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Jurnal Veteriner
Published by Universitas Udayana
ISSN : 14118327     EISSN : 24775665     DOI : https://doi.org/10.19087/jveteriner
Core Subject : Health,
Veterinary
Jurnal Veteriner memuat naskah ilmiah dalam bidang kedokteran hewan. Naskah dapat berupa: hasil penelitian, artikel ulas balik (review), dan laporan kasus. Naskah harus asli (belum pernah dipublikasikan) dan ditulis menggunakan bahasa Indonesia atau bahasa Inggris. Naskah ilmiah yang telah diseminarkan dalam pertemuan ilmiah nasional dan internasional, hendaknya disertai dengan catatan kaki
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Articles 17 Documents
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Search results for , issue "Vol 21 No 2 (2020)" : 17 Documents clear
Prevalensi Kelainan Ginjal Anjing yang Dirawat Rumah Sakit Hewan Pendidikan dan Klinik Hewan Melalui Pemeriksaan Ultrasonografi Winda Rahmania; Mokhamad Fakhrul Ulum; Siti Zaenab; Deni Noviana
Jurnal Veteriner Vol 21 No 2 (2020)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Kidney disorders frequently occured in dogs and can be examined using ultrasound. The present study was aimed to know the prevalence of abnormalities that occurs in the kidney through ultrasound examination in dogs in the Veterinary Teaching Hospital Faculty of Veterinary Medicine of IPB University and My Vets Animal Clinic Jakarta. Data from 88 dogs were taken and recapitulated based on the results of medical records through ultrasound examination. Data were analyzed descriptively using parameter of body size, age and sex. The results showed the highest prevalence of kidney disease based on body weight occurred in small breed dogs (13.6%) and large breed dogs (11.4%). Based on age the highest prevalence occurred in geriatric dog that was equal to 50%. The prevalence of renal abnormalities by sex was highest in male than female dogs (52.3%). The prevalence of kidney disorder was influenced by body weight, age, and sex. Based on correlation analysis there was a strong correlation between age and sex.
Peningkatan Performa dan Kualitas Daging Sapi Bali yang Diberi Imbuhan Tepung Jagung Dalam Ransum (MAIZE FLOUR SUPPLEMENTATION IMPROVE PERFORMANCE AND MEAT QUALITY OF BALI CATTLE) Ni Nyoman Suryani; I Wayan Suarna; I Gede Mahardika; Ni Putu Sarini
Jurnal Veteriner Vol 21 No 2 (2020)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Research has been conducted to evaluate the effect of corn flour supplementation in ration on the performance and quality of Bali beef. The study used 12 head of Bali cattle weighing 209362 kg. The experiment used a randomized block design with four treatments and and each group consisted of three replications. The four treatments are: Treatment A: Cattle given king grass feed and concentrate, Treatment B: Cows given king grass feed, concentrate and 1 kg of corn flour, Treatment C: Cows given king grass feed, concentrate and 1.5 kg corn flour and Treatment D: Cows given king grass feed, concentrate and 2 kg of corn flour. The concentrate used is commercial concentrate. Variables observed included: weight gain, feed consumption, Feed Conversion Ratio (FCR), carcass composition and quality, and meat quality. Every two weeks Bali cattle were weighed to see the weight gain. The results showed that supplementation of corn flour in the Bali cattle ration significantly (P <0.05) increase the consumption of dry matter, protein, energy and slaughter weight, and the highest was shown in treatment D respectively 7.66 kg/day; 966 g/day; 24,614 kcal/day and 389 kg. Significant increase (P <0.05) and highest also occurred in treatment D of meat fat content that is 9.66% and carbohydrate meat of 24.614%. Based on the results of this study, it can be concluded, supplementation of 1-2 kg corn flour in the Bali cattle ration can increase the growth and chemical quality of Bali beef, but it does not affect the percentage of carcass, pH, water holding capacity and cooking loss.
Keberhasilan Mendeteksi Gen Penyandi Resistensi Tetracycline dan Plasmid Mediated Quinolones pada Bakteri Salmonella Ayam di Bandung dan Purwakarta Leila Nur Aziah; Agustin Indrawati; I Wayan Teguh Wibawan
Jurnal Veteriner Vol 21 No 2 (2020)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

This study was aimed to identify genes encoding tetracycline and plasmid-mediated quinolones resistance to Salmonella spp from Poultry Farm in Bandung and Purwakarta, West Java. A total of 70 samples were collected from poultry farm in Bandung and Purwakarta, West Java. All isolates were test by selective media (Salmonella Shigella Agar/SSA) and confirmation Salmonella with polymerase chain reaction (PCR). Thirty three isolate positive from selective media Salmonella Shigella Agar (SSA) and 21 isolat was confirmed as Salmonella spp by PCR. A total of twenty one isolate isolated were tested for tetracycline, doxycicline,, nalidixic acid, oxytetracycline, enrofloxacyn using disk diffusion method. TEresistant were screened for presence of tet(A) and tet(B) genes by single PCR. The qnr(A), qnr(B) and qnr(S) genes were detected by multiplex PCR in quinolone resistant Salmonella isolates. The result of antibiotic sensitivity test showed that resistance to ampicillin (95.2%), tetracycline (100%), oxytetracycline (95.2%), nalidixic acid (90.4%), eritromisin (85.7%), enrofloxacin (76.2%), Gentamisin 47.6%, chloramphenicol (38.1%). The distribution of antibiotics-resistance genes in the Salmonella isolates included ampC (95.2%), tet(A)(61.9%), tet(B)(38.1%), qnr(A)(28.5%), qnr(B)(14.3%) and qnr(S)(23.8%). This study shows that a few pathogens of Salmonella are resistant to ampicillin, tetracycline , and quinolone. The tet and qnr genes are responsible for this resistance among Salmonella in Bandung and Purwakarta, West Java Indonesia was high.
Aktivitas Antibakteri Ekstrak Daun Pepaya, Daun Kemangi Serta Temu Ireng, dan Madu terhadap Bakteri Serratia marcescens (ANTIBACTERIAL ACTIVITY OF PAPAYA LEAVES, BASIL LEAVES AND CURCUMA AERUGINOSA EXTRACT AND HONEY AGAINST SERRATIA MARCESCENS) Yovita Devina; Vinsa Cantya Prakasita; Dwi Cahyo Budi Setiawan; Agnesia Endang Tri Hastuti Wahyuni
Jurnal Veteriner Vol 21 No 2 (2020)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Antibiotic Growth Promoters (AGPs) are antibiotics that are used commonly in livestock farming to increase animals growth rate. The use of AGP has been prohibited in Indonesia (No.14/Permentan/PK.350/ 5/2017). AGP banning urges some innovations to find the alternative of AGP and one of them is utilization of natural resources. Papaya leaves, basil leaves, Curcuma aeruginosa rhizomes and honey contain flavonoid that has antibacterial activity. The purpose of this research is to know the effect of Papaya leaves, basil leaves, Curcuma aeruginosa rhizomes and honey against Serratia marcescens growth. Re-identification of Serratia marcescens were done by looking at the colony morphology, cell morphology and biochemical tests. Antibacterial activity of ethanol (100%) and aquades (33.33%) extract of the herbals and the honey (100%) against Serratia marcescens were tested by disc diffusion method. Each test was repeated 2 times. The results showed that Lanceng (Trigona bee) honey from Gunung Kidul, Black honey from Lombok, White honey from Lombok, ethanol and aquades extract of the herbals are not effective to inhibit Serratia marcescens’s growth. Commercial honey (7.59±0.22 mm) has the highest antibacterial acivity to Serratia marcescens, followed by honey from Kupang (6.69±0.21 mm). Commercial honey and honey from Kupang have moderate antibacterial activity. It can be cocluded that comercial honey and honey from Kupang can ihibit Serratia marcescens’s growth
Penentuan Konsentrasi Susu Skim Terbaik dalam Pengencer Semen Ayam Kampung Berbahan Dasar Ringer Laktat (DETERMINATION OF OPTIMUM SKIM MILK CONCENTRATION IN KAMPUNG CHICKEN’S SEMEN EXTENDER BASED ON LACTATED RINGERS) Khaeruddin Khaeruddin; Andi Nurlinda; Abdul Hakim Fattah; Andi Kurnia Armayanti
Jurnal Veteriner Vol 21 No 2 (2020)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

The aim of this study was to determine the best concentration of skim milk in maintaining the quality of Kampung chicken’s spermatozoa during storage at 5 oC. Semen was collected from five roosters which used massage method. Furthermore, semen was divided into five tubes as treatment (no extenders, extenders containing 0%, 6%, 9% and 12% skim milk concentrations), the liquid semen was stored at 5 oC. Observations of motility, viability and abnormalities of spermatozoa were carried out at 0, 12, 24, 36, dan 48 hours. The results showed that there was a very significant difference (P <0.01) among the treatment of motility, viability and abnormalities of Kampung chicken spermatozoa after dilution during storage. Storage of spermatozoa in 6% skim milk concentrate for 48 hours was resulted in motility of 49,00±1,30%, viability of 52,13±1,53% and abnormalities of 20,47±0,47%. The use of lactated ringer extenders with a concentration of 6% skim milk can generally maintain sperm quality which was better than other treatments during storage.
Respons Kekebalan Ayam IPB D1 yang Memiliki Gen TLR4 terhadap Infeksi Bakteri Salmonella enteritidis (IMMUNE RESPONSE OF IPB D1 CHICKENS WITH TLR4 GENES AGAINST SALMONELLA ENTERITIDIS BACTERIAL INFECTION) Fitria Susanti; Sri Murtini; I Wayan Teguh Wibawan
Jurnal Veteriner Vol 21 No 2 (2020)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Faculty of Animal Science, IPB University has developed new trait of chicken named IPB D1 traits. The chicken is a crossbreed chicken between male F1 PS (Pelung x Sentul) with F1 female (Kampung x parent stock Cobb). The IPB D1 chickens aims to produce the local commercial meat type, which adapted to traditional or semi intensive management (back yard). Salmonellosis is one of the bacterial disease that commonly infected poultry, therefore a study was conducted to determine the immune response of IPB D1 chickens to these disease using genetic markers, namely TLR4 gene (Toll-Like Receptor 4). TLR4 gene is one of the genes that control chicken resistance to Gram negative bacteria infection, through non-specific immune responses, one of Gram negative bacteria is Salmonella enteritidis. The aim of this study was to determine of TLR4 gene and their role in immunity of IPB D1 chickens against S. enteritidis infection. As much as 11 chickens were use in this study. This research was carried out through several stages of i.e determination of the TLR4 gene by PCR and sequencing and observing its immune response through the number of leukocyte, leukocyte differentiation and other hematology profile. Humoral immunity response against S. enteritidis were observe by clearance test before and after challenged with S. enteritidis. The results of this study indicate that IPB D1 chickens which have TLR4 genes (genotype GG and AG) were resistance against S. enteritidis based on the leukocyte, hematology profile and humoral immunity.
Peningkatan Efikasi Vaksinasi pada Ikan Nila (Oreochromis niloticus) dengan Penambahan Ekstrak Tanaman Terung Asam dan Lempuyang Esti Handayani Hardi; Komsanah Sukarti; Maulina Anggridini
Jurnal Veteriner Vol 21 No 2 (2020)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

This research was aimed to evaluate the Solanum ferox/SF and bitter ginger (Zingiber zerumbet/ZZ) in increasing the effectiveness of the Pseudomonas sp. (EP-02) vaccine through immersion in tilapia (Oreochromis niloticus). The dose used was 600 mg.L-1 of SF; 200 mg.L-1 of ZZ and vaccine bacterial density used 108 CFU.mL-1, the ratio between vaccine and extract was 1: 1: 1. The test were begun with immersion the tilapia in the vaccine and extract for 20 minutes, the challenges test was done on day 7th (d7), 14th (d14) and 21th (d21) post-vaccination with the combined bacteria Aeromonas hydrophila (EA-01) and Pseudomonas fluorescens (EP-01) (concentration of each bacteria was 105 CFU.mL-1) through intramuscular injection of 0.1 mL each fish. Abnormal swimming patterns, pathology of anatomy, total mortality, survival rate, RPS, were measured at week 2 after the challenge test. Application of SF and ZZ extracts in vaccination can increase the non specific (haematology parameters) and specific immunity (phagocytic index and antibody) since d7 of vaccination. While the increasing fish immunity in vaccine groups occurred in d14 post vaccination. The extract administration in vaccination reduced the fish mortality; increased SR and RPS of tilapia faster than the vaccination group. In conclusion, addition of SF and ZZ extracts can increase the efficacy of vaccines faster and more effectively from day 7th of infection.
Gambaran Leukosit Setelah Pemberian Nanoenkapsulasi Andaliman (Zanthoxylum acanthopodium DC.) pada Burung Puyuh Pascainduksi Imunosupresan Deksametason Rasyida Ulfa; Akhiruddin Maddu; Huda Salahuddin Darusman; Koekoeh Santoso
Jurnal Veteriner Vol 21 No 2 (2020)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

The use of AGP (Antibiotic Growth Promoter) in animal has been banned because of the risk of antibiotic resistance. As an alternative for growth promoter. Andaliman (Zanthoxylum acanthopodium DC.) were used as feed additives. Andaliman is an herbal plant which proven has an immunomodulatory effect. However, herbal plants have low bioavailability in the body, so we need to increase its bioavaibility by reducing particle size using nanoparticle technology. The aim of our study was to determine the effects of three forms of andaliman as extracts, nanoparticles and nanoencapsulation on leukocyte differential, leukocyte count and Heterophil (H) / Lymphocyte.(L) ratio index. In this study we used 24 quails, which is divided into 4 groups, consist of.4 birds each. Group K was control group, birds were given dexamethasone (immunosuppressant), group P1 was given dexamethasone and andaliman extract, group P2 was given dexamethasone and andaliman nanoparticles, whilst group P3 was given dexamethasone and andaliman nanoencapsulation. The results showed that andaliman consumption had a significant effect (P <0.05) on differential leukocytes, number of leukocytes and H / L ratio after induction with immunosuppressants. Total leucocytes count based on the given treatment were K (8080 cell/mm3), P1 (20040 cell/mm3), P2 (20440 cell/mm3), and P3 (22040 cell/mm3). Each mean lymphocytes were K (44,6%), P1 (53,6%), P2(57,4%), and P3 (65,8%). Each mean heterophile were K (50,6%), P1 (41,8%), P2 (37,8%), and P3 (28,6%). Each mean monocytes were K (2,8%), P1 (4,6%), P2(4,8%), and P3 (5,0%). Each mean ratio index Heterophile (H) / Lymphocyte.(L) were K (1,1), P1 (0,7), P2(0,6), and P3 (0,5). It could be concluded that administration of Andaliman extract, Andaliman nanoparticles and Andaliman nanoencapsulation can increase the number of leukocytes, differential leukocytes and decrease the H / L ratio.
Analisis Filogenetik Gen Hemaglutinin dan Neuraminidase Avian Influenza H9N2 Asal Ayam Petelur di Jawa Timur (PHYLOGENETIC ANALYSIS OF HAEMMAGLUTININ AND NEURAMINIDASE GENES OF AVIAN INFLUENZA H9N2 FROM LAYER INI EAST JAVA) Prestalia Dwi Rachmawati; Tatang Santanu Adikara; Hani Plumeriastuti; Rahaju Ernawati; Jola Rahmahani; Didik Handijatno; Christian Marco Hadi Nugroho
Jurnal Veteriner Vol 21 No 2 (2020)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Avian influenza virus (AIV) subtype H9N2 is one of the infectious agents that threatens laying poultry farms, because it has an impact on drastically reducing production in the population. The aim of this study was to isolate and analyze phylogenetically the partial gene encoding the surface proteins of the AIV subtype H9N2 from laying hens in East Java. A total of 30 suspected AIV subtypes of H9N2 were taken from laying hens which had decreased production by up to 70% in three sub districts each in Kediri, Blitar and Tulung Agung regency, in East Java Province. The virus was isolated in embryonated chicken eggs and then followed by a Hemagglutination (HA) test. Detection of the presence of H9 and N2 genes was carried out through Reverse Transcriptase polymerase Chain Reaction (RT-PCR) and continued with partial gene sequencing of the two surface proteins. Data analysis was processed using BioEdit version 7.2.5 and Mega 7.0. The results show that only one sample, namely code B2.2 from Blitar regency, is an AIV subtype of H9N2. The virus in this study belong to clade h9.4.2.5 of the AIV subtype H9N2. The conclusion of this study is that the VAI subtype H9N2 was successfully isolated from laying hens in East Java and successfully identified phylogenetically.
Terapi Fotodinamik Mempercepat Kesembuhan Luka Insisi pada Kulit Tikus Putih (Rattus novergicus) yang Diinfeksi Bakteri Methicilin-Resistant Staphylococcus aureus Yonatan Dimascahyo Budianto; Lucia Tri Suwanti; Wiwik Misaco Yuniarti; Hani Plumeriastuti; Wiwiek Tyasningsih; Boedi Setiawan
Jurnal Veteriner Vol 21 No 2 (2020)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

The aims of this research was to determine the effect of photodynamic therapy (PDT) on the healing of incision wounds in rat skin (Rattus norvegicus) that infected with MRSA bacteria by evaluating and give a score (0 – 4) at wound healing factors such as epithelialization, angiogenesis, inflammatory cells, fibroblast cells and collagen density. This study used 25 white rats divided randomly into 5 groups with 5 replications, namely P0 = MRSA infection wound + Povidone Iodine, P1 = MRSA infection wound + Silver Sulfadiazine, P2 = MRSA infection wound + 5-minute PDT irradiation, P3 = MRSA infection wound + 10- minute PDT irradiation and P4 = MRSA infection wound + 15-minute PDT irradiation. Histopathological observations and scoring showed epithelialization and collagen density in groups P2, P3 and P4 increased significantly compared to groups P0 and P1 (p<0.05). The histopathological observations and scoring of angiogenesis, inflammatory cells, fibroblast cells of P0 and P1 groups had a higher score than the treatment group with photodynamic therapy P2, P3 and P4 (p<0.05). This condition showed that in the P0 and P1 groups are still in the inflammatory phase, while in the P2, P3 and P4 groups have entered the remodeling phase which is characterized by high epithelialization scores and collagen density. Giving photodynamic therapy to wounds infected with MRSA can accelerate the wound healing process.

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