARIAN DATARAN RENDAH DITEPI SUNGAI NGGENG, TAMAN NASIONAL KAYAN MENTARANG, KALIMANTAN TIMUR Purwaningsih Purwaningsih
BERITA BIOLOGI Vol 9, No 5 (2009)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v9i5.1992

Abstract

Study on the riparian forest of Nggeng River bank at Kayan Mentarang National Park was carried out with quadrate method. It would cover species composition and vegetation structure. The results of a floristic inventory of trees with DBH e" 10 cm in a lowland riparian forest of Nggeng river side show that 106 species, consist of 53 genera and 29 families in the plot of 2 hectare sampled. The two leading families in terms of number of species were Dipterocarpaceae and Myrtaceae while according to the total sum of importance values were Dipterocarpaceae and Fabaceae. The common species in two plots were Saraca hulletii, Parashorea parviflora, Dipterocarpus oblongifolius, Castanopsis motleyana, and Dryobalanops lanceolata. The diversity of the river-side were lower than the other forest in Kalimantan and in north is more rich of species than south plot. The forest type on the river side were shown mixed dipterocarp forest, because the forest dominated by dipterocarps species. Forest structure on the sides of Nggeng River were shown there were some emergent trees, they are up to 45 m tall and in diameter >100 cm. Some emergent trees in the location were such as Parashorea parviflora, Dipterocarpus oblongifolius and Shorea spp. Lists on the most 10 leading family trees, 10 leading family saplings, density, frequency and important values of each inventory plants species are provided.
KINERJA Saccharomyces cerevisiae REKOMBINAN [GLO1] DALAM PROSES SIMULTAN HIDROLISIS PATI DAN FERMENTASI UNTUK PRODUKSI BIOETANOL Afaf Baktir; Nur Cholifah; Sri Sumarsih
BERITA BIOLOGI Vol 9, No 5 (2009)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v9i5.1983

Abstract

Recent development in fermentations for bioethanol production were focused three factors, i.e. abundance and cheap substrates,superior yeast fermenting the substrates, and simultaneous saccharification and fermentation (SSF) technology.Nowadays national and world bioethanol production still depend on sugar cane and starchy materials.This research aims to determinate the optimum simultaneous saccharification and fermentation (SSF) conditions to identify the performance of local strain Saccharomyces cerevisiae recombinant [GLO1] in the producing bioethanol from starch.The optimum conditions for SSF process are in a media composition containing glucose 2% (w/v), starch 5% and at aeration rate 50 rpm.At these optimum conditions Saccharomyces cerevisiae recombinant [GLO1] produce 25.36% (v/v) bioethanol at day-20 of the fermentation process design.
INTERSPECIFIC ASSOCIATION PATTERNS AND EDAPHlC FACTORS' INFLUENCES: A CASE STUDY OF Orania regalis Zippelius IN WAIGEO ISLAND, WEST PAPUA [Pola Asosiasi Antarspesies dan Pengaruh Faktor Edafik: Studi Kasus Orania regalis Zippelius di Pulau Waigeo, Papua Barat] Didik Widyatmoko
BERITA BIOLOGI Vol 9, No 5 (2009)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v9i5.1998

Abstract

Tropical plant community ecology is often assumed to be driven largely by stochastic disturbance, regeneration and demographic processes. The influence and importance of physical and biotic components are rarely taken into account. Consequently, scientific findings in this field are very limited. Tropical soils are in fact more diverse than usually is recognized. This study was conducted to test hypotheses about the importance and influence of edaphic parameters and association patterns in determining the abundance and distribution of the Papuasian palm Orania regalis Zippelius and its co-occurrence with other plant species. The results showed that a number of interrelating edaphic factors, in combination, appeared to explain the abundance and occurrence of O. regalis. This palm showed a preference for wet, but well-drained soils, with high magnesium (Mg ) and calcium (Ca *)contents. The three largest colonies occurred in sites where Mg * content was high. On the other hand, high alkaline concentrations corresponded to the absence of the palm. Eight of 14 tropical plant species were positively associated while six species were negatively associated with O. regalis. For five species (Licuala gramnifolia, Tabernaemontana aurantiaca, Intsia bijuga, Vatica rassak,and Palaquium obovatum), the association with O. regalis was strong, as indicated by their Ochiai indices (>0.5).The palms L. gramnifolia and Sommieria leucophylla appeared to have similar ecological preferences and habitat requirements with O.regalis. The palm tended to occupy sites with lower C/N ratios and all known colonies occurred in habitats with average C/N values of less than 10. Based on the r-squared values, exchangeable Mg* appeared to have more influence on plant density and frequency than on basal area and canopy circle area. The exchangeable Ca * and C/N values showed a similar pattern to Mg contents.However, K ., Na ., Al and H contents did not show significant relationships with the palm abundance parameters. These findings suggest that edaphic variables and plant association patterns are important determinants of the abundance and occurrence of tropical plant species.
ABSORBSI GLUKOSA DAN SUKROSA SEBAGAI SUMBER KARBON UTAMA OLEH KOMUNITAS MPG PADA KONDISI ANAEROBIK-AEROBIK Dyah Supriyati
BERITA BIOLOGI Vol 9, No 5 (2009)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v9i5.1988

Abstract

To study the role of substrate on the establishment of glycogen accumulating organism (MPG), activated sludge originated from anaerobic-aerobic process was implemented on waste water treatment plant which was acclimated with glucose and sucrose.Both susbtrates were effectively utilized by microbial communities during anaerobic condition. Those absorbed substances were converted to glycogen.Glycogen was also used as energy generating process as indicated by a decrease of glycogen. The dominancy of glycogen accumulating organism suppresses the polyphosphate accumulating organism as indicated by no significant release of orthosphosphate during anaerobic condition.
SISTEM SOSIAL JANTAN MONYET HITAM SULAWESI (Macaca nigra) DI CAGAR ALAM TANGKOKO-BATUANGUS, SULAWESI UTARA Saroyo Saroyo
BERITA BIOLOGI Vol 9, No 5 (2009)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v9i5.1993

Abstract

A study on male social system of Sulawesi crested black macaque {Macaca nigra) has been conducted at Tangkoko-Batuangus Nature Reserve, North Sulawesi from January to October 2008.The aim of this research was to evaluate the male social system based on the social interaction among the males.The material was a habituated group called Rambo II that had 7 adult males and 19 adult females at early stage observation. Dominance hierarchy was determined with ad libitum sampling based on their results in agonistic interaction at 3 early-days of every month. The dynamics of the dominance hierarchy was traced during 10 months. Determination of dominance hierarchy linearity used Landau's index of linearity. Data of approaches among the males and their copulation frequency was collected with focal animal sampling.Approaches interaction data were analyzed for the direction of their approaches and the scoring of approachee responds that consisted of positive, neutral, negative, and without approaching.Results showed that during 10 months, there were 3 adult males that emigrated from Rambo II and one adult male that immigrated into the group.All dominance hierarchy had linearity indexes 1.0 that meant the hierarchies are perfect linear or linear and transitive.The a-male had highest frequency of copulation both with estrous and non-estrous females.For mid-rank male, on the other hand, the frequency was not always related to their rank. There was a tendency that dominate males approached subordinate males that was proved with their up/down index. Negative respond showed by subordinate males due to approaching by dominate ones was higher than the opposite. This result indicated that dominance played role in spatial controlling and freedom of interaction, for subordinate individual, the dominate presentating likely intimidated them. From those results, it can be concluded that the social system of male Sulawesi crested black macaque in not egalitarian and more despotic, different with the social system of the female that is considered as egalitarian.
PENINGKATAN PRODUKSI GAS HIDROGEN (H2 )DAN ETANOL PADA Bacillus pumilus DENGAN MUTASI MENGGUNAKAN Ethyl Methane Sulfonate (EMS) DAN SELEKSI DENGAN METODA PROTON SUICIDE Trismilah Trismilah; Mahyudin AR
BERITA BIOLOGI Vol 9, No 5 (2009)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v9i5.1984

Abstract

Mutation by using Ethyl Methane Sulphonate (EMS) was carried out in the study of the enhancement of H2 and ethanol production in Bacillus pumilus.Target mutant was selected by using proton suicide method. Bacterial suspensions was spread into agar minimal U medium containing 13 or, 14, 15 and 16 u.1 of Ethyl Methane Sulphonate (EMS) and incubated at 37 C for 3, or 4 , or 5, or 6 hours.The method of proton suicide was applied by the addition of equimolar of 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 75, 100, 125, 150, 175, 180, 185, 190, 195, 200 mM NaBr and NaBrO3.Triphenyl tetra chloride (TTC) was also added as indicator into agar plate in order to distinguish between wild type and mutants. Fermentation was carried out using glycerol complex medium.Hydrogen gas(H,) contain was determined by the replacement of NaCI solution in cylindrical glass and the ethanol was measured by gas chromatography. After mutation, several mutants were observed. In Mutant ( Asp8) which was obtained by treatment of 195 equimolar of NaBr and NaBrO,, production of ethanol and H2 were higher 10 fold and 1.13 fold, respectively compare to the wild type while acids production decreased. The data indicated that mutation might provoke metabolic alteration especially in acid production.

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