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Jurnal Veteriner
Published by Universitas Udayana
ISSN : 14118327     EISSN : 24775665     DOI : https://doi.org/10.19087/jveteriner
Core Subject : Health,
Veterinary
Jurnal Veteriner memuat naskah ilmiah dalam bidang kedokteran hewan. Naskah dapat berupa: hasil penelitian, artikel ulas balik (review), dan laporan kasus. Naskah harus asli (belum pernah dipublikasikan) dan ditulis menggunakan bahasa Indonesia atau bahasa Inggris. Naskah ilmiah yang telah diseminarkan dalam pertemuan ilmiah nasional dan internasional, hendaknya disertai dengan catatan kaki
Arjuna Subject : -
Articles 18 Documents
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Search results for , issue "Vol 23 No 4 (2022)" : 18 Documents clear
Wound Healing Using White Turmeric (Curcuma zedoaria) Extract Nanoparticles: Macroscopic and Microscopic ObservationWound Healing Using White Turmeric (Curcuma zedoaria) Extract Nanoparticles: Macroscopic and Mi Fitri Ariyani; Lina Noviyanti Sutardi; Ekowati Handharyani
Jurnal Veteriner Vol 23 No 4 (2022)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19087/jveteriner.2022.23.4.441

Abstract

A wound is damaged of skin tissue caused by physical or mechanical trauma. Naturally, the wound healing process begins almost immediately after the injury occurred and is categorized by four main stage, these are hemostasis, inflammation, proliferation, and maturation. The aim of this research is to observe wound healing process using white turmeric (Curcuma zedoaria) nanoparticles extract gel based on macroscopic and microscopic observations. The skin of 24 Sprague dawley rats were cut 3 cm in the dorsal area and sutured with simple suture technique. The treatments were divided into four groups, positive control (C1), negative control (C2), gel nanoparticles white turmeric extract 0.75% (K1) and 1.5% (K2). Treatment was given every day for a week. Macroscopically observation were made descriptively on the 1st, 3rd, 5th, and 7th days. Histopatologically, the specimens were stained with hematoxylin eosin (HE) to observe the skin structure with parameters scab, epithelial formation, and neovascularization. The observations were evaluated on the 8th days post-injury. Data were presented descriptively through light microscope. The macroscopic results showed the nanoparticles extract gel treatment wound had a faster healing process and lower pain responses than the control groups. The microscopic showed the nanoparticles gel treatment extract had a better repairing process of skin structure and decreased inflammation cells than the control groups. In conclusion, the nanoparticles of white turmeric extract treatment gel group has a better results than the control groups.
Karakterisasi dan Diferensiasi Sel Punca Mesenkimal Asal Jaringan Adiposa Cokelat Monyet Ekor Panjang (Macaca fascicularis) Kartika Sari; Silmi Mariya; Irma H Suparto; Fitriya Nur Annisa Dewi; Permanawati Permanawati; Huda S Darusman; Dondin Sajuthi
Jurnal Veteriner Vol 23 No 4 (2022)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19087/jveteriner.2022.23.4.448

Abstract

Mesenchymal stem cells (MSCs) from adipose tissue, known as multipotent stem cells, is a promising source of stem cells due to its abundance and it can be easily harvested, one of these cells is brown adipose tissue-derived cells which have gained new interest the field of degenerative disease. The aim of this study was to establish MSCs culture from brown adipose tissue (BAT) of Macaca fascicularis, particularly to characterize and evaluate their differentiation ability. MSCs were isolated from BAT obtained from biopsy of three adult male M. fascicularis on scapular area. MSCs were cultured and Polymerase Chain Reaction technique was used to confirm expression of markers for MSCs. The cells were further differentiated using growth media specific for osteocytes, chondrocytes and adipocytes. The results showed that BAT-derived MSCs had fibroblast-like morphology with spindle shape. Markers for MSCs (CD73, CD90, and CD105) were expressed. Importanty, the cells were able to differentiate into osteocytes, adipocytes and chondrocytes. These results showed that BAT of M. fascicularis can be a source for MSCs; these cells may be used further as in vitro model in the studies of regenerative medicine.
Deteksi dan Analisis Filogenetik Staphylococcal Enterotoxin Y Isolat Bakteri Staphylococcus aureus Asal Kambing Peranakan Etawah Fatkhanuddin Aziz; Desy Cahya Widianingrum; Sarasati Windria; Siti Isrina Oktavia Salasia; Nurulia Hidayah; Achmad Fauzi; Fauziah Fitriana; Riza Resita
Jurnal Veteriner Vol 23 No 4 (2022)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19087/jveteriner.2022.23.4.458

Abstract

Staphylococcus aureus diketahui sering menyebabkan radang ambing pada ternak perah dan memproduksi berbagai jenis enterotoksin yang berpotensi sebagai agen penyebab keracunan makanan melalui susu maupun produk olahan susu yang dikonsumsi. Staphylococcal enterotoxin Y (SEY) asal penyakit kulit pada manusia dilaporkan tahan terhadap uji pemanasan dan menyebabkan muntah pada hewan coba primata yang menunjukkan potensinya sebagai salah satu penyebab keracunan makanan. Pada penelitian ini, deteksi gen sey pada 18 isolat S. aureus asal kambing Peranakan Etawah (PE) dengan teknik PCR, kemudian dilakukan sekuensing gen sey dari isolat S. aureus MR6. Sekuens basa nukleotida ditranslasi menjadi protein menggunakan EMBOSS Transeq (https://www.ebi.ac.uk/Tools/st/emboss_transeq/). Sekuens SEY MR6 selanjutnya dibandingkan dengan database genebank SEY dan enterotoksin lain yang berasal dari isolat S. aureus asal manusia dan sapi menggunakan MultAlin (http://multalin.toulouse.inra.fr/multalin/cgi-bin/multalin.pl). Pohon filogenetik dari protein SEY yang dibandingkan, dibuat menggunakan MEGA Software 5.0. Hasil PCR diketahui 28% isolat, positif gen sey. Analisis SEYcap dari isolat S. aureus kambing PE diketahui mempunyai persentase protein sekuens homologi sebesar 97,3% terhadap SEY asal isolat sapi perah dan manusia. Diketahui terdapat 6 posisi residu asam amino yang berbeda antar SEY yang diperbandingkan. Analisis filogenetik menunjukkan kedekatan kekerabatan dengan SEY asal manusia, namun masih 1 kluster dengan SEY asal sapi.
Aktivitas Antibakteri Ekstrak Daun Pepaya, Daun Kemangi Serta Temu Ireng, dan Madu terhadap Bakteri Serratia marcescens Agnesia Endang Tri Hastuti Wahyuni; Yovita Devina; Vinsa Cantya Prakasita; Dwi Cahyo Budi Setiawan
Jurnal Veteriner Vol 23 No 4 (2022)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19087/jveteriner.2022.23.4.465

Abstract

Antibiotic Growth Promoters (AGPs) are antibiotics that are used commonly in livestock farming to increase animals growth rate. The use of AGP has been prohibited in Indonesia (No.14/PERMENTAN/PK.350/5/2017). AGP banning urges some innovations to find the alternative of AGP and one of them is utilization of natural resources. Papaya leaves, basil leaves, Curcuma aeruginosa rhizomes and honey contain flavonoid that has antibacterial activity. The purpose of this research is to know the effect of Papaya leaves, basil leaves, Curcuma aeruginosa rhizomes and honey against Serratia marcescens growth. Re-identification of Serratia marcescens were done by looking at the colony morphology, cell morphology and biochemical tests. Antibacterial activity of ethanol (100%) and aquades (33.33%) extract of the herbals and the honey (100%) against Serratia marcescens were tested by disc diffusion method. Each test was repeated 2 times. The results showed that Lanceng (Trigona bee) honey from Gunung Kidul, Black honey from Lombok, White honey from Lombok, ethanol and aquades extract of the herbals are not effective to inhibit Serratia marcescens growth. Commercial honey (7.59±.22 mm) has the highest antibacterial acivity to Serratia marcescens, followed by honey from Kupang (6.69±.21 mm). Commercial honey and honey from Kupang have moderate antibacterial activity.
Kejadian Streptococcus suis pada Babi yang Dipotong di Rumah Pemotongan Hewan untuk Babi di Denpasar I Nengah Kerta Besung; Kadek Karang Agustina; I Gusti Ketut Suarjana; Ni Ketut Suwiti; I Gusti Ngurah Kade Mahardika
Jurnal Veteriner Vol 23 No 4 (2022)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19087/jveteriner.2022.23.4.525

Abstract

Streptococcosis cause by Streptococcus suis is an important bacterial disease because it has zoonotic potential and is capable of causing serious outbreaks in both pigs and humans. The main symptoms are meningitis, deafness, inflammation of the eyes to blindness. This study aims to find the presence of S. suis bacteria infection on slaughtered pigs in Denpasar. A total of 200 pig tonsils were taken as samples with 20 sampling times. Each sampling was taken 10 samples. The samples were grown on 5% sheep blood agar then followed by Gram staining, oxidase, coagulase, and catalase test. Suspected positive results in each collection were continued with the Polymerase Chain Reaction primers SSRecN-F and SSRecN-R tests. The results of testing on 200 samples of pig tonsils showed that 2.5% of samples were positive for S. suis. Based on the results of the study, it can be concluded that the sample of pig tonsils from the pig slaughterhouse in Denpasar was infected with S. suis.
Penanganan Luka Jeratan Kawat Baja Beserta Manajemen Perawatan Cedera pada Gajah Sumatra Liar Asal Bener Meriah, Aceh Arman Sayuti; Rian Ferdiyan; Budianto Panjaitan; Razali Daud; Christopher R. Stremme; Ridwan Ridwan; Rossa Rika Wahyuni; Rika Marwati; Mirranda Fallatanza; Mulyadi Adam; T. Armansyah TR; Rosmaidar Rosmaidar; Hafizuddin Hafizuddin
Jurnal Veteriner Vol 23 No 4 (2022)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19087/jveteriner.2022.23.4.566

Abstract

A male eight-year-old Sumatran elephant, was diagnosed with an acute accidental injury due to snare of a steel cable in the distal right front leg (radiocarpal joint). The general physiological condition of the elephant is still within normal limits and the state of the wound has not shown any severe pathological signs so that the prognosis is fausta. Management of snare wounds is carried out with a conservative approach according to the procedure. Elephant care and monitoring of recovery are carried out at the Elephant Conservation Center, at Saree, Aceh Besar. Follow-up examinations in the form of routine hematology and blood chemistry were also carried out because wound healing was past its optimal period. Laboratory examination results showed that the number of leukocytes and differential leukocytes tended to increase, while the number of platelets, erythrocytes, hemoglobin, hematocrit, total protein, albumin, and glucose tended to decrease from normal references. The results of the supporting examinations are then interpreted as a basis for better animal recovery including providing complete nutrition to animals. It can be concluded that steel wire snare wounds to the legs and the resulting injuries can be cured by treating wounds and improving the quality of feed given to Sumatran elephant patients.
Identification of Fly Larvas in White Rats (Rattus norvegicus) Carcass Treated with Various Dose of Organophospate Ichsan Luqmana Indra Putra; Maolinda Budiarti
Jurnal Veteriner Vol 23 No 4 (2022)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19087/jveteriner.2022.23.4.488

Abstract

The growth of flies on the carcass could be an indicator in estimating Post Mortem Interval. The aim of this study was to determine the type of flies and their abundance on the carcass of white rats (Rattus norvegicus) given various doses of diazinon’s organophosphate poison. This research was an experimental study using 12 female wistar white rats, aged 2-3 months, weighing 150-200 g. This study consisted of four treatments with three repetitions of each treatment. Variations in the dose of organophosphate given were treatment A (1.5 mL), B (2.5 mL), and C (3.5 mL). The parameters observed included the types and abundance of flies on the carcasses of white rats that were treated with organophosphate poisoning at different doses. Data analysis was carried out including the normality test, then continued with the homogeneity test and finally the analysis of variance test was carried out and the test results were not significantly different. The types of flies found on the carcass were Sarcophaga haemorrhoidalis, Chrysomya albiceps, C. megacephala and C. bezziana. The highest average abundance was S. haemorrhoidalis (34.3 larvae) and the lowest average abundance of fly larvae was C. bezziana (16.3 larvae). The conclusion of this study was that the variation in the dose of organophosphate does not affect the type, abundance and time of appearance of fly larvae on the carcass.
Kadar Protein Serum pada Sapi Peranakan Ongole di Balai Embrio Ternak Cipelang Bogor Putri Indah Ningtias; Sus Derthi Widhyari; Retno Wulansari
Jurnal Veteriner Vol 23 No 4 (2022)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19087/jveteriner.2022.23.4.531

Abstract

Salah satu plasma nutfah yang dimiliki oleh negara Indonesia yaitu sapi peranakan ongole (PO). Data dan nilai referensi normal kadar protein serum pada sapi PO yang digunakan sebagai donor dalam produksi embrio belum banyak dilaporkan. Tujuan penelitian ini adalah mengetahui gambaran protein serum yaitu albumin, globulin dan rasio albumin/globulin sapi PO induk yang digunakan sebagai donor sebelum produksi embrio. Penelitian ini menggunakan 10 ekor sapi PO, berumur 4-8 tahun dengan BCS 2,5-4,0. Sampel darah diambil melalui vena coccygea sebanyak 10 mL menggunakan jarum nomor 18-G. Serum dianalisis terhadap parameter protein total, albumin dan globulin dengan menggunakan alat spektrofotometer. Hasil penelitian menunjukkan bahwa rataan konsentrasi protein total, albumin, globulin, dan rasio A/G sapi donor PO masing-masing secara berurutan adalah 8,19±0,40 g/dL, 3,72±0,26 g/dL, 4,48±0,41 g/dL, dan 0,84±0,10. Data hasil penelitian ini merupakan data referensi kadar protein pada sapi PO induk. Hasil ini mendekati nilai referensi normal untuk jenis sapi yang lain. Kadar protein dapat dijadikan salah satu indikator dalam menentukan status kesehatan hewan sapi donor dalam upaya penyiapan produksi embrio.
Kajian Pustaka: Potensi Transmisi Methicillinr Resistant Staphylococcus aureus Antar Ternak dan Manusia Indah Budi Utami; Sarasati Windria; Adi Imam Cahyadi
Jurnal Veteriner Vol 23 No 4 (2022)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19087/jveteriner.2022.23.4.573

Abstract

Methicillin-resistant Staphylococcus aureus (MRSA) is a multidrug-resistant strain of S. aureus bacteria with a mortality rate range from 30 to 50% in humans. The decreased sensitivity of MRSA to vancomycin, the drug of choice for MRSA infections, makes MRSA a priority of antimicrobial-resistant pathogen for research and therapeutic development. Transmission of MRSA between animals and humans was first to be reported in the early 1970s, followed by the discovery of livestock-associated MRSA (LA-MRSA) ST-398 in 2003. Veterinarians, ranchers, and dairy farmers had higher rates of MRSA colonization due to their close relationship with livestock. The need to identify the potential transmission routes, risk factors that can cause MRSA transmission between livestock and humans, and its prevention is the basis for this research. This research was conducted by analyzing related literature obtained from online databases such as Google Scholar and PubMed, based on predetermined research criteria. Reported potential transmission routes include direct contact and airborne transmission. Several risk factors such as direct contact with livestock, antibiotic use as growth promoters, and living in areas close to livestock areas can cause MRSA transmission between livestock and humans. The use of masks, the application of good hygiene and sanitation, and screening for MRSA colonization in livestock and humans are some of the efforts that could prevent MRSA transmission between livestock and humans.
Dermatitis dan Gambaran Histologi Kulit Anjing yang Ditangani di Rumah Sakit Hewan Pendidikan Universitas Udayana Ni Ketut Suwiti; I Nengah Kerta Besung; Sri Kayati Widyastuti
Jurnal Veteriner Vol 23 No 4 (2022)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19087/jveteriner.2022.23.4.498

Abstract

Research on the incidence of dermatitis in dogs and its histopatology has been carried out. This study was aims to identify the causes of dermatitis, and to determine the histopatology of the skin of dogs suffering from dermatitis, as well as its occurrence in female and male dogs, both domestic and breed dogs that are caged or not. The study was conducted for 10 months, at the Teaching Animal Hospital, Udayana University, with a total sample of 470 individuals. Identification of the cause of dermatitis was taken from skin scrapings added with 10% kalium hydroxide (KOH) observed with a 400 times magnification of light microscope. Meanwhile, for histopatology examination, a dog skin biopsy was taken and histology preparations were made, and staining with Haematoxilin-eosin methode. Observations were made with a light microscope with 100 and 400 times magnification. The results showed that the histopatology of the skin of dogs suffering from dermatitis was found: inflammatory cells infiltration, hyperkeratosis, necrosis, hyperplasia and hydropic degeneration, and segments of Sarcoptes scabiei. The germs that cause dermatitis were identified: Aspergillus sp., Microsporum canis, M. gypseum, and Trichophyton rubrum. Dermatitis was more often found in domestic dogs (56%), adult dogs (68%) and unkept dogs (63%), while gender had no effect on the incidence of dermatitis. Conlusion of this research that, there was a similar in the histopatology feature of dermatitis dogs, caused by fungi or parasites.

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