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Jurnal Veteriner
Published by Universitas Udayana
ISSN : 14118327     EISSN : 24775665     DOI : https://doi.org/10.19087/jveteriner
Core Subject : Health,
Veterinary
Jurnal Veteriner memuat naskah ilmiah dalam bidang kedokteran hewan. Naskah dapat berupa: hasil penelitian, artikel ulas balik (review), dan laporan kasus. Naskah harus asli (belum pernah dipublikasikan) dan ditulis menggunakan bahasa Indonesia atau bahasa Inggris. Naskah ilmiah yang telah diseminarkan dalam pertemuan ilmiah nasional dan internasional, hendaknya disertai dengan catatan kaki
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Articles 17 Documents
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Search results for , issue "Vol 15 No 2 (2014)" : 17 Documents clear
Efektivitas Air Buah Lontar dalam Mempertahankan Motilitas, Viabilitas dan Daya Tahan Hidup Spermatozoa Sapi Bali Thomas MataHine; Burhanuddin .; Aloysius Marawali
Jurnal Veteriner Vol 15 No 2 (2014)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

The objective of this study was to determine the effectiveness of palmyra juice in maintaining motility,viability, and longevity of bali cattle sperm during in vitro storage. Semen collected from a four year oldbali bull had been trained to serve an artificial vagina. Good quality semen divided into four tubes to betreated with: without extender (negative control), citrate-egg yolk (citrate-EY) and coconut water-egg yolk(CW-EY) as positive control; and palmyra juice-egg yolk (PJ-EY), and then stored in a refrigerator attemperature 3 - 8oC. Each treatment was repeated five times to form 20 experimental units. The resultsshowed that up to the fourth day of storage, sperm preserved in PJ-EY had motility 44%, higher (P<0.05)than citrate-EY, CW-EY, and without extender, i.e 30%, 18%, and 0%, respectively. PJ-EY extender alsoyield sperm viability 60%, higher (P<0.05) than citrate-EY, CW-EY, and without extender i.e 50%, 32% and0%, respectively. Sperm livability achieve 11.20 days in PJ-EY, longer (P <0.05) than citrate-EY, CW-EYand without extender, i.e 9.60 days, 7.60 days , and 1.31 days, respectively. In conclusion that palmyrajuice-egg yolk extender effective to maintain motility, viability, and longevity of bali cattle sperm duringin vitro preservation.
Keberadaan Bakteri Listeria monocytogenes pada Keju Gouda Produksi Lokal dan Impor (PRESENCE OF LISTERIA MONOCYTOGENES IN LOCAL AND IMPORTED GOUDA CHEESES) Debby Fadhilah Pazra; Trioso Purnawarman; Denny Widaya Lukman
Jurnal Veteriner Vol 15 No 2 (2014)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Listeria monocytogenes is included in the foodborne pathogen, which has been associated with severaloutbreaks of human listeriosis especially in high risk groups. Listeria monocytogenes could be found inGouda cheeses because of poor hygienic and sanitation practices. In addition, this bacteria could surviveduring the making of cheese and cheese ripening process. The purpose of this study was to identify thepresence of L. monocytogenes in local and imported Gouda cheeses and how the safety level of the Goudacheese against contamination of L. monocytogenes. This study used the conventional method in accordancewith the Bacteriological Analytical Manual, US Food and Drug Administration and Bergey’s Manual ofDeterminative Bacteriology to detect the presence of L. monocytogenes at 15 samples of local Gouda cheeseand 15 samples of imported Gouda cheese sold in supermarkets in Jakarta and Bogor. The results of thisstudy showed that was not found L. monocytogenes in local and imported Gouda cheese. It could be concludedthat is Gouda cheese relatively safe from L. monocytogenes and meets Indonesian National Standard.
Immunological Detection of Newcastle Disease Viral Antigen in the Naturally Infected Chickens by Monoclonal Antibodies against Fusion-2 Protein Nyoman Mantik Astawa; Anak Agung Ayu Mirah Adi
Jurnal Veteriner Vol 15 No 2 (2014)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Monoclonal antibodies (mAbs) against Fusion (F)2 protein of Newcastle disease virus (NDV) wereproduced for the detection of the viral antigen in infected chickens. Cells derived from spleen of Balb/c miceimmunized with the virus were fused with mouse myeloma cells to generate hybridomas capable ofproducing mAbs against the virus. The hybridomas were screened by enzyme-linked immunosorbent assay(ELISA) for anti-NDV specific mAbs using crude viral antigen (allantoic fluid of NDV-infected fertile eggs)and normal uninfected allantoic fluid of fertile eggs as negative control. The NDV proteins reactive withmAbs were then determined by Western Blotting using purified NDV as antigen. The mAbs reactive withF2 (12.5 KDa) protein of NDV were then used for the detection of NDV antigen in both the allantoic fluidof NDV- infected chicken embryos and in organs of naturally infected chickens. The results showed that 2out of 5 mAbs produced were against F2 protein of NDV. By indirect ELISA, the mAbs were able to detectthe viral antigen in allantoic fluid of NDV infected fertile chicken eggs at the titre as low as 2-2 to 2-4 HAunits per 0.1 mL. NDV–antigen was also detected by immunoperoxidase staining in paraffin-embeddedtissues of NDV-infected chickens but not in normal uninfected chickens. The most prominent infection wasdetected in the gastrointestinal tract and the lung. The NDV antigen was also detected in other organssuch as the brain, spleen, and several other tissues. It is evident that mAbs produced against F2 proteinof NDV were applicable for use in the detection of NDV antigen in infected chickens.
Pencegahan Gangguan Fungsi Ginjal Karena Stres Oksidatif pada Tikus Diabetes dengan Ubi Jalar Ungu I Made Jawi; I Wayan Sumardika; Ni Made Linawati
Jurnal Veteriner Vol 15 No 2 (2014)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Recently, anthocyanins-rich food, is believed to prevent tissue damages due to oxidative stress. This studyaimed to investigate the protective effect of water extract of Balinese purple sweet potatoes which containhigh anthocyanins to the renal tissues of diabetic rats. A total of 30 adult male rats were divided into threegroups using randomized post-test only control group design, respectivelly. Animals in group 1 (diabeticgroup) were given streptozotocin 60 mg/kg; group 2 (diabetic+extract/ treatment group) at 3 days after theanimals were given streptozotocin they were given 4 mL per day of the extract for 50, group 3 (control group)animals were given placebo. At day 50 of the treatment, the blood and renal tissue superoxidase dismutase(SOD) and malondialdehyde (MDA), creatinin and blood urea nitrogen (BUN) level of all rats were measured.The data were analyzed using analysis of variance. The results showed a significant increase of blood andrenal tissues MDA and BUN level in group 1 (p<0,05). In addition, a decrease of SOD and creatinin level(p<0,05) were also seen in this group. In comparison to animals in group 2, the increase of blood MDA andBUN level as well the decrease of SOD and, creatinin level were significantly lower (p<0,05). From thesefindings, it can be concluded that aqueous extract of Balinese purple sweet potato can prevent renalfunction disorders due to oxidative stress.
Pembelian Ternak dan Kelembaban Tinggi Merupakan Faktor Risiko Leptospirosis pada Sapi di Girimulyo, Kulon Progo, Jogjakarta (PURCHASE OF CATTLE AND HIGH HUMIDITY WERE RISK FACTORS OF BOVINE LEPTOSPIROSIS IN GIRIMULYO, KULON PROGO DISTRICT) Guntari Titik Mulyani; Bambang Sumiarto; Yuriati .
Jurnal Veteriner Vol 15 No 2 (2014)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Leptospirosis is an acute infectious disease could to attack humans and animals such as dogs, cats,cows, pigs, and wild animals such as rats, weasels and squirrels. The death rate of human leptospirosis inIndonesia reached 2.5 to 16.5%. Leptospirosis attacked approximately 274 residents, 18 fatalities atKulonprogo disctrict, and designated as Extraordinary Events in 2011. The purpose of this reasearch is todetermine risk factors affecting the prevalence of leptospirosis in cattle in the area of human leptospirosisin Girimulyo, Kulon Progo. This study used 132 samples of cattle blood. Methods of examination withMicro Aglutination Test (MAT) conducted at the Center for Veterinary Research, Bogor. Micro AglutinationTest is done by creating a serum dilution with PBS in stages then added antigen in the form of various cultures leptospira serovar. Subsequently incubated at 28-30 ° C for 2 hours, and was reading the resultsunder a microscope dark field / phase contrast. Endpoint reading was 50% agglutination, highest finalserum dilution in serum-antigen mixtures that showed 50% agglutination is called the titer. Serum witha titer of 1:100 or more against one or more tested positive for serovar. Micro Aglutination Test result wasthe dependent variable (Y), while the independent variables (X) were factors of farmers, animal factors,enclosure factors, and feed factors. Univariate analysis was done to see descriptively each variable studied.Association between the prevalence of leptospirosis and risk factors was analyzed by Chi Square.Multivariate Logistic Regression Analysis was used to build models of leptospirosis disease to investigatethe risk factors associated with leptospirosis. Variables that influence the prevalence of leptospirosiswere measured magnitude of association by Odd Ratio. The results of study that the prevalence of bovineleptospirosis in Girimulyo subdistrict, Kulonprogo district was 7.6%. Risk factors for the prevalence ofleptospirosis in cattle in Girimulyo subdistrict, Kulonprogo district were livestock purchased factor (OR5.49) and a high humidity factor (OR 1.65). These results can be concluded that leptospirosis infection incattle is high (7.6%) in the area of human leptospirosis. The possibility cattle as a source of infection inhumans still needs further research.
Perbandingan Pengencer Andromed dan CEP-2 terhadap Kualitas Spermatozoa Sapi Hasil Seksing dengan Sentrifugasi Gradien Densitas Percoll (COMPARISON OF ANDROMED AND CEP-2 DILUTER ON THE QUALITY OF LIMOUSIN CATTLE SPERMATOZOA FOLLOWING SEXING WITH PERCOLL G Juniandri .; Trinil Susilawati; Nurul Isnaini
Jurnal Veteriner Vol 15 No 2 (2014)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

The use of appropriate diluent is intended to ensure the physical and chemical requirements ofspermatozoa so that the quality, particularly capacitation can be maintained. This study aimed todetermine the quality of Limousin cattle sperm after sexing with density percoll centrifugation methodusing Andromed diluent and Cauda epididymal Plasma 2 (CEP-2) plus 10% egg yolk. Observations ofspermatozoa quality including : motility, viability, percentage of abnormal, concentrations, membraneintegrity and state of capacitation. The semen used were obtained from Limousin cattle at the Center forArtificial Insemination(BBIB) Singosari Malang, with motility criteria e” 70%. Data were analysed usinga paired T-test in order to determine differences in the use of thinning treatments at each layer. Theresults showed that both diluent, andromed and CEP-2 diluent plus 10% egg yolk were able to maintainthe quality of spermatozoa either in the upper and lower layers
Sidik Jari DNA dan Fenotipe pada Populasi Kambing Gembrong dengan Status Kritis di Karangasem, Bali (DNA FINGERPRINT AND PHENOTYPE ON THE CRITICAL POPULATION STATUS OF GEMBRONG GOAT IN KARANGASEM REGENCY, BALI) Moch Syamsul Arifin Zein; Sri Sulandari
Jurnal Veteriner Vol 15 No 2 (2014)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Gembrong goat is animal genetic resources which endemic in Bali island and only exists in Karangasemregency. The current population status of the gembrong goat is critical. Therefore, the population developmentof in-situ or ex-situ needs to be conducted. Close relative mating tend to occur in conditions such as in asmall population, critical status or limited distribution. Low degree of genetic variability is often found insuch conditions. Mating arrangements based on DNA data of fingerprint was developed in this study toavoid mating between close relatives (inbreeding). Pedigree detection was analyzed using fifteen (15)microsatellite markers as recommended by ISAP/FAO. The results showed that polymorphic alleles foundin the microsatellite markers of SRCRSP3, ILSTS005, MCM527 (4 alleles), followed BM1818 (3 alleles),and ILSTS029, BMS1494, MAF035, OARFCB20, OARE54, MAF70, ILSTS11, ETH10 (2 alleles).Monomorphic allele (1 allele) was also found in microsatellite markers of SPS113, CSRD247, INRA0132.DNA fingerprint of the gembrong goat population was created by genetic distance between individuals,and indicated six clades / haplogroups. It is suggested from this study that mating arrangements betweendifferent clades should be applied to increase genetic diversity. Description of morphologies such as haircolor and body size, were used as basic considerations in determining the authenticity of gembrong goat.Among the population of gembrong goat in this study, hair color of the goat showed white (78.95%), amixture of light brown and white (15.79%), and a mixture of brown and black (5.26%). Gembrong goat bodysize in this study was still in the range of the previous studies. The analysis results of DNA fingerprintsand phenotypes can be used as a basis for rescue and development of gembrong goats, in an attempt toform a large gene pool with high viability for the conservation, development and sustainable uses.
Pemberian Duddingtonia flagrans dan Saccharomyces cerevisiae Meningkatkan Produksi Susu dan Menurunkan Populasi Cacing pada Sapi (THE TREATMENT OF DUDDINGTONIA FLAGRANS AND SACCHAROMYCES CEREVISIAE INCREASE MILK PRODUCTION AND DECREASE WORM POPULATION IN Riza Zainuddin Ahmad; Djaenudin Gholib
Jurnal Veteriner Vol 15 No 2 (2014)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Duddingtonia flagrans isolates are commonly used as biological control, and Saccharomyces cerevisiaeas probiotics. This study aimed to test isolates D. flagrans and S. cerevisiae as a biological agent andprobiotics. Tests carried out on beef cattle and dairy cows, which grouped into treatment (Administrationof D. flagrans and S. cerevisiae) and controls. For examination worm was also made in the grass. The studywas done in seven months The results of this study indicate that there is increase of 10 to 15% of the milkproduction in dairy cows after treatment (p> 0.05) but the beef was no difference between the treatmentand control. Treatment of D. flagrans significantly reduced the population of worms in the grass andanimals. Base on the result, it was concluded that D. flagrans and S. cerevisiae can be used together toincrease milk production and reduce the population of worms in cattle.
Kejadian Dermatosis yang Tinggi pada Anjing Jalanan di Bali (A HAIGH DERMATOSIS INCIDENCE AMONG STRAY DOGS IN BALI) I Kadek Saka Wiryana; I Made Damriyasa; Nyoman Sadra Dharmawan; Kadek Agus Agra Arnawa; Kadek Dianiyanti; Dina Harumna
Jurnal Veteriner Vol 15 No 2 (2014)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

This study was designed to investigate the incidence of dermatosis among stray dogs in Bali. A totalof 401 stray dogs collected in the animal shelter of Bali Animal Welfare Association during 2011 wasinvestigated in this study. Dogs were examined by the clinical sign and continued by skin scraping, cytologyand wood lamp examination. In total, 37,9% sample were positive for dermatosis. Bacterial causeddermatosis found to be the most prevalent (23,6%), followed by tick and fleas (16.5%), scabies (12.7%),malassezia (8.2%), demodek (8%) and ringworm (4.5%) respectively. We also found that dermatosis weremore prevalent in male dogs (50.9%) rather than female dogs (32.9%). Dogs 9-12 weeks old were moreheavily infected (45.8%) than other group. In conclusion, the incidence of dermatosis among stray dogs inBali is relatively high. This may need serious awareness as some of this dermatosis were zoonotic tohuman.
Gambaran Fungsi Jantung Kelinci Domestik Secara Ekhokardiogram pada Anestesi Propofol dan Isoflurane Jangka Panjang (ECHOCARDIOGRAM PROFILE OF CARDIAC FUNCTION IN DOMESTIC RABBITS DURING LONG-TERM ANESTHESIA PROPOFOL AND ISOFLURANE) Sitaria Siallagan; Gunanti .; Deni Noviana
Jurnal Veteriner Vol 15 No 2 (2014)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Several major surgeries may require long time use of anesthetics and therefore increase the heartfunction suppression. The purpose of this study was to determine long term effects of anesthetic propofoland isoflurane on heart structure and function of domestic rabbits through observation of the blood flowvolume and contraction capabilities by M-mode echocardiography examination. This study used thecombination of propofol 12.5 mg/kg IV as an induction and isoflurane inhalation as the maintenanceanesthetic for a 12-hour observation. Parameters observed in this study were heart rate, wall thickness,internal chamber dimension, blood flow volume, fractional shortening, and ejection fraction. Results showedthat the left ventricle heart chamber dimensions during diastole (LVIDd) and systole (LVIDs) were between1.21±0.08 to 1.33±0.1 cm, and 0.87±0.08 to 0.98±0.08 cm respectively. Left ventricular wall thickness atdiastole (LVWd) and systole (LVWs) ranged from 0.24±0.04 to 0.28±0.04 cm, and 0.23±0.03 to 0.28±0.05cm respectively, while the thickness of the interventricular septa at diastole (IVSd) and systole (IVSs)ranged from 0.23±0.02 to 0.27±0.04 cm, and 0.28±0.06 to 0.30±0.05 cm. Heart rate ranged from 244.6±18.46to 266.4±24.55 beat/min. Heart flow volumes which included stroke volume (SV) and cardiac output (CO)ranged from 1.07±0.29 to 1.57±0.53 mL/beat and 0.27±0.07 to 0.39±0.14 L/min respectively. Fractionalshortening and ejection fraction ranged from 26±3 to 31±5% and 60±2 to 67±8% respectively. In conclusion,long term anesthesic of propofol and isoflurane combination induced minimum cardiac suppression indomestic rabbits.

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