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Jurnal Veteriner
Published by Universitas Udayana
ISSN : 14118327     EISSN : 24775665     DOI : https://doi.org/10.19087/jveteriner
Core Subject : Health,
Veterinary
Jurnal Veteriner memuat naskah ilmiah dalam bidang kedokteran hewan. Naskah dapat berupa: hasil penelitian, artikel ulas balik (review), dan laporan kasus. Naskah harus asli (belum pernah dipublikasikan) dan ditulis menggunakan bahasa Indonesia atau bahasa Inggris. Naskah ilmiah yang telah diseminarkan dalam pertemuan ilmiah nasional dan internasional, hendaknya disertai dengan catatan kaki
Arjuna Subject : -
Articles 16 Documents
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Search results for , issue "Vol 13 No 3 (2012)" : 16 Documents clear
Aktivitas Antibakteri Ekstrak Jeruju (Acanthus ilicifolius) terhadap Pertumbuhan Vibrio harveyi Secara in vitro (ANTIBACTERIAL ACTIVITY OF JERUJU (ANACTHUS ILICIFOLIUS) EXTRACTS ON THE GROWTH OF VIBRIO HARVEYI IN VITRO) Gina Saptiani; Slamet Budi Prayitno; Sutrisno Anggoro
Jurnal Veteriner Vol 13 No 3 (2012)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

The aim of this research was to study the potential of jeruju (Acanthus ilicifolius) extract as anantibacterial for alternative therapy and control of bacterial diseases in prawn nurseries. Ethanol extractionwas prepared from jeruju’s leaves, trunks, fruits, and flowers..Each extract was prepared at differentconcentrations (50 ppm, 100 ppm, 200 ppm, 300 ppm, 400 ppm, 500 ppm, 600 ppm, 700 ppm, 800 ppm,900 ppm, and 1000 ppm, respectively) and further tested its antibacterial activity against Vibrio harveyiusing the agar disc diffusion method. The results showed that A. ilicifolius is a potential antibacterial,extract of the leaves seemed to be more effective in inhibit the growth of V. harveyi compared to other partsof the plant.
Sirkulasi Virus Flu Burung Subtipe H5 pada Unggas di Jawa Barat, Banten, dan Jawa Timur Sepanjang Tahun 2008-2009 (CIRCULATION OF AVIAN INFLUENZA OF H5 SUBTYPE ON BIRDS IN WEST JAVA, BANTEN AND EAST JAVA DURING 2008-2009) Dyah Ayu Hewajuli; Ni Luh Putu Indi Dharmayanti
Jurnal Veteriner Vol 13 No 3 (2012)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

The epidemic of avian influenza (AI) in Indonesia initially occurred at the end of 2003 which caused100% death of the affected chickens. It was caused by avian influenza virus (AIV) subtype H5. Recent datashowed that highly pathogenic avian influenza (HPAI)-H5N1 virus is still endemic among bird populationin Indonesia. A study was therefore conducted to find out the distribution of AIV-H5N1 in several regionsin Indonesia. Reverse transcriptase-polymerase chain reaction (RT-PCR) was used to detect the presenceof AI-H5 virus and hemagglutination inhibition (HI) test was used to detect the presence of anti-AIV-H5antibody. Results showed that anti-AIV-H5 antibody was detected in 36 % and was not detected in 64% oftested birds in West Java, Banten and East Java. The AIV-H5 antibody titer varied from low to high titer.The AIV-H5 was detected in samples from Cianjur (30%), Blitar (1.9&), Serang (12.5%) and pandeglang(17.5%). It was evident that AIV-H5 is still endemic in Indonesia.
Identifikasi Clinostomum complanatum Secara Molekuler pada Ikan Air Tawar di Yogyakarta dan Riau (IDENTIFICATION OF Clinostomum Complanatum FROM FRESHWATER FISH IN YOGYAKARTA AND RIAU BASED ON MOLECULAR STUDY) Morina Riauwaty; Kurniasih .; Joko Prastowo; Windarti .
Jurnal Veteriner Vol 13 No 3 (2012)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

The aim of study was, to identify Clinostomum complanatum (Digenea: Clinostomidae) infectingfreshwater fish in Yogyakarta and Riau on the bases of their molecular profiles in the internal transcribedspacer region (ITS1). Samples of climbing gouramy (Anabas testudineus) infected by Clinostomum sp. wereobtained from Kali Progo River, Yogyakarta. Whereas the climbing perch (Trichogaster trichopterus) wereobtained from the Sail River, Riau. Metacercariae of Clinostomum sp. found in the gills and visceralorgans were aseptically removed using needle, preserved in absolute ethanol. Molecular examination wasperformed by Polymerase Chain Reaction method consisted of extraction, amplification, electrophoresisand sequencing of DNA sample. The DNA sewuences of the samples were analysed by maximum parsimonyand neighbour-joining method. Phylogenetic analysis showed that Clinostomum sp. from Yogyakarta wasgenetically idential to Clinostomum complanatum, whereas Clinostomum sp. from Riau was geneticallysuspected as a new species (difference > 2%) which is included in one cluster to Clinostomum phalacrocorasis.
The Production and Use of Monoclonal Antibodies for the Detection of Avian Influenza Antigen in the in Infected Chickens Nyoman Mantik Astawa; Ida Bagus Kade Suardana; Gusti Ayu Yuniati Kencana
Jurnal Veteriner Vol 13 No 3 (2012)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

A safe and appropriate diagnostic method for avian influenza virus (AIV) infection in chickens wasestablished using monoclonal antibodies (mAbs) against the virus. The virus used for the production of themonoclonal antibodies was an Indonesian AIV-H5N1 isolate. Immortal mouse myeloma cells were fusedwith the lymphocytes derived from the spleen of mice immunized with the virus. The mAbs were tested fortheir specificity by enzyme linked immunosorbent assay (ELISA) and western blotting using formaldehydeinactivated virus and normal allantoic fluid as antigens. Twelve mAbs specific against AIV were isolatedand 8 mAbs were used for immunodetection of AIV antigen in chicken’s tissues. By indirect ELISA, themAbs were able to detect AIV antigen in allantoic fluid at the titre as low as 2-2 to 2-4 HA units per 0.1 ml.By immunoperoxidase staining AIV–antigen was detected in paraffin embedded tissues of AIV-infectedchickens. AIV antigen was not detected in chickens which were confirmed to be AIV negative. In theinfected chickens, high intensity of AIV antigen was detected in proventricle gland and small intestine.The AIV antigen with a lesser intensity was detected in lungs and spleen but hardly detected in muscle,brain and several other tissues. This study show clear evidences that mAbs produced in this study areapplicable for use in the detection of AIV antigen in infected chickens.
Aktivasi Oosit Menggunakan Strontium Klorida setelah Injeksi dengan Spermatozoa Domba Hasil Pengeringbekuan (OOCYTE ACTIVATION USING STRONTIUM CHLORIDE FOLLOWING INJECTION OF FREEZE-DRIED RAM SPERMATOZOA) Takdir Saili; Ita Djuwita; Mohamad Agus Setiadi; Srihadi Agungpriyono; Arief Boediono
Jurnal Veteriner Vol 13 No 3 (2012)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

One of the factors that inhibit the formation of male pronuclei following injection of freeze-dried ramspermatozoa was the absence of artificial activation during oocyte incubation after the injection. Therefore,in this experiment the ability of strontium chloride (SrCl2) to improve oocyte activation followingintracytoplasmic sperm injection (ICSI) was evaluated. Aceto lacmoid staining was used to assessdecondensation and pronucleus formation following ICSI. Results of this experiment revealed that freezedriedspermatozoa had the ability to decondense and to form 1PN following injection into oocytes evenwithout artificial activation, but failed to form 2PN. However, 40% of 2PN oocytes were obtained when theinjected oocytes was first incubated for 20 minutes in medium containing 50 mM strontium chloride thensubsequently incubated for 10 hours in medium without strontium. On the contrary, the 2PN oocytes werenot observed either in injected oocyte neither without artificial activation nor in non-injected oocytes withartificial activation. In conclusion, freeze-dried ram spermatozoa were able to decondense and to support2PN formation following ICSI and artificial activation using strontium.
Histologi dan Histomorfometri Testis dan Epididimis Muncak (Muntiacus muntjak muntjak) pada Periode Ranggah Keras (HISTOLOGY AND HISTOMORPHOMETRY OF THE TESTIS AND EPIDIDYMIS OF MUNTJAC (MUNTIACUS MUNTJAK MUNTJAK) DURING HARD ANTLER PERIOD) Sri Wahyuni; Srihadi Agungpriyono; Muhammad Agil; Tuty Laswardi Yusuf
Jurnal Veteriner Vol 13 No 3 (2012)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

The objective of this study was to describe the histology and histomorphometry of testis and epididymisof muntjac (Muntiacus muntjak muntjak) during hard antler period. The tissues of the testis and epididymisof an adult male muntjac were processed for histological examination and stained with haematoxylineosine(HE). The parenchyma of muntjac’s testis during hard antler period showed tubuli seminiferi waslined with germinal epithelium: spermatogonia, spermatocyte, spermatid that differentiated intospermatozoa. Sertoli cells were found among the germinal cells. In addition, Leydig cells were foundaround the blood vessel of interstitial tissue along with macrophages. Diameter of the seminiferous tubuleand epithelial thickness were 176,60±7,06 ?m and 50,27±3,62 ?m respectively. The epididymal duct wassubdivided into three segments: caput, corpus and cauda. They were lined predominantly withpseudostratified columnar epithelium which was varied in its thickness. The largest diameter of epididymalduct was found in cauda region (324,26±25,79 ?m), while caput epididymidis had the thickest of epithelialcell (62,21±4,21 ?m) and tended to ce thinner in corpus (49,53±3,01 ?m) and cauda epididymidis(16,30±2,27?m). The density of spermatozoa was observed the most in the lumen of cauda region comparedto caput and corpus epididymidis. In conclusion, the structure of histology and histomorphometry of theseminiferous tubule of testis and epididymal duct of muntjac were similar with small ruminants andother Cervidae during hard antler period.
Keterkaitan Panhisterektomi dan Suplemen 1,25- Dihidroksivitamin D3 dengan Risiko Urolitiasis pada Tikus (CORRELATION BETWEEN PANHISTERCTOMY AND 1.25-DIHYDROXYVITAMIN D3 SUPPLEMENTATION ON RATS UROLITHIASIS RISK) Hartiningsih .; Devita Anggraeni; Irkham Widiyono; Hastari Wuryastuti
Jurnal Veteriner Vol 13 No 3 (2012)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

The objective of this research was to study the correlation of panhisterectomy and supplement 1.25-dihydroxyvitamin D3 on urolithiasis risk in Wistar rats. Twenty female Wistar rats at 8 weeks of age, weredivided into four groups (control fed standard diet, control fed standard diet+1,25-dihydroxyvitamin D3 supplement, panhisterectomy fed standard diet and panhisterectomy fed standard diet +1,25-dihydroxyvitamin D3 supplement). Eleven weeks after treatment, each of rats was placed into individualmetabolic cage for balance study for a week. From day 4 to 11 of the balance study, every morning theremaining food, feces, and urine were collected and recorded for calcium (Ca) analysis. At the end ofbalance study, blood samples were taken from canthus retroorbitalis medialis for estrogen analysis. Theresults showed urinary and fecal Ca excretions were not significantly different compared to the controlgroup. Calcium consumption was significantly higher (P<0.05) in panhisterectomized rats compared withthose in control rats. While, estrogen in panhisterectomized group was not significantly different to thosein control rats. Calcium urinary and Ca consumption in rats consuming 1,25-dihydroxyvitamin D3 supplement were significantly higher (P<0.05) compared with those in without 1,25-dihydroxyvitamin D3 supplementation, but Ca excretion in feses was not significantly different. Estrogen in rats consuming1.25-dihydroxyvitamin D3 supplement was significantly lower (P<0.05) compared with the rats that without1,25-dihydroxyvitamin D3 supplemention. It can be concluded that panhisterectomy does not seem to affecturolithiasis risk, while 1,25-dihydroxyvitamin D3 supplement may affect urolithiasis risk. There is likelyno association between panhisterectomy and 1.25-dihydroxyvitamin D3 supplementation on urolithiasisrisk in Wistar rats.
Aktivitas Penyembuhan Luka oleh Gel Fraksi Etil Asetat Rimpang Kunyit pada Mencit Hiperglikemik (WOUND HEALING ACTIVITY OF AETHYL ACETATE OF CURCUMA LONGA GEL IN HYPERGLYCEMIC MICE) Ietje Wientarsih; Wiwin Winarsih; Lina Noviyanti Sutardi
Jurnal Veteriner Vol 13 No 3 (2012)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Traditional remedies generally use plant based therapies for treatments. The availability of plantfor treatments is relatively abundant in Indonesia, whether as treatments for diabetic wounds or antiinflammation. Curcuma longa Linn has been reported as an alternative treatment for several diseasesincluding wound healing. The aim of this study was to investigate the possible effect on wound healing ofethyl acetate of C.longa gel in skin hyperglycemic mice. The ethyl acetate of C.longa gel was evaluated toassess its healing efficiency on excision wound. Thirty mice were used in this study. The mice were dividedinto three groups i.e.: KN as a negative control (without treatment), KP as a positive control (Neomycinsulfate), and treated groups (GE= ethyl acetate gel). There was a significant effect on histopathologicalcharacteristics in wound healing of treated mice with ethyl acetate gel compared with KN mice. It seemthat C.longa gel is a potential for phyto-therapeutic agent in management of wound healing.
Ekstrak Sambiloto (Andrographis paniculata) Menurunkan Jumlah Skizon, Mikrogamet, Makrogamet, dan Oosista Eimeria tenella (EXTRACT OF ANDROGRAPHIS PANICULATA DECREASED SCHIZONTS, MICROGAMETES, MACROGAMETES AND OOCYSTS NUMBER OF EIMERIA TENELLA) UMI CAHYANINGSIH; RESSY RIANDCI; DYAH ISWANTINI
Jurnal Veteriner Vol 13 No 3 (2012)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

The aim of this research was to observe the effect of ethanol extract of Andrographis paniculata givenin grading doses to the schizonts, microgamete, macrogamete, and oocytes counts of Eimeria tenella inchicken caecum. A total of ninety day old broiler chicks were used in the study. At two weeks old the broilerswere divided into six groups. Each group consisted of 15 broilers, the 6 groups were: (i) negative control(broilers did not receive any treatment); (ii) positive control (each animal were infected with 104 E. tenellaoocytes); (iii) medicine control (each animal were infected with 104 E. tenella oocytes and coccidiostat); (iv)A1 (each animal were infected with 104 E. tenella oocytes and paniculata extract 90 mg/kg body weight); (v)A2 (each animal were infected with 104 E. tenella oocytes and paniculata extract 180 mg/kg body weight);and (vi) A3 (each animal were infected with 104 E. tenella oocytes and paniculata extract 360 mg/kg bodyweight). At day 6, 9, 13, 16, and 22 post infection three broilers from each group were sacrificed and theirceca were collected for histopathological examination. The results showed that paniculata extract at dose90 mg/kg body weight and 180 mg/kg body weight was able to decrease the numbers of shizont, microgamete,macrogamete, and oocytes of E. tenella in the chicken caecum.
Perbandingan Angka Fertilitas dan Hambatan Perkembangan Embrio Mencit yang Dikultur dalam Medium M16 dan Human Tubal Fluid (THE COMPARISON OF MICE FERTILITY RATE AND EMBRYONIC DEVELOPMENT CELL BLOCK WHEN CULTURED IN M16 AND HUMAN TUBAL FLUID MEDIA) Widjiati .; Sri Endah Pusporini; M. Zainal Arifin
Jurnal Veteriner Vol 13 No 3 (2012)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

The aim of this research was to compare the fertility rate and embryonic development cell block ofmice when cultured in M16 and Human Tubal Fluid (HTF) media, respectively. Two months old femaleBalbC mice were super ovulated using Pregnant Mare Serum Gonadotrophin (PMSG) and Human ChorionicGonadotrophin (HCG) prior to mating with vasectomies mice. At 17 hours post mating the mice wassacrificed for the collections of egg cells and spermatozoa. Egg cells were collected by tearing the fertilizationsac, while the sperm were collected from caudal epididymis. After the collection, both the egg cells andsperm were put in Petri dish containing M16 and HTF media and kept in 5% CO2 incubator at 370C for onehour prior to the in vitro fertilization (IVF) was performed. In vitro fertilization was performed in 5% CO2 incubator at 370C and kept for 24 hours in M16 and in HTF culture media. The results showed thatfertilization rate was 98.09% and 99.57%; cell block embryonic development was 85.09% and 83.36%when cultured in M16 and HTF media, respectively. In conclusion, HTF media can be used for culturingmouse embryo.

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