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INDONESIA
Jurnal Veteriner
Published by Universitas Udayana
ISSN : 14118327     EISSN : 24775665     DOI : https://doi.org/10.19087/jveteriner
Core Subject : Health,
Veterinary
Jurnal Veteriner memuat naskah ilmiah dalam bidang kedokteran hewan. Naskah dapat berupa: hasil penelitian, artikel ulas balik (review), dan laporan kasus. Naskah harus asli (belum pernah dipublikasikan) dan ditulis menggunakan bahasa Indonesia atau bahasa Inggris. Naskah ilmiah yang telah diseminarkan dalam pertemuan ilmiah nasional dan internasional, hendaknya disertai dengan catatan kaki
Arjuna Subject : -
Articles 7 Documents
 1 
Search results for , issue "Vol 9 No 3 (2008)" : 7 Documents clear
AMINO-TERMINUS OF POLYMERASE BASIC-2 OF AVIAN INFLUENZA VIRUS OF H5N1 SUBTYPE ISOLATED FROM VARIOUS ANIMAL SPECIES IN INDONESIA Gusti Ayu Yuniati Kencana; Widya Asmara; Charles Rangga Tabbu; I Gusti Ngurah Kade Mahardika
Jurnal Veteriner Vol 9 No 3 (2008)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

The information on pathogenicity and adaptation factors of avian influenza virus (AIV) in mammalsis very inportant in an effort to reduce the risk of avian influenza (AI) pandemic in the future. Polymerasegene complex appears to be the major factors for adaptation of AIV to certain animal species. A preliminarystudy on role of non-coding region (NCR) and amino-terminus of polymerase-basic 2 (PB2) is presented.Purified viral RNA of AIV isolated from chicken, duck, pig, and quail of Bali and Yogyakarta was reversetranscribed into cDNA and amplified using reverse transcriptase-polymerase chain reaction (RT-PCR)using PB-2 universal forward primer and specifically designed backward primer. The result showed thatall AIV’s H5N1 isolated from chicken, duck, quail, and pig, posed PB2 amino-terminus typical for IndonesianAIV H5N1. However, polymorphic amino acids of the protein fragment did not show any species specificmotive, with the exception of the pig isolate Sw/Tabanan/2006 which had specific substitution of D16E,H17Q, M40I, and H124Y.
DETECTION OF NEWCASTLE DISEASE VIRUS BY NESTED REVERSE TRANSCRIPTASE-POLYMERASE CHAIN REACTION Anak Agung Ayu Mirah Adi; Nyoman Mantik Astawa; Ketut Santhia Adhy Putra; Yasunobu Matsumoto
Jurnal Veteriner Vol 9 No 3 (2008)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

A study to utilize nested reverse trancriptase-polymerase chain reaction (RT-PCR) for the detection ofNewcastle disease virus (NDV) infection was carried out. NDV isolated from an outbreak in KarangasemDistrict of Bali, Indonesia was propagated in chicken embryos and its pathogenicity was determined byinoculation in 3 week-old chickens. Organ samples were collected from infected chickens for nested-RTPCR.Out of several different pairs of primers designed for study, 3 pairs of primers (F4s-F6r/F5s-F5r,F8s-F10r/F8s-F8r and F12s-F14s/F13s-F13r), each of which for first-round/nested PCR, was able to amplifyspecific regions of NDV genome. In the fist-round PCR, the PCR product of 1500 bps was not clearly visiblein the agarose gel following electrophoresis. In nested PCR a PCR product of 500 bp was clearly visible onagarose gel following electrophoresis. The 3 pairs of primers appeared to be potential for an accurate andrapid detection NDV in the infected host.
Deteksi Protein Bovine Major Histocompatibility Complex Klas I dan Klas II pada Sapi Madura Ni Ketut Suwiti; Fedik Abdul Rantam; I Nengah Kerta Besung
Jurnal Veteriner Vol 9 No 3 (2008)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

A study to detect the presence of bovine major histocompatibility complex (Bo-MHC) in maduracattle has been carried out. Lymphocyte samples were collected from 25 cattle. The protein of lymphocytesamples was analysed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) andtransferred onto nitrocellulose membrane. Bo-MHC proteins on the membrane were detected by monoclonalantibodies (anti-Bo-MHC class I) and BAQ150A (anti-Bo-MHC class II). The proteins of 48 kDa and 11kDa were detected by MAb B5C which appeared to be the alpha and beta chain of Bo-MHC class I. MAbBAQ150A detected protein band of 26 KDa which was likely to be the beta chain of Bo-MHC class II. Bo-MHC class I dan class II were clearly detected in madura cattle by MAbs which provides a basis for furtherinvestigation on the role of the MHC in the susceptibility the animals to many diseases.
HYSTOPATHOLOGIC CHANGES ON AORTA OF CIRRHOSIS MALE RATS (RATTUS NORVEGICUS) INDUCTED BY ESCHERICIA COLI O55 : B5 Tony Hartono; Wiwik Misaco Yuniarti; Bambang Sektiari Lukiswanto
Jurnal Veteriner Vol 9 No 3 (2008)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

The background of this study is to visualize histopathological changes on aorta of cirrhosis rats (Rattus norvegicus) induced by endotoxin E. coli O55 : B5 This study was a laboratory experimental using complete randomized design with five treatments and five repetitions. Twenty five male Wistar rats were used as experimental model of cirrhosis by bile duct ligation (BDL) technique. Three weeks after BDL, all cirrhosis experimental models were induced with a single intra venous injection of Eschericia coli endotoxin (3mg/kg b.b in 1 ml sterile saline), except those of five control rats that induced with sterile saline at the same volume only. Aortas of control rats group were excised at 6 hours after induction with sterile saline, whereas the other four groups were done at 6, 12, 18 and 24 hours after induction with endotoxin. The quantity of endothelial cell, discontinuity increment and the thickness of internal elastic lamina layer were observed to know histopathological changes on aorta. Histopathological changes were observed using a light mycroscope, dyscontinuity and thickness of internal elastic lamina were measured by reticular micrometer. The quantity of endothelial cell on control and observation interval of 6 and 12 hours as significant difference (P<0.05), which are bigger than that of 18 and 24 hours. Rats in the control group have the biggest quantity comparing to the other treatments. Discontinuity and thickness of internal elastic lamina layer had significant difference (P<0.05) on control, observation on 6 and 12 hours compared to observation on 18 and 24 hours after being induced with endotoxine. The highest discontinuity and the thinnest elastic lamina internal were obtained within observation on 24 hours. VCAM-1 expression on control group differ from observation on 6 and 12hours but all of them have significant difference to observation on 18 and 24 hours (P<0,05). The decrease of endothelial cell number is caused by endothelial cell contact with endotoxin. Longer contact interval can make more severe injury and dysfunction. When the aorta had loss of its endothelial cell, internal elastic lamina will exposed with endotoxin directly. In the vessel, endotoxin cause nitric oxide (NO) release, that have antiproliferative characteristic for neointimal formation. This condition presumed to be the cause of the dyscontinuity increment and thickness decrement of internal elastic lamina of cirrhosis rats. VCAM-1 expression was influenced by quantity and condition of endothelial cell. The higher VCAM-1 expression in control group higher than the other group because the quntity and morphofunction of endothelial cell was better than that of 6, 12, 18 and 24 hours.
PHYLOGENETIC AND ANTIGENIC STRUCTURE OF AVIAN INFLUENZA VIRUS OF H5N1 SUBTYPE ISOLATED FROM WATERFOWLS R Susanti; Retno Damajanti Soejoedono; I Gusti Ngurah Kade Mahardika; I Wayan Teguh Wibawan; Maggy Thenawidjaja Suhartono
Jurnal Veteriner Vol 9 No 3 (2008)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

A study was carried (1) to analyze the phylogenetic relationship of fragment hemaglutinin (HA) geneof avian influenza viruses (AIV) subtype H5N1 isolated from apparently healthy backyard waterfowls inWest Java with representative of animal and human isolates from Indonesia and some countries in Asia;(2) to find out cross-reactivity of those viruses with a standard Indonesian strain. Nucleotide sequences ofHA gene of AIV H5N1 from backyard waterfowls along with other H5N1 isolates of Indonesian and Asianorigin were aligned using with ClustalW of MEGA 3.1 program. Estimation of genetic distance and theconstruction phylogenetic tree were conducted by Neighbor Joining method and calculation of distancematrix using Kimura 2-parameter. Antigenic analysis was conducted using hemagglutination inhibition(HI) test. Result of phylogenetic analysis indicated that all viruses from backyard waterfowls form threedistinct sublineages. One lineage was located in Indonesia cluster and two lineages in Asia cluster. In thephylogenetic analysis, it was concluded that multiple introductions of AIV H5N1 to Indonesia have occurred.Six AI H5N1 viruses from backyard waterfowls (IPB1-RS to IPB6-RS) appeared to be different ancestorsthose isolated previously in Indonesia. Cross-antigenic analysis showed that nine viruses isolates used inthis study were antigenically different to Legok 2003 chicken strain of AIV H5N1. The HI titer of anti-Legok 2003 antibody with all newly isolated viruses is up to 6 log lower then the HI titer using homologstrain.
TRAP PRODUCTION AND REDUCTION LARVAE III HAEMONCHUS CONTORTUS BY NEMATOPHAGOUS MOULDS Riza Zainuddin Ahmad
Jurnal Veteriner Vol 9 No 3 (2008)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

A study was carried out to determine the ability of nematophagous moulds (Arthrobotrys oligosporaand Duddingtonia flagrans) to trap and reduce the number of H. contortus larvae III. Tests was conducted inpetri dishes containing agar medium, the moulds and Haemonchus contortus larvae. The ability of bothmould in trapping H. contortus larvae III was observed 3; 4; 10; 24 and 48 hours post-incubation, whereas theability of both mould in killing the larvae was observed 0,5; 3; 6; 9; 12; 15; 21; 24; 27 and 48 hours postincubation.The results showed that A. oligospora was more capable in trapping and reducing H. contortuslarvae III than Duddingtonia flagrans (p<0, 01). It was also evident that A. oligospora of Denmark originwas more capable in trapping and killing Haemonchus larve than that of local isolate. It is clear from thisstudy that A. oligospora is potential biological method for controling H. contortus infection in animals.
THE DEVELOPMENT OF FOLLICLES AND OOCYTES VIABILITY FROM EWE OVARIUM POST-INTRAUTERINE TRANSPLANTATION TO PSEUDOPREGNANT RABBIT Ramadhan Sumarmin; Adi Winarto; Tutty Laswardi Yusuf; Arief Boediono
Jurnal Veteriner Vol 9 No 3 (2008)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

The objective of this study was to evaluate the development of ewe follicles and oocytes viabilityfollowing an intrauterine transplantation of the ovaries into pseudopregnant rabbit. The experiment wasconducted in day 1 of pseudopregnant rabbit. Following transplantation for 5, 7, and 9 days, the ovarieswere recollected. The development of follicles determined by counting the number of follicles in paraffinembedded ovaries after staining with haematoxylin-eosin (HE). The viability of oocytes was determined byslicing the ovaries. Oocytes were incubated in CO2 incubator with 5% CO2, 38°C for 24 hours. After maturation,the oocytes were stained with 2% aceto-orcein to determine the nuclear oocytes status. The result showedthat follicles were detected in all stages of their development (primordial, primary, preantral, and antralfollicle stages), but their number decreased significantly (P<0.05) 5, 7 or 9 days after transplantation,except for those at primordial stage which at day 5 post-transplantation (634.7±56.88) were not significantlydifferent to the control (683.7±61.55). After maturation, the oocytes that were able to reach the M-II phaseat day 5 and day 7 post-transplantation were 35.05% and 35.24% respectively. They were significantly(P<0.05) lower than the control (56.65%). In conclusion, the development of follicles and oocytes viability inthe ewe ovaries in pseudopregnant rabbits was still preserved during intrauterine transplantation.

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