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All Journal Prisma Sains: Jurnal Pengkajian Ilmu dan Pembelajaran Matematika dan IPA IKIP Mataram
Kartiani, Elvina
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Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Education Electrical & Electronics Engineering Energy Engineering Environmental Science Mathematics Physics Other

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Study Biopotency of Rambusa (Passiflora foetida L.) Fruit Extract As An Antibacterial for Aeromonas hydrophila Fatmawati, Fatmawati; Hanafie, Agussyarif; Aisiah, Siti; Rini, Ririen Kartika; Olga, Olga; Agustina, Rizka; Aminah, Aminah; Kartiani, Elvina
Prisma Sains : Jurnal Pengkajian Ilmu dan Pembelajaran Matematika dan IPA IKIP Mataram Vol. 14 No. 2: April 2026
Publisher : Universitas Pendidikan Mandalika

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/j-ps.v14i2.15708

Abstract

Motile Aeromonad Septicemia (MAS) is a disease affecting freshwater fish and is caused by Aeromonas hydrophila. The control of MAS commonly relies on synthetic drugs and antibiotics. This study aimed to evaluate the biopotential of rambusa (Passiflora foetida L.) fruit extract as an antibacterial agent against A. hydrophila. The antibacterial activity was assessed using the disc diffusion and Minimum Inhibitory Concentration (MIC) methods, supported by qualitative and quantitative phytochemical analyses. The results showed that rambusa fruit extract produced inhibition zones against A. hydrophila. The inhibition zone of the aqueous extract after 24 hours of incubation was 3.25 mm, while the methanolic extract produced an inhibition zone of 11.42 mm. These findings indicate that rambusa fruit extract prepared using both distilled water and methanol as solvents can inhibit the growth of A. hydrophila. Qualitative phytochemical screening of the aqueous extract revealed the presence of flavonoids, alkaloids, tannins, saponins, and hydroquinones based on the 10% KOH test. Quantitative analysis also detected flavonoids, alkaloids, tannins, saponins, steroids, phenolics, anthocyanins, and triterpenoids. The MIC test showed that concentrations of 100–600 mg/L were effective in inhibiting the growth of A. hydrophila within the weak inhibition category, while concentrations of 700–900 ppm showed higher inhibitory activity. These results suggest that rambusa fruit extract has potential as a natural antibacterial agent against A. hydrophila.
Co-Authors Agussyarif Hanafie Agustina, Rizka Aminah Aminah Fatmawati, Fatmawati Olga Olga Ririen Kartika Rini Siti Aisiah