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I G. Made Krisna Erawan
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INDONESIA
Jurnal Veteriner
Published by Universitas Udayana
ISSN : 14118327     EISSN : 24775665     DOI : https://doi.org/10.19087/jveteriner
Core Subject : Health,
Jurnal Veteriner memuat naskah ilmiah dalam bidang kedokteran hewan. Naskah dapat berupa: hasil penelitian, artikel ulas balik (review), dan laporan kasus. Naskah harus asli (belum pernah dipublikasikan) dan ditulis menggunakan bahasa Indonesia atau bahasa Inggris. Naskah ilmiah yang telah diseminarkan dalam pertemuan ilmiah nasional dan internasional, hendaknya disertai dengan catatan kaki
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Articles 19 Documents
Search results for , issue "Vol 19 No 3 (2018)" : 19 Documents clear
Wabah Penyakit Ingusan (Malignant Catarrhal Fever) pada Sapi Bali di Pulau Lombok, Nusa Tenggara Barat, Indonesia (OUTBREAK OF MALIGNANT CATARRHAL FEVER IN BALI CATTLE IN LOMBOK ISLAND, WEST NUSA TENGGARA, INDONESIA) Agus Wiyono; Rini Damayanti
Jurnal Veteriner Vol 19 No 3 (2018)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Malignant catarrhal fever is a fatal disease in Bali cattle caused by Ovine Herpesvirus-2 (OvHV-2) carried by sheep. The virus has not been isolated and the vaccine is not available yet. Diagnosis in the field is observed by clinical and epidemiological changes confirmed by histopathology and antigen detection by nested polymerase chain reaction (PCR). The purpose of this paper is to provide clinical, pathological and epidemiological features as well as the results of nested PCR examination of MCF outbreaks in Bali cattle that occurred in Lombok island, West Nusa Tenggara. During January to September 2016, 20 out of 231 cattle were dead and 16 of them had depression, fever, corneal opacity, hypersalivation, purulent nasal discharges and superficial lymph nodes enlargement. There were 10 lambing sheep with the cage were located between 50 to 200 m with the cattle barn which was separated by a barrier wall. Gross lesions were characterized by diffuse hemorrhage in tracheal mucosa, epicard, abomasum, small intestine and urinary bladder and enlargement of superficial lymph nodes. Microscopiccally, pathognomonic lesions namely necrotizing vasculitis was found in the brain, trachea, cornea, lung, heart, liver, kidney and urinary bladder. Using nested PCR, OvHV-2 virus was detected in peripheral blood leucocytes/PBL in two cattle surviving from MCF and in ocular, nasal and vaginal swab from ten adult sheep. Clinical, pathological, epidemiological and molecular examinations indicate that 16 cattle were confirmed having MCF transmitted from infected adult sheep grazing and the viruses were spread by the lambing sheep kept closely to the cattle barn.
Penyuntikan Human Wharton’s Jelly Mesenchymal Stem Cells terhadap Perbaikan Fungsi Testis pada Tikus Tua Fisiologis (HUMAN WHARTON’S JELLY MESENCHYMAL STEM CELLS INJECTION AMELIORATE TESTICULAR FUNCTION ON PHYSIOLOGICAL AGING MALE RATS) Alif Iman Fitrianto; Adkhilni Utami; Wining Astini; Adisti Dwijayanti; Frans Dhyanagiri Suyatna; Kelvin Yaprianto; Indra Bachtiar; I Ketut Mudite Adnyane; Arief Boediono
Jurnal Veteriner Vol 19 No 3 (2018)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

The most common therapy on men who suffered fertility decline due to aging was called “T Therapy”, but that’s therapy has long-term risks of sexual dysfunction, metabolic syndrome, prostate, and cardiovascular system. Stem cells are an alternative therapy can be used for ameliorate testicular fuction because of their ability to differentiate into various cell types. The aim of this study was to evaluate the injection of hWJ-MSC in physiologic aging male rats on testicular function. This study was used 3 young male rats (8-12 weeks) and 6 physiological aging male rats (22-24 months) which divided into 3 groups, (i) the young rats, (ii) physiological aging male rats, and (iii) physiological aging male rats that injected with hWJ-MSCs. The young rat group did not give any treatment, physiological aging male rats received NaCl (0.9%) 0.4 mL, and the treatment group received 1x106 cells/kg BW of hWJ-MSCs. The observations were performed on the macroscopical and histological analysis. The result indicates that the younger group had the lowest body weight (154.6 g) and the percentage of the testis weight on the body weight was highest (2.2%) compared to the other groups (P>0.05). The physiological aging rats group had the smallest tubule (9726.9 ìm2) with a largest interstitial area (1117.1 ìm2) compared the other groups (P>0.05). After injection of hWJ-MSC, the tubule area became wider followed by narrowing of the interstitial area (P>0.05). The difference in the body weights is due to the different age of the rat. Improvement of tubule area and interstitial area due to the ability of hWJ-MSCs to improve spermatogenic cells within the tubule. Injection of hWJ-MSCs has been shown to increase fertility in aging rats.
Mice Oocytes Respond after Vitrification Followed by Artifical Activation Using a Various Concentration of Strontium Chloride and Cytochalasin B Muhammad Rosyid Ridlo; Rini Widyastuti; Alkaustariyah Lubis; Mokhamad Fahrudin; Arief Boediono
Jurnal Veteriner Vol 19 No 3 (2018)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Oocyte vitrification is the important part of gamete preservation for further purpose. The objective of this study was to evaluate the response or development of vitrified-mice oocyte following activation using various concentrations of Strontium Chloride (Sr Cl2). Oocytes were collected from superovulation-induced female mice. Oocytes vitrification was then performed using a gradual equilibration of 2 M Ethylene Glycol in 0.25 M sucrose and 7 M Ethylene Glycol on 0.5 sucrose. Subsequently, the vitrified oocytes were thawed and activated using various Strontium Chloride concentration in each group. Control 1 is unvitrified oocyte and without Sr Cl2. Control 2 is unvitrified oocyte then activated by 20 mM Sr Cl2. Zero (0) mM Sr Cl2 is vitrified oocyte without Sr Cl2. Group Ten (10) mM is vitrified oocyte then activated by 10 mM Sr Cl2. Group Twenty (20) mM is vitrified oocyte then activated by 20 mM Sr Cl2. The viability of vitrified-thawed oocytes was observed based on ooplasm integrity. Whereas the oocytes respond to artificial activation was observed based on pronucleus formation after 10 hours of activation. The result showed that 39% of oocyte degenerated following vitrification. The respond of vitrified-thawed oocytes following artificial activation using Strontium Chloride was significantly lower compared to fresh oocytes (p<0,05). Interestingly the highest percentage of activated oocytes (36.36%) was present in a group achieved 20 mM Strontium Chloride. As conclusion is Strontium Chloride 20mM has a best result (36,36%) to activate vitrified oocyte than 0 mM and 10 mM of Strontium Chloride.
Genetic Characterization and Bottleneck Demographic Assessment of Caspian Horse Population (KARAKTERISASI GENETIK DAN HAMBATAN DEMOGRAFI PADA PENILAIAN POPULASI KUDA KASPIA) Hamid Reza Seyedabadi; Sima Savar Sofla
Jurnal Veteriner Vol 19 No 3 (2018)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

The aim of this study was to evaluate genetic characterization of the Caspian horse population using microsatellite markers. This study was determined the efficiency of microsatellite markers for conservation plans and breeding strategies in Caspian horse population. A total of 120 Caspian horse samples including 95 adults and 25 foals were genotyped by using seventeen microsatellite markers recommended by ISAG. The number of allele per locus varied from 5 (HMS01 and HTG07) to 9 (HTG10) with an average of 7.41. The observed heterozygosity and the expected heterozygosity ranged from 0.505-0.831 (mean 0.684), from 0.615-0.835 (mean 0.748) respectively. PIC value ranged from 0.716 (HMS01) to 0.834 (AHT04) with an average of 0.787. The total exclusion probability of 17 microsatellite loci was 0.9999. The low values of Wright’s fixation index/ Fis (0.084) indicated the low levels of inbreeding. A significant heterozygote excesses based on different models, suggested that Caspian horse population has decreased to low numbers in the past, but a bottleneck event is still very striking, and its number has recently increased is not in mutation drift equilibrium. The present study contributes to our knowledge of the genetic diversity of the Caspian caspian horse population and helps to deûne its genetic conservation strategies.
Kepadatan dan Kekuatan Tulang Sapi Bali Betina yang Dipelihara Masyarakat di Bali (THE DENSITY AND STRENGTH OF FEMALE BALI CATTLE BONE REARING BY BALINESE COMMUNITY) I Wayan Batan; Betharia Criselda Fanggidae; I Ketut Suatha; I Nyoman Suarsana
Jurnal Veteriner Vol 19 No 3 (2018)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Bali cattle (Bos (Bibos) banteng) often experience fractures (fractures) of metacarpale, femur bone or tibia-fibular bone. This study was aim to reveal the strength and density of female Bali cattle bone which determines the occurrence of fractures. In order to reveal the strength of bali cattle bones, the elements that need to be explored include: bone density, cortical thickness of tibia bone, bone diameter, and bone resistance to pressure. To determine bone density performed by measuring bone specific graavity, cortical thickness of bone, carried out by splitting the bone by sawing the tibia bone extensively, then measuring the thickness of the wall of the bone cylinder using a caliper. Measurement of bone resistance to pressure was done by weighing the bone with a certain weight until the bone breaks or cracks and then measured by checking in which load the bones breaks or cracks. The result shown that the average density of Bali cow os tibia is 1.95 g/mL, average os tibia diameter is 26.45 mm, os tibia cortical thickness from top, middle, and bottom in sequence are 5.50 mm, 6.25 mm, and 5.35 mm. The result of os tibia compressive strength test is 9.26 Pascal with average load can be hold is 56.75 Newton and average wide of os tibia cross section cut is 6.05 cm2. This study result can be used as representation of Bali cow os tibia strength that has not been widely reported and can be used as recommendation for improvement of female Bali cattle livestock management.
LAKTOSA-ASTAXANTHIN MENINGKATKAN KUALITAS SEMEN BEKU KAMBING GEMBRONG DALAM UPAYA KONSERVASI Wayan Bebas; Wayan Gorda; I Gusti Ngurah Bagus Trilaksana; Desak Nyoman Dewi Indira Laksmi; Tjok Gede Oka Pemayun
Jurnal Veteriner Vol 19 No 3 (2018)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

This research was conducted to produce a formula of diluent for the manufacture of frozen semen of Gembrong goat. Yolk phosphate was used as a basic diluent with the addition of anti-cold shock, antioxidants and combination of anti-cold shock-antioxidant. The research design used a completely randomized design with three kinds of treatments; T1: the addition of lactose 0.6% (anti-cold shock), T2: the addition of astaxanthin 0.004% (antioxidant), and T3: a combination of 0.6% lactose-Astaxanthin 0.004% (combination of anti-cold shock and antioxidants). The addition of DMSO 6% was used in each treatment as intracellular cryo protectants. The freezing process was done with the gradual freezing with conventional techniques. Examination of the quality of semen by thawing prior included progressive motility, viability, and abnormalities. The results showed that anticoldshock-antioxidant combination (0.6% lactose -Astaxanthin 0.004%) produced the best semen quality with progressive motility, viability, and less abnormalities as follow 44.00 + 3.46%, 59.00 + 1.85%, and 14.00 + 0.76% respectively. It was concluded that the addition of a combination of 0.6% lactose-Astaxanthin 0.004% on the phosphate yolk diluent with 6% DMSO as intracellular produced best quality of frozen goat Gembrong semen that suitable for use in artificial insemination and in vitro fertilization.
Panjang Siklus Estrus dan Struktur Histologi Ovarium Tikus Putih Setelah Pemberian Ekstrak Etanol Daun Kaliandra Merah Kadek Mardika; Iriani Setyawati; Anak Agung Ketut Darmadi
Jurnal Veteriner Vol 19 No 3 (2018)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Red calliandra (Calliandra calothyrsus Meissn.) leaves contain chemical compounds such as flavonoids, tannins, and steroids. Previous research of C. calothyrsus leaf extracts showed effect on male mice reproduction, but no research has been done about its effect on estrous cycle and histology structure of albino rat ovary. This research aimed to study the potency of C. calothyrsus leaf extracts as phytoestrogen on the female reproductive organ through observing the estrous cycles, and histological structure and weight of the ovaries. Twenty four of 54-day-old female albino rats (Rattus norvegicus) Wistar strain were divided into four groups i.e. placebo control (given 0.5% Na-CMC solvent) and three treatment groups of C. calothyrsus leaf extracts in dosages 17.5; 35; and 70 mg/kg BW (respectively, group P1, P2 and P3). Treatments were administered orally in 1 ml/rat/day for 20 days. The vaginal smears were investigated from day 5th for 15 days (three estrous cycles). It was carried out every 8 hours/day. At the end of the treatments, rats were dissected for collecting the ovaries. They were weighed and prepared histologically then by using paraffin method with Hematoxylin-Eosin staining. The parameters observed were ovary weight and histological structures, including the number of primary follicles, secondary follicles, tertiary follicles, de Graff follicles, corpus luteum, and atretic follicles. The results showed that C. calohtyrsus leaf extracts have a phytoestrogenic potency by shortened the proestrous and estrous phases, increased the corpus luteum number and decreased the ovarian weights significantly at dose 70 mg/kg BW.
Kualitas Semen Kambing Sapera yang Dibekukan dalam Pengencer Tris Kuning Telur dengan Imbuhan Pentoxifylline (QUALITY OF SAPERA BUCK SEMEN FROZEN IN TRIS EGG YOLK EXTENDER ADDED WITH PENTOXIFYLLINE) Bq Hayyul Hidayati; Raden Iis Arifiantini; Ni Wayan Kurniani Karja; Diana Andrianita Kusumaningrum
Jurnal Veteriner Vol 19 No 3 (2018)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

The aim of this study was to determine the effective dose of pentoxyfilline (PTX) to improve the quality of sapera buck frozen semen in Tris Egg Yolk (TEY) extender. Two sexually mature sapera bucks aged 1.5 years old was used as semen source. Semen collected with artificial vagina. Fresh semen were evaluated macro and microscopically. Only ejaculates showing > 70% sperm motility were selected. Semen were divided into four aliquots tubes containing TEY extender with 0 mM, 3.5 mM, 5 mM and 6.5 mM PTX each tube. After dilution, semen were packed into mini straw (0.25 ml) and equilibrated at 5 oC for 4 hours, then frozen above liquid nitrogen vapors for 10 minutes before plunged into liquid nitrogen (-196 oC). Results of this study showed that 6.5 mM PTX addition to the TEY extender improve post-thaw semen motility (P<0.05) but not viability and plasma membrane integrity. It concluded that 6.5 mM PTX addition to TEY extender improved post-thaw sperm motility of sapera buck.
Ekstrak Daun Singkong Berpotensi Sebagai Antioksidan pada Burung Puyuh yang Mendapat Cekaman Panas Singkat La Jumadin; Aryani Sismin Satyaningtijas; Zulvia Maika; Lili Darlian; Warysatul Ummah; Koekoeh Santoso
Jurnal Veteriner Vol 19 No 3 (2018)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

The purpose of this study was to explore the potential of cassava leaves extract as an antioxidant in quail (Coturnix coturnix japonica) females as a result of heat exposure. The parameter are total erythrocyte count, hematocrit value, hemoglobin concentration and erythrocyte index (MCV, MCH, MCHC). Quails were devided into six groups. The control group (K0) was not given cassava leaves extract and heat. Treatment group (KL) was only given chlorophyll cassava leaves extract dose 5,29 mg/g. Group P was only given heat exposure. Group P+KL1, P+KL2, and P+KL3 were exposed to heat and given the doses of cassava leaves extract 5,29 mg, 10,58 mg, and 21,16 mg/168 g body weight, respectively orally for 28 days after a week of adaptation. Parameters such as the total erythrocyte count, hematocrit value, hemoglobin concentration and erythrocyte index (MCV, MCH, MCHC) were observed every two weeks. Results showed that the mean of eritrosit ranged from 8,64 x 106/mm3 in K0 to 10,47 x 106/mm3 in P+KL3. Hematocrit value in all treatment groups did not increase. Temperature exposure and paste of cassava leaves with different level of dosages were not significantly different from MCV (P>0,05). The highest level of MCV was obtained in treatment K0 (0,48 fl). The highest level of MCH ranged from 5,83-6,33 pg. The highest level of MCH was obtained in treatment P (6,33 pg). The highest level of MCHC was obtained in treatment P (63,38 %). The mean of MCHC level in quail treated with the paste of cassava leaves tended to decrease compared to those were not treated with the paste. Conclusion of this study is cassava leaf extract has potential as an antioxidant in adult quail given short heat exposure. The application of chlorophyll extracted from cassava leaves did not significantly affect the quantity of blood cells, haemoglobin level, hematocrit value and eritrosite index in adult quail treated by short thermal stress.
Ekstrak Etanol Daun Eboni Menurunkan Kadar Glukosa Darah Tikus Jantan yang Diinduksi Aloksan (ETHANOLIC EXTRACT OF EBONI LEAF DECREASE BLOOD GLUCOSE LEVEL IN ALLOXAN INDUCED MALE RATS) Khairiah Kartini; Akhmad Khumaidi; Khildah Khaerati; Ihwam Ihwan
Jurnal Veteriner Vol 19 No 3 (2018)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Eboni (Diospyros celebica Bakh.) leaf is part of the plant which used on empirically to decrease blood glucose levels. This study aims to knowing the activity of ethanol extract of ebony leaf in decrease blood glucose levels and the effective dose equivalent to glibenclamide. The study use 25 white rats male (Rattus norvegicus L.) which divided in 5 groups, that is negative control (0.5% NaCMC), positive control (glibenclamide 0.45 mg / kgBW), Etanolic Extarac Eboni Folium (EEDE) 200 mg / kgBW, EEDE 350 mg / kgBW, EEDE 500 mg / kgBW. All groups of white rats were used alloxan induction with dose 140 mg / kgBW intraperitoneal. Three days, after induction, rats with elevated blood glucose levels e”200 mg/dL were treated for 21 days orally. Blood glucose measurement was performed 5 times on days 0, 3, 10, 17, and 24. Blood glucose data was analyzed using One Way ANOVA with 95% confidence level. It is reveled that ebony leaf extract had activity in decrease blood glucose level by alloxan induction method where the effective dose equivalent to glibenclamide was dose 500 mg/kgBW.

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