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Jurnal Veteriner
Published by Universitas Udayana
ISSN : 14118327     EISSN : 24775665     DOI : https://doi.org/10.19087/jveteriner
Core Subject : Health,
Veterinary
Jurnal Veteriner memuat naskah ilmiah dalam bidang kedokteran hewan. Naskah dapat berupa: hasil penelitian, artikel ulas balik (review), dan laporan kasus. Naskah harus asli (belum pernah dipublikasikan) dan ditulis menggunakan bahasa Indonesia atau bahasa Inggris. Naskah ilmiah yang telah diseminarkan dalam pertemuan ilmiah nasional dan internasional, hendaknya disertai dengan catatan kaki
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Articles 23 Documents
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Search results for , issue "Vol 17 No 4 (2016)" : 23 Documents clear
Isolasi dan Identifikasi Spesies Bakteri Asam Laktat Penghasil Senyawa Antimikrob Asal Kolon Sapi Bali (ISOLATION AND IDENTIFICATION OF LACTIC ACID BACTERIA SPECIES PRODUCING ANTI-MICROBIAL SUBSTANCE ISOLATED FROM COLON OF BALI CATTLE) Sri Anggreni Lindawati; I Wayan Suardana
Jurnal Veteriner Vol 17 No 4 (2016)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Bali cattle as one of the local cattle are known have uniquely genetic characteristics. They are easy toadapt at a less favorable environment, so that they are known as a pioneer cattle. According to theiruniquely, it may allow for the discovery of specific types of acid lactic bacteria compared with others. Thishypothesis is based on the assumption that the types of bacteria as a normal flora in the intestine tract ofcattle are highly dependent on several factors, and one of which is a feed factor. Based on the abovebackground, this study was conducted. The aim of study was to isolate and identify of a specific species oflactic acid bacteria that has anti-microbial substances. The study began by isolation of acid lacticbacteria originated from 20 fecal samples of colon of bali cattle through the growth on selective medium i.e.deMann, Rogosa, Sharpe (MRS) medium followed by Gram staining and catalase test. The screening ofantimicrobial activity of each isolate was performed by culturing of isolates again indicator bacterial on blood agar medium. The selected isolates were continuously tested on medium contains 15% NaCl ,medium with ph 9.6, and medium with temperature 10°C, respectively in order to identification genus ofbacteria. The final stage of identification in order to know the specific isolate, which has antimicrobialsubstances, was confirmated by using the API 50 CHL kit. The results of study showed that one of theisolates that known have widely antimicrobial activities was isolate with 3A code. This isolate hasinhibitory zone to indicator bacterial i.e. Staphylococcus aureus ATCC 29213 and Escherichia coli ATCC25922. This isolate is known as Lactococcus lactis ssp lactis 1 with its similarity value 65.7%. This isolateis potentially to continuously study in order to know the potency of isolate as a probiotic candidate and oras a food preservative.
Karakterisasi Morfometrik dan Jarak Genetik Rumpun-Rumpun Kelinci di Jawa Barat (MORPHOMETRIC CHARACTERIZATION AND GENETIC DISTANCE OF RABBIT BREEDS IN WEST JAVA) Rudi Dedi Iskandar; Bram Brahmantiyo; Rudi Priyanto
Jurnal Veteriner Vol 17 No 4 (2016)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

The objectives of this study were to assess morphometric characteristics, breeds relationship andvariables that distinguished among breeds of rabbits raised in West Java. This research used 419 rabbitsconsisted of Angora (AG), Dutch (DT), Flemish Giant (FG), Lop (LP), Netherland Dwarf (ND), Composite(PX), Rex (RX), Satin (ST), Reza (XA) and New Zealand White (ZW). Head length (PK), head width (LK), earlength (PTL), ear width (LTL), chest width (LD), chest depth (DD), chest circumference (LKD), body length(PB ), hips width (LP), length of the scapula bone (PS), humerus length (PH), radius-ulna length (PRU),femur length (PF) and tibia length (PT) were observed. Data were analyzed with analysis of variance,discriminant and canonical analysis using SAS program ver. 9.1.3 and MEGA5 program to get theconstruction of phenogram tree. FG and ST rabbits were generally larger in size and shape than the otherrabbits breeds, while ND rabbit had the smallest morphological size than other rabbits breeds, except forLK, LD and DD. Results of discriminant analysis showed that LP, RX, ND and XA had a high similarityvalue, otherwise DT, FG, ST, PX, AG and ZW had no the value. The closest genetic distance matrix valueindicated by PX-ZW breeds (1,53) and the farthest genetic distance indicated by FG-ND breeds (6,62).Phenogram tree construction showed that the breeds rabbits divided into five clusters, namely cluster ND,DT; ST clusters; FG cluster; cluster LP, PX, ZW and cluster AG, XA, RX. Phenotypic size that had stronginfluence on the differentiation of rabbit breeds were PTL, LTL, PRU, PH and PF on the canonical 1 alsoPT and PS on canonical 2.
Suplementasi Probiotik dan Temulawak pada Ayam Pedaging terhadap Populasi Salmonella sp dan kolesterol darah (PROBIOTICS AND TEMULAWAK SUPPLEMENTATION ON BROILER CHICKENS AGAINST SALMONELLA SP POPULATION AND BLOOD CHOLESTEROL LEVEL) Kartiawati Alipin; Ratu Safitri; Ruhyat Kartasudjana
Jurnal Veteriner Vol 17 No 4 (2016)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Probiotics is a live microbial feed supplements that improve the microbial balance in the digestivetract of cattle host. Temulawak (Curcuma xanthorrhiza Roxb) is one of native Indonesian spices that areuseful for prevention and treatment of human disease but also can be used for broiler chickens supplement.Bioactive contents known acting as an anti-bacterial, anti-inflammatory, and anti-fungal. The study wasconducted to determine the effects of probiotic and temulawak supplementation on population of Salmonellasp and broiler chickens blood cholesterol. This study used 300 DOC final stock. Probiotic concentration 109cell/mL administered through drinking water every day while of temulawak extract dose 0.25 g/L 0.50 g/Lonce a week, for five weeks. The variables measured were Salmonella sp populations and blood cholesterollevels. The results showed that combination administered of probiotics with temulawak dose 0.50 g/Ldecreased of population of Salmonella amount to 87.04% and decreased of blood cholesterol amount21,48% at administered of probiotics with temulawak dose 0,25 g/L. The conclusions of this study showedthat the percentage decrease of Salmonella sp population and blood cholesterol levels in combinationprobiotic and temulawak supplementation so that broiler produced is safe for consumption.
Cemaran Timah Hitam dalam Darah Sapi Bali yang Dipelihara di Tempat Pembuangan Akhir Kota Denpasar (BLOOD LEAD CONTAMINATION IN BALI CATTLE REARED IN THE AREA OF FINAL DISPOSAL OF DENPASAR) I Ketut Berata; Ni Nyoman Werdi Susari; I Made Kardena; I Nyoman Tirta Ariana
Jurnal Veteriner Vol 17 No 4 (2016)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Bali cattle are known for having high adaptability to any environmental condition and have proven tosurvive in critical environments including landfills. The aim of this study was to determine the relationshipbetween Serum Glutamic Pyruvic Transaminase /Serum Glutamic Oxaloacetic Transaminase (SGPT/SGOT) levels and lead (Pb) level in blood of bali cattle reared in the landfill Denpasar. A total of 22Balicattle were used in this study was. The blood was drawn from the jugular vein and 10 ml blood werecollected using tubes containing EDTA 0.5% and 10 mL without EDTA tubes. Levels of SGPT and SGOTwere measured by using Auto analyzer (Refloton (R) plus) method, while the measurement of Pb in bloodplasma was conducted by using atomic absorption spectrophotometry (AAS) method. Result of the SGPTand SGOT measurement obtained were relatively varied with an average of SGPT = 29.96±5.67 U/L andSGOT = 78.82±17.62.U/L. Similarly, Pb measurement results vary widely between 4.004 to 10.216 withan average of 6.595±1.85 ppm. Correlation analysis showed that there was an association between SGOTwith Pb levels. However, no correlation was observed between the SGPT with Pblevels. These resultsindicate that the levels of lead may cause pathological tissue cells in organs other than liver. Its concludedthat heavy metalPb is found in the blood of bali cattle reared in Denpasar city landfill, and it may becorrelated with the level of the SGOT and may cause pathological tissue cells organs.
Optimasi Suhu Annealing Tiga Regio Berbeda Isolat Multidrug Resistance Mycobacterium Tuberculosis dengan Metode Multiplex Polymerase Chain Reaction (ANNEALING TEMPERATURE OPTIMIZATION ON THREE DIFFERENT REGIONS OF MYCOBACTERIUM TUBERCULOSIS MULTIDRUG RESI Indra Juana Adikara; I Nengah Wirajana; Sagung Chandra Yowani
Jurnal Veteriner Vol 17 No 4 (2016)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Multiplex PCR is a method used to amplify more than one target sequences simultaneously. The aimof this research was to optimize PCR on the region of inhA promoter, inhA gene and katG gene usingMultidrug Resistance Tuberculosis (MDR-TB) Isolate. Isolation of DNA was done by using High Pure PCRTemplate Preparation Kit. Amplification process was done by Multiplex PCR usingthree pairs primers i.e.mabA-inhA-promoter-FS and mabA-inhA-promoter-R,inhA (F) and inhA (R) and KG24F and KG60R.Amplification process started by predenaturation at 95°C for 15 minutes, followed by 45 cycles consistingof denaturation at 94°C for 1 minute, annealing at 56°C, 57°C and 58°C for 1 minute and 20 seconds andextension at 72°C for 2 minutes. Then it is finished by postextension at 72°C for 10 minutes. PCR productwas detected by electrophoresis and visualized under UV Transiluminator. Annealing temperature of56°C resulted in a thicker, clearerandaccording to the desired size as compared to that of with 57°C and58°C. Conclusion that obtained was annealing temperature 56°C was optimum annealing temperatureon inhA promoter, inhA gene and katG gene region of Mycobacterium tuberculosis Multidrug Resistanceisolate using Multiplex Polymerase Chain Reaction.
Pengukuran Morfometrik Sapi Peranakan Ongole dan Kerbau Jantan dengan Metode Citra Digital (MORPHOMETRIC MEASUREMENT OF MALE ONGOLE CROSSBRED CATTLE AND BUFFALO BY DIGITAL IMAGE ANALYSIS ) Fiqy Hilmawan; Henny Nuraini; Rudy Priyanto; Bramada Winiar Putra
Jurnal Veteriner Vol 17 No 4 (2016)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

The objectives of this study were to compare the methods of animal morphometricmeasurementsusing manual and digital image analysis methods on the identify of ongole crossbred cattle and buffalo. Asmany as38 maleongole crossbred cattle and 44 male buffalos were used in this study. Fifteen bodyparameters were examined and analyzed and the data obtained were analyzed by t-student to determinethe differences between of two measurement methods. The morphometric measurement of ongole crossbredcattle and buffalo by manual was not significantly different (P>0.05) as compared to that of by digitalimaging. Therefore the digital imaging analysis method could be used as an alternative morphometricmeasurement forlarge ruminant animalal such as cattle and buffalos. Based on body morphometric bydigital image analysis showed that ongole crossbred cattle has ossa radius-ulna, os metacarpale, ossatibia fibulla, os metatarsale, hip height and body height longer than buffalo (P<0,05). Depth chest ofbuffalo deeper than ongole crossbred cattle (P<0,05). Body measurement of livestock animal by digitalanalysis method could be applied as it has similar accuracy with the manual method. Ongole crossbredcattle has good potency as beef cattle because it has higher body size proportion than buffalo.
Variasi Kolesterol Plasma Individual Monyet Ekor Panjang (Macaca fascicularis) sebagai Respons terhadap Diet Aterogenik IPB-1 [INDIVIDUAL PLASMA CHOLESTEROL VARIATION OF CYNOMOLGUS MACAQUE (MACACA FASCICULARIS) IN RESPONSE TO IPB-1 ATHEROGENIC DIET] Achmad Taher; Dedy Duryadi Solihin; Sulistiyani Sulistiyani; Dondin Sajuthi; Dewi Apri Astuti
Jurnal Veteriner Vol 17 No 4 (2016)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

The effectiveness of atherogenic diet in raising plasma cholesterol level of cynomolgus macaques(Macaca fascicularis) can be predicted for population, but not for individuals.This study aimed to evaluatethe individual plasma cholesterol of cynomolgus macaques in response to IPB-1 atherogenic diet. Theanimals under study were 22 adult malemonkeys from the animal facility of Primate Research CenterBogor Agricultural University (PSSP IPB). All animals were intervened with the IPB-1 atherogenic diet forthree months and individual plasma cholesterol wasevaluated in a monthly basis.The results showedthat the monkeys’ plasma cholesterol had increased significantly (P <0.05) after one month of interventionperiod and the increases were very significantly different (P <0.01) after three months. Individually, increasesin plasma cholesterol varied among animals. Based on these variations, 2 animals were categorised ashypo-response, 18 animals as hyper-response and 2 animals as extreme.This study showed the variationof individual plasma cholesterol of cynomolgus macaques in response tothe IPB-1 atherogenic diet so thatthe animals might be selected based on the their responsiveness.
Pemanfaatan Electronic Nose sebagai Sensor Kimiawi Urin Guna Melacak Birahi Sapi (ELECTRONIC NOSE AS URINARY CHEMICAL SENSOR FOR DETERMINING ESTROUS PHASE IN CATTLE) Pudji Astuti; Claude Mona Airin; Slamet Widiyanto; Luthfiralda Sjahfirdi; Hera Maheshwari
Jurnal Veteriner Vol 17 No 4 (2016)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

The timing of artificial insemination relies largely on behavioral observation of estrus. The problemfaced is that not all cattle shows signs of estrus significantly, which affect the accuracy of insemination,and therefore,the success rate of Artificial Insemination is less than 50%. Recently, the determination ofestradiol levels as an indicator of estrus is done by observation of physical signs and doing ELISA test,which is expensive and provide longer time. In order to solve these problems, a tool estrus detector is madenamely Electronic Nose (EN). Determination of estrus with EN is cheaper because it does not need to usecomplexmaterials, just only use the samples. Mechanism of action of EN is using a sensor that is vaporized,while animals estrus will emit pheromones that are vaporized. Theaim of this study was to determinewhether the stage of estrus can be detected by using EN. Urine of female Ongole Crossbred which maintainedin Kuwang, district of Cangkringan, Yogyakarta, with BCS of 3 was used in this research. The sample wascollected shortly before injection of dinoprost as estrus synchronization then it repeated when cattle got estrus phase. The urinary sample of the estrus cattle was sensitive to methane, propane, butane, whereasin non-estrus cattle, besides the three of these component (methane, propane, butane), sensor was alsocaptured hydrogen sulfide. Furthermore, our electronic nose had been able to distinguish estrus phase andnon-estrus based on domain area.Thus, the Electronic Nose is very prospect used as a detector of estrus incattle. Hydrogen disulfide could possibly be used as an indicator comparison between cow estrus and nonestrus.
Aktivitas Penyembuhan Luka Sediaan Topikal Ekstrak Bawang Merah (Allium cepa) terhadap Luka Sayat Kulit Mencit (Mus Musculus) (THE ACTIVITY OF TOPICAL EXTRACT OF ONIONS (ALLIUM CEPA) ON WOUND HEALING PROCESS IN MICE (MUS MUSCULUS)) Visa Yunanda; Tristia Rinanda
Jurnal Veteriner Vol 17 No 4 (2016)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Wound healing is a physiological response of the body to restore continuity, structure and function ofthe injured tissue. Onion is one of the plants that are empirically used by the community to heal wounds.The purpose of this study was to observe the effect of topical extract of onions (Allium cepa) on woundhealing process in mice (Mus musculus) strain BalbC. This study used the posttest-only control groupdesign with completely randomized design (CRD). The samples were 12 male mice with weight ranged of22-32 g divided into four treatments; i.e.: Vaseline, topical extract of onion 5%, topical extract of onion30% and topical extract of onion 55% with 3 replications. Wound healing evaluated macroscopically toobserve hyperemia, wound contraction, granulation, crusting and pus production; and microscopically bycounting the number of fibroblasts on day 10. Hyperemia, granulation, crusting and pus production wereanalyzed descriptively. Wound contraction and the number of fibroblasts were analyzed using ANOVA(p<0.05) and followed with Duncan’s test (p<0,05). Descriptive observations obtained hyperemia lasteduntil day 3, granulation in wounds used topical extract of onion 30% and 55% were faster than the other,crusting lasted until day 6 and the production of pus was not found in any treatment. Statistical testresults showed that topical extract of onion 55% significantly affected wound contraction and topicalextract of onion 30% and 55% significantly affected maturation, which characterized by the decreasednumber of fibroblasts. The results of this study showed that topical extract of onion had significant effect(p<0.05) on wound healing and the effect was in line with the increased concentration of the onion extract.
Pemberian Pakan Bahan Kering Berkuantitas Terbatas Selama Empat Minggu Tidak Menganggu Kesehatan dan Reproduksi Kambing Kacang Jantan Dewasa (FEEDING WITH A RESTRICTED QUANTITY OF DRY MATTER OVER FOUR WEEKS IS NOT DETRIMENTAL TO HEALTH AND REPRODUCTION IN Irkham Widiyono; Bambang Suwignyo; Sarmin Sarmin; Trini Susmiyati
Jurnal Veteriner Vol 17 No 4 (2016)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

The study was aimed to investigate the effects of restricted feeding for four weeks on some bloodchemistry and seminal parameters in kacang goats. Six adult clinically healthy male kacang goats wereused in this study. Each animal was kept in individual box for 12 weeks. Animal was fed with aerial partof peanut plant and concentrate with ratio of 60:40. The experiment was devided into two feeding periods,fullfeeding and restricted feeding. After four weeks adaptation (week 1-4), each animal was fullfed formaintenance plus medium activity (a quantity of dry matter at the level of 3% of body weight) for fourweeks (week 5-8) and then was fed only 50% of the fullfeeding nutritional level for the following four weeks restricted feeding period (week 9-12). During the experiment, drinking water was supplied ad libitum.Each animal was ejaculated at weekly intervals by means of standard artificial vagina for small ruminant.Blood and semen samples were collected at the end of the ad libitum and restricted feeding period for bloodchemistry and seminal parameters analyses. Statistical differences were determined by paired t-test. A0.05 probability level was used as criterion to describe statistically significant differences. Restrictedfeeding at the dry matter intake level of 1.5% body weight for four weeks did not result in significantchanging of serum glucose and total protein concentrations as well as seminal parameters. The level ofblood chemistry and seminal parameters during the feed restriction period were within the values reportedfor clinically healthy goats. It is concluded that feeding with a restricted quantity of dry matter at the levelof 1.5% of body weight for four weeks is not detrimental to health and reproduction in male kacang goats.

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