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All Journal Jurnal Crystal : Publikasi Penelitian Kimia dan Terapannya
Zahrotun Nafisah
Program Studi Kimia, FMIPA, Universitas Palangka Raya
Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering

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UJI AKTIVITAS BROMELIN DARI ANANAS COMOSUS DAN PAPAIN DARI CARICA PAPAYA TERHADAP PH MENGGUNAKAN METODE SPEKTROFOTOMETRI UV-VIS Zahrotun Nafisah; Dermawan Saro Halawa; Eka Miranda Silaban; Sonia Tri Mart Hutapea; Nasa Natalia; Nia Hana Pratiwi; Kamelia Fahmiati
Jurnal Crystal : Publikasi Penelitian Kimia dan Terapannya Vol. 8 No. 1 (2026): Literasi Artikel Penelitian Kimia
Publisher : Program Studi Kimia, Fakultas MIPA, Universitas PGRI Banyuwangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36526/jc.v8i1.7624

Abstract

Protease enzymes such as bromelain from Ananas comosus and papain from Carica papaya are cysteine ​​protease enzymes that have great potential in various applications in the food, pharmaceutical, and biotechnology industries. This study aims to characterize the activity of bromelain and papain enzymes against pH variations using a casein-based spectrophotometric method and a tyrosine standard curve. Enzyme activity is determined based on the amount of tyrosine produced from casein hydrolysis and measured at a wavelength of 280 nm. One unit of enzyme activity is defined as the amount of enzyme that produces 1 µmol of tyrosine per mL per unit reaction time. The yields of bromelain and papain enzyme extraction in this study reached 38% and 32% with protein levels of 10.04 and 21.89 mg/mL, respectively. The activity values ​​of bromelain and papain are significantly affected by changes in pH. Bromelain showed optimum activity at pH 6, amounting to 25.4 U/mL, while the activity decreased drastically at acidic pH. The papain enzyme also has an optimum activity of 17.1 U/mL at pH 6, which decreases drastically at alkaline pH. This activity pattern is consistent with the general characteristics of cysteine ​​protease enzymes, which are sensitive to changes in the ionization of their active groups. The differences in response to pH variations indicate the specific characteristics of each enzyme, influenced by the structure and conformational stability of its protein. The results of this study provide important information regarding the optimum working conditions of both enzymes as a basis for developing industrial applications based on local biological resources.
Co-Authors Dermawan Saro Halawa Eka Miranda Silaban Kamelia Fahmiati Nasa Natalia Nia Hana Pratiwi Sonia Tri Mart Hutapea