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p-Index From 2021 - 2026
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All Journal Journal of Stem Cell Research and Tissue Engineering
Depi Lusianti
Dental Engineering Study Program, Hang Tuah School of Health Sciences
Engineering Health Professions Immunology & microbiology Medicine & Pharmacology Veterinary

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THE EFFECTIVENESS OF STEM CELL SECRETOME ON POST-TOOTH EXTRACTION WOUND HEALING Depi Lusianti; Wasis Syahwanda
Journal of Stem Cell Research and Tissue Engineering Vol. 10 No. 1 (2026): JOURNAL OF STEM CELL RESEARCH AND TISSUE ENGINEERING
Publisher : Stem Cell Research and Development Center, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jscrte.v10i1.92273

Abstract

Tooth extraction is a common surgical procedure performed in dental practice and may cause damage to both soft tissue and alveolar bone. The post-tooth extraction wound healing process involves various biological mechanisms, including inflammation, cell proliferation, angiogenesis, and tissue remodeling. In recent years, stem cell secretome has emerged as a promising regenerative therapy approach because it contains various bioactive factors such as growth factors, cytokines, and extracellular vesicles that play important roles in tissue repair. This study aimed to evaluate the effectiveness of stem cell secretome on post-tooth extraction wound healing through a systematic review method. Literature searching was conducted using the PubMed, Google Scholar, and ScienceDirect databases within the 2020–2026 publication period. Articles were selected based on inclusion and exclusion criteria according to the PRISMA guidelines. The review results showed that stem cell secretome was able to enhance fibroblast proliferation, angiogenesis, collagen deposition, wound epithelialization, and alveolar bone regeneration. In addition, secretome also played a role in reducing inflammatory responses, thereby accelerating the oral wound healing process. Based on the results of this systematic review, stem cell secretome has effective potential as a regenerative therapy to accelerate post-tooth extraction wound healing. However, further clinical studies are still needed to confirm its effectiveness and safety in humans.
OPTIMIZATION OF CULTURE AND CHARACTERIZATION METHODS OF DENTAL PULP STEM CELLS FOR REGENERATIVE APPLICATIONS IN DENTISTRY Yulda Ningsih; Depi Lusianti
Journal of Stem Cell Research and Tissue Engineering Vol. 10 No. 1 (2026): JOURNAL OF STEM CELL RESEARCH AND TISSUE ENGINEERING
Publisher : Stem Cell Research and Development Center, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jscrte.v10i1.92294

Abstract

Dental pulp stem cells (DPSCs) are a type of mesenchymal stem cell with high potential in the field of regenerative dentistry. Their successful application is greatly influenced by the methods used for isolation, culture, and characterization; however, to date, there is no fully agreed-upon standard. This article aims to review and optimize the methods of isolation, culture, and characterization of DPSCs based on the latest scientific literature in order to obtain the most effective and consistent approach for laboratory and regenerative application. This article is a methodological paper based on a literature review (2020–2025) from PubMed and Google Scholar. The protocol was developed through the synthesis of various studies related to enzymatic isolation and the explant method, cell culture using DMEM or α-MEM media, and characterization using flow cytometry and immunocytochemistry. The analysis was conducted descriptively and narratively to compare the effectiveness of each method. The enzymatic method demonstrates higher efficiency in cell isolation, whereas the explant method provides more stable cell viability. Cell culture using media supplemented with fetal bovine serum (FBS) under conditions of 37°C and 5% CO₂ supports optimal proliferation. Characterization using markers CD73, CD90, and CD105 (positive), as well as CD34 and CD45 (negative), constitutes the primary standard for DPSC identification. The combination of enzymatic isolation methods, controlled culture using standard media, and characterization based on flow cytometry represents the most optimal approach for producing high-quality DPSCs for regenerative dentistry applications.
Co-Authors Wasis Syahwanda Yulda Ningsih