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Tsany, Haura Fikriyyah
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology

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Comparative Analysis of DNA Barcodes in The Fabaceae Family Tsany, Haura Fikriyyah; Indriyani Roslim, Dewi
Jurnal Biologi Tropis Vol. 26 No. 2 (2026): April - Juni
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v26i2.11696

Abstract

In identifying plants, people are confused about choosing the appropriate barcode for identification. For that reason, this study aims to analyze four DNA barcode rbcL, matK, trnL-trnF IGS, and ITS in the Fabaceae family. DNA sequence were taken and collected from Genbank through the NCBI website, which is 30 species from 6 genera each consisting of 5 species and the outgroup (Acacia auriculiformis). DNA sequence analysis was performed using MEGA 11. Phylogenetic analysis were performed using the Neighbor Joing Tree (NJ) and Maximum Likehood (ML) methods. The results of the analysis sequence variation showed that the highest conserved site was found in rbcL (83.72%) and variable site was found in ITS (62.03%). The number of segregation sites, substitution rates and diversity values in the Fabaceae family were highest in the ITS, respectively (451), (0.155284), and (0.219148). In conclusion, the four DNA barcodes analyzed in the Fabaceae family, namely rbcL, matK, trnL-trnF IGS and ITS, were able to separate the genera in the Fabaceae family. Among the four DNA barcodes analyzed, ITS was superior in identifying the Fabaceae family. The results of this study can be used as information in identifying plants in the Fabaceae family.
Co-Authors Dewi Indriyani Roslim