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I G. Made Krisna Erawan
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Animal Hospital, Faculty of Veterinary Medecine Building, Udayana University, 2nd Floor, Jalan Raya Sesetan, Gang Markisa No 6, Banjar Gaduh, Sesetan, Denpasar, Bali, Indonesia
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Jurnal Veteriner
Published by Universitas Udayana
ISSN : 14118327     EISSN : 24775665     DOI : https://doi.org/10.19087/jveteriner
Core Subject : Health,
Veterinary
Jurnal Veteriner memuat naskah ilmiah dalam bidang kedokteran hewan. Naskah dapat berupa: hasil penelitian, artikel ulas balik (review), dan laporan kasus. Naskah harus asli (belum pernah dipublikasikan) dan ditulis menggunakan bahasa Indonesia atau bahasa Inggris. Naskah ilmiah yang telah diseminarkan dalam pertemuan ilmiah nasional dan internasional, hendaknya disertai dengan catatan kaki
Arjuna Subject : -
Articles 9 Documents
 1 
Search results for , issue "Vol 10 No 3 (2009)" : 9 Documents clear
Produksi dan Isolasi Protein Membran Stadium Bradizoit Toxoplasma gondii : Suatu Usaha untuk Mendapatkan Material Diagnostik dalam Mendiagnosa Toksoplasmo Muhammad Hanafiah; Wisnu Nurcahyo; Mufti Kamaruddin; Fadrial Karmil
Jurnal Veteriner Vol 10 No 3 (2009)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

A study was conducted to isolate the membrane protein of Toxoplasma gondii at bradyzoid stage forthe development of intradermal diagnostic test in livestock infected by the parasite. Toxoplasma wasinitially collected from meat of goat serologically positive to the parasite. The infected meat was then fedinto uninfected cat to obtain oocyst. The oocyst was inoculated into the stomach of mice to produce tachyzoitwhich in turn produce cyst in tissue known as bradyzoit . The membrane protein was then isolated from thebradyzoit. The protein was analysed by sodium dodecyl sulfate electrophoresis (SDS-PAGE). The dataobtained were presented descriptively. The protein concentration isolated from each mouse infected at thedose of 1x107 oocysts was 11.91 mg. Two protein bands specific for bradyzoit were identified at 97.72 kDaand 67.60 kDa.
Minyak Buah Merah Meningkatkan Aktivitas Proliferasi Limfosit Limpa Mencit Setelah Infeksi Listeria Monocytogenes Ika Wahyuniari; Marsetyawan HNE Soesatyo; Muhammad Ghufron; Yustina -; Andwi Ari Sumiwi; Sri Wiryawan
Jurnal Veteriner Vol 10 No 3 (2009)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

The success of individual in keeping healthy from infectious disease is closely related to the ability oftheir body immune responses againts infectious agents. It is reported that one way to increase the immuneresponses is by system to antioxidant. Red fruit oil contains carotenoid and tocoferol that function asantioxidant. This study was aimed at investigating the effect of red fruit oil on spleen lymphocyteproliferation in mice infected with Listeria monocytogenes. Sixty female Balb/c mice were used in thisstudy. The animals were randomly selected and divided into five groups, each group were 12 mice. The firstand second groups received aquadest only, whereas the third, fourth, and fifth groups received red fruit oilwith different doses, ie. 0.3, 0.6, and 1.2 mL/kbBW/day, respectively. Lymphocyte proliferation activitieswere tested by using 3-(4,5-dimethyl-thiazol-2-yl)-2,5-diphenyl-tetrazolium bromide (MTT) assay method.Lymphocyte proliferation assay demonstrated that there was significant difference between the groupswith red fruit oil and aquadest, started at day 2 post-infection. The optical density (OD) values recorded atday 3 in groups I, II, III, IV, and V were 0.769 ± 0.025, 0.904 ± 0.048, 1.110 ± 0.020, 1.021 ± 0.033, 0.979 ±0.002, respectively. The highest optical density (OD), ie. 1.194 ± 0.032, occurred at day 6 after receiving 0,3mL/kgBW/day. In conclusion, red fruit oil could increase lymphocyte proliferation.
Produksi IgY Antivirus Avian Influenza H5N1 dan Prospek Pemanfaatannya dalam Pengebalan Pasif I Wayan Teguh Wibawan; Sri Murtini; Retno Damajanti Soejoedono; I Gusti Ngurah Kade Mahardika
Jurnal Veteriner Vol 10 No 3 (2009)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Immunoglobulin Y (IgY) in yolk has been shown in several studies to prevent both bacterial and viralinfections. This research was conducted to find evidence that IgY specific against avian influenza virus(AIV) of H5N1 subtype can be produced in a large quantity in egg yolk. Laying hens were vaccinated withAI killed-vaccine (IPB-Shigeta). The IgY was purified using affinity chromatograpy technique, and anti-H5activity was measured using a standard haemaglutination inhibition (HI) and agar gel immunodifusion.The concentration of IgY was calculated, and the protein pattern was detected using polyacrilamid gel(AGID) electrophoresis (PAGE). Anti H5 antibody as high as 27 – 29 HI units was detected and produce aspecific line of precipitation in AGID. The concentration of IgY was 7.89 mg/ml. Purified specific IgY consistof 6 main protein bands with molecular weights ranging from 35 to 225 kD. These proteins were sensitiveto heat treatment (75oC for 30 minutes), to acid condition (pH2) as well as the pepsin and trypsin. Theseresults indicated the possibility of using specific IgY for passive immunisation to prevent AIV infection oras immunotherapeutic applications for AI treatment in humans.
Distribusi Gen Enterotoksin Staphylococcus aureus dari Susu Segar dan Pangan Asal Hewan Siti Isrina Oktavia Salasia; Khusnan -; Sugiyono -
Jurnal Veteriner Vol 10 No 3 (2009)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Staphylococcus aureus is a potential pathogen causing disease in human and animals due to severalvirulence factors. Staphylococcal enterotoxins which is responsible for foodborne disease is considered tobe one of the important virulence factor for the bacteria. The research was conducted to identify variousenterotoxin genes of S. aureus. Twenty three S. aureus isolates from milk cows (12 isolates) and foodanimal products (11 isolates) were used to study various enterotoxins genes (sea, seb, sec, sed, see, seg, seh,sei, and sej). The enterotoxins genes of S. aureus were investigated by using polymerase chain reaction(PCR) with specific primers. There were 3 isolates (13.04%) negative for staphylococcal enterotoxin genes.Twenty isolates (86.96%) harboured for one or more staphylococcal enterotoxin genes such as: sec (6 isolates/26.09%), see, seh for 1 isolate (4.35%), combination of 2 genes se (b,i), se (c,g), se (g,i) for 1 isolate of each(4.35%), se(c,e) for 2 isolates (8.70%), se(b,c) for 4 isolates (17.39%). Staphylococcal enterotoxin could bedetected in 3 combination genes of se(b,c,i), se(c,e,i), se(c,g,i) for 1 isolate of each (4.35%).
Respon Imun Itik Bali terhadap Berbagai Dosis Vaksin Avian Influenza H5N1 Ida Bagus Kade Suardana; Ni Made Ritha Krisna Dewi; I Gusti Ngurah Kade Mahardika
Jurnal Veteriner Vol 10 No 3 (2009)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

A study was carried out to investigate the immune response of Bali ducks against various doses ofAvian Influenza H5N1 vaccine. The study was carried out using a complete Random-Split in Time researchdesign as many as 40 of Bali ducks of 3 months age were kept separately in 4 groups. The ducks werevaccinated twice in two week interval with AI H5N1 vaccine of 0 (as negative control), 1/2, 1, and 2 doses.Sera were collected one day before first vaccination, then every week until three weeks after the secondvaccination. All sera were tested by hemaglutination inhibition (HI) test. The result shows that antibodylevel with double dose was significantly higher than single dose, half dose, and negative control (P<0.01).However antibody level in ducks vaccinated with single and half dose did not show any significant difference(P > 0.05).
Seleksi Spermatozoa Domba Garut dengan Metode Sentrifugasi Gradien Densitas Percoll Heri Sujoko; Mohamad Agus Setiadi; Arief Boediono
Jurnal Veteriner Vol 10 No 3 (2009)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Preparation of a good quality of sperm is required in order to increase the efficacy of in vitro fertilization.One method of a good sperm preparation is the use of Percoll density gradient centrifugation. This studywas aimed to determine the optimal combination of speed and time of centrifugation for the spermpreparation with the lowest mortality rate. Fresh semen collected from Garut sheep with the concentrationof 2 million cells per ml was used in this study. The experimental design used was a completely randomizedfactorial design with one control group and sixteen combination of speed and time of centrifugation. Theywere 1),(1) 200 xg for 5 min; (2) 200 xg for 10 min; (3) 200 xg for 15 min; (4) 200 xg for 20 min; (5) 300 xg for5 min; (6) 300 xg for 10 min; (7) 300 xg for 15 min; (8) 300 xg for 20 min; (9) 400 xg for 5 min; (10) 400 xg for10 min; (11) 400 xg for 15 min; (12) 400 xg for 20 min; (13) 500 xg for 5 min; (14) 500 xg for 10 min; (15) 500xg for 15 min; and (16) 500 xg for 20 min. All of control and treatments combination were replicated forthree times. Each control and combined treatment were replicated for three times. The variables observedwere the concentration of sperm, the percentage of live sperms, the percentage of progressively motilesperms and the percentage of normal sperm. The data obtained were analyzed by two way analysis ofvariance (ANOVA) and the differences between treatment groups were subjected for Duncan’s MultipleRange Test (DMRT). The best result was obtained using 400 xg for 15 minutes which showed the spermconcentration of 66.66 ± 12.22 millions per ml, the living sperms of 74.82± 1.53%, the percentage ofprogressively motile sperm of 57.56±8.42% and the percentage of normal sperm of 86±1.73%).
Analisis Faktor-Faktor Resiko Infeksi Cacing Pita pada Ayam Ras Petelur Komersial di Bogor Elok Budi Retnani; Fadjar Satrija; Upik Kesumawati Hadi; Singgih Harsoyo Sigit
Jurnal Veteriner Vol 10 No 3 (2009)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

A cross-sectional study was conducted in Bogor Region, West Java for two months from June to July2006. The aim of this research was to identify the risk factors of cestode infection in commercial cagedlayer chickens. A total of 202 chicken samples were collected from ten commercial caged layer chickenfarms. The risk factors assumption included host factors, farm environment and management characteristic.Logistic regression model showed that cestode infection risk association (P<0,01) to host age, (P<0,05) todry climate condition and open house farm management characteristic. This suggests that >50 monthshave higher risk (OR=5.6) than <20 months host age, dry climate condition have higher risk (OR=3.75)than wet, and open house farm management have higher risk (OR=27.24) than close house on the cestodesinfection.
Laktosa Mempertahankan Daya Hidup Spermatozoa Kambing Peranakan Etawah yang Dipreservasi dengan Plasma Semen Domba Priangan Demianus Ferdinand Souhoka; Michel Johan Matatula; Wilmientje Marlene Mesang-Nalley; Muhammad Rizal
Jurnal Veteriner Vol 10 No 3 (2009)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

The purpose of this research was to examine the effect of lactose in Tris extender on theviability of Etawah crossbreed goat spermatozoa which is preserved in the plasma using Prianganram seminal plasma at 3–5oC. Semen was collected using artificial vagina once a week. Freshsemen was divided into four tubes, and centrifuged at 3,000 RPM for 30 min. Supernatant wasremoved and replaced which an equal volume with Priangan ram seminal plasma. Semen in firsttube was diluted with Tris extender-20% egg yolk without lactose (control). Semen in second,third, and fourth tubes were diluted with Tris extender-20% egg yolk and 0.3% (0.3 g per 100 mlextender) (L0.3), 0.6% (L0.6), and 1.2% (L1.2) lactose, respectively. Diluted-semen were stored inrefrigerator at 3–5oC. Quality of diluted-semen including percentages of motile spermatozoa (MS),live spermatozoa (LS), and intact plasma membrane (IPM) were evaluated daily during storage at3–5oC for four days. The data were analyzed by usi analysis of variance (ANOVA). Means werecompared significant difference test at 0.05 significant level. Results of this study showed thatmean volume, colour, consistency, pH, mass activity, spermatozoa concentration, MS, LS,spermatozoa abnormal, and IPM of Etawah crossbreed goat fresh semen were 0.68 ml, cream,thick, 7, ++/+++, 4,148.57 million cell/ml, 70%, 83.89%, 7.12%, and 84%, respectively. Addition oflactose in Tris extender could maintain the quality of semen during preservation. At day-5 ofstorage, the percentages of MS, LS, and IPM for treatment L0.6 (40, 55.6, and 53.8%) weresignificantly (P<0.05) higher than treatment L1.2 (36, 50.2, and 51.8%), treatment L0.3 (34, 51.2,and 48.4%), and control (29, 41.4, and 41.8%). In conclusion, addition of 0.6% lactose in Trisextender is the best dose in maintaining the quality of Etawah crossbreed goat spermatozoa, andcould be maintaining percentage of motile spermatozoa 40% for four days after preserved at 3–5oC.
Analisis Faktor Risiko Penyakit Distemper pada Anjing di Denpasar I Gusti Made Krisna Erawan; I Nyoman Suartha; Emy Sapta Budiari; Diana Mustikawati; I Wayan Batan
Jurnal Veteriner Vol 10 No 3 (2009)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

A study was conducted to identify the risk factors of canine distemper in Denpasar, Bali. Risk factorsfor canine distemper were characterized using hospital records of private veterinary practitioners. Thisstudy showed that there was no difference in the susceptibility to canine distemper virus infection betweenmales and females. The occurrence of canine distemper disease is not significantly affected by the season.The risk of canine distemper disease for young dogs, between 0 and 1 year was 4.95-fold increase ascompared the risk of disease for dogs that were older than 1 year (Oods-Ratio: 4.95). Incomplete vaccinationwas associated with a 3.77-fold increase in the risk of canine distemper (Oods-Ratio: 3.77).

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