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I G. Made Krisna Erawan
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Jurnal Veteriner
Published by Universitas Udayana
ISSN : 14118327     EISSN : 24775665     DOI : https://doi.org/10.19087/jveteriner
Core Subject : Health,
Veterinary
Jurnal Veteriner memuat naskah ilmiah dalam bidang kedokteran hewan. Naskah dapat berupa: hasil penelitian, artikel ulas balik (review), dan laporan kasus. Naskah harus asli (belum pernah dipublikasikan) dan ditulis menggunakan bahasa Indonesia atau bahasa Inggris. Naskah ilmiah yang telah diseminarkan dalam pertemuan ilmiah nasional dan internasional, hendaknya disertai dengan catatan kaki
Arjuna Subject : -
Articles 12 Documents
 1   2 
Search results for , issue "Vol 12, No 3 (2011)" : 12 Documents clear
Kloning, Sikuensing dan Analisis Filogenetik Gen Nukleokapsid Protein Virus Tetelo Isolat Bali-1/07 Anak Agung Ayu Mirah Adi; I Made Kardena; Nyoman Mantik Astawa; Ketut Santhia Adhy Putra; Yoshihiro Hayashi; Yasunobu Matsumoto
Jurnal Veteriner Vol 12, No 3 (2011)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (343.619 KB)

Abstract

A study was carried out on the molecular characteristics of gene encoding for nucleocapsid protein(NP) of Newcastle disease virus (NDV) Bali-1/07. A portion of the fragment gene was amplified by reversetranscriptase-polymerase chain reaction (RT-PCR). The RT-PCR product purified from the gel was treatedwith Taq polymerase and cloned into plasmid pT7blueT vector. The recombinant plasmid was tranfectedinto Nova blue singles strain of Escherichia coli-competent cells and selected using white and blue colorselection method. Good white colonies were subcultured and tested for presence of expected gene fragmentby polymerase chain reaction (PCR). The correct size PCR product was recloned and sequenced. The PCRproduct of 540 base pairs were sequenced and aligned with several cognates genes of world NDVisolates.using Cluster IW. The phylogenetic analysis was then performed using MEGA 4. Phylogeneticanalysis showed that the NP gene of NDV Bali-1/07 is closely related with virulent NDVs such asGuangxii-11/03, KBNP-4152 and Ast2755/01 with nucleotide sequence homology of 92%, 91.4% and91%respectively, whereas the nucleotide sequence homology with avirulent NDVs such as LaSota and B1were 77%.
Profil Gonad Kodok Lembu Betina yang Diberi Human Chorionic Gonadotropin dan Ekstrak Hipofisis Kodok Lokal I Ketut Mudite Adnyane; Sevy Tiara Ilham; Muhammad Agil
Jurnal Veteriner Vol 12, No 3 (2011)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (249.868 KB)

Abstract

Bulllfrog (Rana catesbeiana) are naturally reach sexual maturity at the age of 3 years aftermetamorphosis. This research aims to study the gonad growth of female bullfrog. given human chorionicgonadotropin (hCG) and hipofise extract of local frog in order to accelerate the maturity. There were 40female bullfrog used in this study and divided into four treatment groups and one control. The treatmentswere injections of 150 IU hCG, 200 IU hCG, 8 hipofise, 16 extract of hipofise and controls. The injectionswere conducted once a month for four months. Every month after the first injection, two of bullfrogs fromeach group were measured their body and gonad weight, in addition, the stage of ovary was also observedeither in macroscopically and microscopically. The results showed that hCG treatment group gave betterresults compared with the treatment of hipofise extract of local frog. The injection of 200 IU hCG gave thebest result of gonad maturity index (IKG) 4.9%, compared with other treatment groups. Giving a dose of200 IU hCG and 16 hipofise exctract of local frog can be used to accelerate the maturity of female bullfrog

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