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Dental Journal (Majalah Kedokteran Gigi)
Published by Universitas Airlangga
ISSN : 19783728     EISSN : 24429740     DOI : -
Core Subject : Health,
Dentistry
The Dental Journal (Majalah Kedokteran Gigi) (e-ISSN:2442-9740; p-ISSN:1978-3728) is published by the Faculty of Dental Medicine, Universitas Airlangga. Its diciplinary focus is dental science and dental hygiene. The Dental Journal (Majalah Kedokteran Gigi) is published in English on a quarterly basis with each 50-60 page edition containing between nine and eleven scientific articles on research, study literature and case studies. Contributors to the Dental Journal (Majalah Kedokteran Gigi) included: dental researchers, dental practitioners, lecturers, and students drawn from Indonesia and a wide range of other countries.
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Articles 11 Documents
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Search results for , issue "Vol. 51 No. 3 (2018): September 2018" : 11 Documents clear
Cytotoxicity test of binjai leaf (Mangifera caesia) ethanol extract in relation to Vero cells Fifi Dwidhanti; Irham Taufiqurrahman; Bayu Indra Sukmana
Dental Journal (Majalah Kedokteran Gigi) Vol. 51 No. 3 (2018): September 2018
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (487.804 KB) | DOI: 10.20473/j.djmkg.v51.i3.p108-113

Abstract

Background: Binjai leaves (Mangifera caesia) constitute one part of a medicinal plant from South Borneo that contains potential anticancer and antioxidant flavonoids. Before using medicinal plants as adjuvant therapy material, a cytotoxicity test of a material extract needs to be conducted in order to establish the safety of natural ingredients that will be used in the production of medicinal products. Purpose: This research aimed to determine whether the ethanol extract of binjai leaves proved cytotoxic to Vero cells and determine the value of IC50 after the administering of ethanol extract of Binjai leaves by means of an MTT assay method. Methods: This research incorporated a true experimental method with posttest-only control design that consisted of ten groups. The Binjai leaf ethanol extract of varying concentrations was administered to eight groups, namely;1.25µg/mL, 62.5µg/mL, 125µg/mL, 250µg/mL, 500µg/mL, 1000µg/mL, 2000µg/mL and 4000µg/mL. The control groups consisted of two groups, one cell control group and one media control group. The cell viability percentage was calculated by an absorbent of ELISA reader. Results: The probit analysis result had an IC50 value of 2498.48µg/mL (IC50>1000µg/mL constituted a non-toxic category). Conclusion: Ethanol extract of Binjai leaves is not cytotoxic to Vero cells as shown by an assay MTT method which produced an IC50 value of 2498.48µg/mL.

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