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Improving Seed Viability and Vigor of Acacia crassicarpa A. Cunn. Ex Benth. Using Ultrafine Bubbles and Biopriming Based on Dark Septate Endophytes Dede Jajat Sudrajat; Evayusvita Rustam; Mohammad Agus Salim; Yulianti; Nurhasybi; Naning Yuniarti; Surono; Yohanes Aris Purwanto
Jurnal Sylva Lestari Vol. 14 No. 2 (2026): May
Publisher : Department of Forestry, Faculty of Agriculture, University of Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jsl.v14i2.1446

Abstract

Acacia crassicarpa is a fast-growing tree species that has been a mainstay in the establishment of industrial plantation forests in Indonesia. The need for A. crassicarpa seedlings for large-scale planting programs is often hampered by poor seed germination due to strong dormancy and low seed vigor. This study aims to analyze the effects of ultrafine bubbles (UFB) and biopriming with dark septate endophytes (DSE) on the viability and vigor of A. crassicarpa seeds. Before treatment, all seeds were soaked in 98% H2SO4 for 15 minutes as a field procedure for A. crassicarpa seed germination, serving as the positive control in this study. A completely randomized factorial design with two factors, i.e., UFB water (0, 8, and 20 ppm) and DSE isolates (without DSE, MM15 (DSE MM.15), Cladosporium teunissimum (DSE KSP.1), and Dendrothyrium sp. (DSE CPP.114), was used to test the effects of the treatments on seed germination parameters (germination capacity, germination speed, germination value, hypocotyl and radicle length, and vigor index). The germination parameters were analyzed using analysis of variance and Duncan’s Multiple Range Test at the 95% confidence level. The interaction between UFB and DSE treatments significantly affected germination capacity, germination speed, germination value, and vigor index. The optimal result was achieved by soaking seeds in 8 ppm UFB water for 24 hours, combined with soaking in DSE CPP.114 for 24 hours with a germination capacity of 78.5% (increase of 32.5% from control), germination speed of 8.04% day-1 (increase of 38% from control), germination value of 15.39 (increase of 188.7% from control), and vigor index of 11.29 (increase of 80.3% from control). Thus, the UFB water and DSE Dendrothyrium sp. (DSE CPP.114) can increase seed viability and vigor after scarification, thereby directly improving the quality and quantity of A. crassicarpa seedlings for planting programs. Keywords: Acacia crassicarpa, biopriming, dark septate endophyte, seed germination, ultrafine bubbles
Effects of Semi-Automated Preprocessing in The Beef Freshness Prediction based on Near Infrared Spectroscopy Ridwan Raafi'udin; Yohanes Aris Purwanto; Imas Sukaesih Sitanggang; Dewi Apri Astuti
Simetris: Jurnal Teknik Mesin, Elektro dan Ilmu Komputer Vol. 16 No. 2 (2025): JURNAL SIMETRIS VOLUME 16 NO 2 TAHUN 2025
Publisher : Fakultas Teknik Universitas Muria Kudus

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24176/simet.v16i2.15142

Abstract

This study investigates the application of near-infrared spectroscopy (NIR) within the wavelength range of 1350–2550 nm to predict key quality parameters of beef, specifically focusing on tenderloin cuts. The quality indicators assessed include drip loss, color, pH, moisture content, storage duration, and total plate count (TPC) as a measure of microbial load. Predictive modeling was conducted using three machine learning algorithms: Partial Least Squares (PLS), Support Vector Regression (SVR), and Random Forest Regressor (RFR). To enhance model accuracy, a semi-automated preprocessing pipeline was employed utilizing the Nippy library. This library integrates several spectral preprocessing techniques including Savitzky-Golay filtering, Standard Normal Variate (SNV), Robust Normal Variate (RNV), Local Standard Normal Variate (LSNV), as well as clipping, resampling, baseline correction, and smoothing.  Among the models developed using raw spectral data, the RFR model exhibited the highest performance, achieving coefficient of determination (R²) values of 0.82 for drip loss, 0.65 for color, 0.67 for pH, 0.61 for moisture content, 0.81 for storage duration, and 0.76 for TPC. Post preprocessing, the predictive accuracy improved significantly with R² values increasing to 0.89, 0.82, 0.87, 0.85, 0.91, and 0.90 respectively for the same parameters. These findings underscore the potential of combining advanced machine learning techniques with robust preprocessing methods to enhance the non-destructive, rapid assessment of beef quality parameters. This approach offers a promising tool for quality control in the meat processing industry, facilitating more efficient and accurate monitoring of product standards.