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All Journal Journal of Stem Cell Research and Tissue Engineering
Parti Putri
Medical Laboratory Technology, Harapan Bangsa Academy of Health Analyst
Engineering Health Professions Immunology & microbiology Medicine & Pharmacology Veterinary

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ANTIBACTERIAL EFFECTS OF MESENCHYMAL STEM CELL SECRETOME ON THE GROWTH OF PATHOGENIC BACTERIA Parti Putri; Tri Juanda
Journal of Stem Cell Research and Tissue Engineering Vol. 10 No. 1 (2026): JOURNAL OF STEM CELL RESEARCH AND TISSUE ENGINEERING
Publisher : Stem Cell Research and Development Center, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jscrte.v10i1.92276

Abstract

Bacterial infection remains a major global health problem due to the increasing emergence of antibiotic-resistant microorganisms. Mesenchymal stem cell (MSC) secretome has attracted attention as a potential cell-free therapeutic approach because it contains bioactive molecules with antimicrobial, immunomodulatory, and regenerative properties. This study aimed to evaluate the antibacterial activity of MSC secretome against pathogenic bacteria in vitro. This experimental laboratory study used a post-test only control group design. Human dental pulp-derived MSCs were cultured under standard conditions, and conditioned medium containing secretome was collected and processed. Antibacterial activity against Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa was evaluated using agar diffusion and bacterial viability assays. Experimental groups included control, low-, medium-, and high-concentration secretome treatments. Inhibition zone diameters and Log10 CFU/mL values were analyzed using one-way ANOVA followed by Tukey post hoc test with a significance level of p < 0.05. The results showed that MSC secretome significantly inhibited bacterial growth compared with the control group. Antibacterial activity increased proportionally with secretome concentration, as demonstrated by larger inhibition zones and lower bacterial viability values. The highest antibacterial activity was observed in the high-concentration secretome group, which reduced bacterial viability by approximately 70–80%. Staphylococcus aureus showed greater susceptibility than Gram-negative bacteria, particularly Pseudomonas aeruginosa. Statistical analysis confirmed significant differences among treatment groups (p < 0.05). In conclusion, MSC secretome demonstrated significant concentration-dependent antibacterial activity against pathogenic bacteria in vitro. These findings suggest that MSC secretome may serve as a promising cell-free therapeutic strategy for infectious disease management and regenerative medicine.
EFFECT OF FETAL BOVINE SERUM (FBS) CONCENTRATION ON THE VIABILITY AND PROLIFERATION OF MESENCHYMAL STEM CELLS (MSCS) IN VITRO Tri Juanda; Parti Putri
Journal of Stem Cell Research and Tissue Engineering Vol. 10 No. 1 (2026): JOURNAL OF STEM CELL RESEARCH AND TISSUE ENGINEERING
Publisher : Stem Cell Research and Development Center, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jscrte.v10i1.92278

Abstract

Mesenchymal Stem Cells (MSCs) are multipotent stem cells widely utilized in regenerative medicine due to their self-renewal and differentiation capacities. The success of MSC expansion in vitro is strongly influenced by culture conditions, particularly the concentration of Fetal Bovine Serum (FBS) used in the culture medium. This study aimed to evaluate the effect of different FBS concentrations on the viability and proliferation of MSCs in vitro. An experimental laboratory study with a post-test only control group design was conducted using MSC cultures supplemented with 0%, 5%, 10%, and 15% FBS in Dulbecco’s Modified Eagle Medium (DMEM). Cells were incubated for 72 hours under standard culture conditions. Cell viability was assessed using the MTT assay, while proliferation was analyzed using trypan blue exclusion and hemocytometer counting. Statistical analysis was performed using One-Way ANOVA followed by Tukey post hoc test with a significance level of p < 0.05. The results demonstrated that FBS concentration significantly affected MSC viability and proliferation. MSCs cultured with 10% FBS exhibited the highest viability, proliferation rate, and optimal spindle-shaped fibroblast-like morphology with high confluency. In contrast, cells cultured without FBS showed poor attachment, reduced proliferation, and increased apoptotic features. Although the 15% FBS group maintained relatively high viability, proliferation was slightly lower compared to the 10% FBS group. In conclusion, FBS concentration plays a critical role in maintaining MSC growth and survival in vitro, with 10% FBS providing the most optimal culture condition among the tested groups.
Co-Authors Tri Juanda