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EFFECTS OF PRETREATMENT METHODS ON THE LIGNOCELLULOSIC COMPOSITION OF SALACCA MIDRIBS Berlian Simanjuntak; Isabella Kristin Hotma Silalahi
BIOMA : Jurnal Ilmiah Biologi Vol. 15 No. 1 (2026): April 2026
Publisher : Prodi Pendidikan Biologi, FPMIPATI, Universitas PGRI Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26877/bioma.v15i1.3473

Abstract

This study aims to determine the effect of pretreatment on hemicellulose, cellulose, and lignin content of Salacca midrib. Unlike previous studies, this study was designed to evaluate the effects of single-, double-, and triple-stage pretreatments on the sallaca midrib. The study was also designed to evaluate the differences in the physical and biological pretreatment methods. The pretreatments applied consist of physical, chemical, biological, physicochemical, chemical-biological, physical-biological, and physicochemical-biological pretreatments. Physical pretreatment is carried out in an autoclave at 121 °C, chemical pretreatment using Sodium Hydroxide (NaOH) 4%, and biological pretreatment using Debaryomyces hansenii. Based on the ANOVA test, the various pretreatment methods have a significant effect on lignocellulose content. The highest hemicellulose content was the chemical pretreatment at 34.13%, the cellulose content was the chemical pretreatment at 40.85%, and the highest lignin reduction was the physical-chemical-biological at 27.15%
PELATIHAN DAN PENDAMPINGAN PRAKTIKUM MIKROBIOLOGI BAGI SISWA DAN GURU BIOLOGI TINGKAT SMA, KABUPATEN TOBA Berlian Simanjuntak; Roga Florida Kembaren; Merry Meryam Martgrita; Chintya Sinar Lumbantoruan; FBA Fitriani; Theodora M Putri; Adrianto Prihartantyo; Yulisa Lestari; Juli Yanti Damanik; Andrew Rolas Siagian; Endang Sibarani; Bastian Jose Habeahan; Reni Tamba; Ketty Tambunan; Elsa Pangaribuan; Gabriele Siagian
Jurnal Abdi Insani Vol 13 No 6 (2026): Jurnal Abdi Insani
Publisher : Universitas Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/abdiinsani.v13i6.3922

Abstract

The training and mentoring of microorganism isolation and identification practicums wereconducted as an effort to provide learning integration experiences with practicum activitiesdue to the limited laboratory facilities in high schools in Toba Regency. The community serviceactivities began with an evaluation of initial understanding related to the community serviceactivities carried out, followed by the provision of basic theory on microorganism isolation andidentification. After that continued with practical activities of isolation and identification ofmicroorganisms carried out through aseptic techniques and mentoring in observations, datarecording, and analysis of practical observation results. The activity was concluded with a finalevaluation of the activity. Training and mentoring were carried out intensively so the studentsand teachers were able to carry out the stages and analysis of practical activities appropriately.The evaluation results showed that this activity increased the understanding of students andteachers of the basic theory and practical work of isolating and identifying microorganisms for66%. This training activity is also expected to be a strategic step to encourage high schools inToba Regency to have laboratory facilities to support the integration of learning materials withpractical activities.
Pengaruh Metode Ko-Inokulasi Bakteri Asam Laktat dan Yeast terhadap Karakterisasi dan Atribut Aroma Tuak Berlian Simanjuntak; Theodora M Putri; Rachel Sinaga; Michael Sipayung
Jurnal Teknik Kimia USU Vol. 15 No. 2 (2026): Jurnal Teknik Kimia USU
Publisher : Talenta Publisher (Universitas Sumatera Utara)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32734/jtk.v15i2.25740

Abstract

In previous research, the Friedman test on the aroma of tuak with the addition of suspected as Lactic Acid Bacteria (LAB), yeast, and a consortium showed no significant difference among the samples. However, tuak with suspected as LAB was preferred by the panelists. Due to the panelists inability to determine specific aromas, this study was conducted to identify the specific aroma attributes from the fermentation of palm sap. Following this, a multiple comparison test and a hedonic test were performed to determine if the coinoculation technique improves the aroma quality of tuak. The results of the descriptive test were consistent with the ANOVA test results, showing that the aroma of tuak with co-inoculation differed from tuak without co-inoculation. Tuak with co-inoculation detected the aroma like ethyl lactate, while tuak without co-inoculation had an aroma like lactic acid. In the multiple comparison test, the aroma of co-inoculated tuak was better with a moderate level of difference. The hedonic test showed that panelists preferred the aroma of co-inoculated tuak, specifically with a yeast to suspected as LAB ratio of 1:2. The alcohol levels of the tuak under these conditions were 0.94% ± 0.06 with pH 3.95 ± 0.03.
Pengaruh Metode Ko-Inokulasi Bakteri Asam Laktat dan Yeast terhadap Karakterisasi dan Atribut Aroma Tuak Berlian Simanjuntak; Theodora M Putri; Rachel Sinaga; Michael Sipayung
Jurnal Teknik Kimia USU Vol. 15 No. 2 (2026): Jurnal Teknik Kimia USU
Publisher : Talenta Publisher (Universitas Sumatera Utara)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32734/jtk.v15i2.25740

Abstract

In previous research, the Friedman test on the aroma of tuak with the addition of suspected as Lactic Acid Bacteria (LAB), yeast, and a consortium showed no significant difference among the samples. However, tuak with suspected as LAB was preferred by the panelists. Due to the panelists inability to determine specific aromas, this study was conducted to identify the specific aroma attributes from the fermentation of palm sap. Following this, a multiple comparison test and a hedonic test were performed to determine if the coinoculation technique improves the aroma quality of tuak. The results of the descriptive test were consistent with the ANOVA test results, showing that the aroma of tuak with co-inoculation differed from tuak without co-inoculation. Tuak with co-inoculation detected the aroma like ethyl lactate, while tuak without co-inoculation had an aroma like lactic acid. In the multiple comparison test, the aroma of co-inoculated tuak was better with a moderate level of difference. The hedonic test showed that panelists preferred the aroma of co-inoculated tuak, specifically with a yeast to suspected as LAB ratio of 1:2. The alcohol levels of the tuak under these conditions were 0.94% ± 0.06 with pH 3.95 ± 0.03.